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All Journal PROSIDING SEMINAR NASIONAL Biospecies Jurnal Ilmu Lingkungan Biogenesis: Jurnal Ilmiah Biologi Prosiding Jurnal Akuatika Indonesia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal La Lifesci JURNAL VOKASI ILMU-ILMU PERIKANAN (JVIP) Eduvest - Journal of Universal Studies
Moh. Husein Sastranegara
Universitas Jenderal Soedirman
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Neuroscience Social Sciences Veterinary

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DIVERSITAS IKAN INTRODUKSI DAN INDIGENUS DI SUNGAI BANJARAN DAN PELUS KABUPATEN BANYUMAS Lestari, W.; Sastranegara, Moh. Husein
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Ikan diintroduksi dengan tujuan untuk budidaya seperti Clarias gariepinnis, Oreochromis mossambicus dan Cyprinus carpio. Kegiatan ini diperlukan untuk memenuhi kebutuhan protein hewani, untuk pengendali hama dan penyakit seperti Puntius reticula dan Aquidens latifrons dan sebagai ikan hias seperti Aquidens pulcher. Namun, kehadiran introduced species pada perairan menyebabkan penurunan populasi, kepunahan dan diversitas indigenous species. Hal ini terjadi karena pemangsaan, kompetesi dalam mendapatkan habitat dan pakan, gangguan dalam mendapatkan pasangan dan penyebaran bakteri patogen serta kawin silang antara introduced dan indigenous species. Penelitian ini bertujuan untuk mengetahui ikan-ikan introduksi dan indigenus , modus penyebaran dan model pencegahannya. Materi yang digunakan adalah ikan- ikan introduced dan indigenous species, isi perut ikan, plankton serta air dari Sungai Banjaran dan Sungai Pelus, Kabupaten Banyumas. Metode yang digunakan adalah metode survai dengan purposive random sampling. Hasi Penilitian menunjukkan 8 spesies ikan introduksi yaitu Oreochromis niloticus, Oreochromis mossambicus, Hypostomus plecostomus dan Cyprinus carpio. Oreochromis niloticus dan Oreochromis mossambicus, sebagai ikan budidaya, sementara Xyphophorus hellerii, Hypostomus plecostomus, Pterygoplichthys pardalis dan Cyprinus carpio sebagai ikan hias dan Poecilia reticulata sebagai pengendalikan nyamuk. Tiga belas indigenous species. Osteochilus hasseltii, Puntius orphoides, Puntius binotatus, Barbonymus gonionotus, Rasbora argyrotaenia, Rasbora lateralistriatas, Glyptothorax platypogon. Nemacheilus fasciatus, Channa gachua, Puntius bramoides, Macrognathus maculates dan Mystus micracanthus. Diversitas dan abundansi introduced species meningkat tajam di lokasi-lokasi dekat aktivitas budidaya. Modus penyebaran ikan ini di Sungai Banjaran dan Pelus karena lepas dari kolam kolam budidaya. Hal ini terkait erat dengan melimpahnya pakan yang diduga berasal dari kolam budidaya. Oleh karena itu, metode pengendalian diversitas ikan introduced species dengan menerapkan alat pencegah lepasnya ikan serta alat penyaring air buangan dari kolam budidaya ke sungai.
Molecular Identification of Fish Larvae from East Plawangan of Segara Anakan, Cilacap, Central Java, Indonesia Nuryanto, Agus; Pramono, Hendro; Sastranegara, Moh Husein
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9191

Abstract

Correct identification of fish larvae from East Plawangan is very difficult to be done due to undefined of their morphology, while correct name is an important information for management of that area as spawning and nersery ground. Therefore, so far no scientific report on what species that utilize East Plawangan as their spawning and nursery ground. Cytochrome c oxidase 1 gene provide a precise tool for correct larvae identification. This study aims to test the accuracy of the cytochrome c oxidase 1 gene on fish larvae identification. The COI gene were amplified and sequenced. The homology of sequences were checked using Basic Local Alignment Search Tool (BLAST). Genetic distances were calculated with the help of MEGA 6 software. Taxonomic tree was reconstructed using neighbor-joining and maximum parsimony methods with 1000 bootstraps repeats. A sum of 24 morphotypes were identified, indicating high diversity of fish that utilize East Plawangan as their spawning and nursery ground. BLAST result showed that seven morphotypes were convincingly identified into species level, while three remaining morphotypes could only be identified at generic level. Taxonomic tree shows clear discrimination among morphotypes. This proved that cytochrome c oxidase 1 gene showed high accuracy on larval species discrimination.
Molecular Characterization of Anguilla from Cibereum and Sapuregel Rivers Segara Anakan Watersheds Cilacap, Central Java Agus Nuryanto; Dian Baghawati; Kusbiyanto Kusbiyanto; Moh Husein Sastranegara; Farida Nur Rachmawati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15532

