Ayda Krisnawati
Indonesian Legume and Tuber Crops Research Institute (ILETRI), Malang, Indonesia

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PENCEMARAN KADMIUM DAN PROSPEK PEMULIAAN TANAMAN KEDELAI BERKANDUNGAN KADMIUM RENDAH Krisnawati, Ayda
Buletin Palawija No 26 (2013)
Publisher : Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian

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Abstract

Kadmium (Cd) merupakan logam berat pencemar lingkungan yang bersifat toksik. Kadmium dapat terakumulasi dalam kadar yang tinggi pada bagian tanaman kedelai (Glycine max L. Merr.) yang bila dikonsumsi akan berisiko pada kesehatan manusia, sehingga diperlukan usahausaha untuk membatasi akumulasi kadmium dalam tanaman. Penggunaan varietas unggul yang memiliki kemampuan mengakumulasi kadmium dalam konsentrasi yang rendah merupakan salah satu solusi yang cukup rasional. Varietas unggul merupakan komponen teknologi yang efisien dan ramah lingkungan dibandingkan dengan upaya perbaikan kondisi lingkungan. Di Indonesia, penelitian pemuliaan belum mengarah pada pencemaran kadmium pada tanaman kedelai. Namun dengan adanya ekstensifikasi pertanian dan sumber pangan sehat yang menyehatkan, maka penelitian tentang kadmium pada tanaman kedelai akan menjadi informasi yang dapat bermanfaat dalam perakitan varietas kedelai berkandungan kadmium rendah. Variabilitas genetik, pemilihan teknik skrining dengan kriteria seleksi yang tepat, pemahaman mekanisme ketahanan disertai dukungan pengetahuan level molekuler merupakan elemen penting dalam meraih keberhasilan perakitan kedelai berkandungan kadmium rendah.
HETEROSIS, HETEROBELTIOSIS DAN TINDAK GEN KARAKTER AGRONOMIK KEDELAI {Glycine max (L.) Merrill} Krisnawati, Ayda; Adie, MM
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1022.153 KB) | DOI: 10.14203/beritabiologi.v10i6.1952

Abstract

Most of the soybean {Gfycine max (L.) Merrill (varieties in Indonesia are produced through hybridization. Expected value of heterobeltiosis,heterosis and the gene action is needed to know to optimize the development strategy of soybean varieties. The experiment was conducted in The Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian (Balitkabi) greenhouse. The first stage was to form Fl hybrid seed,which was done from May to July 2009; the second stage was for the assessment of Fl plants (August-November 2009). Heterosis,heterobeltiosis and the potential ratio are calculated from the 18 crosses combinations derived from 6 parental genotypes. Data used include the flowering day (days), maturity day (days), plant height (cm), number of branches/plant, number of node/plant, number of pods/plant,seed yield/plant (g) and SO seeds weight (g). Cross combinations that have the highest heterosis values are on the flowering day, maturity day, number of branches/plant and number of node/plant; heterobeltiosis also showed the highest value on the same agronomic characters.Heterosis value of the character of seeds per plant ranged from -36.50% to 75.49%, while for heterobeltiosis ranged from -48.68% to 58.31%. The highest heterosis value was indicated by the Fl from crosses Willis x Malabar. The highest heterobeltiosis value was indicated by crosses of Willis x Grobogan, which means that these crosses have the 58.31% higher yield than the best parents. The soybean yield is controlled by dominant genes and over-dominant. Combination crosses Willis X MLG 0706 (reciprocal) has a higher potential ratio for die character of the number of pods and seeds yield/plant compared to the value of the other potence ratio. Development of soybeans with the use of hybrid heterosis phenomenon still needs to be assessed in terms of economic feasibility.
Evaluation of Soybean Resistance to Pod-Sucking Bug, Riptortus linearis F. and Performance of its Agronomic Characters Krisnawati, Ayda; Adie, Mochammad Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12806

