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DAYA ANTIFUNGI REBUSAN KULIT BATANG JAMBU METE (Anacardium occidentale L.) TERHADAP C. albicans PADA RESIN AKRILIK Ria Lidyawita; Sudarsono Sudarsono; Harsini Harsini
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Lack of dental care will be resulted in the increased colonies of C. albicans if the dental care was not in ordinary condition that can cause denture stomatitis. C. albicans is common in denture wearer and is found as many as 86% of patients with denture stomatitis. Cashew bark contains tannins, gallic acid and anacardic acid, phenolic compounds which are responsible for the antifungal activity against C. albicans. This research consisted of determination of total phenolic compound by Folin Ciocalteu method, and the antifungal test against C. albicans in acrylic resin plate. Acrylic resin plate was immersed in a suspension of C. albicans for 24 hours, rinsed with Phospate Buffered Saline (PBS), soaked with agitation for 3 minutes in a solution of cashew stem bark 12.5%w/v, 25%w/v, and 50% b/v with 20 mg/ml stock, vibrated in physiological saline, incubated on Sabouraud order for 48 hours. At the end of the experiment, the inhibition of C. albicans growth was observed and calculated. Anava and Kruskal-Wallis test were used to determine the significance difference between groups. Water extract of stem bark in the concentration of 12,5%w/v, 25%w/v, and 50% w/v had phenolic total value of 35,18% w/w EAG, 31,64%w/w EAG, 29,47%w/w EAG. It could inhibit C. albicans by 69.69%, 82.66%, 94.14% respectively.

PENGARUH KADAR FENOLIK PADA DAUN TEH Camelia xinensis L. TERHADAP PREFERENSI EMPOASCA SP. (Homoptera : Cicadellidae) Saiful Saiful; Sudarsono Sudarsono
Majalah Obat Tradisional Vol 18, No 2 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Phenolic compounds are secondary metabolites found in all parts of the plants aree.g.:fruits, barks, roots, and leaves of plants serve as chemical defenses as repellent against insects and herbivorous detterent. On tea leaves, the results of research gallocatechin (a derivate of catechin) was functioning known as mechanism of resistance to Empoasca sp. (Homoptera: Cicadelliadae). The aim of this research was to know the total phenolic content of the young tea leaf (Camelia sinensis L.) and it was to found out the effect of the phenolic compounds of preference Empoasca sp. The results obtained that the clone of “Pasir Sarongge” (PS) had total phenol content (polyphenol average ± SD) 25,69 ± 0,1 higher than Kiara clone 18,47 ± 0,13. The higher the polyphenol in the young tea leaf bud could be act as Empoasca sp. Detterent.

AKTIVITAS ANTIINFLAMASI REBUSAN KULIT BATANG JAMBU METE (Anacardium occidentale L.) PADA UDEMA KAKI TIKUS TERINDUKSI KARAGENIN Lini Veriony; Sudarsono Sudarsono; Agung Endro Nugroho
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Hot water extract of cashew nut’s stem bark (Anacardium occcidentale L.) traditionally has been used as mouth washer to heal mouth and gum inflammation. Combination of aqueous extract of cashew nut stem bark and grape juice have anti-inflammatory activity. Cashew nut stem bark contains gallic acid and anacardic acid that had anti-inflammatory effect. The aim of this research is to observe anti-inflammatory activity of the hot water extract of Cashew nut’s stem bark compared to Indomethacin Non-Steroid Anti-inflammatory Drug. This research’s process consists of collection of the stem barks, extraction of the stem bark by hot water, qualitative analysis of gallic acid and anacardic acid, measurement total phenolic value, free radical scavenging and antiinflamatory activity assay. The hot water extract of stem bark was used to observe anti-inflammatory activity by 1.25; 2.5; 5g/kgBB and as positive control was indomethacin 10 mg/kgBB. The result of edema volume determination were counted as area under curve value and anti-inflammatory percentage and then the data were analyzed to evaluate the data difference of every group. The anti-inflammatory activity of 1.25; 2.5 and 5g/kgBB were not significantly different compared to 10 mg/kg BB indomethacin. The total phenolic concentration was gradually decreasing with the increasing concentration of the hot water extract of stem bark. The free radical scavenging DPPH activity at 1mg/mL hot water extract of stem bark was poorly than gallic acid 1mg/mL.

