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All Journal Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Aplikasi Teknologi Pangan Jurnal Kimia Terapan Indonesia JURNAL PANGAN
Aspiyanto Aspiyanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Engineering Immunology & microbiology Materials Science & Nanotechnology Social Sciences Other

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SISTEM MODUL MIKROFILTRASI DALAM PEMURNIAN L-THEANINE DARI TEH HIJAU LOKAL (Camellia assamica) Pekoe UNTUK MINUMAN FUNGSIONAL Agustine Susilowati; Aspiyanto Aspiyanto; Hakiki Melanie; Yati Maryati
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2010): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 1 2010
Publisher : Prosiding SNST Fakultas Teknik

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Abstract

Microfiltration (MF) module system had a potential use in purifying of L-Theanine of local green tea (Camellia assamica) grade Pekoe for functional drink as anti stress compound. Purification process was performed through MF membrane of 0.2 µm at pump motor frequency of 20 Hz, room temperature, and operation pressure of 4 and 6 bar for 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300 minutes. The experiment result showed that MF system was able to pass more much L-Theanine in permeate, but retained more much dry matter and dissolved protein in retentate. Long purification time would decrease permeate flux value and L-Theanine concentration in permeate, but increased dry matter and dissolved protein in retentate. Optimum operation condition based on the highest concentration of L-Theanine in permeate at operation pressures of 4 and 6 bar for 120 and 240 minutes was able to pass L-Theanine in permeate of 86.33 % and 71.21 from all L-Theanine prior to process. Permeate as source of L-Theanine at optimum condition gave permeate flux values of 13.72 and 15.55 L/m2.hour with concentrations of L-Theanine of 10.721 % and 10.203 % (dry weight), dissolved protein of 2.1 and 1.8 mg/mL, and   dry matter of 1.342 and 1.712 %. Retentate has a potential use as functional compounds for anti oxidant,  anti cancer, anti diabetes and anti cholesterol. Key Words : Camellia  asssamica, L-Theanine, Microfiltration (MF), permeate, retentate.
SISTEM CROSS FLOW DALAM PEMISAHAN SENYAWA FLAVOR SERUPA DAGING (Meatlike flavor) DARI KACANG HIJAU (Phaseolus radiatus) TERFERMENTASI OLEH Rhizopus oligosporus MELALUI MIKROFILTRASI Agustine Susilowati; Aspiyanto Aspiyanto
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2010): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 1 2010
Publisher : Prosiding SNST Fakultas Teknik

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Abstract

Cross-flow Microfiltration System is one of important separation basic using membrane influenced by separation process condition (pressure, flow rate, temperature, time), particles size and molecular weight (MW). The goal of this experiment was to find out composition, type and intensity of flavor compounds produced from autolysate separation of meatlike flavor from fermented mung bean (Phaseolus radiatus L.) through MF membrane at pump motor frequency 20 Hz, room temperature and operation pressure 6 bar for 90 minutes. The experiment results showed that Cross-flow MF System at permeate flux value of 31.83 L/m2.hour was able to separate N-Amino and dissolved protein as meatlike flavor compounds pass through MF membrane more much in permeate, while total solid, fat salt and  protein were rejected and retained more much in concentrate/retentate. Meatlike flavor compounds were dominated by sulphur. MF system was able to separate more much sulphur compounds as meatlike flavor present in permeate than concentrate/retentate. On the permeate was obtained 43 compound of meat analogue dominated by Sulphur-Nitrogen component (71.48%), namely 1,2,3-Triazole,4-flourodinitromethyl-1-methyl (0,01%), 2–Thiopenethiol (0,1%), Chlomethiazole (0,22%), 4-Methyl-5-hydroxyethylthiazole (70,99%), Furfuril-methyl-sulfide (0,11%), 2-Methyl-6-thiopurine (0,05% Area/0,2µg sample), While, other components covered Nitrogen (11), Pyran (4), Furan (5) , Alcohol  (4), Aldehiyde (3), Hidrocarbon (1) and  Ester - organi acid (5).Permeate has potential use as meatlike flavor extract, whereas retentate as concentrate/Hydrolyzed Vegetable Protein (HVP) is used for vegetable food products with meat taste and aroma. Key Words : Meat analog flavor, flavoring reaction, permeate, concentrate/retentate, microfiltration (MF).
Pusat Penelitian Kimia LIPI, Kawasan Puspiptek, Serpong Agustine Susilowati; Yetti M. Iskandar; Aspiyanto Aspiyanto; Yaty Maryati
JURNAL PANGAN Vol. 20 No. 4 (2011): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v20i4.182

