Ayda Krisnawati
Indonesian Legume and Tuber Crops Research Institute (ILETRI), Malang, Indonesia

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Journal : BERITA BIOLOGI

HETEROSIS, HETEROBELTIOSIS DAN TINDAK GEN KARAKTER AGRONOMIK KEDELAI {Glycine max (L.) Merrill} Krisnawati, Ayda; Adie, MM
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1022.153 KB) | DOI: 10.14203/beritabiologi.v10i6.1952

Abstract

Most of the soybean {Gfycine max (L.) Merrill (varieties in Indonesia are produced through hybridization. Expected value of heterobeltiosis,heterosis and the gene action is needed to know to optimize the development strategy of soybean varieties. The experiment was conducted in The Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian (Balitkabi) greenhouse. The first stage was to form Fl hybrid seed,which was done from May to July 2009; the second stage was for the assessment of Fl plants (August-November 2009). Heterosis,heterobeltiosis and the potential ratio are calculated from the 18 crosses combinations derived from 6 parental genotypes. Data used include the flowering day (days), maturity day (days), plant height (cm), number of branches/plant, number of node/plant, number of pods/plant,seed yield/plant (g) and SO seeds weight (g). Cross combinations that have the highest heterosis values are on the flowering day, maturity day, number of branches/plant and number of node/plant; heterobeltiosis also showed the highest value on the same agronomic characters.Heterosis value of the character of seeds per plant ranged from -36.50% to 75.49%, while for heterobeltiosis ranged from -48.68% to 58.31%. The highest heterosis value was indicated by the Fl from crosses Willis x Malabar. The highest heterobeltiosis value was indicated by crosses of Willis x Grobogan, which means that these crosses have the 58.31% higher yield than the best parents. The soybean yield is controlled by dominant genes and over-dominant. Combination crosses Willis X MLG 0706 (reciprocal) has a higher potential ratio for die character of the number of pods and seeds yield/plant compared to the value of the other potence ratio. Development of soybeans with the use of hybrid heterosis phenomenon still needs to be assessed in terms of economic feasibility.
PENGARUH UMUR MASAK POLONG TERHADAP VIABILITAS DAN VIGOR BENIH BEBERAPA AKSESI BENGKUANG (Pachyrhizus erosus) Krisnawati, Ayda; Adie, M Muchlish
BERITA BIOLOGI Vol 17, No 3 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5171.137 KB) | DOI: 10.14203/beritabiologi.v17i3.3237

Abstract

Seed germination uniformity and simultaneity of yam bean was related to pod maturity. A total of ten accessions of yam bean was planted at Kendalpayak Research Station, Malang (Indonesia) from May to October 2016. Yam bean pods of each accession were harvested at three developmental stages, i.e. full-size green pods, yellow pods, and brown/black pods. The experiment was conducted using a completely randomized factorial design with three replications. The first factor was pod maturity and the second factor was yam bean accessions. Significant interactions between pod maturities with accessions were found on characters of epicotyl length, leaf length, leaf width, root dry weight, maximum growth potential, and germination rate at the 10th days of observation. The pod maturity at yellow stage gave the highest maximum growth potential and germination rate. The average germination rate of yellow pods was 8.15% per day which was, higher than those of green pods (3.83% per day) as well as black pods (3.73% per day). These yellow pods have optimal germination growth of compared to those of green as well as black pods. The practical implication of this research is the use of yellow pods as recommended seed source for yam bean cultivation. 
HASIL DAN STABILITAS HASIL BIJI KEDELAI {GLYCINE MAX (L.) MERR.} GALUR HARAPAN DI LAHAN SAWAH Krisnawati, Ayda; Adie, M Muchlish; Susanto, GWA
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.524

