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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Florea Jurnal Penelitian Pendidikan IPA (JPPIPA) Martabe : Jurnal Pengabdian Kepada Masyarakat Sanitasi: Jurnal Kesehatan Lingkungan
Marlinda Nilan Sari Rangkuti
Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences

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PERTUMBUHAN KULTUR KONSORTIUM DALAM MINYAK SELAMA BIODEGRADASI DI BAWAH KONDISI AEROB Marlinda Nilan Sari Rangkuti; Palsan Sannasi; Sahilah Abdul Mutalib
JBIO: jurnal biosains (the journal of biosciences) Vol 4, No 1 (2018): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v4i1.9783

Abstract

This study reported the ability of the Consortium Culture (5% v/v) was grown in minimum salts medium (MSM) with the addition of 1% (v / v) crude oil, at different incubation times (0, 2, 4, 6 and 8 weeks). The Consortium Culture (CC) can live and use crude oil as the sole source of carbon and energy. The analysis result shows that the highest percentage of biodegradation is at the second week with the percentage of hydrocarbon residue of 42% and it has been used up in the eighth week. These results indicate that the consortium culture can be utilized as a biodegradation agent and this phenomenon can used to enhance the bioremediation ability of the petroleum hydrocarbon polluted environment.
TEST OF ANTIBACTERIAL ACTIVITY OF THE ETHANOL EXTRACT OF PULUTAN LEAVES (Urena lobata L.) AGAINST Escherichia coli Mhd Yusuf Nasution; Marlinda Nilan Sari Rangkuti; Dina Handayani; Tessa Togatorop; Agnes Adela Br Ginting; Ahmad Shafwan S Pulungan
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 2 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i2.48764

Abstract

ABSTRAK  Tessa Togatorop, NIM 4193520022, Uji Aktivitas Ekstrak Etanol Daun Pulutan Daun Pulutan (Urena lobata L.) Terhadap Bakteri Escherichia coli Dan Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder, uji aktivitas antibakteri ekstrak etanol daun pulutan (Urena lobata L.) dan untuk mengetahui uji Konsentrasi Hambat Minimum (KHM) dan uji Kosentrasi Bunuh Minimum (KBM) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Uji skrining fitokimia dilakukan dengan beberpa pereaksi yang sesuai. Uji aktivitas antibakteri menggunakan metode difusi cakram terhadap bakteri Escherichia coli dan Staphylococcus aureus. Uji KHM dan KBM dilakukan dengan menghitung selisih nilai absorbansi setelah inkubasi dan sebelum inkubasi yang dapat menghambat dan membunuh bakteri uji. Hasil skrining fitokimia esktrak etanol daun pulutan mengandung; flavonoid, saponin, tanin, dan steroid/terpenoid. Hasil uji aktivitas antibakteri dengan metode difusi cakram ekstrak etanol daun pulutan 15%, 25%, 35%, 45%, 55% dan 65% terhadap bakteri Escherichia coli berturut-turut adalah 4,0625 mm, 4,2 mm, 4,85 mm, 3,075 mm, 4,5875 mm, dan 4,3 mm sedangkan pada bakteri Staphylococcus aureus berturut-turut adalah 2,5625 mm, 3,05 mm, 3,35 mm, 3,55 mm, 3,175 mm, dan 4,5 mm. Hasil penelitian menunjukkan ekstrak etanol daun pulutan memiliki aktivitas antibakteri terhadap bakteri Escherhicia coli dan Staphylococcus aureus dalam kategori yang lemah. Uji KHM pada bakteri Escherichia coli adalah konsentrasi 65% dan pada bakteri Staphylococcus aureus adalah konsentrasi 55%. Uji KBM pada bakteri Escherichia coli dan Staphylococcus aureus tidak didapat pada setiap perlakuan konsentrasi. Kata Kunci : Pulutan (Urena lobata L.), Antibakteri, Konsentrasi Hambat Kosentrasi Bunuh Minimum
Potential Microplastic-Degrading Bacteria from Mangrove Sediment in The Paluh Getah Area, Percut Sei Tuan District Ahmad Shafwan S. Pulungan; Larasati Arum Utami; Marlinda Nilan Sari Rangkuti; Ayu Putri Ningsih; Cindy Suci Muliandhira
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12647

