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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Pediatric Sciences Journal
Krisni Subandiyah
Department of Child Health, Faculty of Medicine, University of Brawijaya
Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Public Health

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 1 
Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome Tikto Wahyono; Krisni Subandiyah; Loeki Enggar Fitri; Agustina Tri Endharti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

This study aimed to prove the difference of TLR4 and subunit p65NF-kB expression between children with steroid-sensitive nephrotic syndrome (SSNS) and those with steroid-resistant nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR4 and p65NF-kB on children with SRNS. Twenty three patients of idiopathic nephrotic syndrome patients (INS) were enrolled in this study and classified into two groups—those with SSNS (11 patients) and SRNS (12 patients). Peripheral Blood Mononuclear Cells (PBMC) isolation was performed, taken from 5 ml patients blood samples, followed by monocyte isolation of PMBC using monocyte culture. Examination of TLR4 and p65 NF-kB expression from monocyte's cells culture was performed using flow cytometry. All data were processed using Statistical Package for the Social Sciences (SPSS 15) for Windows. From 11 patients that were classified into SSNS group, 81.8% having good nutritional status and 18.2% comprising undernourished status compared to 12 patients of the SRNS group, in which 6 accounting for 41.7% having good nutritional status, 50% having undernourished status and 8.3% having malnourishment status. The average age is 7.59 ± 3.52 and six years old is the highest age studied. Based on normality tests using Shapiro-Wilk, variables of TLR4 have p value of 0.620 and p65 NF-kB have p value of 0.027, showing that the data of TLR4 was distributed normally but p65 NF-kB data was not distributed normally. The test on TLR4 data variation using Levene Test showed its significance value of p was 0.318, meaning that the data was homogeneous. Using unpaired t-test, the differences of TLR4 expression between SSNS and SRNS was significant (p=0.012), while using Mann-Whitney test, the differences of p65 NF-kB expression between SSNS and SRNS showed an insignificant correlation (p=0.880). Pearson correlation test between the expression of TLR4 and p65 NF-kB showed no significant correlation (p=0.472), while Spearman correlation test to see the relationship between expression of TLR4 and SRNS produced correlation value of 0.512 and its importance p value was 0.013 (p
The Correlation Between IL-1β and IL-10 Levels in Estimating The Risk of Febrile Seizures Andi Gunawan; M Muid; Hidayat Suyudi; Barlianto wisnu; Krisni Subandiyah
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Febrile seizures are the most common forms of seizures in children with fever. A data from clinical and experimental studies suggest the potential role of immune generate products in their genesis. The balance between proinflammatory (IL-1β) and anti-inflammatory (IL-10) cytokines influences the regulation of infections and plays a role in the pathogenesis of febrile seizures. In existing literature, there is no research on the correlation between IL-1β and IL-10 levels as well as the ratio of IL-1β to IL-10 in estimating the risk of febrile seizures in seizure prone children aged between 3 months and 5 years. In this study, IL-1β, IL-10 levels and ratio of IL-1β to IL-10 in the risk of febrile seizures were investigated, and respondents were divided into three groups: febrile seizures, febrile without seizures and healthy children without histories of febrile seizure. This study has used A cross-sectional design, and each group had 17 co-respondents. IL-1β and IL-10 levels were measured by ELISA. Data were analyzed by SPSS 15. Findings showed there were significant differences between IL-1β and IL-10 as well as in the ratio of IL-1β to IL-10 between febrile seizure patients and healthy children. It was concluded that there were significant correlations between IL-1β and IL-10 levels as well as IL-1β to IL-10 ratios in estimating the risk of febrile seizures.
Vitamin D3 Adjuvant Treatment Stimulate Interleukin-10 Expression in Children with Nephrotic Syndrome Without Affecting to Clinical Outcome and Glucocorticoid Receptor Expression Husnul Asariati; Krisni Subandiyah; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Idiopathic nephrotic syndrome (INS) is the most glomerular disease that occurred in childhood with high rate morbidity. Glucocorticoid is drug of choice for INS and responsiveness to this drug determined prognosis. Glucocorticoid upregulate transcription of anti-inflammatory cytokines such as IL-4 and IL-10. IL-10 is an anti-inflammatory cytokine and has multiple role in immune response include modulate Th1/Th2 response. Vitamin D3 interact with glucocorticoid signaling. Administered active form of vitamin D3 increase dexamethasone-induced IL-10 expression by regulatory T cells in steroid resistant asthmatic patient. Here we showed increase of CD4+IL10+ expression after treatment both prednisone only and combination prednison with vitamin D3. Both in new-onset NS or rare relaps NS, combination treatment prednisone + vitamin D3 increase CD4+IL10+ expression significantly compared to prednisone-only treated group (p= 0.003), which first group (new-onset nephrotic syndrome + prednisone and vitamin D3 treatment) showed the most CD4+IL10+ expression enhancement (9.53±3.89). However this study failed to show a correlation between CD4+IL-10+ expression after prednisone and vitamin D3 treatment with clinical outcome (linear regression test, p= 0,125). This study also showed that there was a no correlation between CD4+IL-10+ expression and CD3+GR expression after prednison + vitamin D3 treatment (p= 0.088). CD4+IL-10+expression in new-onset and rarely relapsing nephrotic syndrome patients higher in prednisone + vitamin D3 treated group than prednisone-only treated group. There is no correlation between CD4+IL-10+expression and CD3+GR expression nor CD4+IL-10+expression and clinical outcome.
The Correlation of Regulatory T (TReg) and Vitamin D3 in Pediatric Nephrotic Syndrome Yunika Nurtyas; Krisni Subandiyah; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.08

