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Sintesis dan Uji Aktivitas Antibakteri Senyawa N-fenil-4-klorobenzamida Elok Dea Orens Ubung Wisnu; Indah Purnama Sary; Dwi Koko Pratoko
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7569

Abstract

N-phenylbenzamide is benzamide derivatives, which is potential as an antibacterial agent. N-phenyl-4-chlorobenzamide is N-phenylbenzamide derivative that substitued by chloro to the para position and it was expected to enhance the antibacterial activity. N-phenyl-4-chlorobenzamide was synthesized by reacting 1,3-diphenyilthiourea and 4-chlorobenzoil chloride. This compound has been purified and provided 53% of product with crystal shape, white color, and melting point of 195-197 oC. The purification of this compound was confirmed by TLC and the structure was identified by 1H-NMR, 13C-NMR, and FTIR spectroscopy. This compound was tested for its activity against gram positive bacteria Staphylococcus aureus and gram negative bacteria Pseudomonas aeruginosa, evaluated by well diffusion method and the result showed no activity against both Staphylococcus aureus and Pseudomonas aeruginosa. Keywords: N-phenyl-4-chlorobenzamide, synthesis, antibacterial activity
Pengembangan Biosensor Berbasis Kertas untuk Penentuan Aktivitas Antihiperlipidemia Pada Sampel Ekstrak Tanaman Vinda Aisya Vira; Bambang Kuswandi; Indah Purnama Sary
Pustaka Kesehatan Vol 9 No 2 (2021): Volume 9 No.2, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i2.19051

Abstract

A biosensor is an alternative method to determine the antihyperlipidemic activity to be more simple, effective, and efficient in various herbal extracts. This study aimed to develop a biosensor with the principle of pancreatic lipase enzyme inhibition in paper microzone plates using Whatman filter paper for the matrix, 200 unit/mL of lipase enzyme solution, 1% extract, and 80 mM p-NPB substrate. This paper-based biosensor method shows results that fulfilled the several characterizations, that are the 30 min of the incubation time; linearity using 7 standards orlistat concentrations of 5-35 mg/mL with the regression equation of Y= 22.09585 + 2.170514X, correlation coefficient value (r)= 0.9996745, Vx0= 1.509906%, Xp= 1.572533 mg/mL; LOD and LOQ are 1,573 mg/mL and 4,718 mg/mL; precision with RSD value of 2.545%; accuracy with % recovery value of 99.222%; biosensor usage time after storage remained stable for 3 days at chiller temperatures and for 45 minutes at room temperature. The paper-based biosensor can be an alternative method for detecting antihyperlipidemic activity in plant extracts as same as UV-Vis spectrophotometry method.
Sintesis dan Uji Aktivitas Antibakteri Senyawa N-Fenil-4-Bromobenzamida (Synthesis and Antibacterial Activity Assay of N-Phenyl-4-Bromobenzamide) Nazilatul Maghfiroh; Indah Purnama Sary; Dwi Koko Pratoko
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i2.5868

Abstract

Infectious diseases are contagious disease caused by pathogenic microorganisms and can be spread directly or indirectly from one person to another. The aim of this research was to synthesized N-phenyl-4-bromobenzamide as a new antibacterial agent. Thereaction was carried out by reacting 1,3-diphenylthiourea with 4-bromobenzoyl chloride by nucleophilic substitution. The purification of this compound was confirmed by TLC and the structure was identified by UV, IR, 1H-NMR and 13C-NMR. The newly synthesized compound was screened for its antibacterial activity against gram-positive and gram-negative bacteria, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 using well diffusion method. The concentration of the test solution used were 31.25; 62.5; 125; 250; 500; 1,000; 2,000; 4,000; 8,000; and 10,000 μg/ml with levofloxacin as positive control. The synthesized product did not show any antibacterial activity which characterized by the absence of inhibition zone.Keywords: 1,3-diphenylthiourea, N-phenyl-4-bromobenzamide, nucleophilic substitution, antibacterial activity.
Sintesis dan Uji Aktivitas Antibakteri Senyawa N-Fenil-3,4-Diklorobenzamida (Synthesis and Antibacterial Activity Assay of N-Phenyl-3,4-Dichlorobenzamide) Vinastika Gita Yualanda; Indah Purnama Sary; Dian Agung Pangaribowo
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.6610

Abstract

In this research, the synthesis of N-phenyl-3,4-diklorobenzamida was carried out by reacting 1,3-diphenylthiourea and 3,4-dichlorobenzoil chloride via bimolecular nucleophilic substitution reaction (SN2). The assays for the obtained compound product were purity test (tested by TLC and melting point tester), chemical and physical characterization (shape, color, and solubility), confirmation of the compound and structure (performed by UV-Vis spectrophotometer, FTIR spectrometer, 1H-NMR and 13C-NMR). The result showed that the compound was synthesized successfully with a yield of 42%, then the in vitro test of antibacterial activity against Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 was conducted by well diffusion method. The compound N-phenyl-3,4-dichlorobenzamida did not have antibacterial activity which showed by the absence of inhibition diameter. Keywords: 1,3-diphenylthiourea, N-phenyl-3,4-dichlorobenzamide, nucleophilic substitution, antibacterial
Pengembangan Sensor Kimia Berbasis Strip Tes untuk Deteksi Logam Berat Merkuri pada Sampel Produk Jamu Rizqi Amaliyah; Nia Kristiningrum; Indah Purnama Sary
Pustaka Kesehatan Vol 9 No 1 (2021): Volume 9 No.1, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i1.16552

Abstract

The use of plants as a traditional medicine in the modern era is still in high demand. One of the herbs commonly used by Indonesian is analgesic herbal medicine. The safety aspect of herbal medicine should be considered, such as heavy metal contamination. Mercury is the typical heavy metal found in polluting herbal plants. The maximum mercury level allowed in a medicinal preparation has been regulated by BPOM RI, as much as ≤ 0.5 ppm. A high level of mercury contamination causing toxic effects on the body due to protein precipitation, which inhibits enzyme activity and acts as a corrosive material. A technology that allows the detection of mercury is a chemical sensor. The strip test is optimal if the reagent 1-(2-thiazolyl azo)-2-naphthol (TAN) concentration at 1000 ppm and the sample has been confirmed at pH 7. The results of the strip test characterization showed that the response time was in the range of minutes 17 to 24, the use time of the test strips for 28 days if stored in room temperature and 29 days if stored at in refrigerator temperature, linearity with r = 0.998, Vx0 = 3.96%, p-value = 0.00000172, LOD = 0.102 ppm and LOQ = 0.306 ppm, interference value <5%, precision with RSD 6.54%, and accuracy with the value of % recovery 98.007%. T-test analysis showed p= 0.872, meaning no significant difference between the application of TAN-based test strips with the ICP-AES method.
Co-Authors Bambang Kuswandi Dian Agung Pangaribowo Dwi Koko Pratoko Elok Dea Orens Ubung Wisnu Nazilatul Maghfiroh Nia Kristiningrum Rizqi Amaliyah Vinastika Gita Yualanda Vinda Aisya Vira