Abstract

Rhizopus C1 adalah kultur dari Rhizopus oligosporus yang diisolasi dari usar tempe Malang dan biasa digunakan sebagai inokulum tempe. Potensinya sebagai sumber enzim protease dapat dimanfaatkan dalam menghidrolisis protein sorgum (Sorgum bicolor L Moench) untuk memperoleh serat pangan (Dietary Fiber) yang berperan sebagai anti kolesterol dan anti konstipasi. Perlu diketahui bahwa serat pangan tidak termasuk protein, sehingga hidrolisis protein diharapkan dapat membentuk asam-asam amino dengan berat molekul rendah dan tidak berpengaruh terhadap Total Serat (TDF/Total Dietary Fiber). Hidrolisis dilakukan pada suhu 60°C, pH 5,5 dengan variasi waktu hidrolisis yaitu 30 menit (W 30’) dan 60 menit (W 60’) dan variasi konsentrasi Rhizopus C1 dalam substrat sorgum mulai dari nol (kontrol) sampai 0,8 persen (b/b protein) pada liquisat yaitu hasil hidrolisis sorgum dengan a-Amylase. Hasil penelitian memperlihatkan bahwa hidrolisis dengan konsentrasi Rhizopus C1 dan waktu proses hidrolisis yang berbeda berpengaruh terhadap aktifitas proteolitik dan amylolitik Rhizopus C1, total padatan, N-Amino, TDF, protein terlarut dan gula pereduksi hidrolisat. Kondisi optimum hidrolisis berdasarkan TDF tertinggi diperoleh pada konsentrasi Rhizopus C1 0,6 persen (b/b) selama 30 menit (W 30’) dengan peningkatan TDF sebesar 63,021 persen dibandingkan kontrol tanpa penambahan Rhizopus C1 (0 persen).Rhizopus C1 is a culture of the Rhizopus ologosporus isolated from Malang tempe usar and used in fermentation of tempe. Its potential as the source of protease enzyme can be used to hydrolyze sorgum (Sorghum bicolor L. Moench) protein in order to get Dietary Fiber (DF) as the functional food for anti cholesterol and anti constipation.DF does not include protein, so that hydrolysis of protein can be expected to degrade and convert amina groups into amino acids with low molecular weight (MW). Therefore it does not affect the Total Dietary Fiber (TDF). Hydrolysis process was carried out at the concentration of Rhizopus C1 in sorghum substrate varied from zero (control) to 0.8 percent (w/w) onto the liquifisate (sorghum hydrolysate with a-Amylase), with pH of 4.5 and 5.5 and temperature of 60°C for the duration of 30 and 60 minutes.The result demonstrated that the variation of process conditions (Rhizopus C1 concentration and time) affected the proteolytic and amylolytic activities of Rhizopus C1, total solids and reduction of dissolved protein, and the increase of TDF of liquifisate. Based on the highest TDF yield, the optimal condition of protease hydrolysis was reached at Rhizopus C1 concentration 0.6 percent (w/w) for 30 minutes. 
Pemisahan Eksopolisakarida (Eps) Sebagai Metabolit Bakteri Usus untuk Aditif Makanan dalam Biomassa Pati Sagu {Metroxylon sp.) dan Glukosa melalui Sistem Mikrofiltrasi Sel Berpengaduk (Separation of Exopolysaccharides (Eps) As Colon Bacteria Metabolismfor FoodAdditive in Sago Starch Biomass (Metroxylon sp.) and Glucose through Membrane Cell Microfiltration System) Agustine Susilowati; Aspiyanto Aspiyanto; Achmad Dinoto; Puspa D. Lotulunga
JURNAL PANGAN Vol. 21 No. 4 (2012): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v21i4.203