Abstract

The taxonomic status of Anguilla species' in river watershed that empties into Segara Anakan Cilacap is uncertain, thereby making it difficult for further studies to be carried out to determine its genetic in that area. Therefore, this study evaluates Anguilla's taxonomic status and population genetic in Cibeureum and Sapuregel River watersheds. Data were obtained from molecular characterization study using cytochrome c oxidase 1, with fourteen Anguilla specimens collected from two sequenced watersheds. Taxonomic status was determined based on homology and divergence values and monophyly of the samples to the reference species. Meanwhile, genetic divergences among samples to the reference species were calculated based on the Juke-Cantor substitution model in DnaSP6. A homology test was performed using a basic local alignment search tool, with monophyly inferred from the cladogram, which was developed using neighbor-joining and maximum likelihood algorithms in MEGAX with 1000 pseudoreplicates and out-group comparison. Furthermore, population genetic was analyzed through polymorphism, haplotype, nucleotide diversity within the population, divergence, and genetic differences. All calculations conducted in Arlequin 3.5 had Anguilla samples comprising of high (99.23% to 99.84%) to low genetic divergences (0.224% to 1.127%). The result shows that cladogram with all Anguilla samples formed a monophyletic clade with A. bicolor, separated from their taxa. Furthermore, Anguilla samples from both watersheds have low genetic polymorphisms with medium to high haplotype and nucleotide diversity. The population's comparison proved that both populations have low genetic divergence, and no genetic difference based on variance analysis (p=0761). Therefore, Anguilla resources in river watersheds that empty into Segara Anakan are a single genetic conservation unit.
PENGARUH FAKTOR LINGKUNGAN FISIK RUMAH DAN PERILAKU PENDERITA TERHADAP KEJADIAN PENULARAN TUBERCULOSIS PARU DI WILAYAH PUSKESMAS WINDUAJI KABUPATEN BREBES Luthfi Hidayat Maulana; Moh. Husein Sastranegara; Budi Aji
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: PROSIDING IMPLEMENTASI PENELITIAN PADA PENGABDIAN MENUJU MASYARAKAT MANDIRI BERKEMAJUAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tuberculosis (TB) is still a top health problem in the world. TB causes health problems Millions of people per year, and was ranked as the prayer Into Deaths Top causes of infectious diseases at the World taxable income HIV. One indicator used is the Case Detection Rate (CDR). CDR is the proportion of new patients Basil Hold acid (BTA) were found positive and the treatment on the number of new smear-positive patients,which is estimated in the region. The study aims to describe the physical environmental factors homes (lighting) patients with pulmonary tuberculosis, describing the physical environmental factors house (moisture) patients with pulmonary tuberculosis, describing behavioral factors patient pulmonary tuberculosis and analyze the influence of the physical environment of the home patient pulmonary tuberculosis and behavior of patients with pulmonary tuberculosis together -Same the incidence of transmission in the family. The study used survey method with cross sectional approach, the sample to be studied is the total population, ie all patients with pulmonary tuberculosis family as much as 47 respondents. The analysis is univariate analysis aimed at describing the characteristics of each study variable through frequency distribution table, the physical environment and behavioral factors.Followed by multivariate analysis to determine the effect of physical environmental factors homes pulmonary tuberculosis patients and patients with pulmonary tuberculosis behavior together on the incidence of transmission in the family. The results showed that the respondents lighting of the room against pulmonary tuberculosis transmission in most families is the category of poor lighting as many as 23 home patients (48.9%), good lighting category as many as 20 home patients (42.6%), and excellent lighting category by 4 home patients (8.5%); respondents humidity of the room to the transmission of pulmonary tuberculosis in most families is the category of dry humidity as many as 25 home patients (53.2%), the category of normal humidity as many as 19 home patients (40.4%), and the category of wet moisture as much as 3 home patients (6, 4%); respondent behavior in the transmission of pulmonary tuberculosis in most families is the category of behavior not good by 32 patients (68.1%), and the category of good conduct as many as 16 patients (1.9%); physical environmental factors homes pulmonary tuberculosis patients in the form of humidity is more influential than the physical environmental factors such as lighting homes and pulmonary tuberculosis patient behavior in the transmission of the family. Keywords: physical environmental factors homes, behavioral pulmonary tuberculosis patients, and the incidence of transmission.Tuberculosis (TB) masih merupakan masalah kesehatan utama di dunia. tuberculosis menyebabkan gangguan kesehatan jutaan orang per tahun dan menduduki peringkat ke dua sebagai penyebab utama kematian akibat penyakit menular di dunia setelah HIV. Salah satu indikator yang digunakan adalah Case Detection Rate (CDR). CDR adalah jumlah proporsi pasien baru Basil Tahan Asam (BTA) positif yang ditemukan dan pengobatan terhadap jumlah pasien baru BTA positif, yang diperkirakan dalam wilayah tersebut. Penelitian bertujuan untuk mendiskripsikanfaktor lingkungan fisik rumah (pencahayaan) penderita tuberculosis paru, mendiskripsikan faktor lingkungan fisik rumah (kelembaban) penderita tuberculosis paru, mendiskripsikan faktor perilaku penderita tuberculosis paru dan menganalisis pengaruh faktor lingkungan fisik rumah penderita tuberculosis paru dan perilaku penderita tuberculosis paru secara bersama-sama terhadap kejadian penularannya pada keluarga. Penelitian menggunakan metode survei dengan pendekatan Cross Sectional, sampel yang akan diteliti adalah total populasi, yaitu semua keluarga penderita tuberculosis paru sebanyak 47 responden. Analisis yang digunakan adalah analisis univariat bertujuan untuk mendiskripsikan karakteristik masing-masing variabel penelitian melalui tabel distribusi frekuensi, pada faktor lingkungan fisik dan perilaku. Analisis dilanjutkan dengan multivariat untuk mengetahui pengaruh faktor lingkungan fisik rumah penderita tuberculosis paru dan perilaku penderita tuberculosis paru secara bersama-sama terhadap kejadian penularannya pada keluarga.Hasil penelitian menunjukkan bahwa responden pencahayaan ruangan terhadap penularan tuberculosis paru pada keluarga terbanyak adalah katagori pencahayaan kurang sebanyak 23 rumah penderita (48,9%), katagori pencahayaan baik sebanyak 20 rumah penderita (42,6%), dan katagori pencahayaan sangat baik sebanyak 4 rumah penderita (8,5%); responden kelembaban ruangan terhadap penularan tuberculosis paru pada keluarga terbanyak adalah katagori kelembaban kering sebanyak 25 rumah penderita (53,2%), katagori kelembaban normal sebanyak 19 rumah penderita (40,4%), dan katagori kelembaban basah sebanyak 3 rumah penderita (6,4%); responden perilaku terhadap penularan tuberculosis paru pada keluarga terbanyak adalah katagori perilaku kurang baik sebanyak 32 penderita (68,1%), dan katagori perilaku baik sebanyak 16 penderita (1,9%); faktor lingkungan fisik rumah penderita tuberculosis paru berupa kelembaban lebih berpengaruh dibandingkan dengan faktor lingkungan fisik rumah berupa pencahayaan dan perilaku penderita tuberculosis paru terhadap penularan pada keluarganya. Kata kunci: faktor lingkungan fisik rumah, perilaku penderita tuberculosis paru, dan kejadian penularan.
Hubungan antara Perubahan Suhu Udara Harian, Perilaku Petani dan Keankeragaman Serangga Penyerbuk di Desa Serang Kecamatan Karangreja, Kabupaten Purbalingga Jawa Tengah (The relationship between the Air Temperature Change Daily, Farmer Behavior, and Diver Dwi YULIANI; Moh. Husein SASTRANEGARA
Biospecies Vol. 8 No. 2 (2015): Juli 2015
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v8i2.2512

