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Purification and characterization of recombinant Streptokinase expressed in E.coli from Streptococcus equisimilis with N-terminal methionine Vadla, Pavan Kumar; Tummuru, Murali; Kumar, Dinesh
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Streptokinase is a extracellular enzyme which is extracted from strains of beta Hemolytic streptococcus. The enzyme is a non-protease plasminogen activator that activates plasminogen to plasmin and degrades fibrin clot through its specific lysine binding site which is used in thrombolytic therapy. Purification of streptokinase produced from S.equisimilis in E.coli with N-terminal methionine was carried out in 3 Chromatography purification steps, 1) CM-Sepharose-FF at pH 4.2 followed by concentration and dialysis over night with Tris-HCl pH 8.0. Partially purified dialyzed enzyme sample was loaded on to 2) DEAE-Sepharose-FF column. The Purified fractions of DEAE column were pooled and applied on to Sephadex G-100 column. Enzyme purity was confirmed by SDS-PAGE and RP-HPLC.Its biological activity is determined by specific streptokinase assay and characterised the enzyme by Peptide mapping, MALDI-TOF, Isoelectric-focusing and RP-HPLC. The isoelectric point (pI) of streptokinase is around 4.98.The results of characterization shows that it contains two forms (Isomers) of streptokinase expressed in E. coli which was analyzed by RP-HPLC and chromogenic assay. The variation is formed by isomer-1 in which 85% of Streptokinase expressed without methionine (85000IU/mg) and Isomer-2 in which 15% of streptokinase expressed with methionine (nil activity) in E. coli. This phenomenon shows that the presence and absence of methionine in isomers of streptokinase varying the catalytic activity of the enzyme.

Need for Revamping Information Technology Laws in India Walia, Ivneet Kaur; Kumar, Dinesh
Brawijaya Law Journal : Journal of Legal Studies Vol 8, No 2 (2021): State Administration Role in Establishing Constitutional Obligation
Publisher : Faculty of Law, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.blj.2021.008.02.03

Homo Sapiens have a distinct characteristic of being superior to other creatures. They owe this superiority not only because they have the power to reason and rationalize but also because they have a tendency to organize themselves as a congregation, which can work in a group at a large scale. The human instinct to improvise on its own inventions, have today resulted in mutation of a world from the stage of abacus to the era of Robotics. For the sake of avoiding chaos and maintaining the sovereignty, every nation strives to eradicate the fear of dominance by the selected elite and hence the need of regulations and the law. The aim of the paper is to highlight the grey areas and limitations existent in Information Technology Laws and focusing on the emerging domains of cyberspace. Its also aims to draw the attention by the policymakers and the legislators to understand the need for amending the Information Technology Act, 2000 for including legal provisions related to emerging issues in cyber space. The analytical research method is used to collect the data based on a systematic review of the existing sources of information and involved qualitative research to analyze the information. The conclusion and suggestions of this paper will definitely be helpful in either drafting or amending a comprehensive law relating to IT keeping in view the evolving technologies and their applications.

Need for Revamping Information Technology Laws in India Walia, Ivneet Kaur; Kumar, Dinesh
Brawijaya Law Journal Vol. 8 No. 2 (2021): State Administration Role in Establishing Constitutional Obligation
Publisher : Faculty of Law, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.blj.2021.008.02.03

Homo Sapiens have a distinct characteristic of being superior to other creatures. They owe this superiority not only because they have the power to reason and rationalize but also because they have a tendency to organize themselves as a congregation, which can work in a group at a large scale. The human instinct to improvise on its own inventions, have today resulted in mutation of a world from the stage of abacus to the era of Robotics. For the sake of avoiding chaos and maintaining the sovereignty, every nation strives to eradicate the fear of dominance by the selected elite and hence the need of regulations and the law. The aim of the paper is to highlight the grey areas and limitations existent in Information Technology Laws and focusing on the emerging domains of cyberspace. Its also aims to draw the attention by the policymakers and the legislators to understand the need for amending the Information Technology Act, 2000 for including legal provisions related to emerging issues in cyber space. The analytical research method is used to collect the data based on a systematic review of the existing sources of information and involved qualitative research to analyze the information. The conclusion and suggestions of this paper will definitely be helpful in either drafting or amending a comprehensive law relating to IT keeping in view the evolving technologies and their applications.

Advancements in Anode Materials for Cathodic Protection: Nanostructured Alloys, Surface Modifications, and Smart Monitoring Yahaya, Madaniyyu Sulaiman; Nahar, Kapil; Kumar, Dinesh; Usman, Habib Muhammad; Gambo, Abdulhaq Saleh; Umar, Tijjani Aminu; Sulaiman, Mustapha
Buletin Ilmiah Sarjana Teknik Elektro Vol. 6 No. 3 (2024): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/biste.v6i3.11512

This review critically examines advancements in anode materials for cathodic protection systems, focusing on overcoming the limitations of traditional materials like magnesium, zinc, aluminum, graphite, lead-silver alloys, and high-silicon cast iron (HSCI). Conventional anode materials, though widely used, face issues such as rapid degradation, high maintenance costs, and environmental harm. Novel materials, including mixed metal oxides (MMO), advanced aluminum-based alloys, nanostructured materials, and conductive polymers, offer superior electrochemical properties, enhanced durability, and improved performance in aggressive environments like seawater. This review also highlights the role of surface modifications and coatings, such as platinum on titanium and ceramic coatings, in boosting corrosion resistance. Moreover, smart monitoring systems, integrated with IoT and SCADA technologies, are explored for their potential to improve the longevity and efficiency of cathodic protection systems. The paper emphasizes the urgent need for sustainable solutions due to the substantial economic and environmental costs of corrosion, particularly in high-risk industries like oil and gas, maritime, and infrastructure. Future research directions, including the development of hybrid systems combining coatings with CP technologies and the application of advanced alloys and nanostructured materials, are proposed to address the long-term performance and ecological impacts of CP systems.

