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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI Jurnal Kedokteran Hewan

Didik T Subekti

Balai Besar Penelitian Veteriner

Author-ID : 387116

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Articles

Immunopathogenecity of Different Types of Toxoplasma Gondii Subekti, Didik T; Arrasyid, Nurfida K
Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Toxoplasmosis is a zoonotic disease caused by Toxoplasma gondii . The disease was widely found in high prevalence around the world . Seroprevalence of human toxoplasmosis in Indonesia was 43 - 88% while toxoplasmosis in animals was reported 6 - 70%. In the past, clinically manifestation of toxoplasmosis only occurred in individu which has immunodeficient or immunosupression . Recently, more evident showed that individu which has immunocompetent was also able to develop clinical signs when infected by pathogenic T gondii (type I of T gondii) . In fact, the pathogenicity of T. gondii depends on the type or closet which originated from their clonal population . Each type has different implication on clinical immunopatho genesis . In this paper, the differences of biological character, immunopathogenicity and their clinical implication of T gondii clonal population structure are reviewed. Key words : Toxoplasma gondii, clonal population, immunopathogenecity

Study of Antigenicity and Immunogenicity Gra1 Protein from Toxoplasma gondii Subekti, Didik T
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

Toxoplasmosis is known as zoonotic disease caused by Toxoplasma gondii infection. This microorganism has ability to evade immune system by forming parasitophorus vacuole (PV) formed through the phagosome vacuole modification by secreting dense granule protein (GRA). Among GRAs protein, GRA1 was selected as candidate for vaccine development. However, it remains controvercial whether the protein has adequate antigenicity and strong immunogenicity which are suitable for vaccine candidate. Some researcher reported that DNA vaccine of GRA1 was able to induce cellular mediated immunity and proinflamatoric humoral immunity. In fact, another study demonstrated that GRA1 protein was only antigenic based on their molecular weight and bioinformatic analysis.The other studies also showed that GRA1 was considered as weak immunogen based on bioinformatic studies. The ability of GRA1 protein to stimulate immune responses, both humoral and cellular mediated immunity were seemly caused by adjuvant. Â Key words: Toxoplasma gondii, GRA1, cellular mediated immunity, humoral immunity

Development, Structure, Mechanism and Efficacy of Trypanocidal for Surra Subekti, Didik T
Indonesian Bulletin of Animal and Veterinary Sciences Vol 24, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Surra is a contagious disease due to Trypanosoma evansi infection and causes economic loss in animal husbandry, especially in African countries, South America, the Middle East and Asia. In Indonesia, in 2010 to 2011 Trypanosoma outbreak resulted in death of 1159 horses, 600 buffaloes and a cattle. Control of Surra is generally done by using trypanosidal for eradication of parasites in animals. Trypanosidal for Surra is still relying five drugs namely suramin, isometamidium, quinapyramine, diminazene and melarsomine. The drugs have been used since 1920 until now. Suramin, quinapyramine and isometamidium can be used for curative or prophylactic purposes due to the long elimination half-life in the body, while diminazene and melarsomine are applied just for curative purposes. The efficacy of trypanosidal is largely determined by the sensitivity of T. evansi strain which is existed in their area and should not be generalized. Key words: Trypanocidal, Surra, Trypanosoma evansi

Effect of methanol extracts of nony fruit on mice infected by RH strain of Toxoplasma gondii Subekti, Didik T; Sari, Eka S.P; Widiastuti, Dwi R; Haerlani, Rica; Fitri Diani, Eka; Iskandar, Tolibin; Laksmitawati, Dian R
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Intraperitoneal infection of Type I Toxoplasma gondii on mice causes high mortality at a short time due to parasitic burden, immunosuppression, cell and tissue damage. The mice survival is increased after treated with drugs that reduce or destroy tachyzoite and modulate or recovered the immune system. Nony fruit (Morinda citrifolia) is popular as immunomudulator and has antoxoplasma properties. The purpose of this experiment is to evaluate the effect of ethanol extract of nony fruit and FansidarÂ® (pyrimethamine-sulfadiazine) to reduce tachyzoite and improve survival as well as immunomudulator on mice following toxoplasma infection. Mice was divided into six groups (10 mice respectively) consist of infected-non treated groups, infected + FansidarÂ®, infected + ethanol extract of nony on several doses (100, 50, 25%) and non infected-non treated groups. All mice on each groups were infected intraperitoneally by 5 x 106 and 2,5 x 103 RH strain of Toxoplasma gondii tachyzoite/mice respectively. The results have shown that FansidarÂ® was successfully to reduced tachyzoite and improved mice survival but the ethanol extract of nony fruit was failed. Â Â Key Words: Survivality, Immunomodulator, Toxoplasma gondii, Nony extracts

Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite Subekti, Didik T; Artama, W.T; Sulistyaningsih, E; Poerwanto, S.H; Sari, Y; Bagaskoro, F
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain. Key words: GRA1, Toxoplasma gondii, Clonning, Expression

ANALISIS IMUNOGENISITAS PROTEIN GRA1 DARI HASIL KLONING GEN GRA1 TAKIZOIT Toxoplasma gondii [Immunogenicity Analysis of GRA1 Protein Derived from Clone Bearing GRA1 Genes Collected from Toxoplasma gondii Tachyzoite] Subekti, Didik T; Artama, WT; Poerwanto, SH; Sulistyaningsih, E; Sari, Yulia
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was aimed to analyze the immunogenicity of GRA1 protein derived from clone bearing GRA1 genes from local isolate of Toxoplasma gondii. Analysis of GRA1 protein translated from cDNA of GRA1 is very essential in prior to expressed the gene. Analysis of GRA1 protein derived from clone bearing GRA1 genes was performed using several bioinformatics software which are available as standalone or online software such as CLC Bio Workbench series, BioEdit, BESTORF, GENSCAN, FGENES, BepiPred 1.0, CTL Epitope Finder and SignalP. Translation coding sequences of GRA1 gene into GRA1 peptide sequences revealed 190 amino acids with molecular mass of GRA1 approximately 20.159 kD and isoelectric point at 4.43. GRA1 protein also identified several antigenic domains with six domains were known as epitopes for CD8+/cytotoxic lymphocyte and seven domain as epitopes for B lymphocyte. However, GRA1 protein was considered as good antigen but less immunogenic.