Abstract

Microplastic pollution poses a severe threat to marine ecosystems due to its persistence and toxicity, necessitating innovative biodegradation strategies. This study explored the potential of bacteria isolated from mangrove sediments of the Paluh Getah mangrove forest, North Sumatra, to degrade common microplastics, including LDPE, HDPE, PET, PP, and PS. Ten bacterial isolates were obtained and screened for their ability to grow on microplastic-supplemented mineral salt medium over 20 days. Three isolates (BPM 5, BPM 9, and BPM 10) demonstrated robust growth, with biochemical tests confirming catalase activity critical for managing oxidative stress during plastic degradation. Molecular identification via 16S rRNA gene sequencing revealed the isolates as Enterobacter sp. and Brevibacillus parabrevis, both known for their biodegradation capacities. The findings underscore the diverse metabolic pathways these bacteria employ to utilize microplastics as carbon sources. This research highlights the promise of mangrove sediment bacteria as eco-friendly bioremediation agents to mitigate microplastic pollution in coastal environments. Further studies are required to optimize degradation conditions and elucidate enzymatic mechanisms to enhance practical applications.
Analysis of Student Learning Difficulties in Human Movement System Material for Class XI at SMA Negeri 1 Silimakuta. Febri Ulina Feronika Girsang; Marlinda Nilan Sari Rangkuti
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 1 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i1.23397

Abstract

This study aims to determine students' learning difficulties and the factors causing these difficulties in learning the human movement system material for Class XI at SMA Negeri 1 Silimakuta. The research population was all Class XI students who studied biology. The sample was taken using Purposive Sampling, and the method used was descriptive quantitative. The instruments used in this study were essay tests and questionnaires. Data were analyzed using a percentage technique. The results showed that:(1) The percentage of student learning difficulties at the cognitive level for knowledge (C1) questions was 50.69%, comprehension (C2) was 63.75%, application (C3) was 58.84%, analysis (C4) was 57.08%, evaluation (C5) was 67.43%, and creation (C6) was 74.44%.(2) Furthermore, the first indicator was 62,96%, the second indicator was 59,72%, the third indicator was 61,06 %, and the fourth indicator was 61.34%. (3) The factors influencing student learning difficulties were examined from both internal and external aspects. The internal factors included: interest at 76.59%, motivation at 79.99%, intelligence at 73.04%, and attention at 74.63%. The external factors included: books at 72.61%, media at 70.97%, teacher's teaching methods at 76.49%, and facilities and infrastructure at 73.83%.
PEMANFAATAN ECENG GONDOK SEBAGAI PAKAN ALTERNATIF UNGGAS MELALUI REKAYASA NUTRISI DAN MEKANISASI PRODUKSI UNTUK PENGENDALIAN DEGRADASI LINGKUNGAN Tri Harsono; Marlinda Nilan Sari Rangkuti; Suci Rahmawati; Adriana Y.D. Lbn Gaol; Rizal Mukra; Widya Arwita; Mustaqim Mustaqim; Widia Ningsih; Imelda Nababan
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 9, No 7 (2026): MARTABE : JURNAL PENGABDIAN MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v9i7.2849-2856

Abstract

This activity was carried out in Medan Marelan, with the Melati Partner Group. The main problems were the dependence on commercial feed reaching 80% of production costs and the high population of water hyacinth covering 10% of the water surface, causing flooding and ecosystem disruption. The objective of this activity was to provide an alternative through the utilization of water hyacinth as chicken feed. The activity was conducted from April to June 2026 through four stages, namely: socialization to increase partner awareness about the potential of water hyacinth; training and technology application which included structured harvesting techniques, the use of a chopper machine, a feed pellet machine, and the production of fermented feed with a formulation of 10 kg of rice bran, 10 kg of corn, 10 kg of concentrate, 10% water hyacinth (equivalent to 3.33 kg), and sufficient EM4 solution; followed by intensive mentoring to ensure feed processing skills were implemented independently; and monitoring. The results showed that 10% fermented water hyacinth feed resulted in a cost per kilogram of feed of Rp5,366, which is cheaper than non-fermented feed at Rp5,912/kg, enabling partners to save costs of Rp18,270 per 33.33 kg of feed, or approximately 9.2% more efficient. Furthermore, an increase in chicken body weight of 0.4 kg indicated that chicken performance was maintained and not significantly different from 100% commercial feed. This activity also supports the Sustainable Development Goals (SDGs). The utilization of 10% fermented water hyacinth provides a highly beneficial dual impact
Analisis mikrobiologis kualitas udara di lingkungan Universitas Negeri Medan Lydia Siburian; Fatiha Salma Siregar; Putri Khairani; Gracelda E. Pardede; Rani Simanjuntak; Najwa Athifah; Marlinda Nilan Sari Rangkuti; Adelia Febriyossa
Sanitasi: Jurnal Kesehatan Lingkungan Vol. 19 No. 2 (2026): Article in Progress
Publisher : Poltekkes Kemenkes Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/sanitasi.v19i2.3284

