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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Penelitian Pendidikan IPA (JPPIPA)
Marlinda Nilan Sari Rangkuti
Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics

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PERTUMBUHAN KULTUR KONSORTIUM DALAM MINYAK SELAMA BIODEGRADASI DI BAWAH KONDISI AEROB Marlinda Nilan Sari Rangkuti; Palsan Sannasi; Sahilah Abdul Mutalib
JBIO: jurnal biosains (the journal of biosciences) Vol 4, No 1 (2018): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v4i1.9783

Abstract

This study reported the ability of the Consortium Culture (5% v/v) was grown in minimum salts medium (MSM) with the addition of 1% (v / v) crude oil, at different incubation times (0, 2, 4, 6 and 8 weeks). The Consortium Culture (CC) can live and use crude oil as the sole source of carbon and energy. The analysis result shows that the highest percentage of biodegradation is at the second week with the percentage of hydrocarbon residue of 42% and it has been used up in the eighth week. These results indicate that the consortium culture can be utilized as a biodegradation agent and this phenomenon can used to enhance the bioremediation ability of the petroleum hydrocarbon polluted environment.
TEST OF ANTIBACTERIAL ACTIVITY OF THE ETHANOL EXTRACT OF PULUTAN LEAVES (Urena lobata L.) AGAINST Escherichia coli Mhd Yusuf Nasution; Marlinda Nilan Sari Rangkuti; Dina Handayani; Tessa Togatorop; Agnes Adela Br Ginting; Ahmad Shafwan S Pulungan
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 2 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i2.48764

Abstract

ABSTRAK  Tessa Togatorop, NIM 4193520022, Uji Aktivitas Ekstrak Etanol Daun Pulutan Daun Pulutan (Urena lobata L.) Terhadap Bakteri Escherichia coli Dan Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder, uji aktivitas antibakteri ekstrak etanol daun pulutan (Urena lobata L.) dan untuk mengetahui uji Konsentrasi Hambat Minimum (KHM) dan uji Kosentrasi Bunuh Minimum (KBM) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Uji skrining fitokimia dilakukan dengan beberpa pereaksi yang sesuai. Uji aktivitas antibakteri menggunakan metode difusi cakram terhadap bakteri Escherichia coli dan Staphylococcus aureus. Uji KHM dan KBM dilakukan dengan menghitung selisih nilai absorbansi setelah inkubasi dan sebelum inkubasi yang dapat menghambat dan membunuh bakteri uji. Hasil skrining fitokimia esktrak etanol daun pulutan mengandung; flavonoid, saponin, tanin, dan steroid/terpenoid. Hasil uji aktivitas antibakteri dengan metode difusi cakram ekstrak etanol daun pulutan 15%, 25%, 35%, 45%, 55% dan 65% terhadap bakteri Escherichia coli berturut-turut adalah 4,0625 mm, 4,2 mm, 4,85 mm, 3,075 mm, 4,5875 mm, dan 4,3 mm sedangkan pada bakteri Staphylococcus aureus berturut-turut adalah 2,5625 mm, 3,05 mm, 3,35 mm, 3,55 mm, 3,175 mm, dan 4,5 mm. Hasil penelitian menunjukkan ekstrak etanol daun pulutan memiliki aktivitas antibakteri terhadap bakteri Escherhicia coli dan Staphylococcus aureus dalam kategori yang lemah. Uji KHM pada bakteri Escherichia coli adalah konsentrasi 65% dan pada bakteri Staphylococcus aureus adalah konsentrasi 55%. Uji KBM pada bakteri Escherichia coli dan Staphylococcus aureus tidak didapat pada setiap perlakuan konsentrasi. Kata Kunci : Pulutan (Urena lobata L.), Antibakteri, Konsentrasi Hambat Kosentrasi Bunuh Minimum
Potential Microplastic-Degrading Bacteria from Mangrove Sediment in The Paluh Getah Area, Percut Sei Tuan District Ahmad Shafwan S. Pulungan; Larasati Arum Utami; Marlinda Nilan Sari Rangkuti; Ayu Putri Ningsih; Cindy Suci Muliandhira
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12647

Abstract

Microplastic pollution poses a severe threat to marine ecosystems due to its persistence and toxicity, necessitating innovative biodegradation strategies. This study explored the potential of bacteria isolated from mangrove sediments of the Paluh Getah mangrove forest, North Sumatra, to degrade common microplastics, including LDPE, HDPE, PET, PP, and PS. Ten bacterial isolates were obtained and screened for their ability to grow on microplastic-supplemented mineral salt medium over 20 days. Three isolates (BPM 5, BPM 9, and BPM 10) demonstrated robust growth, with biochemical tests confirming catalase activity critical for managing oxidative stress during plastic degradation. Molecular identification via 16S rRNA gene sequencing revealed the isolates as Enterobacter sp. and Brevibacillus parabrevis, both known for their biodegradation capacities. The findings underscore the diverse metabolic pathways these bacteria employ to utilize microplastics as carbon sources. This research highlights the promise of mangrove sediment bacteria as eco-friendly bioremediation agents to mitigate microplastic pollution in coastal environments. Further studies are required to optimize degradation conditions and elucidate enzymatic mechanisms to enhance practical applications.
Co-Authors Agnes Adela Br Ginting Ayu Putri Ningsih Cindy Suci Muliandhira HANDAYANI, DINA Larasati Arum Utami Mhd Yusuf Nasution Palsan Sannasi Pulungan, Ahmad Shafwan S Sahilah Abdul Mutalib Tessa Togatorop