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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Eksakta : Berkala Ilmiah Bidang MIPA AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Biogenerasi Didaktik : Jurnal Ilmiah PGSD STKIP Subang Jurnal Pendidikan, Sains, Geologi, dan Geofisika (GeoScienceEd Journal) BIOCHEPHY: Journal of Science Education EDU-BIO : Jurnal Pendidikan Biologi Journal of Innovative and Creativity
Larasati Arum Utami
Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Neuroscience Physics Social Sciences Other

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The Effect of Ethanol Solvent Concentration on Antimicrobial Activities The Extract of Andalas Endophytic Bacteria (Morus Macroura Miq.) Fermentation Product Larasati Arum Utami; Dwi Hilda Putri
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 21 No. 1 (2020): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.062 KB) | DOI: 10.24036/eksakta/vol21-iss1/210

Abstract

Anti-Biotic resistance is a health problem globally. It is able to overcome with a new anti-microbial compound that can be produced from Andalas endophytic bacteria. This compound can be obtained through the fermentation process. In order to separate this active-compound, it is needed to use the extraction method. In this method, the solvent is functioned as the extractor. One of the solvent which is commonly used is ethanol. This research is aimed to know the effect of ethanol concentration toward antibacterial activity from extracted bacterial fermentation products of Andalas endophytic bacteria isolate JDT 1B. The fermented products are extracted by using the maceration method. The concentrations are 100%, 80%, 70%. A test of anti-microbial activity is used disk diffusion method. The extracted concentrations tested for each solvent are 50%, 25%, 12,5%, and 6.25%. Anti-bacterial activity is analyzed by using factorial design. The factorial result showed there is no significant contrast between ethanol concentration mentioned toward anti-bacterial activity from extracted bacterial fermentation products of Andalas endophytic bacteria Isolate JDT 1B. The concentration of extracted fermentation product using 70% ethanol has the same inhibition zone as control is 6,25%.
Identification of Microbial Contamination in Street Food Around Universitas Negeri Medan Larasati Arum Utami; Idramsa Idramsa; Artika Rindiani; Najwa Liliana; Nita Maya Sari Pane; Santaro Marito Nadeak
Journal of Innovative and Creativity Vol. 5 No. 2 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i2.2647

Abstract

Street food remains a popular choice for students due to its affordability and convenience. Its rapid preparation and service contribute to its ongoing popularity. The university, including Universitas Negeri Medan, is often surrounded by numerous street vendors offering a diverse range of foods, predominantly made from meat and flour. However, the open exposure of these foods and insufficient sanitation practices pose a heightened risk of contamination by harmful microorganisms. This study examines bacterial contamination in processed meat-based street foods sold near Universitas Negeri Medan, with a focus on items such as meatballs, grilled meatballs, and batagor. Microbiological examination involved isolating bacteria using selective and differential culture media, particularly Eosin Methylene Blue Agar (EMBA). Subsequent identification was carried out using Gram staining to determine cell morphology and SIM (Sulfide Indole Motility) medium for motility assessment in the microbiology lab at Universitas Negeri Medan. Findings revealed contamination predominantly in grilled meatballs, with bacteria belonging to the Enterobacteriaceae family detected. These isolates were characterized as Gram-negative, rod-shaped, and motile microorganisms. The presence of such bacteria raises serious concerns about food safety. It emphasizes the need for improved hygienic practices and rigorous food safety control measures in street food vending areas around the university. Implementing these measures is essential to safeguard student health and maintain the standard of street food quality on campus.
Potential Microplastic-Degrading Bacteria from Mangrove Sediment in The Paluh Getah Area, Percut Sei Tuan District Ahmad Shafwan S. Pulungan; Larasati Arum Utami; Marlinda Nilan Sari Rangkuti; Ayu Putri Ningsih; Cindy Suci Muliandhira
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12647

