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All Journal BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
GINA KARTINA
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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EFEKTIVITAS EKSTRAK BIJI PICUNG (Pangium edule Reinw.) TERHADAP MORTALITAS KEONG MAS (Pomacea canaliculate Lamck.) Yuningsih, Yuningsih; Kartina, Gina
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.943 KB) | DOI: 10.14203/beritabiologi.v8i4.2121

Abstract

This research attempted to substitute commercial with botanical molluscicide i.e. picung (Pangium edule) seed. The trial of picung seed water extract (PWE) was conducted in 3 groups of gold apple snails (Pomacea canaliculata) - (GAS).Group 1 with body weight (bw) was 4 to 5 grams, Group 2 was 10 to 12 grams and Group 3 was blank. Both group (1 and 2) were soaked in 4 regimes of cyanide concentration from PWE: 100, 50, 25 and 12.5 /tg/mi and put 10 snails for each concentration. The observation on their mortality was conducted for 24 hours. The result showed that PWE killed 100% of GAS (< 1 hour) with concentration in 50 µg/ml and 25 µ g/ml cyanide in Group 1 and Group 2, respectively, with clinical sign is the production of excessive mucous as criteria of molluscicide effect thus picung seed is quite significant as botanical molluscicide.
AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN PEPTIDA SUSU KAMBING HASIL HIDROLISIS DENGAN PROTEASE LACTOBACILLUS PLANTARUM S31 Herlina, Nina; Mustopa, Apon Zaenal; Surachma, Rahma Sari; Triratna, Lita; Kartina, Gina; Alfisyahrin, Wida Nurul
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3762

Abstract

ABSTRACTResearch on the hydrolysis of milk with various protease obtained from the digestive tract, plants and animals has been widely carried out, but the hydrolysis process of goat milk with protease enzymes from Lactobacillus plantarum S31 isolated from bekasam is still widely unknown. This study aims to determine the antibacterial and antioxidant activity of peptides hydrolyzed by protease enzymes from L. plantarum S31. Isolation and purification of extracellular proteases was carried out with 80% ammonium sulfate saturation, dialysis and G50 Sephadex matrix. The results of hydrolysis showed that fraction 11 has the highest antioxidant activity. Goat milk with pH 5 has a greater inhibitory activity of about 29%, which is 5% compared to goat milk pH 7. This fraction also has a quite good antibacterial activity of Entero Phatogenic Escherichia coli (EPEC K1.1) bacteria, Staphylococcus aureus and Listeria monocytogenes.  Keywords: goat milk, hydrolysis, antibacterial, antioxidant, Lactobacillus plantarum
AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN PEPTIDA SUSU KAMBING HASIL HIDROLISIS DENGAN PROTEASE LACTOBACILLUS PLANTARUM S31 Herlina, Nina; Mustopa, Apon Zaenal; Surachma, Rahma Sari; Triratna, Lita; Kartina, Gina; Alfisyahrin, Wida Nurul
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3762

Abstract

ABSTRACTResearch on the hydrolysis of milk with various protease obtained from the digestive tract, plants and animals has been widely carried out, but the hydrolysis process of goat milk with protease enzymes from Lactobacillus plantarum S31 isolated from bekasam is still widely unknown. This study aims to determine the antibacterial and antioxidant activity of peptides hydrolyzed by protease enzymes from L. plantarum S31. Isolation and purification of extracellular proteases was carried out with 80% ammonium sulfate saturation, dialysis and G50 Sephadex matrix. The results of hydrolysis showed that fraction 11 has the highest antioxidant activity. Goat milk with pH 5 has a greater inhibitory activity of about 29%, which is 5% compared to goat milk pH 7. This fraction also has a quite good antibacterial activity of Entero Phatogenic Escherichia coli (EPEC K1.1) bacteria, Staphylococcus aureus and Listeria monocytogenes.  Keywords: goat milk, hydrolysis, antibacterial, antioxidant, Lactobacillus plantarum
Co-Authors Alfisyahrin, Wida Nurul ANDO KATSUHIKO Apon Zaenal Mustopa Lita Triratna Nina Herlina PUSPITA LISDIYANTI RONI RIDWAN SHANTI RATNAKOMALA Surachma, Rahma Sari TOMOHIKO TAMURA Yantyati Widyastuti YULIN LESTARI Yuningsih Yuningsih