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The Effect of Ethanol Solvent Concentration on Antimicrobial Activities The Extract of Andalas Endophytic Bacteria (Morus Macroura Miq.) Fermentation Product Larasati Arum Utami; Dwi Hilda Putri
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 21 No. 1 (2020): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.062 KB) | DOI: 10.24036/eksakta/vol21-iss1/210

Abstract

Anti-Biotic resistance is a health problem globally. It is able to overcome with a new anti-microbial compound that can be produced from Andalas endophytic bacteria. This compound can be obtained through the fermentation process. In order to separate this active-compound, it is needed to use the extraction method. In this method, the solvent is functioned as the extractor. One of the solvent which is commonly used is ethanol. This research is aimed to know the effect of ethanol concentration toward antibacterial activity from extracted bacterial fermentation products of Andalas endophytic bacteria isolate JDT 1B. The fermented products are extracted by using the maceration method. The concentrations are 100%, 80%, 70%. A test of anti-microbial activity is used disk diffusion method. The extracted concentrations tested for each solvent are 50%, 25%, 12,5%, and 6.25%. Anti-bacterial activity is analyzed by using factorial design. The factorial result showed there is no significant contrast between ethanol concentration mentioned toward anti-bacterial activity from extracted bacterial fermentation products of Andalas endophytic bacteria Isolate JDT 1B. The concentration of extracted fermentation product using 70% ethanol has the same inhibition zone as control is 6,25%.
PEMBELAJARAN INTERDISIPLINER: EDUKASI HOLISTIK UNTUK MENINGKATKAN LITERASI SAINS DAN LITERASI BACA ANAK SD DI DESA BINJAI Fairuz, Tariza; Utami, Larasati Arum; Marisa, Vera
Amaliah: Jurnal Pengabdian Kepada Masyarakat Vol 9 No 1 (2025): Amaliah Jurnal: Pengabdian kepada Masyarakat
Publisher : LPPI UMN AL WASHLIYAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/ajpkm.v9i1.4271

Abstract

Literasi sains dan literasi baca merupakan dua kompetensi kunci yang tidak hanya mendukung keberhasilan akademis, tetapi juga mempersiapkan anak-anak untuk menghadapi tantangan global di abad ke-21. Literasi sains mencakup kemampuan untuk memahami konsep-konsep sains, berpikir kritis, dan menerapkan pengetahuan tersebut dalam kehidupan sehari-hari. Sementara itu, literasi baca melibatkan kemampuan untuk memahami, menganalisis, dan menggunakan informasi dari teks tertulis. Literasi sains dan literasi baca anak-anak SD di Desa Binjai masih rendah dan perlu edukasi yang holistik dengan program pembelajaran interdisipliner. Program pengabdian kepada masyarakat ini bertujuan untuk meningkatkan literasi sains dan literasi baca anak SD di desa Binjai melalui pembelajaran interdisipliner. Metode pelaksanaan dalam kegiatan pengabdian masyarakat ini sesuai dengan masalah mitra, melalui pelatihan dalam pembelajaran interdisipliner yang memuat aspek literasi sains dan literasi baca yang diadaptasi dari metode penelitian kuantitatif dengan desain pretes-postes satu kelas. Pengabdian ini dilaksanakan di desa Binjai kecamatan Tebing Syahbandar kabupaten Serdang Bedagai. Anak-anak SD yang mengikuti kegiatan ini berjumlah 30 orang. Hasil kegiatan ini menunjukkan ada peningkatan literasi sains dan literasi baca anak-anak SD di desa Binjai.
Identification of Microbial Contamination in Street Food Around Universitas Negeri Medan Utami, Larasati Arum; Idramsa, Idramsa; Rindiani, Artika; Liliana, Najwa; Pane, Nita Maya Sari; Nadeak, Santaro Marito
Journal of Innovative and Creativity Vol. 5 No. 2 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i2.2647

Abstract

Street food remains a popular choice for students due to its affordability and convenience. Its rapid preparation and service contribute to its ongoing popularity. The university, including Universitas Negeri Medan, is often surrounded by numerous street vendors offering a diverse range of foods, predominantly made from meat and flour. However, the open exposure of these foods and insufficient sanitation practices pose a heightened risk of contamination by harmful microorganisms. This study examines bacterial contamination in processed meat-based street foods sold near Universitas Negeri Medan, with a focus on items such as meatballs, grilled meatballs, and batagor. Microbiological examination involved isolating bacteria using selective and differential culture media, particularly Eosin Methylene Blue Agar (EMBA). Subsequent identification was carried out using Gram staining to determine cell morphology and SIM (Sulfide Indole Motility) medium for motility assessment in the microbiology lab at Universitas Negeri Medan. Findings revealed contamination predominantly in grilled meatballs, with bacteria belonging to the Enterobacteriaceae family detected. These isolates were characterized as Gram-negative, rod-shaped, and motile microorganisms. The presence of such bacteria raises serious concerns about food safety. It emphasizes the need for improved hygienic practices and rigorous food safety control measures in street food vending areas around the university. Implementing these measures is essential to safeguard student health and maintain the standard of street food quality on campus.
Potential Microplastic-Degrading Bacteria from Mangrove Sediment in The Paluh Getah Area, Percut Sei Tuan District Pulungan, Ahmad Shafwan S.; Utami, Larasati Arum; Rangkuti, Marlinda Nilan Sari; Ningsih, Ayu Putri; Muliandhira, Cindy Suci
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12647

Abstract

Microplastic pollution poses a severe threat to marine ecosystems due to its persistence and toxicity, necessitating innovative biodegradation strategies. This study explored the potential of bacteria isolated from mangrove sediments of the Paluh Getah mangrove forest, North Sumatra, to degrade common microplastics, including LDPE, HDPE, PET, PP, and PS. Ten bacterial isolates were obtained and screened for their ability to grow on microplastic-supplemented mineral salt medium over 20 days. Three isolates (BPM 5, BPM 9, and BPM 10) demonstrated robust growth, with biochemical tests confirming catalase activity critical for managing oxidative stress during plastic degradation. Molecular identification via 16S rRNA gene sequencing revealed the isolates as Enterobacter sp. and Brevibacillus parabrevis, both known for their biodegradation capacities. The findings underscore the diverse metabolic pathways these bacteria employ to utilize microplastics as carbon sources. This research highlights the promise of mangrove sediment bacteria as eco-friendly bioremediation agents to mitigate microplastic pollution in coastal environments. Further studies are required to optimize degradation conditions and elucidate enzymatic mechanisms to enhance practical applications.