Rantan Krisnan
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Impact of In Ovo Adenosine Triphosphate (ATP) Injection on Digestive and Antibody-Related Organ Development and Health in Post-Hatch Sentul Chickens Yudiputra , Muhammad Ilham; Ulfah, Maria; Krisnan, Rantan
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 2 (2025): August 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.02.14

Abstract

The Sentul chicken is a native Indonesian breed known for its good genetic potential as a broiler chickens but often has suboptimal growth and feed efficiency. In Ovo feeding (IOF), the administration of nutrients directly into the egg during incubation offers an alternative strategy to enhance embryonic development. This study aimed to evaluated the effects of ATP injection via IOF on the development of digestive and immune organs, as well as the health status of post hatching Sentul chickens. A commercial ATP mixture containing ATP, vitamin B12, Mg-aspartate, K-aspartate, and sodium selenite was injected into 200 eggs on day 18 of incubation. Observations included those of digestive organs (duodenum, jejunum, ileum, cecum, and proventriculus), animal-related organs (bursa of the fundus and spleen), and blood profiles (erythrocytes, leukocytes, and leukocyte differentiation). The results revealed that ATP injection significantly improved (P<0.05) the jejunum weight and the lengths of the jejunum, cecum, and proventriculus. Early post hatching erythrocyte and leukocyte counts also increased significantly, whereas leukocyte differentiation revealed increased immune activity through changes in heterophil, lymphocyte, monocyte, and H/L ratios. These findings indicate that IOF with ATP supports organ development and strengthens immunity and health, suggesting a promising approach to improve the quality of day-old chicks and productivity in local poultry farming.
Influence of In-Ovo ATP Injection on Hatching Performance, Morphometry, and Carcass Traits in Sentul Chickens under Moderate-Latitude Conditions Shiddiq, Muhammad Rasyid; Ulfah, Maria; Anggraini, Lili; Krisnan, Rantan
Jurnal Ilmu-Ilmu Peternakan Vol. 36 No. 1 (2026): April 2026
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/

Abstract

In this study, the effects of in ovo injection with adenosine triphosphate (ATP) on the hatching performance, body measurements, and carcass characteristics of Sentul chickens, an indigenous breed of Indonesia, were evaluated. ATP, a key nucleotide that mediates cellular energy transfer, was injected into the amniotic cavity on day 18 of incubation. Unsex 250-day-old chicks (DOCs) were subjected to two treatments: control (P1: without ATP injection) and ATP injection on the 18th day of incubation (P2). The birds were maintained in three replicates per treatment, and the cage was considered the experimental unit. During the rearing period, the birds were redistributed according to three growth stages: period I (1–14 days, 2 cages, 125 chicks per cage), period II (15–28 days, 6 cages, 30 birds per cage), and period III (29–56, 12 cages, 15 birds per cage). The data were analysed using an independent t test, and differences were considered significant at P<0.05. ATP injection slightly improved hatchability (92,8 ± 0,71) and hatching weight (33,12 ± 0,56) compared with those of the control group (91,2 ± 0,93 and 32,50 ± 0,66, respectively), although the differences were not statistically significant (p>0,05). However, compared with the control treatment, the ATP treatment significantly improved several morphometric traits, including tibia length in Period I (4,60 ± 0,40 vs, 3,78 ± 0,70; P<0.05) and wing and chest lengths across the observation periods (p<0.05). At 8 weeks of age, compared with the control group (404.50 ± 196.49 g; P < 0.05), the group receiving ATP injection had a higher carcass weight (474.58 ± 206.22 g), whereas the carcass percentage and the meat-to-bone ratio were not significantly affected (P > 0.05). These findings indicate that in ovo ATP injection may increase the skeletal growth and carcass weight of Sentul chickens without affecting hatchability or carcass composition.