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All Journal Indonesian Journal of Cancer Chemoprevention Jurnal Ilmu Ternak Veteriner
Dian R Laksmitawati
Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Veterinary

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Secretion of Indoleamine 2,3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell Laksmitawati, Dian R; Sardjono, Caroline Tan; Pawitan, Jeanne A.; Sadikin, Mohammad; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Lipoaspirate, a wasted by product from liposuction procedure recently has been shown to contain abundant adipose-derived-mesenchymal stem cells (MSCs). Mesenchymal stem cells (MSCs) have been studied in many research areas to regenerate many cell lineages. In addition, MSCs have immunomodulatory effect. This capability has been utilized in several clinical studies in hematopoetic stem cell and organ transplantation as a strategy to reduce the risk of Graft versus Host Disease (GvHD). It has been reported that the ‘stimulated’ MSC is able to secrete substances to suppress tissue rejection. One of the substances was known to be indoleamine 2,3-dioxygenase (IDO).  A previous study has  characterized the secretion of IDO by bone marrow-derived MSCs stimulated by an inflammatory mediator interferon gamma (IFN-γ). IDO has been detected using Western blot analysis and by High Performance Liquid Chromatography (HPLC) assay. The aim of this study was to detect the presence of IDO in AD-MSCs culture with and without INFγ stimulation. Our study showed that AD-MSC stimulated with IFN-γ significantly secreted high level of IDO as detected by Enzyme-Linked Immuno Sorbent Assay (ELISA). Despite its property as a proinflammatory mediator, IFN-γ has shown to be able to induce IDO secretion in MSC culture which suggests the immuno modulatory effect of MSC. This study clearly demonstrates the potential application of adipose-derived MSC in the immunomodulatory strategy for allogenic transplantation.  
Effect of methanol extracts of nony fruit on mice infected by RH strain of Toxoplasma gondii Subekti, Didik T; Sari, Eka S.P; Widiastuti, Dwi R; Haerlani, Rica; Fitri Diani, Eka; Iskandar, Tolibin; Laksmitawati, Dian R
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.196 KB) | DOI: 10.14334/jitv.v10i4.457

Abstract

Intraperitoneal infection of Type I Toxoplasma gondii on mice causes high mortality at a short time due to parasitic burden, immunosuppression, cell and tissue damage. The mice survival is increased after treated with drugs that reduce or destroy tachyzoite and modulate or recovered the immune system. Nony fruit (Morinda citrifolia) is popular as immunomudulator and has antoxoplasma properties. The purpose of this experiment is to evaluate the effect of ethanol extract of nony fruit and Fansidar® (pyrimethamine-sulfadiazine) to reduce tachyzoite and improve survival as well as immunomudulator on mice following toxoplasma infection. Mice was divided into six groups (10 mice respectively) consist of infected-non treated groups, infected + Fansidar®, infected + ethanol extract of nony on several doses (100, 50, 25%) and non infected-non treated groups. All mice on each groups were infected intraperitoneally by 5 x 106 and 2,5 x 103 RH strain of Toxoplasma gondii tachyzoite/mice respectively. The results have shown that Fansidar® was successfully to reduced tachyzoite and improved mice survival but the ethanol extract of nony fruit was failed.     Key Words: Survivality, Immunomodulator, Toxoplasma gondii, Nony extracts
Co-Authors Caroline Tan Sardjono Didik T Subekti Dwi R Widiastuti Eka Fitri Diani Eka S.P Sari Ferry Sandra Jeanne A. Pawitan Mohammad Sadikin Rica Haerlani Tolibin Iskandar