Abstract

Global warming has threatened Indonesian’s agricultural sector and put the sectorvulnerable to climate change. The changes affect the daily air temperature changes, farmerbehavior, and the diversity of insect pollinators. The study aims to determine the daily changes inair temperature, farmer behavior, and diversity of pollinating insects in the village of Serang,Karangreja, Purbalingga, Central Java; and to analyze the relationship between daily airtemperature changes, farmer behavior, and insect pollinators diversity. The research employedsurvey methods. Samples for the daily air temperature measurement were taken purposeviley. Thediversity of insect pollinators on the three farming type and respondens were selected radmonlywith total respondent 99. The results showed that the average daily air temperature in chili farms ishigher than that in tomato and strawberry farm; farmers have a good knowledge about theenvironmental degradation of agriculture land, good attitude and awareness in maintaining andimproving the quality of agriculture, but they have negative behaviour in the use of excessiveinsecticides. Species richness of insect pollinators in tomato farm is higher than that in chili andstrawberry farm. A good knowledge, good attitude, and bad behaviour are closely related to thedaily air temperature and insect pollinators.
Parameter Air , Produksi dan Pendapatan Tambak Bandeng Sivofishery dan Non-Silvofisheries di Kabupaten Cilacap Nurul Ekawati; Purnama Sukardi; Moh. Husein Sastranegara
Akuatika Indonesia Vol 2, No 1 (2017): Jurnal Akuatika Indonesia (JAkI)
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan Universitas Padjadjaran, Grha. Kandaga (P

