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All Journal MEDIA MEDIKA INDONESIANA Molucca Medica Jurnal Pendidikan Kimia, Fisika dan Biologi
Martha Kaihena
Jurusan Biologi FMIPA Dan Program Pendidikan Dokter Universitas Pattimura Ambon, Jl. Dr. Tamaela, Kampus Universitas Pattimura Ambon
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Induksi Polifenol Mahkota Dewa dan Apoptosis Sel Kanker Paru Mencit Strain Balb/C: Analisis pada Up-Regulation Bax dan Down-Regulation Bcl-2 Theopilus W Watuguly; Tjahjono Tjahjono; Martha Kaihena; Syahran Wael
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 1 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1798.474 KB)

Abstract

Mahkota Dewa polyphenol induction and apoptosis cell lung cancer in Balb/c strain mice analysis on up-regulation bax and downregulation Bcl-2Background: This research is aimed to prove the role of Mahkota Dewa polyphenol in the up-regulation Bax and down-regulation in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Method: Post test control group design with 30 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice inducted with BaP and then randomized into 2 groups, which were the control group and the treatment group (polyphenol 50mg). The development of the lung carcinogenesis was observed by tissue surgery in the 8th, 17th and 26th week. The data collected include expression examination Bax and Bcl-2. The data analysis was conducted by Kruskal-Wallis, Mann-Whitneyand correlation test of Spearman's with significance degree of p<a (0.05).Result: The oral administration of Mahkota Dewa polyphenol of 50 mg were significantly decreased the occurance of lung carcinogenesis through decreasing of protein Bax and increasing Bcl-2 in treatment group in week 8, 17 and 26 (p=0.000). Protein Bax for the control group for week 8 were 4.04±0.22 and 1.92±0.10 in week 26, while the treatment group were 5.96±0.32 and 4.68±0.22 (p=0.000). Protein Bcl-2 for the control group for week 8 were 5.80±0.32 and 9.64±0.26 in week 26, while the treatmentgroup were 5.12±0.22 and 7.38±0.21 (p=0.000). The Spearman correlation for Bax and Bcl-2 with significancy value of (p=0.000).Conclusion: The administration of Mahkota Dewa polyphenol of 50 mg effectively increased Bax protein expression and decreased Bcl-2 protein expression in mice Balb/c.Keywords: Polyphenol, Mahkota Dewa (Phaleria macrocarpa [Scheff.] Boerl), protein Bax, protein Bcl-2, strain Balb/c mice, benzo(a)pyrene (BaP)ABSTRAKLatar belakang: Penelitian ini bertujuan membuktikan peran polifenol Mahkota Dewa dalam meningkatkan protein Bax dan menurunkan protein Bcl-2 pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Post test only control group design dengan sampel 30 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 gram, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan karsinogenesis paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi pemeriksaan ekspresi Bax dan Bcl-2. Analisis data dengan Kruskal-Wallis dilanjutkan denganuji Mann-Whitney dan uji korelasi Spearman dengan derajat kemaknaan p<a (0,05).Hasil: Pemberian oral polifenol Mahkota Dewa sebesar 50 mg secara bermakna memperlihatkan peningkatan protein Bax dan penurunan protein Bcl-2 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Protein Bax pada kelompok kontrol minggu ke-8 sebesar 4,04±0,22 dan 1,92±0,10 minggu ke-26, sedangkan kelompok perlakuan sebesar 5,96±0,32 dan 4,68±0,22 (p=0,000) sedangkan protein Bcl-2 pada kelompok kontrol minggu ke-8 sebesar 5,80±0,32 dan 9,64±0,26 minggu ke-26, sedangkan kelompok perlakuan sebesar 5,12±0,22 dan 7,38±0,21 (p=0,000). Hasil uji korelasi Spearman protein Bax dan protein Bcl-2 dengan nilai significancy (p=0,000).
EFEKTIVITAS EKSTRAK METANOL KULIT BATANG KAYU MANIS TERHADAP PENURUNAN KADAR GLUKOSA DARAH DAN REGENERASI SEL-β PANKREAS PADA MODEL MENCIT DIABETES Martha Kaihena; Titus Frendi Wedilena; Suyono Lateke; Maria Nindatu
Molucca Medica VOLUME 12, NOMOR 2, OKTOBER 2019
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (780.885 KB) | DOI: 10.30598/molmed.2019.v12.i2.10

