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All Journal Jurnal Perlindungan Tanaman Indonesia Indonesian Sugar Research Journal
Lilik Koesmihartono Putra
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Electrical & Electronics Engineering Energy Environmental Science

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Efikasi Beberapa Isolat Jamur Metarhizium anisopliae Terhadap Hama Uret Lepidiota stigma F. (Coleoptera: Scarabaeidae) di Laboratorium Wiwit Wicaksono Jati; Etik Mar’ati Achadian; Dendi Juliadi; Lilik Koesmihartono Putra
Indonesian Sugar Research Journal Vol 1, No 2 (2021): Indonesian Sugar Research Journal
Publisher : Pusat Penelitian Perkebunan Gula Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (803.025 KB) | DOI: 10.54256/isrj.v1i2.53

Abstract

Serangan hama uret Lepidiota stigma F. dapat menyebabkan kehilangan hasil tebu mencapai 30%, dan pada serangan berat dapat menyebabkan gagal panen.  Pengendalian hama dengan aplikasi insektisida sintetik secara terus menerus berpotensi menimbulkan dampak negatif terhadap keseimbangan ekosistem antara lain pencemaran lingkungan. Pengendalian secara biologis dengan jamur entomopatogenik merupakan alternatif teknik pengendalian yang dapat dikembangkan. Oleh karena itu tujuan penelitian ini adalah untuk mengetahui kemampuan 5 isolat jamur Metarhizium anisopliae yang diperoleh dari beberapa instansi untuk pengembangan agen hayati hama uret di wilayah Purworejo.  Penelitian uji efikasi dilakukan dalam 2 tahap. Uji efikasi tahap 1 dilakukan pada 5 isolat  terhadap uret dari Purworejo. Adapun uji efikasi tahap 2 dilakukan pada 2 isolat terpilih dengan penyetaraan kerapatan spora. Hasil penelitian menunjukkan  bahwa terdapat 2 isolat yang mampu menyebabkan kematian hama uret yaitu isolat M1 dan M5. Isolat M5 menunjukkan hasil uji efikasi tahap pertama yang lebih tinggi dibandingkan isolat lainnya. Hasil uji efikasi tahap 2 antara isolat jamur M. anisopliae M1 dan M5, keduanya efektif untuk mengendalikan hama uret L. stigma.
Sugarcane streak mosaic virus (ScSMV) Resistance Evaluation of Sugarcane Varieties Ernawati, Feny; Djauhari, Syamsuddin; Martosudiro, Mintarto; Putra, Lilik Koesmihartono; Kristini, Ari
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71158

Abstract

Sugarcane streak mosaic virus (ScSMV) is the most important viral disease of sugarcane in Indonesia with distribution in almost all commercial sugarcane plantations. The disease causes significant yield losses of both cane tonnage and sugar yield. The use of resistant varieties is the best approach for controlling viral diseases. This study aims to investigate resistance response of several introduced varieties against ScSMV in a glasshouse condition and the impact of the viral infection on chlorophyll and proline content in sugarcane leaves.  Sugarcane plants were inoculated using ScSMV inoculum one month after planting using an abrasive pad rubbing method. Disease incidence and severity was observed at week 4-12 after inoculation and variety resistance levels were classified based on disease incidence. Confirmation of the virus was done by RT-PCR. Spectrophotometer was used to measure chlorophyll content at dual wavelengths of 645 and 663 nm, and proline content at wavelengths of 520 nm. The results showed that most of the tested varieties were susceptible to ScSMV. There are six highly resistant varieties, namely SRA 1, SRA 2, N 10-4, N 10-7, N10-9, and N 10-13, but these varieties still require to be tested on a field scale. ScSMV infection generally decrease chlorophyll and proline content. However, the physiological effect of ScSMV infection on chlorophyll and proline content needs further investigation.
Co-Authors Ari Kristini Dendi Juliadi Ernawati, Feny Etik Mar’ati Achadian Mintarto Martosudiro Syamsuddin Djauhari Wiwit Wicaksono Jati