Abstract

Microplastic pollution poses a severe threat to marine ecosystems due to its persistence and toxicity, necessitating innovative biodegradation strategies. This study explored the potential of bacteria isolated from mangrove sediments of the Paluh Getah mangrove forest, North Sumatra, to degrade common microplastics, including LDPE, HDPE, PET, PP, and PS. Ten bacterial isolates were obtained and screened for their ability to grow on microplastic-supplemented mineral salt medium over 20 days. Three isolates (BPM 5, BPM 9, and BPM 10) demonstrated robust growth, with biochemical tests confirming catalase activity critical for managing oxidative stress during plastic degradation. Molecular identification via 16S rRNA gene sequencing revealed the isolates as Enterobacter sp. and Brevibacillus parabrevis, both known for their biodegradation capacities. The findings underscore the diverse metabolic pathways these bacteria employ to utilize microplastics as carbon sources. This research highlights the promise of mangrove sediment bacteria as eco-friendly bioremediation agents to mitigate microplastic pollution in coastal environments. Further studies are required to optimize degradation conditions and elucidate enzymatic mechanisms to enhance practical applications.
Analisis Fase Mitosis pada Akar Bawang Merah (Allium cepa) yang Direndam dalam Air Beras, Air Kelapa Muda, dan Air Biasa Damai Agatha Tarihoran; Marsedina Berutu; Winda Resta Laoli; Putu Azkia Shafrina; Larasati Arum Utami
Jurnal Pendidikan, Sains, Geologi, dan Geofisika (GeoScienceEd Journal) Vol. 7 No. 2 (2026): May
Publisher : Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/goescienceed.v7i2.1964

Abstract

Mitosis cell division is an important process in plant growth and occurs actively in meristematic tissues, one of which is the root tip of shallots (Allium cepa). Mitotic activity can be influenced by the growing medium used, especially media containing nutrients and growth regulators. This study aimed to examine the process of mitotic cell division in shallot root tips soaked in three different media, namely plain water, rice washing water, and young coconut water. The research was conducted using an experimental method with a descriptive approach. Shallots were soaked for approximately 48 hours until roots were formed, then the root tips were prepared using the squash method and observed under a light microscope. The observed parameters included the stages of mitosis and root growth. The results showed that the plain water medium only exhibited prophase and telophase stages with low mitotic activity. The rice washing water medium showed telophase and cytokinesis stages with moderate mitotic activity. Meanwhile, the young coconut water medium exhibited more complete mitotic stages, namely prophase, telophase, and cytokinesis, and produced the most optimal root growth. These findings indicate that the type of soaking medium affects mitotic activity in shallot roots, with young coconut water being the most effective medium in supporting cell division.
Identifikasi Pengaruh Jenis Air Perendaman terhadap Fase Mitosis dan Pertumbuhan Akar Bawang Merah (Allium cepa L.) dengan Menggunakan Air Biasa, Air AC, dan Air Hujan Laura Capenesi Simanjuntak; Dhea Miranda Tambunan; Immanuel Siregar; Andrian Raja Noel Tampubolon; Larasati Arum Utami
Jurnal Pendidikan, Sains, Geologi, dan Geofisika (GeoScienceEd Journal) Vol. 7 No. 2 (2026): May
Publisher : Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/goescienceed.v7i2.1988

Abstract

This study aimed to determine the effect of using tap water, air conditioner (AC) water, and rainwater on root growth and mitotic phases in shallot roots (Allium cepa L.). The study employed an experimental method with a quantitative descriptive approach and a Completely Randomized Design (CRD). Shallot bulbs were immersed for 48 hours in three different types of water: tap water, AC water, and rainwater. Observations were conducted on root length and mitotic phases through microscopic examination using the squash method. Data were collected through direct observation and documentation of the observation results, then analyzed descriptively by calculating the average root length and the frequency of mitotic phases observed. The results showed that all treatments were able to support root growth and mitotic activity in the root meristem tissue of shallots. The best root growth was observed in the tap water and rainwater treatments, with an average root length of 1.8 cm, while the AC water treatment produced an average root length of 1.7 cm. Microscopic observations revealed that root cells in all treatments were predominantly in the telophase stage, characterized by the reformation of the nuclear membrane and the initiation of cytokinesis. Differences in mineral content among the water types were presumed to influence cell division activity and root cell elongation. Therefore, the type of soaking water affected root growth, although all treatments were capable of supporting the mitotic process in Allium cepa L.
Utilisation of Turmeric (Curcuma longa L.) Extract as a Natural Indicator for Borax Detection in Food Products Dea Afnun Harahap; Browin Christmas Ginting; Nadia Pentatriana Purba; Grace Yosepha Sinaga; Farel Vandano Hiskia Sebayang; Larasati Arum Utami; Nurbaity Situmorang
BIOCHEPHY: Journal of Science Education Vol. 6 No. 1 (2026)
Publisher : MO.RI Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52562/biochephy.v6i1.1969