Abstract

Pendahuluan. Penggunaan obat herbal telah digunakan secara turun-temurun karena selain efek sampingnya relatif kecil juga harga lebih ekonomis. Salah satu tumbuhan yang biasa digunakan adalah kayu manis. Tujuan.Penelitian ini bertujuan untuk mengetahui penurunan kadar gula darah dan Perbaikan Pulau Langerhans Mencit (Mus musculus) Diabetes Mellitus Type-I Yang Diterapi ekstrak metanol kulit batang pohon kayu manis (Cinnamomum burmani). Metode. Hewan coba yaitu mencit dengan berat badan 20 – 30 g dibagi dalam 4 kelompok masing- masing: kelompok kontrol positif (K1) , kontrol negatif (K2), kelompok 125 mg/kg BB (K3), kelompok 250 mg/kg BB (K4) dan 500 mg/kg BB (K5). Pada kelompok mencit coba K1, K3, K4, dan K5 diinjeksi dengan streptozotocin dan diamati kadar gula darah selama 2 minggu dan mencit diabetes diberikan ekstrak kayu manis dengan dosis yang telah ditentukan selama 2 minggu. Selanjutnya dilakukan pengukuran kadar gula darah mencit coba serta mencit dibedah dan diambil organ pancreas untuk dilakukan preparasi. Data hasil pengamatan akan dianalisis menggunakan Analisa Varians. Hasil. Hasil penelitian menunjukkan bahwa pemberian ekstrak metanol kulit batang kayu manis dapat menurunkan kadar gula darah mencit ( 71,83 mg/dl) sesudah 2 minggu dan meregenerasi sel β pulau lengerhans pancreas mencit diabetes mellitus. Kesimpulan. Ekstrak metanol kulit batang kayu manis dosis 500 mg/kg BB per hari efektif dalam menurunkan kadar gula darah dan meregenerasi sel β pulau lengerhans pancreas mencit diabetes mellitus. Kata Kunci : Diabetes Mellitus, Kulit Batang Kayu Manis, Regenerasi sel β Pankreas
Aktivitas Farmakologis Ekstrak Kulit Batang dan Buah Garcinia mangostana L. terhadap Percepatan Penyembuhan Luka Sayat pada Mencit (Mus musculus) Abdul M Ukratalo; Martha Kaihena; Efraim Samson
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 1 No. 4 (2025): Juli: Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v1i4.425

Abstract

Wounds are damage to body tissues caused by various factors, both external and internal, which can disrupt the normal function of these tissues. Many studies have focused on using natural ingredients as adjunctive therapy to accelerate wound healing. One plant that shows therapeutic potential is Garcinia mangostana L. This study aims to assess the therapeutic potential of Garcinia mangostana L. stem bark and fruit peel extracts in accelerating the healing of mice (Mus musculus) incision wounds. The study used a completely randomized design (CRD) with six treatments and three replicates, namely negative control mice (K-), positive control (K+), groups of mice given fruit peel and stem bark extracts of G. mangostana concentrations of 25, 50, 75 and 100%. All mice were cut with a wound diameter of 1 cm. The wounds were then left for 13 days with an interval of 3 days for measurement. The results showed that the administration of G. mangostana fruit peel and stem bark extracts had an effect in accelerating the healing of mice incision wounds. From these results, Garcinia mangostana L. has the potential as an additional therapy in accelerating wound healing in mice, which can be used as a basis for further research related to its application in humans.
Co-Authors Abdul M Ukratalo Efraim Samson Nindatu, Maria Suyono Lateke Syahran Wael Theopilus W Watuguly Titus Frendi Wedilena Tjahjono Tjahjono