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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Farmasi Sains dan Komunitas Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Jurnal Atma Inovasia Media Abdimas SENAPAS
P. Kianto Atmodjo
Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Industrial & Manufacturing Engineering Library & Information Science Medicine & Pharmacology Public Health Social Sciences Other

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Pola Pertumbuhan dan Produksi -Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Sisilia Sri Wahyuningsih; Exsyupransia Mursyanti; P. Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2895

Abstract

The aims of this study  were to identify the growth curve of  B. amyloliquefaciens on  corn-starch and non corn starch addition media,  number of cells  and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on both  medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initial  pH of the media ( 5,  5.5,  6,  6.5,  7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production of  a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cells  (about 2.8542 x 108 cells/ml)  and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hours  incubation.
Karakterisasi Sifat-Sifat Fisik dan Mekanik Edible Film Pati Ganyong (Canna edulis Kerr.) Arif Wijoyo; F. Sinung Pranata; P. Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2912

Abstract

This research was carried out in order to reveal ganyong starch’s potential as a major ingredient of edible film, to understand the optimum concentration of ganyong starch and palmitic acid in the making process of edible film and to study their physical and chemical characteristics. The research has two stages, which were the starch preparation, and edible film preparation by using various concentrations of ganyong starch (1, 2, 3, and 4 % w/v) and palmitic acid (1, 2, 3, and 4% w/w). First research stage revealed that ganyong starch has a potential as a major ingredient of edible film because of its high amylose content (32.53% on dry basis). The next stage of the research showed that the optimum ganyong starch concentration of edible film was 2% (w/v). This concentration gives the WVTR (Water Vapor Transmission Rate) measurement of 11.86 (g. mm/m2. hour), film elongation of 2.26%, tensile strength of 3.29 Kpa, and film thickness of 0.0754 mm. The optimum concentration of palmitic acid was 4% (w/v). This results in the WVTR (Water Vapor Transmission Rate) measurement of 7.99 (g. mm/m2. hour), film elongation of 1.24%, tensile strength of 3.58 Kpa, and film thickness of 0.087 mm.
Pelatihan Tentang Pengenalan, Pemeriksaan, dan Penjaminan Mutu Bahan Obat Tradisional (BOT) Bagi Guru Biologi SMA Daerah Istimewa Yogyakarta (Training on Determination, Identification, and Quality Control of Traditional Medicine Ingredients to Biology High School Teachers in Yogyakarta Special Province) Boy Rahardjo Sidharta; Exsyupransia Mursyanti; P. Kianto Atmodjo; Nelsiani To’bungan; Ines Septi Arsiningtyas
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 2, No 2 (2018): Vol 2, No 2 (2018)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.149 KB)

Abstract

AbstrakKegiatan Pengabdian kepada Masyarakat dengan topik “Pelatihan tentang Pengenalan, Pemeriksaan, dan Penjaminan Mutu Bahan Obat Tradisional (BOT) bagi Guru Biologi SMA Daerah Istimewa Yogyakarta” dilakukan mengingat banyaknya kasus keracunan akibat konsumsi obat tradisional. Guru Biologi SMA menjadi sasaran pelatihan karena telah memiliki latar belakang keilmuan dan keterampilan yang memadai serta sering menghadapi pertanyaan orangtua murid dan masyarakat perihal pemanfaatan BOT. Pelatihan diharapkan memberikan solusi terhadap permasalahan di atas dengan menerapkan teknologi tepat guna yang sederhana, sehingga dapat memberikan pemahaman tentang BOT berkualitas berdasarkan Cara Pembuatan Obat Tradisional yang Baik (CPOTB). BOT yang dipilih yaitu sambiloto (Andrographis paniculata), mahkota dewa (Phaleria macrocarpa), dan pule (Alstonia scholaris) karena banyak digunakan dalam pengobatan diabetes mellitus. Peserta diberikan keterampilan mengenali, mengidentifikasi, dan melakukan pemeriksaan mutu secara sederhana, namun ilmiah. Peserta menyatakan mampu menerapkan keterampilan yang diperoleh dan bersedia mengikuti pelatihan lanjutan di masa mendatang. Kata kunci: Pengenalan, Pemeriksaan, Penjaminan Mutu, Bahan Obat Tradisional  AbstractCommunity Service Activity with the topic “Training on Determination, Identification, and Quality Control of Traditional Medicine Ingredients to Biology High School Teachers in Yogyakarta Special Province” was done due to the increase of traditional medicine intoxications. Biology High School teachers were targeted as the participants, because they had scientific background and skills related to the problem. The activity was done to give better solution to the problem using simple and appropriate technology, hence it can give knowledge on high quality of traditional medicine based on Good Production of Traditional Medicine. Traditional medicines utilised were sambiloto (Andrographis paniculata), mahkota dewa (Phaleria macrocarpa), and pule (Alstonia scholaris), since these traditional medicines were mostly practiced to cure diabetes. Participants were also given skills on determination, identification, and quality control. Participants stated that they were able to apply the skills obtained and were ready to be included in the continual training in the future. Key words: Determination, Identification, Quality Control, Traditional Medicine
Efek Pemberian Sari Buah Berenuk (Crescentia cujete L.) Terhadap Berat Mencit Galur Swiss-Webster (Mus musculus) Dhany Krisna; Patricius Kianto Atmodjo; Ines Septi Arsiningtyas
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 7, No 2 (2022): June 2022
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v7i2.5255