Abstract

Kualitas mikrobiologi udara merupakan salah satu indikator penting dalam penilaian kesehatan lingkungan karena keberadaan mikroorganisme di udara berpotensi mempengaruhi kesehatan manusia. Penelitian ini dilakukan untuk menganalisis kualitas mikrobiologi udara berdasarkan kepadatan dan karakteristik bakteri menggunakan metode passive air sampling. Pengambilan sampel udara dilakukan pada tiga lokasi dengan tingkat aktivitas yang berbeda menggunakan media Nutrient Agar (NA). Sampel yang diperoleh diinkubasi pada suhu 37°C selama 24–48 jam, kemudian dilakukan perhitungan jumlah koloni bakteri dalam satuan Colony Forming Unit (CFU), pengamatan morfologi koloni, pewarnaan Gram, serta uji biokimia yang meliputi uji katalase, Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), dan Simmons Citrate. Hasil penelitian menunjukkan bahwa kepadatan mikroorganisme udara tertinggi ditemukan pada lokasi Gerbang 1 sebesar 3,07 × 10³ CFU/m³, sedangkan Gerbang 2 dan Gerbang 4 masing-masing sebesar 1,67 × 10³ CFU/m³ dan 1,62 × 10³ CFU/m³. Karakterisasi bakteri menunjukkan adanya variasi morfologi koloni serta dominasi bakteri Gram positif berbentuk basil dan beberapa isolat Gram negatif berbentuk kokobasil. Hasil uji biokimia mengindikasikan adanya keragaman karakter fisiologis antar isolat bakteri. Penelitian ini menyimpulkan bahwa kualitas mikrobiologi udara pada lokasi pengamatan menunjukkan variasi kepadatan dan karakteristik bakteri yang dipengaruhi oleh kondisi lingkungan dan aktivitas di sekitar lokasi sampling.   Abstract: Air microbiological quality is one of the key indicators in environmental health assessments, as the presence of microorganisms in the air has the potential to affect human health. This study was conducted to analyse air microbiological quality based on bacterial density and characteristics using the passive air sampling method. Air sampling was carried out at three locations with different activity levels using Nutrient Agar (NA) as the culture medium. The samples obtained were incubated at 37°C for 24–48 hours, followed by the counting of bacterial colonies in Colony Forming Units (CFU), observation of colony morphology, Gram staining, and biochemical tests including the catalase test, Triple Sugar Iron Agar (TSIA), Sulphide Indole Motility (SIM), and Simmons Citrate tests. The results of the study showed that the highest density of airborne microorganisms was found at Gate 1, at 3.07 × 10³ CFU/m³, whilst Gate 2 and Gate 4 recorded 1.67 × 10³ CFU/m³ and 1.62 × 10³ CFU/m³ respectively. Characterisation of the bacteria revealed variations in colony morphology, with a predominance of Gram-positive bacilli and several Gram-negative coccobacilli isolates. Biochemical test results indicated diversity in the physiological characteristics among the bacterial isolates. This study concluded that the microbiological quality of the air at the observation sites showed variations in bacterial density and characteristics influenced by environmental conditions and activity.
Co-Authors Adelia Febriyossa Adriana Y.D. Lbn Gaol Agnes Adela Br Ginting Ayu Putri Ningsih Cindy Suci Muliandhira Fatiha Salma Siregar Febri Ulina Feronika Girsang Gracelda E. Pardede HANDAYANI, DINA Imelda Nababan Larasati Arum Utami Lydia Siburian Mhd Yusuf Nasution Mustaqim Mustaqim Najwa Athifah Palsan Sannasi Pulungan, Ahmad Shafwan S Putri Khairani Rani Simanjuntak Rizal Mukra Sahilah Abdul Mutalib Suci Rahmawati Tessa Togatorop Tri Harsono Widia Ningsih Widya Arwita