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All Journal Jurnal Kedokteran Brawijaya Majalah Kesehatan FKUB Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia)
Kusnarman Keman
Laboaratorium Obstetri Ginekologi Fakultas Kedokteran Universitas Brawijaya/ RS. Dr. Saiful Anwar
Health Professions Medicine & Pharmacology Public Health

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Fetal Fibronectin (fFN) on the Imminent Premature Parturition in Correlation with Incidence of Preterm Labor Keman, Kusnarman; Nugrahanti, Prasetyorini; Supriany, Ni Wayan
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 1, January 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.179 KB) | DOI: 10.32771/inajog.v4i1.65

Abstract

Objective: To determine the relationship of fFn levels in cervicovaginal discharge of pregnant women who experience imminent premature parturition with the incidence of preterm labor. Method: The study was carried out with Analytic Observational Prospective Cohort using cervicovaginal discharge of pregnant women that experienced imminent premature parturition taken from the delivery room of Obstetrics and Gynecology department dr. Saiful Anwar Hospital, Malang as well as Bangil Hospital and Ngudi Waluyo Wlingi Hospital. Statistical analysis was performed using the Shapiro-Wilk test and comparison test used independent samples t test for normal data, Mann-Whitney test if not. All analysis used SPSS for Windows 19.0 software. Result: Thirty two patient samples was examined, 14 patients (43.75%) were primigravida and 18 patients (52.56%) is multigravida. 17 of these patients (53.13%) experienced aterm labor and 15 patients (46.87%) experienced preterm labor. Mann-Whitney test of the mean fFN levels between the aterm group (13.01 ± 7.57 ng/ml) and the preterm group (56.29 ± 27.77 ng/ml) showed a significant difference (p-value = 0.000 > 0.05). Moreover, Spearman’s Rho correlation test also showed a strong correlation between fFN level and incidence of preterm labor (R = 0.797, p < 0.05). Conclusion: fFN levels is significantly increase in cervicovaginal discharge from pregnant women with imminent premature parturition who experience preterm labor than pregnant women who experience aterm labor. Therefore, this result suggests that fFN has potential ability to become useful modality in preterm labor diagnosis. [Indones J Obstet Gynecol 2016; 1: 8-14] Keywords: cervicovaginal discharge, fFN, imminent premature parturition, preterm labor
Fetal Fibronectin (fFN) on the Imminent Premature Parturition in Correlation with Incidence of Preterm Labor Keman, Kusnarman; Nugrahanti, Prasetyorini; Supriany, Ni Wayan
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 1, January 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.179 KB) | DOI: 10.32771/inajog.v4i1.65

Abstract

Objective: To determine the relationship of fFn levels in cervicovaginal discharge of pregnant women who experience imminent premature parturition with the incidence of preterm labor. Method: The study was carried out with Analytic Observational Prospective Cohort using cervicovaginal discharge of pregnant women that experienced imminent premature parturition taken from the delivery room of Obstetrics and Gynecology department dr. Saiful Anwar Hospital, Malang as well as Bangil Hospital and Ngudi Waluyo Wlingi Hospital. Statistical analysis was performed using the Shapiro-Wilk test and comparison test used independent samples t test for normal data, Mann-Whitney test if not. All analysis used SPSS for Windows 19.0 software. Result: Thirty two patient samples was examined, 14 patients (43.75%) were primigravida and 18 patients (52.56%) is multigravida. 17 of these patients (53.13%) experienced aterm labor and 15 patients (46.87%) experienced preterm labor. Mann-Whitney test of the mean fFN levels between the aterm group (13.01 ± 7.57 ng/ml) and the preterm group (56.29 ± 27.77 ng/ml) showed a significant difference (p-value = 0.000 > 0.05). Moreover, Spearman’s Rho correlation test also showed a strong correlation between fFN level and incidence of preterm labor (R = 0.797, p < 0.05). Conclusion: fFN levels is significantly increase in cervicovaginal discharge from pregnant women with imminent premature parturition who experience preterm labor than pregnant women who experience aterm labor. Therefore, this result suggests that fFN has potential ability to become useful modality in preterm labor diagnosis. [Indones J Obstet Gynecol 2016; 1: 8-14] Keywords: cervicovaginal discharge, fFN, imminent premature parturition, preterm labor
JUMLAH SEL TROFOBLAS YANG MENGALAMI APOPTOSIS PADA PRE EKLAMPSIA/EKLAMPSIA LEBIH TINGGI DIBANDINGKAN KEHAMILAN NORMAL Keman, Kusnarman; Prasetyorini, Nugrahanti; Langgar, Madeline J.
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1335.644 KB) | DOI: 10.21776/ub.jkb.2008.024.02.8