Abstract

Buah berenuk (Crescentia cujete L.) merupakan salah satu tanaman yang digunakan untuk pengobatan tradisional. Buah berenuk mengandung berbagai senyawa seperti flavonoid, yang dapat digunakan untuk meningkatkan nafsu makan. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan sari dari buah berenuk (Crescentia cujete L.) dalam meningkatkan nafsu makan dari mencit (Mus musculus) galur Swiss Webster, yang diukur berdasarkan tingkat konsumsi pakan dan berat mencit. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan yaitu lima dosis sari buah berenuk 20%, 40%, 60%, 80%, dan 100%, satu kontrol negatif tanpa pemberian sari buah berenuk, serta satu kontrol positif menggunakan perlakuan sari temulawak (Curcuma xantorrhiza). Hasil penelitian menunjukkan bahwa sari buah berenuk mengandung senyawa fenolik dan flavonoid. Sari buah berenuk dosis 40% mampu meningkatkan konsumsi pakan serta berat badan mencit meskipun secara analisis statistik tidak berbeda signifikan dengan dosis lain. Dari data tersebut dapat disimpulkan bahwa sari buah berenuk memiliki potensi untuk meningkatkan nafsu makan. 
POTENSI ANTIMIKROBIA KRIM EKSTRAK RANTING PATAH TULANG (Euphorbia tirucalli Linn.) TERHADAP Propionibacterium acnes ATCC 11827 DAN Candida albicans ATCC 24433 Melina Scandinovita Setiorini; C.J. Soegihardjo; Kianto Atmodjo
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 11, No 2 (2014)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.307 KB) | DOI: 10.24071/jpsc.00102

Abstract

Abstract: Euphorbia tirucalli L. (Euphorbiaceae) or patah tulang has been used traditionally in Java fortreatment of fungal infections (Propionibacterium acnes causing acne and Candida albicans causingcandidasis). Information on their use is available, but scientific data on their bioactivity and safety ofactions still scanty. A study was conducted on the effect of organic extracts of this plant on fungal strains.Aceton extract were evaluated through the disc diffusion assay. Aceton extract was prepared by Soxhletapparatus for eight hours. Bacteria and yeast test strains were cultured on TSA (Trypthone Soya Agar)and on PDA (Potato Dextrose Agar) for Candida albicans. A 0.5 McFarland standard suspension wasprepared. Sterile paper discs 6 mm in diameter impregnated with 10 ml of the test extract (100 mg/ml)were aseptically placed onto the surface of the inoculated media. Thymol 0,5% and ketokonazol 2% wereused as standards. Discs impregnated with dissolution medium were used as controls. Activity of theextracts was expressed according to zone of inhibition diameter. Dimethylsulfoxide (DMSO) was used forpreparing test extracts (10, 20, 40, 60, 80, 100%) and were tested for Minimum Inhibitory Concentration(MIC) to Propionibacterium acnes and Candida albicans. MIC were obtained that 100% test extract wasgreatest and 10% test extract was weakest. Then, test extracts were prepared by concentrations 10, 9, 8, 7,6, 5%, and the result were the best were 10% test extract to Propionibacterium acnes and 6% test extractto Candida albicans. Antimicrobial test for pharmaceutical preparation, i.e. cream (o/w) were preparedusing 9 and 10% test extracts mixed with cream for testing to Propionibacterium acnes and 5 and 6% testextracts with cream for testing Candida albicans.The final results were 10% test extract had MIC toPropionibacterium acnes and 6% test extract had MIC in cream to Candida albicans.Keywords: Euphorbia tirucalli L., DMSO, Thymol, antimicrobial potency, cream (o/w), Propionibacteriumacnes, Candida albicans
Pengolahan Sirup dan Selai Markisa oleh Kelompok Tani Kampung Markisa untuk Memasuki Era Pascapandemi Benediktus Yudo Leksono; Patricius Kianto Atmodjo
Media Abdimas Vol 1 No 3 (2022): Jurnal Media Abdimas Vol 1 No 3 Bulan November 2022
Publisher : Universitas Persada Indonesia YAI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.857 KB) | DOI: 10.37817/mediaabdimas.v1i3.2558