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.28 KB) | DOI: 10.24198/jaki.v2i1.23407

Abstract

Permasalahan lingkungan sekarang ini disebabkan oleh kegiatan sosial ekonomi manusia untuk memenuhi kebutuhan hidup. Salah satu cara untuk memenuhi kebutuhan hidupnya, masyarakat pesisir membuka tambak pada hutan mangrove. Pembukaan areal pertambakan pada hutan mangrove menyebabkan fungsi dari hutan mangrove hilang. Pola pertambakan yang dipadukan dengan hutan mangrove (silvofishery) dibuat dengan tujuan untuk kelestarian hutan mangrove dan masyarakat tidak kehilangan mata pencahariannya. Penelitian ini bertujuan untuk mengetahui parameter air, produksi dan pendapatan petambak. Penelitian dilakukan dengan menggunakan metode survai. Pengambilan sampel dilakukan dengan tiga kategori seperti pengambilan sampel air, bandeng, dan masyarakat. Sampel air diambil 2 minggu sekali selama 2 bulan. Sampel bandeng diukur produksinya. Sampel masyarakat diambil melalui wawancara sebanyak 30 orang. Hasil penelitian menunjukkan bahwa kondisi parameter air ke dua tambak layak untuk budidaya ikan bandeng, namun pada tambak non-silvofishery nafsu makan ikan mulai berkurang. Produksi ikan bandeng yang dihasilkan pada tambak silvofishery (66,12 g/m2)lebih tinggi dari tambak non-silvofishery (28,37 g/m2). Pendapatan petambak dari tambak silvofishery (Rp 477.000,-) lebih tinggi dari tambak non-silvofishery (Rp 366.000,-). Secara umum, kondisi parameter air tambak silvofishery dan non-silvofishery yang mempengaruhi produksi dan pendapatan ialah suhu dan TSS. Pada ke dua tambak apabila suhu dan TSS meningkat maka akan meningkatkan produksi dan pendapatan.
Kajian Kualitas Air Pasca-Pengerukan Alur Pelayaran Batu Kapur Sungai Donan Cilacap Yogi Pamungkas; Sidharta Sahirman; Moh. Husein Sastranegara
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.222

Abstract

Donan River dredging impacts on ecological regions and communities around the basin. Dredging from Buoy 27 to Buoy segment 34 as an effort to reduce silting of existing in Cilacap and Donan River shipping channel maintenance efforts limestone PT Holcim Indonesia Tbk. Cilacap Plant. The purpose of the study is to determine the condition of post-dredging water quality in terms of physical and chemical factors, to determine the condition of post-dredging water quality in terms of biological factors; and to analyze the relationship between physical, chemical, and biological factors post-dredging shipping lanes limestone around Cilacap Donan River. The research method used was purposive sampling method. The study was conducted from March-May 2013, with five replications. Samples were taken at three stations namely Station 1 (area before dredging), Station 2 (dredging area), and Station 3 (area after dredging). The results showed that water quality conditions of post-dredging chemical in terms of physical factors tend to decrease at station 2 (TSS amounted of 303.6 mgl-1, BOD5 9.806 mgl-1, COD 171.4 mgl-1), followed by station 3 (TSS 268 mgl-1, BOD5 at 18.182 mgl-1, COD is 262.8 mgl-1) and station 1 (TSS 204.4 mgl-1, BOD5 at 12.258 mgl-1, and COD of 252 , 2 mgl-1). Water quality conditions in terms of post-dredging tend to decrease at station 2 (the number of types makrobenthos by 5 species, number of individuals ind/m2 makrobenthos by 147, the number of types of plankton by 11 species and number of individuals of 1,461 ind/l) followed by station 3 (the number of species makrobenthos by 6 species, number of individuals ind/m2 makrobenthos by 180, the number of types of plankton by 12 species, and the number of individual plankton of 1,341 ind / l), and Station 1 (the number of types makrobenthos by 7 species, number of individuals makrobenthos ind/m2 by 327, the number of types of plankton by 12 species, and the number of individual plankton of 1,340 ind / l). In general, the relationship between physical factors, chemical, and biological post-dredging shipping lanes limestone around the same Cilacapmemilikikecenderungan Donan River bahwakondisi declining water quality at Station 2, followed by Station 3 and Station 1.
Struktur Komunitas Kepiting Intertidal pada Mangrove yang Terdegradasi di Segara Anakan Cilacap Marista Zalindri; Moh. Husein Sastranegara
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.338

