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All Journal Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Indrawati Gandjar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Aktivitas Lipase Rhizopus microsporus var. rhizopodiformis UICC 520 dan Rhizopus microsporus var. oligosporus UICC 550 pada Substrat Minyak Nabati Wibowo Mangunwardoyo; Yuyun Lusini; Indrawati Gandjar
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.57

Abstract

Dua biakan micro-Rhizopus ditumbuhkan pada substrat minyak nabati untuk meneliti kemampuan memproduksi lipase ekstraselular. Medium basal ditambahkan minyak olive, kelapa, jagung, bunga matahari dan minyak kedelai. Aktivitas lipase diuji dengan metode titrasi. Aktivitas lipase tertinggi pada substrat minyak olive pada R. microsporus var. oligosporus UICC 550 (16,23 U/g Biomassa) dan minyak kelapa pada R. microsporus var. rhizopodiformis UICC 520 (30,5 U/g Biomassa) setelah 48 jam inkubasi. Nilai pH mengalami kenaikan selama berlangsungnya proses fermentasi.
Karakterisasi, Pengaruh Sumber Nitrogen dan Karbon terhadap Produktivitas Enzim Lipase Rhizopus microsporus var oligosporus UICC 550 Wibowo Mangunwardoyo; Yuyun Lusini; Indrawati Gandjar
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2689

Abstract

Effect of nitrogen and carbon source on the production of extracellular lipase by R.microsporus var. oligosporus UICC 550 was studied. The enzyme activity was alsocharacterized in terms of temperature, pH, stability at room temperature, and effectof divalent ion. The amount of 2.5% (w/v) of olive oil as carbon sources and 5% (w/v)peptone as nitrogen sources were the optimum for production of lipase enzyme.Partial purifications using ammonium sulfate followed by dialysis showed that at pH6.5 and temperature 35oC was the optimum condition, respectively. The stability(remaining up to 80% of the optimum enzyme) was recorded at pH 6.5 after 24 hoursincubation at room temperature. The optimum activity remained 40% of the after onehour incubation at 35oC. Divalent ions concentration at 1 mM, Zn2+, Cu2+, Mg2+ andFe2+ inhibited the lipolytic activity.
Identification and Phylogenetic Analysis of Bacterial Isolates from Litopenaeus vannamei Shrimp Culture System and Gut Environment Based on 16SrRNA Gene Sequence Data TUBAGUS HAERU RAHAYU; INDRAWATI GANDJAR; ETTY RIANI; IIN SITI DJUNAIDAH; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.506 KB) | DOI: 10.5454/mi.3.2.3

Abstract

Selected bacterial isolates from a Litopenaeus vannamei shrimp culture system and gut environment were assessed using 16S rRNA gene sequencing method to identify their identity and to construct their phylogenetic relationship. In a preliminary study, a total of 19 isolates were selected as probiotics. These isolates were prepared using freeze and heat-shock method to obtain the DNA template. PCR amplification of 16S ribosomal RNA gene of isolates was carried out using bacterial universal primers 9F and 1510R and was sequenced using an automated DNA sequencer. These gene sequences were compared with other gene sequences in the GenBank database (NCBI) using a BLAST search to find closely related sequences. Alignment of these sequences with sequences available from GenBank database was carried out to construct a phylogenetic tree for these bacteria. Most of the isolates obtained, i.e. 17 out of the 19 isolates, belonged to different species of Bacillus, sharing 95 to 99% 16S ribosomal RNA identity with the respective type-strain, whereas the remaining 2 isolates belonged to Micrococcus sp. and Micrococcus luteus with 97 to 99% 16S rRNA homology, consecutively.
Co-Authors Etty Riani IIN SITI DJUNAIDAH TUBAGUS HAERU RAHAYU Wellyzar Sjamsuridzal Wibowo Mangunwardoyo Yuyun Lusini