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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Yuyun Lusini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Journal : Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

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Aktivitas Lipase Rhizopus microsporus var. rhizopodiformis UICC 520 dan Rhizopus microsporus var. oligosporus UICC 550 pada Substrat Minyak Nabati Wibowo Mangunwardoyo; Yuyun Lusini; Indrawati Gandjar
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.57

Abstract

Dua biakan micro-Rhizopus ditumbuhkan pada substrat minyak nabati untuk meneliti kemampuan memproduksi lipase ekstraselular. Medium basal ditambahkan minyak olive, kelapa, jagung, bunga matahari dan minyak kedelai. Aktivitas lipase diuji dengan metode titrasi. Aktivitas lipase tertinggi pada substrat minyak olive pada R. microsporus var. oligosporus UICC 550 (16,23 U/g Biomassa) dan minyak kelapa pada R. microsporus var. rhizopodiformis UICC 520 (30,5 U/g Biomassa) setelah 48 jam inkubasi. Nilai pH mengalami kenaikan selama berlangsungnya proses fermentasi.
Karakterisasi, Pengaruh Sumber Nitrogen dan Karbon terhadap Produktivitas Enzim Lipase Rhizopus microsporus var oligosporus UICC 550 Wibowo Mangunwardoyo; Yuyun Lusini; Indrawati Gandjar
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2689

Abstract

Effect of nitrogen and carbon source on the production of extracellular lipase by R.microsporus var. oligosporus UICC 550 was studied. The enzyme activity was alsocharacterized in terms of temperature, pH, stability at room temperature, and effectof divalent ion. The amount of 2.5% (w/v) of olive oil as carbon sources and 5% (w/v)peptone as nitrogen sources were the optimum for production of lipase enzyme.Partial purifications using ammonium sulfate followed by dialysis showed that at pH6.5 and temperature 35oC was the optimum condition, respectively. The stability(remaining up to 80% of the optimum enzyme) was recorded at pH 6.5 after 24 hoursincubation at room temperature. The optimum activity remained 40% of the after onehour incubation at 35oC. Divalent ions concentration at 1 mM, Zn2+, Cu2+, Mg2+ andFe2+ inhibited the lipolytic activity.
Co-Authors Anak Agung Gede Sugianthara Destri Destri Indrawati Gandjar Lily Ismaini Mirwan Abu Bakar Sidiq Muhammad Imam Surya Nurul Asni Siti Suhartati Suluh Normasiwi Wibowo Mangunwardoyo