Abstract

Kultur bakteri usus Lactobacillus sp. FU-0811 dan Enterobacter sp. FU-0813 yang ditumbuhkan pada medium berupa biomassa pati sagu (Metroxylon sp.) menghasilkan eksopolisakarida (EPS) yang berpotensi sebagai aditif makanan (pengental, pengemulsi, penstabil, pembawa). Penggunaan pati sagu merupakan alternatif biomassa selain glukosa. Melalui pemisahan dengan sistem membran mikrofiltrasi (MF) 0,15 pm berpengaduk diharapkan EPS dan metabolit lainnya diperoleh dengan konsentrasi lebih optimal. Penelitian ini bertujuan untuk mengetahui pengaruh pemisahan EPS hasil pertumbuhan bakteri usus Lactobacillus sp. FU-0811 dan Enterobacter sp. FU-0813 masing-masing dalam media biomassa pati sagu dan sebagai pembanding digunakan biomassa glukosa pada kondisi proses pemurnian tetap (kecepatan putar sel pengaduk 400 rpm, suhu ruang dan tekanan proses 40 psia) terhadap metabolit dengan komposisi terbaiksebagai bahan food aditif. Hasil penelitian menunjukkan bahwajenis biomassa, jenis mikroba dan sistem membran MF berpengaruh terhadap tingkat pemisahan metabolit. Retentat atau konsentrat hasil pemisahan memiliki komposisi dan jumlah mikroba lebih baik daripada permeat atau ekstrak untuk kedua jenis bahan tersebut. Proses membran MF terhadap biomassa glukosa dengan Lactobacillus sp. FU-0811 dan Enterobacter sp. FU-0813 masing-masing mampu menahan EPS sebagai gula reduksi dalam retentat atau konsentrat berturut-turut 73,73 persen dan 47,33 persen, serta pada biomassa pati sagu berturut-turut 95,5 persen dan 83,435 persen apabila dibandingkan dengan total gula reduksi dalam permeat dan retentat atau konsentrat pada masing-masing biomassa. Hasil idensifikasi metabolit melalui LC-MS memperlihatkan bahwa intensitas senyawa monosakarida dalam retentat atau konsentrat lebih tinggi daripada yang terdapat di dalam permeat.Colon bacteria culture of Lactobacillus sp. FU-0811 and Enterobacter sp. FU-0813 grown on biomass ofsago (Metroxylon sp.) produced exopolysaccharides (EPS) that have an important potential useas food additive (thickener, emulsifier, stabilizer and carrier). The useofsago starch isan alternative biomass beside glucose. By applying the stirred microfiltration membrane (0.15 pm ofpore size) cell, the biomass was separated to get EPS and other metabolites with more optimal concentration. The goal of this experiment was to find out separation effect ofEPS as a result of the growth ofcolon bacteria of Lactobacillus sp. FU-0811 and Enterobacter sp. FU-0813 in the biomass ofsagostarch. Asa comparison, glucose was used on fixed condition ofpurification process (rotation speed ofstirrer cell of400 rpm, room temperature and pressure of 40 psia) and the best composition of metabolite as food additive agent. The result showed that the type biomass, microbe, and MF membrane system influenced on the level of metabolite separation. The retentate or the concentrate of separation had better composition and microbial count than that of the permeate orextract for both biomasses. The process of MF membrane on glucose biomass with Lactobacillus sp. FU-0811 and Enterobacter sp. FU-0813 were subsequently able toretain EPSas reducing sugar in the retentate orconcentrate by 73.73 percent and 47.33percent, and the biomass ofsago starch by95.5percent and 83.435 percent when compared to total ofreducing sugar in permeate and retentate or concentrate for each biomass. The result ofmetabolite identification through LC-MS instrument displayed that greater intensity of monosaccharide compound was found in the retentate or concentrate than that of in the permeate. 
PEMEKATAN HIDROLISAT DEDAK SORGUM (Sorghum bicolor L Moench) B-100 HASIL HIDROLISIS KULTUR Rhizopus C1 MELALUI ULTRAFILTRASI SEL BERPENGADUK UNTUK ANTI KOLESTEROL Agustine Susilowati; Aspiyanto Aspiyanto; Y M Iskandar
Jurnal Aplikasi Teknologi Pangan Vol 1, No 3 (2012): Agustus 2012
Publisher : Indonesian Food Technologists

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Abstract

Telah dilakukan pemekatan hidrolisat dedak sorgum (Sorghum bicolor L Moench) B-100 hasil hidrolisis kultur Rhizopus C1,melalui ultrafiltrasi (UF) sel berpengaduk untuk memperoleh SDF/soluble dietary fiber sebagai anti kolesterol. Ultrafitrasi dilakukan pada tekanan 30 dan 40 psia dengan kecepatan putar pengaduk yaitu 200, 300 dan 400 rpm. Hasil penelitian menunjukkan bahwa kondisi operasi pemekatan berpengaruh terhadap fluks permeat dan komposisi retentat & permeat. Perlakuan optimal berdasarkan SDF tertinggi dicapai pada tekanan 40 psia dengan kecepatan putar 300 rpm yang menghasilkan konsentrat dengan komposisi SDF 2,1575% (b.k), total padatan 1,8017 %, protein terlarut 0,0492 mg/mL, total protein 1,432 (b.k), tanin  0,043 % dan gula reduksi 1,744 mg/mL pada fluks 1,5 mL/cm2. menit.
Perbedaan Karateristik Oligofruktosa dan Komposisi Serat Inulin Hasil Hidrolisis dan tanpa Hidrolisis Enzim Inulinase Acremonium Sp-Cbs3 dari Umbi Dahlia Merah (Dahlia sp. L) Lokal untuk Anti Kolesterol Agustine Susilowati; Puspa D Lotulung; Yetti Mulyati Iskandar; Aspiyanto Aspiyanto
Jurnal Aplikasi Teknologi Pangan Vol 4, No 4 (2015): November 2015
Publisher : Indonesian Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.782 KB) | DOI: 10.17728/jatp.v4i4.5