MORTALITAS DAN PROFIL HEMATOLOGI MENCIT YANG DIINFEKSI Trypanosoma evansi ISOLAT BANGKALAN, PEMALANG DAN PIDIE [Mortality and Haematology Profiles of Mice Infected with Trypanosoma evansi from Bangkalan, Pemalang and Pidie Isolates] Subekti, Didik T; Febria, Mutiara; Sari, Febiola Rama; Hartiyati, Indri N
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.531

Trypanosoma evansi known as trypanosomiasis causes or called surra in animals. Trypanosomiasis-associated death were generally due to severe anemia. Another report mentioned that a rapid death of mice can be attributed to the several reasons. One of those reasons is highly parasitaemia that leads to death of the host. However, there is another distinct report that high parasitemia in mice does not necessarily lead to anemia and clinical symptoms. This research was focused to study the relationship between trypanosomiasis-associated death with haematology profile, particularly the decrease of PCV (packed cell volume) value, anemia and virulence of T. evansi collected from several region of Indonesia. The experiments were performed by dividing mice into four groups. Three groups were infected by T.evansi according to the original isolates while another group were uninfected mice. Every two-day interval, all mice were observed their mortality and parasitaemia and also examined the PCV value and erythrocyte counts in peripheral blood. The experimental results shown that the degree of parasitemia in mice were not always related to mice mortality and anemia. Decreases of PCV value were related to the existence of parasitaemia but not with anemia. This study suggests that there is no relationship between the decreases of PCV value and anemia to the trypanosomiasis-associated death in mice.

Effect of Toxoplasma Infection Dosage on IgG, IgM, Fetus Weight and Body Length, and Necrosis of Placenta and Fetal Heart Nurinasari, Hafi; Sajidan; Purwanto, Bambang; Indarto, Dono; Subekti, Didik T
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.596-602

One of the most prevalent zoonotic illnesses in the world, toxoplasmosis, affects both humans and animals and is caused by the parasite Toxoplasma gondii. The infection will trigger the immune system to increase antibody production. This study aims to determine the dose of toxoplasma that causes necrosis in rats placental and fetal hearts. This study was laboratory experimental research with a Randomized Control Trial (RCT). The study design used a post-test only with a control group design. The Ig G variable obtained from the control group (CG) and treatment group 3 (TG3) is the most significant because the mean value difference was the largest (176.56). The Ig M variable obtained from the control group with treatment group 3 (TG3) is the most significant because the mean value difference is the largest (33.47). The fetus weight variable obtained from the control group with treatment group 3 (TG3) is the most significant because the mean value difference is the largest (2.6). The body length variable obtained from the control group between treatment group 3 (TG3) is the most significant because the mean value difference is the largest (1.26). There was a significant difference in placental tissue necrosis (p = 0.034) and heart (p = 0.025) between the control group (CG) and treatment group 3 (TG3). Therefore, there was a significant difference in Ig G, Ig M, fetus weight, body length, placental tissue necrosis, and fetal heart at the 10³ dose compared to the 102, 101, and normal doses.

COMPARISON OF DIFFERENT COMMERCIAL SEROLOGY KITS FOR THE DETERMINATION OF SEROPOSITIVE TOXOPLASMOSIS IN CATTLE IN INDONESIA Subekti, Didik T; Valinata, Sisca; Fong, Sulinawati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.21716

This research aims to explain the evaluation of the differences in four commercial kits for the detection of serological toxoplasmosis used in Indonesia. The results of the study found that the toxoplasmosis seropositivity determined by the four commercial kits showed a significant difference (P0.05). Seropositive toxoplasmosis obtained using Pastorex, Toxotest, IDScreen, and Toxo Ab were 35.12%, 60.12%, 26.19%, and 10.12% respectively. IDScreen had a good agreement with Toxo Ab (Gwet's AC1= 0.623) and a moderate agreement with Pastorex (Gwet's AC1= 0.494-0.511). Toxotest had a low agreement with three commercial kits (Gwet's AC1= 0.2) but had a moderate agreement with western blotting (WB) and modified agglutination test (MAT) (Gwet's AC1= 0.458-0.557).

COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL Yuniarto, Ichwan; Subekti, Didik T; Cahyaningsih, Umi; Satrija, Fadjar
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.11486

This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed thatthe molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, andLampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolatedfrom the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite beingisolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in thesame biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism ofeach Trypanosoma evansi isolate from Indonesia.

Co-Authors Bambang Purwanto Dian R Laksmitawati Dono Indarto Dwi R Widiastuti E Sulistyaningsih Eka Fitri Diani Eka S.P Sari F Bagaskoro Febiola Rama Sari, Febiola Rama Febria, Mutiara Fong, Sulinawati Hartiyati, Indri N Nurfida K Arrasyid Nurinasari, Hafi Poerwanto, SH Rica Haerlani S.H Poerwanto Sajidan Tamaulina Br Sembiring Tolibin Iskandar Umi Cahyaningsih Valinata, Sisca W.T Artama WT Artama, WT Y Sari Yuniarto, Ichwan

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