Abstract

Kelompok Tani Markisa Kampung Markisa Blunyahrejo telah memiliki kemampuan untuk mengolahmarkisa menjadi produk olahan berupa sirup markisa dan selai markisa. Meski demikian, adanya pandemimengakibatkan ketidakaktifan produksi dari sirup dan selai markisa. Oleh karena itu, Kelompok TaniMarkisa Kampung Markisa memerlukan kegiatan pelatihan kembali sebagai bentuk penyegaran danmemotivasi kembali anggota kelompok tani untuk kembali mengolah markisa. Kegiatan pengabdian inibertujuan untuk memotivasi dan menyegarkan kembali Kelompok Tani Markisa, Kampung Markisa,Blunyahrejo, dalam mengolah markisa menjadi produk sirup dan selai markisa. Kegiatan pengabdian terdiridari diskusi dan survey kecil serta kegiatan pelatihan dan praktek bersama. Sirup yang dihasilkan memilikikekentalan menyerupai sirup komersial, sedangkan selai memiliki warna cerah dengan kenampakanmenarik. Setelah mengikuti kegiatan pengabdian, Kelompok Tani Markisa Kampung Markisa, Blunyahrejokembali termotivasi dan memiliki kemampuan untuk mengolah markisa menjadi produk olahan yangmemiliki kualitas lebih baik dibandingkan dengan produk yang telah dibuat sebelumnya.
The Secondary Metabolite and Antibiotic Activity of Calabash Leaf and Fruit (Crescentia Cujete L) Ethanol Extract Kianto Atmodjo; Boy Rahardja Sidharta
Seminar Nasional Penelitian dan Abdimas Vol 1 No 1 (2023): Juni
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/senapas.v1i1.7395

Abstract

Antibiotic resistance is a big problem in medication, food security, and human development goals. Indonesia has a very huge diversity in term of medicinal plants, which are proven to cure many illnesses by their ancestors, including Calabash. However, research on the bioactive substances from the leaves and fruit of calabash as antibiotic [both antibacterial and antifungal] are dearth. Antibiotic activity test of the substances found has never been done yet, including the comparison to antibiotic ampicillin. The objectives of the research are: a] to determine bioactive substances found in the leaves and fruit of Calabash which have antibiotic activities, b] to reveal the antibiotic activity of leaves and fruit extract of calabash to two [2]] pathogenic bacteria and two [2] pathogenic fungus, c] to determine minimum inhibitory concentration [MIC] of calabash’s leaves and fruit extract to four [4] pathogenic microbes. The methods applied in this research are as follows. The bioactive substances were extracted using maceration method with ethanol as the solvent. The extracts were then analyzed using GC-MS equipment and tested to four [4] pathogenic microbes in agar diffusion method to determine the inhibition zones of the extracts. Later, the extract was also used to determine the MIC of the extracts by dilution technique. This present research found that fruit and leaves extract have many bioactive substances such as saponin, flavonoid, terpenoid and alkaloid that have low-medium antibacterial and antifungal activity, with minimum concentration of inhibition 50% for bacteria and 75% for fungus., which later can be developed into products or antibacterial and antifungal drugs that may give benefits to Indonesian people and the world.
Pelatihan dan Pendampingan Prapanen, Panen dan Pascapanen kepada Petani Jahe Area Paroki Boro, Kabupaten Kulon Progo junedi, sendy; Ines Septi Arsiningtyas; P Kianto Atmodjo; Exsyupransia Mursyanti; Boy Rahardjo Sidharta; Stefani Santi Widhiastuti
Jurnal Atma Inovasia Vol. 3 No. 5 (2023)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v3i5.7594