Abstract

The widespread misuse of sodium tetraborate among informal food producers highlights the urgent need for eco-friendly, independent food screening methods. This study aimed to validate the effectiveness of turmeric rhizome (Curcuma longa L.) as an initial screening instrument for borax in high-moisture food clusters. An experimental method was applied through direct visual contact testing of reactive curcumin extract against seven types of wet domestic food samples. Colorimetric test results showed that deep orange boron cyano curcumin (rosocyanine) complex formation was detected exclusively and positively in yellow noodle samples. Six other products chicken meatballs, fish balls, pempek, white tofu, vermicelli, and crackers were confirmed negative. It is concluded that the alkaline-induced structural degradation triggered by this local natural indicator demonstrates precise reactive sensitivity, is economically viable, and is applicable as a communal preventive solution for enhancing household food safety literacy.
Effectiveness of Purple Cabbage Flower (Brassica oleracea var. capitata f. rubra) Extract as a Natural Indicator for Borax Identification in Food Products Fauziah Indira Siregar; Rahel Nainggolan; Nayla Azizah; Teresia Vredemika Sidauruk; Miquel Kaban; Larasati Arum Utami; Nurbaity Situmorang
BIOCHEPHY: Journal of Science Education Vol. 6 No. 1 (2026)
Publisher : MO.RI Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52562/biochephy.v6i1.1970

Abstract

The misuse of borax as a preservative in school snacks poses a serious threat to food safety, necessitating practical and accessible solutions for the general public. This study aimed to evaluate the effectiveness of purple cabbage (Brassica oleracea var. capitata f. rubra) extract as a natural indicator for the qualitative detection of borax. Laboratory experiments were conducted at the Biology Laboratory of Universitas Negeri Medan using the maceration method with water as the solvent to extract anthocyanin pigments. Food samples tested included meatballs, tofu, yellow noodles, and crackers. The results showed that purple cabbage extract successfully detected borax in yellow noodle samples through a colour change from purple to bluish-green, caused by the deprotonation of anthocyanins under alkaline conditions. These findings demonstrate that purple cabbage extract is a valid, economical, practical, and environmentally friendly screening tool to support public food safety monitoring.
Analisis Perbandingan Ekstrak Kulit Buah Naga (Hylocereus polyrhizus) dan Kunyit (Curcuma longa L.) Sebagai Indikator Identifikasi Boraks Pada Makanan Claudia Nainggolan; Eka Setiawan; Emon Sejahtera Zendrato; Grecia Trinatal Simbolon; Kania Restya Diva; Larasati Arum Utami; Rejeki Sihite
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/sbjm5j61

Abstract

This study aimed to analyze and compare the effectiveness of red dragon fruit peel (Hylocereus polyrhizus) extract and turmeric (Curcuma longa L.) extract as natural indicators for detecting borax in food samples. The research used a laboratory experimental method with a qualitative descriptive approach. Food samples tested included sausages, ketupat, lontong, white tofu, crackers, siomay, meatballs, cilok, pempek, nuggets, white noodles, and yellow noodles. The testing process was conducted by dripping dragon fruit peel extract and turmeric extract onto each sample and observing the resulting color changes. The results showed that most samples tested negative for borax, while meatballs and yellow noodles indicated positive results. The color change in turmeric to reddish-brown occurred due to the reaction between curcumin and borax, while dragon fruit peel extract changed color because anthocyanin pigments are sensitive to pH changes. The findings indicate that both natural materials can be used as simple, inexpensive, safe, and effective natural indicators for the qualitative preliminary detection of borax in food. However, further quantitative testing is needed to obtain more accurate results.
PEMBELAJARAN INTERDISIPLINER: EDUKASI HOLISTIK UNTUK MENINGKATKAN LITERASI SAINS DAN LITERASI BACA ANAK SD DI DESA BINJAI Tariza Fairuz; Larasati Arum Utami; Vera Marisa
Amaliah: Jurnal Pengabdian Kepada Masyarakat Vol 9 No 1 (2025): Amaliah Jurnal: Pengabdian kepada Masyarakat
Publisher : LPPI UMN AL WASHLIYAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/ajpkm.v9i1.4271