Abstract

Pod sucking bug, Riptortus linearis, is the most damaging soybean pest in Indonesia. A total of 24 soybean genotypes was identified for their resistance to pod sucking bug. The objective of the study was to classifying the resistance of some soybean genotypes against pod sucking pests. The research was conducted during the second dry season (July - October) 2016 in Ngale Research Station, East Java, Indonesia. The experimental design was arranged in randomized block design with five times repetition and two types of environment (full crop protection and controlled until 50 days of planting/dap). The resistance to pod sucking bug was evaluated based on seed damage, pod damage, and seed weight. The grouping of resistance was by using Chiang & Talekar’s method (1980). A very high natural population of pod sucking bug was recorded in the research area as seen from the average number of damaged pod in full protection environment (L1) and insecticide control until 50 dap (L2), i.e. 41.45% and 60.16%, respectively. Genotype of G511H/Anj//Anj-2-8 was consistently resistant to pod sucking bug in L1 as well as L2. This genotype also had early days to maturity (78 days) and large seed size (15.57 g/100 seed), thus potentially to be developed in tropical area of Indonesia. The availability of genotype with such characteristics is in accordance with farmers’ preference and also important to minimize yield losses.
Identification of Soybean Resistance to Pod Sucking Bug (Riptortus linearis) by No-Choice Test Krisnawati, Ayda; Bayu, Marida Santi Yudha Ika; Adie, Moch. Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5180

Abstract

Soybean pod damaged by pod sucking bug (Riptortus linearis) is one of the constraint within soybean yield improvement in Indonesia. The research aimed was to identify the resistance of soybean genotypes to pod sucking pest. The experiment was conducted in Iletris screen house from March to June 2015. The experiment was arranged in Randomized Completely Block Design using 10 soybean genotypes with three replicates. The soybean resistance was evaluated using no-choice test. Data collected on number of pod/plant, number of seed/plant, seed weight per plant, number of attacked pod/plant, and number of attacked seed/plant. The result showed that the lowest percentage both of pod and seed damage was G511H/Anjasmoro//Anjasmoro-2-8, that was 25.83 % and 19.12 %, respectively. Based on the value of the percentage of seed damage, there were five susceptible genotypes, three moderately resistant, and two resistant genotypes. Based on the value of the percentage of pod damage, showed four susceptible genotypes, five moderately resistant, and a resistant genotype. G511H/Anjasmoro//Anjasmoro-2-8 was the only resistant genotype, and it could be used as a genetic source in the improvement of soybean resistance to pod sucking bug.How to CiteKrisnawati, A., Bayu, M. S. Y. I. & Adie, M. M. (2016). Identification of Soybean Resistance to Pod Sucking Bug (Riptortus linearis) by No-Choice Test. Biosaintifika: Journal of Biology & Biology Education, 8(3), 407-414.
Identification of Soybean Genotypes for Pod Shattering Resistance Associated with Agronomical and Morphological Characters Krisnawati, Ayda; Adie, Mochammad Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8722

Abstract

A yield loss caused by pod shattering is one of the obstacles to the improvement of soybean productivity in tropical areas. The aim of this study was to identify the resistance of soybean genotypes to pod shattering as affected by agronomical and morphological characters. The field study was conducted in Malang, Indonesia, using 150 soybean genotypes. Data were collected on agronomical traits, the percentage of pod shattering, and pod morphological traits. Identification for shattering resistance was done as per oven dry method. Percentage of pod shattering was ranged from 0 % up to 100 % shattering with a mean of 58.11 %. Pod shattering was found to be negatively correlated with a number of pod per plant, the thickness of the pod, and Y/Z (seed weight and pod weight ratio). The Identification obtained 66 very highly susceptible genotypes, 19 susceptible genotypes, 19 moderate genotypes, 38 resistant genotypes, and 8 very resistant genotypes. Two of eight very resistant genotypes (G511H/Anj//Anj///Anj////Anj-6-11 and G511H/Anj//Anj///Anj////Anj-5-4) have high yield, medium maturity day and large seed size. Those lines could be used as gene donor for soybean varietal improvement for shattering resistance, and recommended to propose as new improved soybean varieties resistant to pod shattering in Indonesia.
Diversity in Tuber Characteristics of Local Cultivars of Yam Bean (Pachyrhizus erosus) in Indonesia Krisnawati, Ayda; Sutrisno, Sutrisno; Adie, Mochammad Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14272