Abstract

Fruktooligosakarida (FOS) yang dihasilkan oleh umbi dahlia merah (Dahlia sp. L) Sukabumi melalui ekstraksi secara kimia maupun hidrolisis enzimatik berpotensi sebagai serat larut air (SDF) untuk anti kolesterol. Ekstraksi inulin secara kimia dihasilkan inulin A, sedangkan hidrolisis enzimatik dilakukan terhadap inulin A menggunakan enzim inulinase kasar kapang Acremonium sp-CBS3 dihasilkan hidrolisat inulin B. Karakterisasi dilakukan terhadap komposisi kimia dan idensifikasi oligomer menggunakan LCMS. Hasil penelitian menunjukkan perbedaan komposisi dan karateristik oligomer kedua bahan tersebut. Berdasarkan intensitas dan jumlah keluaran peak pada kromatogram diketahui bahwa hidrolisat inulin B lebih banyak menghasilkan peak (5) dibandingkan dengan inulin tanpa hidrolisis(A) (1 peak). Pada keadaan ini hidrolisat inulin menghasilkan oligomer dengan intensitas 100% berturut-turut sebagai T 1,9, T 2,4, T 2,6, T 3,1 danT 3,4 yang di dominasi oligomer berberat molekul berturut-turut sebesar 186, 202, 174, 174 dan 174 Da dengan kata lain oligomer berberat molekul 174 Da lebih mendominasi dibandingkan oligomer yang lain, sedangkan gel inulin didominasi oligomer berberat molekul lebih tinggi (181 Da) yang ditunjukkan pada mass spektra dari T 3,3. Hal ini menunjukkan bahwa  perolehan SDF melalui hidrolisis menggunakan kapang Acremonium sp-CBS3 menghasilkan oligomer dengan berat molekul rata-rata yang lebih rendah sehingga dimungkinkan lebih tinggi sifat fungsionalnya sebagai anti kolesterol
PENERAPAN MEMBRAN MIKROFILTRASI PADA PEMURNIAN EKSTRAK KALDU KACANG HIJAU (Phaseolus radiatus L.) SEBAGAI FORTIFIKAN PRODUK MAKANAN Aspiyanto Aspiyanto; Agustine Susilowati
Jurnal Kimia Terapan Indonesia Vol 12, No 2 (2010)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5605.66 KB) | DOI: 10.14203/jkti.v12i2.216

Abstract

In preparation of mung bean (Phaseolus radiatus L.) broth extract asfortificant agent offood products via purification by means of microfiltration (MF) membrane, operation conditions have important role. Application of MF membrane modul is afurther process of purification from small scale (Stirred MF cell, 30.175 cm2 of membrane area) to bench-scale (Cross-flow MF modul, 360 cm2 of membrane area). The main goal of this work was to find out effect of flow rate of material and operation pressure on compositions of concentrate/retentate and permeate from mung bean broth extract as savoury (umami)-based fortificant agent offood products. Preparation of mung bean broth extract was conducted by introducing broth extract of crude mung bean to Cross-flow MF modul at pump motor frequency of l0 and 20 Hz, room temperature and operation pressure of 2, 4 and 6 bar, respectively. Investigation was performed on permeate flux value, and analysis was conducted on concentrate/retentate and permeate, covering on total solids, dissolved solid, total protein, dissolved protein, N-amino and salt. The result of experiment showed that pump motor frequency and operation pressure affect on concentrate/retentate and permeate compositions. Increase of pump motor frequency and operation pressure increase permeate composition, but they tend to be almost similar on concentrate/retentate of mung bean broth extract. Treatment combination at pump motor frequency of 20 Hz and operation pressure of 2 bar generated the best composition of concentrate/retentate as hydrolyzed vegetable protein (HVP) with concentrations of total solids of 3.98 %, soluble solid of 6.05 %, total protein of 5.65 %, dissolved protein of 5.3 mg/mL, N-amino of 0.95 mg/mL and salt of 2.04 %, while permeate gave flux value of 32.19 L/m2.hour with concentrations of total solids of 5.74 %, soluble solid of 5.04 %, total protein of 4.51 %, dissolved protein of 4.05 mg/mL, N-amino ofl.08 mg/mL and salt of 2.2 %, and had useful potency asfood savoury flavor.Keyword : Mung bean (Phaseolus radiatus L.) broth, microfiltration (MF), concenirate/reieniaie, permeate, hydrolysate.
SEPARATION OF SAVORY FRACTION FROM AUTOLYSATE OF KIDNEY BEAN (Phaseolus vulgaris L.) FERMENTED BY Rhizopus sp-PL19 THROUGH CROSS-FLOW MICROFILTRATION (CFMF) MEMBRANE MODULE Aspiyanto Aspiyanto; Agustine Susilowati; Yati Maryati
Jurnal Kimia Terapan Indonesia Vol 16, No 1 (2014)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2613.123 KB) | DOI: 10.14203/jkti.v16i1.7