PENGARUH PEMBERIAN EKSTRAK ETANOLIK RIMPANG JAHE MERAH TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes YS Bintari; Sudarsono Sudarsono; Agustinus Yuswanto
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Ginger is one of some medicinal plants that has been used to make someone still in the comfortable condition e.g. headache, diarrhea, flatulent, cold, cough, and masuk angin. The purpose of this study was to determine effects of pungent principle extract of the “jahe merah” rhizome in the relation of increasing macrophage phagocytic activation. This method was done by using latex beads.( Leijh et al., (1986) The effects of 10 mg/kgBW, 25 mg/kgBW, and 100 mg/kgBW of pungent principle extract of “jahe merah” rhizome ethanolic extract, that extracted by 70%v/v Ethanol was decided according to the number of latex which was phagocyted by macrophages. The conditioning time was done in 20 days. At 15th day, mice were infected by Listeria monocytogenes. The data was analyzed by Kolmogorov-Smirnov, and one way ANOVA with by 95% confidence level. As the specific parameter of the pungent principle extract were: pungent principle spot 1 : 2 = 6.8:1; 3.27%w/w GAE (Gallic Acid Equivalent) as total phenolic concentration, and IC50 14.57 mg/mL. The result was shown that 100 mg/kgBW of 70% ethanolic extract of “jahe merah” rhizome, was capable to increase macrophage activity. The effect of increasing macrophage phagocytosis of 100 mg/kgBW of 70% ethanolic extract of jahe merah was the same with Echinacea extract and Levamisol.

POTENSI PENANGKAPAN RADIKAL 2,2-difenil-1-pikril hidrazil (DPPH) OLEH BUAH PISANG SUSU (Musa paradisiaca L. “Susu”) DAN PISANG AMBON (Musa paradisiaca L. “Ambon”) Anjar Hermadi Saputro; Sudarsono Sudarsono
Majalah Obat Tradisional Vol 19, No 1 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Nutrients including vitamin A and C on milk banana and ambon banana predicted potential as an antioxidant. This study aims to determine the radical potential of pisang susu and pisang ambon with synthetic vitamin C as a comparison so we get how much antioxidant power of ambon banana and milk banana produced in the fruit if desired pengonsumsian antioxidant power of vitamin C produced by pure at certain levels. This study consisted of measuring the antioxidant activity by the method of 2,2-Diphenyl-1-Pikrilhidrazil (DPPH) so that the data obtained antioxidant activity and Inhibitory Concentration50 (IC50) of milk banana, ambon banana and vitamin C synthetic. From the research results can be concluded that as much as 2418.84g of milk banana as an antioxidant potential equivalent to the antioxidant potential of 1g of vitamin C when measured with 0.4 mM DPPH reagent, while the total of 9229.79g of ambon banana as an antioxidant potential equivalent to the potential antioxidants vitamin C 1 gram when measured with 0.4mM DPPH reagent.

PENGARUH PEMBERIAN EKSTRAK ZAT PEDAS RIMPANG JAHE EMPRIT YANG DISARI DENGAN ETANOL 70% TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes Dyah Mellawati; Sudarsono Sudarsono; Agustinus Yuswanto
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Immunomodulator has been used as one of the attention to develop herbal medicine. Jahe is one of herbal medicine which is usually used to heal various diseases. Jahe Emprit is one of local name of Jahe in Indonesia. The aim of this research was to investigate the effect of pungent principle containing extract of Jahe emprit rhizome on the macrophage activity compared by levamisol and Echinacea extract. Extraction was done by 70%v/v ethanol. The concentrations of extract were 5 mg/kg B.W; 25 mg/kg B.W; and 100 mg/kg B.W. CMC Na 1,5%w/v was used as solvent control. Levamisol 2,5 mg/kg B.W and Echinacea extract 10 mg/kg B.W were used as positive control. The macrophage ability were measured according to Leijh et al., (1986) that was basically on the amount of latex which was captured by macrophage. The extracts were orally administrated to the Swiss male mice at the dose of 0,2 ml/20 g B.W. The Macrophage activity was analyzed by one way ANOVA at 95% of confidence level. The extract which had specification of 35% relative concentration of pungent principles; 3,554% ± 0,145 % W/W total phenolic substances; 13,70 mg/ml antioxydative value (IC50) at the concentrations of 5 mg/kg B.W and 25 mg/kg B.W could increase phagocytosis ability. The ability of the extract at the concentration 25 mg/kg.B.W had no different with either the Levamizol 2,5 mg/kg. B.W., and Echinacea extract 2,5 mg/kg. B.W.

PENANGKAPAN RADIKAL 2,2-difenil-1-pikril hidrazil (DPPH) BUAH PEPAYA (Carica papaya L. (jingga)) TUA DAN MUDA Emir Ramadhan; Sudarsono Sudarsono
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Some compounds were potentially as a radical catcher, it was also found in the papaya fruit (Carica papaya L. (orentang)). This study aimed to determine 2,2-diphenyl-1-pycrylhydrazyl (DPPH) radical capture of ripe and raw papaya fruits with vitamin C synthetic as a benchmark. Mashed papaya pulp was dried by the freeze-drying method. The dryed mass was dissolved in ethanol and reacted with DPPH. Vitamin C was also treated by the same method, then made a standard and curve of percent DPPH radical arrest by vitamin C. Percent DPPH radical capture by the ripe and raw papaya fruit powder extracts was plotted on a standard curve. Ripe papaya fruit had the ability to capture the highest DPPH radical (1.340,931 mg of fresh ripe papaya proportional to 1 mg of vitamin C). The raw papaya had the capability of 3.557,814 mg of fresh raw papaya proportional to 1 mg of vitamin C.

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