Abstract

Yam bean is legume with tuberous root originated from the semiarid tropics of Central America, and has been widely spread in several regions in Indonesia. Research on characterization of tuber yam bean was conducted in Kendalpayak Research Station (Malang, Indonesia) from May to October 2016. A total of 73 local yam bean cultivars originated from eight provinces were characterized based on their tuber characteristics. The sensory evaluation was also conducted to evaluate the tuber sweetness, fiber intensity, and texture crunchiness. The average tuber weight was 455.2 g/plant, with the average tuber length and width were 10.78 cm and 8.17 cm, respectively. The ratio of tuber length and width was 1.42, indicates that yam bean tuber in Indonesia tend to be in spherical shape. The average tuber water content was 83.87% (wet basis), showed that the water content was quite high. Based on cluster analysis, 74 local cultivars of yam bean were grouped into five clusters. The tuber weight varied among cultivars and become the distinguishing factor between local cultivars. Local variety of yam beans in Indonesia tend to have sweet taste and crunchy texture. So far, the study on characterization of tuber diversity in Indonesian local cultivar of yam bean is still limited. Thus, these results provide important information to optimize the tuber yam utilization as industrial raw materials.
PENGARUH UMUR MASAK POLONG TERHADAP VIABILITAS DAN VIGOR BENIH BEBERAPA AKSESI BENGKUANG (Pachyrhizus erosus) Krisnawati, Ayda; Adie, M Muchlish
BERITA BIOLOGI Vol 17, No 3 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5171.137 KB) | DOI: 10.14203/beritabiologi.v17i3.3237

Abstract

Seed germination uniformity and simultaneity of yam bean was related to pod maturity. A total of ten accessions of yam bean was planted at Kendalpayak Research Station, Malang (Indonesia) from May to October 2016. Yam bean pods of each accession were harvested at three developmental stages, i.e. full-size green pods, yellow pods, and brown/black pods. The experiment was conducted using a completely randomized factorial design with three replications. The first factor was pod maturity and the second factor was yam bean accessions. Significant interactions between pod maturities with accessions were found on characters of epicotyl length, leaf length, leaf width, root dry weight, maximum growth potential, and germination rate at the 10th days of observation. The pod maturity at yellow stage gave the highest maximum growth potential and germination rate. The average germination rate of yellow pods was 8.15% per day which was, higher than those of green pods (3.83% per day) as well as black pods (3.73% per day). These yellow pods have optimal germination growth of compared to those of green as well as black pods. The practical implication of this research is the use of yellow pods as recommended seed source for yam bean cultivation. 
Pengaruh Posisi Biji Pada Polong Terhadap Perkecambahan Benih Beberapa Varietas Lokal Bengkuang (Pachyrizus erosus L.) Krisnawati, Ayda; Adie, M. Muchlish
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1240.829 KB) | DOI: 10.14203/jbi.v14i2.3736

