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All Journal Best Journal (Biology Education, Sains and Technology) Rumphius Pattimura Biological Journal
Nengah Dwianita Kuswytasari
Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health

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Isolasi, Seleksi, Identifikasi Jamur Pelapuk Kayu Berpotensi Produksi Lipase Fitriani, Aprilia Dyah; Prasetyo, Endry Nugroho; Kuswytasari, Nengah Dwianita
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10448

Abstract

Lipase dapat dihasilkan dari makhluk hidup baik itu hewan, tumbuhan maupun mikroorganisme seperti bakteri, dan jamur. Salah satu aktivitas lipolitik berasal dari jamur pelapuk kayu di Pulau Poteran, Dusun Sakolaan, Jawa Timur. Isolasi Jamur Pelapuk Kayu diambil menggunakan metode hand collecting dan diinokulasikan ke media agar. Skrining jamur pelapuk kayu sebanyak 6 isolat menggunakan media Czapek-dox Agar dan indikator Rhodamine-b. Jamur Isolat M12 berpotensi memiliki aktivitas lipolitik dengan menghasilkan warna pendar oranye dibawah sinar UV kemudian diidentifikasi secara makroskopis dan mikroskopis yang merupakan Jamur Trametes sp. Pengamatan kurva pertumbuhan menimbang berat basah miselium selama 14 hari untuk mengetahui fase pertumbuhan dan deteksi aktivitas lipase menggunakan substrat p-Nitrophenol. Hasil kurva pertumbuhan produksi lipase menggunakan metode submerged fermentation yang tertinggi terdapat pada inkubasi hari ke-7 dengan aktivitas crude lipase sebesar 229 U/ml.
Ethnopharmacy and Traditional Knowledge Study with a Family Use Value Approach in Sumberbrantas Village, Bumiaji District, Batu City, East Java Rosyid Ridho, Muhammad Ainur; Trisnawati, Indah; Nurhidayati, Tutik; Purwani, Kristanti Indah; Saptarini, Dian; Kuswytasari, Nengah Dwianita
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p088-100

Abstract

Indonesia is recognized as one of the world’s most biodiverse countries, with an estimated 17% of global plant and animal species found within its borders. This biodiversity supports a wealth of medicinal plants that have been traditionally utilized by local communities. However, rapid modernization has led to a decline in the transmission of ethnobotanical knowledge. This study investigates the ethnopharmacological practices in Sumberbrantas Village, Bumiaji District, Batu City, East Java, with a focus on the Family Use Value (FUV) approach to identify the most utilized plant families in traditional medicine. Data were collected through structured interviews with 70 respondents selected using purposive and snowball sampling techniques. The study analyzed the FUV and Fidelity Level (FL) of various plant species to determine their significance in traditional healing practices. Results indicate that the Zingiberaceae and Euphorbiaceae families have the highest FUV (0.45), highlighting their widespread use in treating various ailments. Conversely, the Oxalidaceae family exhibits the lowest FUV (0.01). The FL analysis shows that Allium cepa (shallot) has the highest fidelity level (75%), demonstrating its prominence in treating multiple conditions. This research provides critical insights into the preservation of ethnomedicinal knowledge and the sustainable use of medicinal plant resources. The findings serve as a valuable reference for conservation efforts and the development of community-based herbal medicine initiatives.
Co-Authors Dian Saptarini Fitriani, Aprilia Dyah Kristanti Indah Purwani Rosyid Ridho, Muhammad Ainur Setyo Nugroho Trisnawati, Indah Tutik Nurhidayati