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All Journal Jurnal Perlindungan Tanaman Indonesia Jurnal HPT (Hama Penyakit Tumbuhan)
Ari Kristini
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Agriculture, Biological Sciences & Forestry

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UJI PENGENDALIAN PENYAKIT POKAHBUNG (Fusarium moniliformae) PADA TANAMAN TEBU (Saccharum officinarum) MENGGUNAKAN Trichoderma sp. INDIGENOUS SECARA IN VITRO DAN IN VIVO Birtha Niken Pratiwi; Liliek Sulistyowati; Anton Muhibuddin; Ari Kristini
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 2 No. 1 (2014)
Publisher : Universitas Brawijaya

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Abstract

Kata Kunci : pokahbung, Fusarium moniliformae, Trichoderma sp., jamur antagonisPenyakit pokahbung yang disebabkan oleh jamur patogen Fusarium moniliformae terdiri dari 3 tingkatan gejala, yaitu pb 1 berupa klorotis pada helaian daun yang baru membuka. Pb 2 berupa garis merah kecoklatan yang meluas menjadi rongga-rongga yang dalam. Pb 3 memiliki gejala spesifik yaitu membengkoknya batang tanaman tebu dan menyerang titik tumbuh yang dapat menyebabkan matinya tanaman tebu. Penggunaan jamur antagonis Trichoderma sp. yang berasal dari tanah di sekitar perakaran tanaman tebu diharapkan mampu mengendalikan penyakit pokahbung. Penelitian dilaksanakan di Laboratorium Penyakit Pusat Penelitian Perkebunan Gula Indonesia Pasuruan. Penelitian dilaksanakan secara in vitro dan in vivo. In vitro dibagi menjadi metode goresan dan penuangan pada media PDA. Suspensi dari jamur antagonis Trichoderma sp. menggunakan kerapatan spora 103, 104 dan 105 sedangkan kerapatan spora jamur patogen F. moniliformae adalah 105. Hasil dari penelitian pada uji antagonis secara in vitro menunjukkan bahwa ketiga kerapatan spora Trichoderma sp. yang digunakan mampu menghambat pertumbuhan F. moniliformae. Pada uji in vivo didapatkan hasil yang berbeda yaitu pada kerapatan spora 103 tidak mampu mengendalikan penyakit pokahbung.Kata Kunci : pokahbung, Fusarium moniliformae, Trichoderma sp., jamur antagonis
Sugarcane streak mosaic virus (ScSMV) Resistance Evaluation of Sugarcane Varieties Ernawati, Feny; Djauhari, Syamsuddin; Martosudiro, Mintarto; Putra, Lilik Koesmihartono; Kristini, Ari
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71158

Abstract

Sugarcane streak mosaic virus (ScSMV) is the most important viral disease of sugarcane in Indonesia with distribution in almost all commercial sugarcane plantations. The disease causes significant yield losses of both cane tonnage and sugar yield. The use of resistant varieties is the best approach for controlling viral diseases. This study aims to investigate resistance response of several introduced varieties against ScSMV in a glasshouse condition and the impact of the viral infection on chlorophyll and proline content in sugarcane leaves.  Sugarcane plants were inoculated using ScSMV inoculum one month after planting using an abrasive pad rubbing method. Disease incidence and severity was observed at week 4-12 after inoculation and variety resistance levels were classified based on disease incidence. Confirmation of the virus was done by RT-PCR. Spectrophotometer was used to measure chlorophyll content at dual wavelengths of 645 and 663 nm, and proline content at wavelengths of 520 nm. The results showed that most of the tested varieties were susceptible to ScSMV. There are six highly resistant varieties, namely SRA 1, SRA 2, N 10-4, N 10-7, N10-9, and N 10-13, but these varieties still require to be tested on a field scale. ScSMV infection generally decrease chlorophyll and proline content. However, the physiological effect of ScSMV infection on chlorophyll and proline content needs further investigation.
Co-Authors Anton Muhibuddin Birtha Niken Pratiwi Birtha Niken Pratiwi Ernawati, Feny Liliek Sulistyowati Liliek Sulistyowati Lilik Koesmihartono Putra Mintarto Martosudiro Syamsuddin Djauhari