Abstract

Hypoxia exposed trophoblast cells will cause apoptotic process, that correlate with up-regulation of p53 and Bax protein expression; and down-regulation of anti-apoptotic Bcl-2 protein. Apoptotic process and up-regulation of p52, and down-regulation of anti-apoptotic Bcl-2; also find both on trophoblastic samplefrom preeclampsia/ eclampsia complicated pregnancy, and  normal pregnancy. The aim of this study is, to prove that the amount of apoptotic trophoblast cells on trophoblast tissue sample from preeclampsia/ ecclampsia complicated pregnancy, were higher then those of normal pregnancy. This research was laboratory cross sectional study, that observed immunohystochemically, p53 and BCl-2 protein expression; also the DNA-fragmented technically (TUNEL) to detect apoptotic  cells on trophoblastic samples from preeclampsia/ eclampsia complicated pregnancy, compare by the normal pregnancy. On this study, we found that the amount of trophoblast cells that expressed of p53 protein (22.70 ± 2.90), were higher then those of normal pregnancy. (8.2 ± 4.99). The different was statistically significant. (t test; p  ≤0.000). We also found that an apoptotic process on trophoblast cells of preeclampsia/ eclampsia placenta (15.3 ± 3,56) were higher  then those of normal pregnancy (4.7 ± 1.89). The different was statistically significant. (t test; p ≤0.000).
POLIMORFISME VARIAN T-334C GEN FAKTOR TRANSKRIPSI STORKHEAD BOX 1 (STOX1) SEBAGAI KANDIDAT BIOMARKER PREEKLAMPSIA Nooryanto, Mukhamad; Keman, Kusnarman; Dahlan, Erry Gumilar; Indriani, Anin
Majalah Kesehatan FKUB Vol 8, No 1 (2021): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.majalahkesehatan.2021.008.01.2

Abstract

Insiden preeklampsia dan eklamsia dapat menyebabkan kematian pada ibu hamil, dan pada wanita dengan riwayat keluarga menderita preeklampsia lebih berisiko dibandingkan dengan wanita tanpa riwayat keluarga dengan preeklampsia. Penelitian terdahulu mengindikasikan bahwa gen Storkhead Box 1 (STOX1) merupakan salah satu penyebab preeklampsia. Penelitian ini bertujuan untuk menganalisis polimorfisme varian T-344C gen STOX1 dalam kromosom 10q22 sebagai kandidat biomarker preeklampsia. Penelitian dilaksanakan secara kuantitatif observasional dengan desain cross-sectional pada sampel darah wanita hamil dengan preeklampsia (kelompok kasus) dan darah wanita yang hamil normal (kelompok kontrol) di kamar bersalin SMF Obstetri dan Ginekologi RSUD Dr. Saiful Anwar Malang. Sampel darah vena diambil dari 25 wanita hamil dengan preeklampsia dan 25 wanita hamil normal yang memenuhi kriteria penelitian dan dilakukan ekstraksi DNA. Identifikasi gen STOX1 dilakukan dengan metode  PCR dengan forward primer adalah 5’-TGGGTGTGAA-3’ dan reversed primer 5’-TTGGAGCGTTTGATGAAACA-3’ (First Base, USA) dan analisis varian T-344C dengan RFLP menggunakan enzim HaeIII. Data dianalisis secara statistik dengan aplikasi SPSS. Amplifikasi gen STOX1 dengan PCR pada kelompok preeklamsia dan kontrol menunjukkan  produk sebesar 639 bp. Analisis polimorfisme varian T-334C pada kelompok normal memiliki frekuensi TT 64%, TC 28%, dan CC 8%. Sementara pada kelompok preklampsia frekuensi TT 72%, TC 28%)dan tidak ditemukan adanya polimorfisme CC yang bersifat homozigot. Tidak ditemukan hubungan yang signifikan antara polimorfisme varian T-334C gen STOX1 dengan preeklampsia (Uji Mann Whitney p = 0,668 > 0,05). Kesimpulanya, secara teknis pemeriksaan varian T-344C gen STOX1 dapat digunakan sebagai kandidat biomarker preeklamsia, namun secara spesifik tidak sensitif untuk menjelaskan adanya hubungan antara polimorfisme varian T-344C gen STOX1 dengan preeklampsia. 
Co-Authors Dahlan, Erry Gumilar Indriani, Anin Madeline J. Langgar Mukhamad Nooryanto, Mukhamad Nugrahanti Prasetyorini Nugrahanti, Prasetyorini Supriany, Ni Wayan