Abstract

Science literacy and reading literacy are two key competencies that not only support academic success but also prepare children to face global challenges in the 21st century. Science literacy includes the ability to understand scientific concepts, think critically, and apply that knowledge in everyday life. Meanwhile, reading literacy involves the ability to understand, analyze, and use information from written texts. Science literacy and reading literacy of elementary school children in Binjai Village are still low and need holistic education with interdisciplinary learning programs. This community service program aims to improve science literacy and reading literacy of elementary school children in Binjai Village through interdisciplinary learning. The implementation method in this community service activity is in accordance with the partner's problem, through training in interdisciplinary learning that includes aspects of science literacy and reading literacy in English and Arabic adapted from quantitative research methods with a one-class pretest-posttest design. This service was carried out in Binjai Village, Tebing Syahbandar District, Serdang Bedagai Regency. There were 30 elementary school children who participated in this activity. The results of this activity show an increase in science literacy and reading literacy of elementary school children in Binjai village.
Bunga Telang (Clitoria ternatea L.) sebagai Alternatif Indikator Alami dalam Pengujian Kandungan Boraks Hans Filbert Nadeak; Aida Turrohma Hasibuan; Helen Cindytha Pasaribu; Rahel Oriza Girsang; Larasati Arum Utami; Nurbaity Situmorang
EDU-BIO: Jurnal Pendidikan Biologi Vol. 9 No. 2 (2026): EDU-BIO: Jurnal Pendidikan Biologi
Publisher : Prodi Tadris Biologi Fakultas Tarbiyah dan Keguruan Universitas Islam Negeri Sulthan Thaha Saifuddin Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/edubio.v9i2.223

Abstract

Keamanan pangan merupakan isu krusial yang berkaitan langsung dengan kesehatan masyarakat, namun penyalahgunaan bahan tambahan pangan berbahaya seperti boraks masih marak ditemukan pada produk pangan olahan. Penelitian ini bertujuan untuk menguji kemampuan ekstrak bunga telang (Clitoria ternatea L.) sebagai indikator alami untuk mendeteksi kandungan boraks secara kualitatif pada berbagai sampel makanan. Metode penelitian yang digunakan adalah eksperimen laboratorium dengan pendekatan deskriptif kualitatif terhadap tujuh sampel makanan, yaitu bakso ayam, bakso ikan, pempek, tahu putih, bihun, mie kuning, dan kerupuk ikan. Hasil penelitian menunjukkan bahwa ekstrak bunga telang yang mengandung pigmen antosianin sensitif terhadap perubahan pH, di mana kondisi basa akibat keberadaan boraks akan memicu perubahan warna dari biru keunguan menjadi hijau. Dari seluruh sampel yang diuji, hanya sampel mie kuning yang menunjukkan perubahan warna menjadi hijau, yang mengindikasikan adanya kandungan boraks. Sementara itu, enam sampel lainnya menunjukkan hasil negatif. Simpulan dari penelitian ini adalah ekstrak bunga telang efektif digunakan sebagai instrumen skrining awal yang sederhana, aman, dan ekonomis untuk mengidentifikasi kontaminasi boraks pada makanan, serta berpotensi dikembangkan sebagai media edukasi keamanan pangan bagi masyarakat.
Co-Authors Aida Turrohma Hasibuan Andrian Raja Noel Tampubolon Annisa Putri Ginting Aprilla Rahel Yolanda Sinaga Artika Rindiani Ayu Putri Ningsih Boi David Nainggolan Browin Christmas Ginting Cindy Suci Muliandhira Claudia Nainggolan Damai Agatha Tarihoran Dea Afnun Harahap Dea Alfioni Br. Tarigan Dhea Miranda Tambunan Dita Salsabila Dwi Hilda Putri Eka Setiawan Emon Sejahtera Zendrato Ezra Sijabat Fairuz, Tariza Farel Vandano Hiskia Sebayang Fauziah Indira Siregar Gloria Caroline Siahaan Grace Dearni Batubara Grace Yosepha Sinaga Grecia Trinatal Simbolon Hans Filbert Nadeak Helen Cindytha Pasaribu Idramsa Idramsa Immanuel Siregar Intan Cahya Lestari Jemima Fevriera Bangun Kania Restya Diva Krisdayanti Febyola Angelina Ambarita Laura Capenesi Simanjuntak Marlinda Nilan Sari Rangkuti Marsedina Berutu Miquel Kaban Muhammad Hafiz Siddiq Muhammad Rio Adinatha Nabil Arkan Harahap Nadia Pentatriana Purba Najwa Liliana Nayla Azizah Nita Maya Sari Pane Nurbaity Situmorang Pulungan, Ahmad Shafwan S Putu Azkia Shafrina Rahel Nainggolan Rahel Oriza Girsang Rejeki Sihite Salsabilla Salsabilla Santaro Marito Nadeak Syahilla Az Zahra Teresia Vredemika Sidauruk Tri Wahyuni Vera Marisa Winda Resta Laoli Yohana Putri Girsang Yulan Marcelia Banjarnahor