Abstract

Nephrotic syndrome (NS) is an autoimmune disease that correlates to the imbalance of regulatory T cells (TReg). This study was aimed to investigate the effect of vitamin D as adjuvant therapy of TReg population in pediatric nephrotic syndrome. This study was designed randomized clinical trial, double blind, with pre- and post-test control groups involving 15 subjects newly diagnosed with NS. Subjects were divided into 2 groups, namely K1 for group treated with prednisone+vitamin D and K2 group for prednisone treatment only. The population of TReg in peripheral blood mononuclear cells (PBMC) was analyzed using flowcytometry. Vitamin D serum level was measured through ELISA method. Results showed that there was a significant elevation of TReg (independent t-test, p = 0.010) in K1 group, which was higher than in K2 group. The Pearson test in the K1 group showed that vitamin D level was positively correlated with TReg (p = 0.039, r = 0.779).
A successful management of children with retroperitoneal abscess due to recurrent renal staghorn calculi Krisni Subandiyah; Astrid Kristina Kardani; Tita Luthfia Sari
Pediatric Sciences Journal Vol. 5 No. 1 (2024): (Available online: 1 June 2024)
Publisher : Medical Faculty of Brawijaya University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/pedscij.v5i1.46

Abstract

Introduction: A particular kind of urolithiasis known as "staghorn calculi" occurs when the calculus grows into the pelvis and renal calyces. It was characterised by fast growth, which might progress to kidney damage and result in other consequences if left untreated. Although the disease's clinical presentation in paediatric patients might be non-specific, it still dramatically raises morbidity and death rates. This case report aimed to identify the risk factors, clinical manifestation, and treatment of children with retroperitoneal abscesses due to recurrent renal calculi. Case description: We describe a case of a young woman with severe acute malnourishment who had a history of painful swelling in her left renal angle after contracting a urinary tract infection. A radiological examination revealed a massive staghorn calculus in the left kidney with significant hydronephrosis, resulting in a retroperitoneal abscess. The patient had surgical treatment along with free drainage of 1000 millilitres of pus. The systemic antibiotic was given as urosepsis treatment. Percutaneous nephrolithotomy (PCNL) and placement of a double-J catheter were performed as definitive treatment for staghorn calculi three months later. Interestingly, it was the second episode of urolithiasis in this patient in 10 years. Malnourishment and recurrent Urinary Tract Infection (UTI) may be predisposing factors to staghorn calculi and perirenal abscess. Conclusion: Considering its significant problem, early evaluation, treatment, and prevention of recurrence were essential to decrease morbidity and mortality in pediatric urolithiasis.
Co-Authors Agustina Tri Endharti Andi Gunawan Astrid Kristina Kardani Barlianto wisnu Hidayat Suyudi Husnul Asariati Loeki Enggar Fitri M Muid Tikto Wahyono Tita Luthfia Sari Yunika Nurtyas