Abstract

Soybean {Glycine max (L.) Merr.} varieties with consistently high yield productivity across environments are expected to maintain its production level per area.The objectives of this experiment are to determine the magnitude of G × E interaction and to identify the stability of eight soybean promising lines across locations. Materials consists of eight soybean promising lines (G100H/SHRW-60-38, SHRW-60/G100H-73, SHRW-60/G100H-68, SHRW-60/G100H-66, G100H/SHRW-34, SHRW-60/G100H-5, SHRW-60/G100H-70 and SHRW-60/G 100 H-75) and two check varieties (Kaba and Wilis). The experiments were done in 16 locations (Lampung Tengah, Yogyakarta, Ngawi, Mojokerto, Pasuruan, Malang, Banyuwangi and Lombok Barat, two locations each) during the period of 2009 to 2011. A randomized completely block design with four replicates was used in each location. AMMI analysis (Additive Main Effects and Multiplicative Interaction) was applied to assess the yield stability of those 10 genotypes, and then interpreted in biplot graphic of seed yield for principal components 1 (IPCA1) with the principal component 2 (IPCA2). Seed yield of the 10 soybean lines ranged from 2.63-3.02 t/ha, with 2.81 t/ha in average. The highest yield was obtained by G6 (SHRW-60/G100H-5), whereas G3 (SHRW-60/G100H-68) had the lowest seed yield.The combined analysis showed that lines, locations, and the interaction of lines and locations (G × L) were significantly different for seed yield.The first four Interaction Principal Component Axes (IPCA1, IPCA2, IPCA3 and IPCA4) were significant and accounted for 85.1% of the total GEI. Lines of G100H/SHRW-60-38 (G1), SHRW-60/G100H-66 (G4) and SHRW-60/G100H-5 (G6) were stable and high yielding, and therefore they are proposed to be released as new varieties. The results of this study also suggested that Kaba and Willis were used as specific-check varieties, due to its site-specific adaptability.
AGRONOMIC CHARACTERS OF DROUGHT-TOLERANT SOYBEANS AT THE REPRODUCTIVE STAGE Adie, M. Muchlish; Krisnawati, Ayda
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3365

Abstract

In Indonesia, soybeans are typically cultivated during the dry season, thus making it prone to drought stress in the reproductive stage. The objective of the research was to characterize the agronomic characters of several soybean genotypes which were tolerant to drought at the reproductive stage. A total of 19 soybean genotypes were evaluated for its agronomic characters and tolerance to drought stress in Probolinggo (East Java, Indonesia) during the dry season (June ? September). The research was arranged in a randomized block design with four replicates. Soybean cultivars were sown at two separate experiments, normal/optimal (plants were irrigated during the growth period) and stress (plants were irrigated up to 40 days after planting) conditions. Drought stress during the reproductive stage did not affect the characters of plant height, the number of branches per plant, the number of nodes per plant, and the number of pods per plant. Meanwhile, the number of empty pod, seed weight per plant, and seed yield were significantly affected. There were two genotypes which were identified as the tolerant genotypes to drought stress at the reproductive stage, i.e. MDT15-6-11 and MDT15-W-3034. The agronomic characters of MDT15-6-11 were showed as having a low percentage of yield reduction, hence it was able to mantain its high yield. The MDT15-W-3034 was identified as drought-tolerant due to the slow wilting, high plant character, a slow vegetative phase, and high yields. The genotypes obtained from this study could be recommended to be released as new soybean drought-tolerant varieties due to its high yield and tolerant to drought stress. Those genotypes could also be used as gene donors for soybean improvement to drought stress at the reproductive stage.  
INTERAKSI GALUR × LINGKUNGAN, POTENSI HASIL DAN STABILITAS HASIL GALUR HARAPAN KEDELAI {GLYCINE MAX (L.) MERR.} HITAM Adie, M Muchlish; Krisnawati, Ayda; Susanto, GWA
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.520

Abstract

Recent demand of black soybean {Glycine max (L.) Merr.} seed has been rising for industrial raw material. A total of five promising lines of black soybean (9837/K-D-8-185, 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211) were evaluated for the potential yield at 18 soybean production centrals in West Java, East Java, Bali and West Nusa Tenggara, from 2004 until 2006..Variety of Cikuray (black soybean), Wilis (famous variety with high yield) and Burangrang (large seed size and short maturation day) were used as check varieties.The field experimental design for each location was completely randomized design with four replicates.The treatment consist of eight lines/variety with 2,4 m × 4,5 m plot size, 40 cm × 15 cm plant distance, two plants/hill.Fertilizer of 50 kg Urea, 100 kg SP36 and 75 kg KCl per ha were applied before sowing time. Weed, insect and disease were controlled intensively.Stability analysis was according to Eberhart and Russell (1966), and adaptability was by Finlay dan Wilkinson (1963). Location, genotype and interaction between 2genotype x environment (G × L) were significant for seed yield. Estimate of environment variance component (d L = 0.0604) was the 2 2 biggest one, and followed by error variance value (d E = 0.0470), genotype variance (d G = 0.0258) and the smallest was estimate of 2 interaction variance G × L (d GL = 0.0225).Significant interaction of G × L showed that each promising lines have specific reaction to certain location quality. Five promising lines were able to produce 2.44 until 2.51 t/ha, or 17% higher than black soybean variety of Cikuray (2.03 t/ha); or 10% higher than Burangrang and 3% more higher than Wilis. Genotype of 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211 were indentified stable. Genotype of 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211 were promising to be high yielded and adaptable across location; on the contrary, genotype of 9837/K-D-8-185 was more adaptable in optimal location.