Abstract

 ABSTRACTThe yam bean seeds are formed in relatively long pods containing between 6-12 seeds/pod. A total of six local yam bean accessions from Indonesia was studied for its seed position in pod related to seed viability and vigor. Field research was carried out in Malang (Indonesia) from May - October 2016. Twenty five matured pods were detached from each accession. Each pod was divided into three parts, i.e. base, middle, and tip part. The seeds of each part are mixed and taken as many as 20 seeds for germinating. The design was a completely randomized design with two factors and repeated three times. The first factor was three seed positions (base, middle, and tip) and the second factor was six accessions of Indonesian local yam bean. The analysis of variance showed that seed position had significant effect on individual seed weight, leaf length, leaf width, leaf shape index, seedling dry weight, and root dry weight. Seeds derived from the midde of the pods produce the highest leaf length, leaf width, leaf shape index, seedling dry weight, and root dry weight. The parameters of seed viability and vigor were not affected by the seed position in the pods. Therefore, the seed viability and vigor of yam bean plant in the field can be produced from the seeds derived from the tip, middle and base of the pod. Keywords: seed position, viability, vigor, yam bean  
The Effect of Seed Position in Pod on The Seed Viability of Cowpea (Vigna unguiculata) Adie, Mochammad Muchlish; Krisnawati, Ayda; Hapsari, Ratri Tri
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.943 KB) | DOI: 10.15294/biosaintifika.v11i1.17730

Abstract

In Indonesia, cowpea (Vigna unguiculata L. Walp) is widely cultivated in the dry climate area. Famers used the seeds from previous plants or traditional markets. The aims of the study were to determine the effect of seed position in the pods on seed viability and to study the seed and seedling characteristics regarding the genetic variation and seed position. The cowpea used in this study was from 18 Indonesian local varieties. Twenty-five matured pods were randomly detached from each variety. Each pod was divided into three parts, i.e. the lower third (base), the middle third, and the upper third (tip). The seeds of each part were germinated using sand media to evaluate their viability. Each local variety has different response to the percentage of seed viability in each seed position in the pod. The highest abnormal germination occurred in the seed from lower part of the pod, followed by seed in the middle of the pod and the tip of the pod. The variation of seed viability in pods was more determined by genetic differences in local varieties. The average seed viability from three parts of the pods was highest for seeds originated from local varieties of Lumajang and Sampang. Therefore, it is recommended that the seeds of both local varieties can be mixed to be used for optimum cowpea cultivation. 
HASIL DAN STABILITAS HASIL BIJI KEDELAI {GLYCINE MAX (L.) MERR.} GALUR HARAPAN DI LAHAN SAWAH Krisnawati, Ayda; Adie, M Muchlish; Susanto, GWA
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.524

Abstract

Soybean {Glycine max (L.) Merr.} varieties with consistently high yield productivity across environments are expected to maintain its production level per area.The objectives of this experiment are to determine the magnitude of G × E interaction and to identify the stability of eight soybean promising lines across locations. Materials consists of eight soybean promising lines (G100H/SHRW-60-38, SHRW-60/G100H-73, SHRW-60/G100H-68, SHRW-60/G100H-66, G100H/SHRW-34, SHRW-60/G100H-5, SHRW-60/G100H-70 and SHRW-60/G 100 H-75) and two check varieties (Kaba and Wilis). The experiments were done in 16 locations (Lampung Tengah, Yogyakarta, Ngawi, Mojokerto, Pasuruan, Malang, Banyuwangi and Lombok Barat, two locations each) during the period of 2009 to 2011. A randomized completely block design with four replicates was used in each location. AMMI analysis (Additive Main Effects and Multiplicative Interaction) was applied to assess the yield stability of those 10 genotypes, and then interpreted in biplot graphic of seed yield for principal components 1 (IPCA1) with the principal component 2 (IPCA2). Seed yield of the 10 soybean lines ranged from 2.63-3.02 t/ha, with 2.81 t/ha in average. The highest yield was obtained by G6 (SHRW-60/G100H-5), whereas G3 (SHRW-60/G100H-68) had the lowest seed yield.The combined analysis showed that lines, locations, and the interaction of lines and locations (G × L) were significantly different for seed yield.The first four Interaction Principal Component Axes (IPCA1, IPCA2, IPCA3 and IPCA4) were significant and accounted for 85.1% of the total GEI. Lines of G100H/SHRW-60-38 (G1), SHRW-60/G100H-66 (G4) and SHRW-60/G100H-5 (G6) were stable and high yielding, and therefore they are proposed to be released as new varieties. The results of this study also suggested that Kaba and Willis were used as specific-check varieties, due to its site-specific adaptability.