Thyroid Profile and Serum Lipid Level in Women with Normal Pregnancy Kumar, Raj; Kumar, Sudeep; Arora, Manisha; Kumar, Dinesh; Sah, Sumesh Prasad; Sharma, Shivani
International Journal of Integrated Health Sciences Vol 11, No 2 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v11n2.3460

Objective: To evaluate the changes in thyroid profile and serum lipid level in normal pregnancy.Methods: This observational study was conducted at the Department of Biochemistry of Santosh Medical College, Ghaziabad, UP, India, from June 2021 to February 2022. In this study, 200 average pregnant women were enrolled. The thyroid profile was estimated using the ELISA method, and the lipid profile was measured using the enzymatic kit method. All data were expressed as means and standard deviations, and SPSS version 17 was used for statistical analysis.Results: This observational study observed that the mean T3, T4, and TSH levels increased significantly in the second trimester compared to the first trimester. In contrast, the mean value of T3, T4, and TSH decreased in the third trimester as compared to the second trimester. The mean levels of total cholesterol, triglyceride, and LDL-cholesterol increased significantly, while the mean value of HDL-cholesterol decreased significantly in the second and third trimesters when compared to the first trimester.Conclusion: This study demonstrated abnormal lipid and thyroid metabolism. Changes in thyroid profile may be associated with adverse obstetric outcomes. The altered lipid parameters, mainly High TG and low HDL-C concentrations, may promote vascular dysfunction and oxidative stress.

Role of Osteopontin in Hypothyroid Anemic Woman and Their Association with Oxidative Stress Sah, Sumesh Prasad; Sah, Sonu; Kumar, Sudeep; Arora, Manisha; Kumar, Dinesh; Kumar, Raj
International Journal of Integrated Health Sciences Vol 13, No 1 (2025)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v13n1.4099

Background: Hypothyroidism is frequently associated with anemia and oxidative stress, necessitating exploration of biomarkers like Osteopontin to better understand disease mechanisms.Objective: To explore the association between Osteopontin, oxidative stress, and antioxidant capacity in hypothyroid woman with or without anemia.Methods: The study was conducted at the Santosh Medical College Department of Biochemistry in Ghaziabad, India, from September 2019 to October 2021. This study was cross-sectional and recruited 360 female subjects, divided into three groups: hypothyroidism with anemia, hypothyroidism without anemia, and normal healthy controls. Osteopontin, thyroid profile, malondialdehyde (MDA) and total antioxidant capacity were measured.Results: Osteopontin, T3, T4, and total antioxidant capacity levels were significantly lower (p<0.001), and the TSH and MDA levels significantly increased (p<0.001) in female subjects experiencing hypothyroidism, with or without anemia, as compared to normal healthy female control groups. Osteopontin demonstrated significant negative relationship with TSH and MDA, while showing a significant positive relationship with T3, T4, and total antioxidant status in both studied groups.Conclusion: Osteopontin and oxidative stress/antioxidant status significantly correlate in female subjects experiencing hypothyroidism, with or without anemia. In these patients, Osteopontin might be a useful biomarker for evaluating antioxidant levels and oxidative stress.

Impact of Nutrition Education on Dietary Habits among Bachelor-in-Medicine, Bachelor-in-Surgery (MBBS) Students: A Study in an Indian Medical College Kumar, Sudeep; Sah, Sumesh Prasad; Kumar, Dinesh; Arora, Manish; Sharma, Shiwani
International Journal of Integrated Health Sciences Vol 13, No 2 (2025)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v13n2.4309

Background: Unhealthy eating patterns, often triggered by academic pressures and erratic schedules, can negatively impact students’ health and hinder their ability to effectively guide future patients. Nutrition education offers a valuable opportunity to promote healthier eating habits by providing critical knowledge that supports both students’ personal wellbeing and professional competence.Objective: To evaluate the effect of nutrition education on eating habits of first-year Bachelor-in-Medicine, Bachelor-in-Surgery (MBBS) among medical students.Methods: A pre-post interventional study was carried out on 200 Phase-1 MBBS students of Muzaffarnagar Medical College between January and June 2024. Initial dietary habits were assessed using a validated questionnaire. A structured session of nutrition education was provided. The dietary habits were then re-assessed after four weeks, and changes were analyzed using paired statistical tests.Results: Data collected after the intervention showed notable enhancements in dietary practices. The percentage of students consuming three balanced meals a day increased from 32% to 68% (p < 0.001). The intake of fast food decreased from 65% to 35%, and the consumption of sugar-sweetened beverages fell from 58% to 30%. The average servings of fruits and vegetables consumed daily rose from 2.1 to 4.5 (p < 0.01). Nutrition knowledge scores presented a 30% improvement, indicating greater awareness.Conclusion: Medical students' eating habits are much improved by nutrition education, underscoring its significance in medical education. Future healthcare workers may benefit from lifelong good eating habits if such programs are incorporated into the curriculum, which would improve patient care and their own health.

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