Abstract

Paroki Santa Theresia Lisieux (STL) Boro terletak pada area pengembangan pariwisata sehingga berpotensi menjadi desa wisata dengan amenitas khas berupa jahe emprit. Produksi jahe pada area ini memiliki keterbatasan kuantitas, kontinuitas, dan kualitas karena minimnya pengetahuan dan ketrampilan petani dalam penanaman dan pemeliharaan tanaman serta pengolahan rimpang jahe menjadi produk jadi. Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta memberikan pelatihan dan pendampingan untuk memperbaiki pengetahuan dan ketrampilan petani jahe di area Paroki STL Boro. Pelatihan dan pendampingan dibagi menjadi tujuh kali pertemuan yang terdiri dari sosialisasi dan evaluasi pengetahuan dalam bentuk kuesioner, dilanjutkan dengan pelatihan tentang prapanen, panen, pascapanen primer dan sekunder, serta yang terakhir adalah evaluasi berupa diskusi dan kuesioner. Topik pertemuan pascapanen sekunder terdiri dari pembuatan produk kesehatan (serbuk jahe instan) dan produk kosmetik (lulur jahe). Hasil analisis data kuesioner secara semikuantitatif menunjukkan terjadi peningkatan pengetahuan petani sebesar 75% antara sebelum dan sesudah pelatihan. Dengan adanya pelatihan, petani di Paroki STL Boro mampu menghasilkan produk jahe instan yang telah dipasarkan secara terbatas.
Daya Antibakteri Minyak Atsiri Daun Sirih Hijau (Piper betle L.) dan Sirih Merah (Piper Crocatum) Terhadap Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus Lidwina Ella Septiani; Atmodjo, Kianto; B. Boy Rahardjo Sidharta
Seminar Nasional Penelitian dan Abdimas Vol 2 No 1 (2024): Juni
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

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Abstract

Abstract —Betel, have long been known as antibacterial. Based on the shape of the leaves, taste and aroma, betel is divided into several types, but the most widely used are green and red betel leaves. Green and red betel leaves contain phytochemicals such as essential oils compounds such as kavikol, cineol and eugenol. The purpose of this study was to determine the differences in the content of green and red betel leaf essential oil in the old and young treatments and its effectiveness at the concentration used against Staphylococcus aureus and Pseudomonas aeruginosa bacteria. This research begins with the preparation of betel leaf for essential oil distillation, isolation of essential oils by steam-water distillation, analysis of components or compounds of essential oils using GCMS, preparation of pure essential oil stock solutions (100%), preparation of Staphylococcus aureus and Pseudomonas aeruginosa bacteria. then identification test was carried out with several test methods, antibacterial activity testing which began with making variations in concentrations of 10%, 15%, 20%, 25% and 30% with the addition of a positive control of ampicillin disk and a negative control of DMSO with the well diffusion method which was incubated for 24 hours. hours at 37°C. The results of the measurement of the inhibitory zone were analyzed by ANOVA using the factorial RAL pattern, followed by the measurement of the minimum inhibitory concentration (MIC) with a concentration variation of 5%, 10%, 20% and 30% and the determination of the minimum inhibitory concentration (MIC). The results showed that dark green betel leaf essential oil had better antibacterial activity in the inhibition zone test with a concentration of 30% against both bacteria with an inhibitory zone area of ​​1.88 cm for Staphylococcus aureus and 1.21 cm for Psedumonas aeruginosa and the minimum inhibitory concentration test was able to inhibit at a concentration of 10% 10% against Staphylococcus aureus bacteria and at a concentration of 5% on Psedumonas aeruginosa bacteria.
Co-Authors Abda Abda Aida, Yuniarti Arif Wijoyo B. Boy Rahardjo Sidharta B. R. Sidharta Boy R. Sidharta Boy Rahardja Sidharta Boy Rahardjo Sidharta Boy Rahardjo Sidharta C.J. Soegihardjo Dhany Krisna E. Mursyanti Exsyupransia Mursyanti Exsyupransia Mursyanti H. N. Lestari Ines Septi Arsiningtyas Ines Septi Arsiningtyas Laudensius, Oktaf Leksono, Benediktus Yudo Lidwina Ella Septiani Melina Scandinovita Setiorini Mursyanti, Mursyanti Pramana Yuda Sendy Junedi Sisilia Sri Wahyuningsih To'bungan, Nelsiani Widhiastuti, Stefani Santi