Abstract

Makrobenthos intertidal crabs were found living in the sediment and its presence greatly affect the primary productivity in the mangrove ecosystem of Segara Anakan Cilacap. This mangrove area is the largest in the island of Java.  Human activities have caused a lot of pressure. The objective of this study is 1) to determine the structure of the different intertidal crab communities on degraded mangrove; 2) to know the different sediment texture on degraded mangrove in Segara Anakan Cilacap. This research was carried in two mangrove areas based on mangrove canopy cover percentage namely 80% and 30% canopy cover.  The research method for crab community structure was survey conducted by cluster sampling method from January-March 2013. The structure of the intertidal crab of different communities in normal mangrove areas and damaged mangroves. In the normal mangrove area, the number of species found in normal intertidal crab included 15 species consisting of 592 individual, while in the damaged mangrove area there were 14 species consisting of 845 individual. The normal mangrove area has clayey silt sediment texture, while the damaged mangrove area has the sand sediment texture of silt clay.
Kualitas Air Pasca Pengerukan Alur Transportasi Batu Kapur di Sungai Donan Cilacap Mohammad Husein Sastranegara; Sri Lestari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 1 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.1.90

Abstract

Fekunditas Udang Galah (Macrobrachium rosenbergii ) Betina Pasca Ablasi Unilateral dan Suplementasi Vitamin E pada Pakan Rachmawati Nasution; Moh. Husein Sastranegara; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.135

Abstract

This study reports on giant freshwater prawn fecundity with or without unilateral ablation treatment combined with alpha tocopherol (vit E) during 3 month culture. This exsperimental study applied 8 combination treatments of 4 different vit E dosages, i.e. 0, 200, 400, and 600 IU per kg feed with or without unilateral ablation. The results showed that treatments very highly significantly different (p<0,01) in fecundity with the 200 IU vit E dose combined with unilateral ablation was the best (45.228 ± 3.867 egg) optimum resulted in fecundity of the treated females. The overall fecundities averages were ablation group with 0 IU = 41.835 ± 837 eggs; 200 IU = 45.228 ± 3.867 eggs; 400 IU = 43394 ± 1523 eggs ; and the 600 IU = 43.718 ± 2.255 eggs, and for the non ablation with vit E group were 0 IU = 45.293 ± 2.899 eggs, 200 IU = 44.106 ± 1.759 eggs, 400 IU = 46.623 ± 2.556 eggs, and 600 IU=51.824 ± 1.132 eggs. The analysis results also proven that ablation factor gave very highly significantly (p<0,01) and vit E factor separately and in combination with ablation significantly (p<0,05) determined the females fecundity. In conclution, combination of unilateral ablation and tocopherol effective improved fecundity and the combination of unilateral ablation and vit E 200 IU the optimum resulted in giant freshwater prawn females fecundity.
Co-Authors Abdul Aziz Nasihuddin Agus Nuryanto Agus Nuryanto Arumdini, Gustia Atang, Atang Ayunda, Batari Citra Azizah, Rahmatika Annisatul Bhagawati, Dian Budi Aji Dian Baghawati Diana Retna Utarini Suci Rahayu, Diana Retna Utarini Suci Dwi YULIANI Ekawati, Nurul Elly Tuti Winarni Farida Nur Rachmawati Hendro Pramono Imam Aminudin Imam Santosa Kusbiyanto Kusbiyanto Luthfi Hidayat Maulana Marista Zalindri Mujiono Mujiono Mukhtar Effendi Nur Haliza, Fitra Dwi Prodhiana Prodhiana Prodhiana, Prodhiana Pulungsari, Anastasia Endang Purnama Sukardi Rachmawati Nasution Ratna Stia Dewi Shodikin, King Amil Hamzah Al Hakim Sidharta Sahirman Sri Lestari W. Lestari Yogi Pamungkas Yulia Sistina