Abstract

Separation of savory fraction from autolysate of kidney bean (Phaseolus vulgaris L.) fermented by Rhizopus sp.-PL19 as potential seasoning agent was performed using cross-flow microfiltration (CFMF) technique in module scale. The goal of this experiment was to find out the effect of pressure and time on performance of MF membrane, and compositions of retentate and permeate. The result of experiment showed that separation and/or purification using CFMF technique gave permeate as savory fraction with better clearness and sharper aroma than retentate, but retentate had better composition than permeate. During separation increased total protein, dissolved protein and salt concentrations, but decreased total solid, reducing sugar and fat concentrations in permeate at pressure of 2 and 4 bar. Based on N-Amino concentration in permeate, the optimum condition of separation was reached at pump motor frequency of 10 Hz and room temperature with  pressure of 2 and 4 bar for 10 minutes. This condition produced permeate with fluxes of 51.11 and 52.55 L/m2.hour, and concentrations of N-Amino of 5.50 and 9.80 mg/mL, dissolved protein of 1.01 and 0.97 mg/mL, total protein of 4.85 and 12.10 % (dry weight), reducing sugar of 55.75 and 53.75 mg/mL, salt of 1.16 and 1.06 %, fat of 0.41 and 0.65 %, and total solids of 7.82 and 6.35 %, respectively. Keywords : Autolysate, kidney bean (Phaseolus vulgaris L.), Rhizopus sp.-PL19, cross-flow microfiltration (CFMF), permeate, retentate.  Pemisahan fraksi gurih dari autolisat kacang merah (Phaseolus vulgaris L.) terfermentasi oleh Rhizopus sp.-PL19 sebagai bahan seasoning potensial dilakukan menggunakan teknik mikrofiltrasi cross-flow (MFCF) pada modul.Penelitian bertujuan untuk mencari pengaruh tekanan dan waktu pemisahan terhadap kinerja membran mikrofiltrasi (MF) dan komposisi dalam retentat dan permeat. Hasil penelitian menunjukan bahwa pemisahan dan/atau pemurnian menggunakan teknik MFCF memberikan permeat sebagai fraksi gurih savory dengan kejernihan lebih baik dan aroma lebih tajam daripada retentat, tetapi retentat memiliki komposisi lebih baik daripada permeat. Pemisahan dengan waktu lama menaikkan konsentrasi total protein, protein terlarut dan garam, namun mengurangi konsentrasi total padatan, gula pereduksi dan lemak dalam permeat pada tekanan 2 dan 4 bar. Berdasarkan konsentrasi N-Amino dalam permeat, kondisi optimum pada pemisahan dicapai dibawah frekuensi motor pompa 10 Hz dan suhu ruang dengan tekanan 2 dan 4 bar selama 10 menit. Kondisi ini menghasilkan permeat dengan fluks masing-masing 51,11 dan 52,55 L/m2.jam serta konsentrasi N-Amino of 5,50 dan 9,80 mg/mL, protein terlarut 1,01 dan 0,97 mg/mL, total protein  4,85 dan 12,10 % (berat kering), gula pereduksi 55,75 dan 53,75 mg/mL, garam 1,16 dan 1,06 %, lemak 0,41 dan 0,65 % serta total padatan 7,82 dan 6,35 %. Kata kunci : Autolisat, kacang merah (Phaseolus vulgaris L.), Rhizopus sp.-PL19, mikrofiltrasi cross-flow (CFMF), permeat, retentat.
Co-Authors Achmad Dinoto Agustine Susilowati Agustine SUSILOWATI Agustine Susilowati Agustine Susilowati Hakiki Melanie Puspa D Lotulung Puspa D. Lotulunga Y M Iskandar Yati Maryati Yaty Maryati Yetti M. Iskandar Yetti Mulyati Iskandar