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All Journal Journal of Aceh Physics Society PROSIDING SEMINAR KIMIA Jurnal Kimia Mulawarman Indonesian Journal of Chemistry Jurnal Natural

Binawati Ginting

Jurusan KimiaFakultas Matematika dan Ilmu Pengetahuan AlamUniversitas Mulawarman

Author-ID : 3048526

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Neuroscience Physics

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ISOLATION OF ANTIOXIDANT COMPOUNDS OF METHANOL EXTRACT OF NUTMEG LEAVES (Myristica fragrans Houtt) Nurmilasari Nurmilasari; Binawati Ginting; Hira Helwati
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Abstract.The antioxidant activity from methanol extract of nutmeg leaves (Myristica fragrans Houtt) was performed using 2,2-diphenyl-1-picrylhydrazil (DPPH). Antioxidant assay using DPPH of the methanol extract at a concentration of 25 ppm, 50 ppm and 100 ppm, showed high antioxidant activity with IC50 = 36.31 ppm and Vitamin C showed antioxidant activity with IC50 = 3.657 ppm. The methanol extract of nutmeg leaves were partitioned with chloroform to obtain chloroform extract of the leaves with IC50 = 28.30 ppm. isolation of the active compounds from the chloroform extract of the nutmeg leaf using column chromatography yielded 6 mix fractions MFMD 1, MFMD 2, MFMD 3, MFMD 4, MFMD 5 and MFMD 6. The test results of antioxidant activity in very strong category is fractions MFMD 4, MFMD 3, MFMD 2, MFMD 6 and MFMD 5 with IC50 of each are 26.590 ppm, 27.239 ppm, 29.639 ppm, 39.766 ppm and 55.436 ppm, while antioxidant activity in a weak catagory is fraction MFMD 1 with IC50 is 126.270 ppm. This shows that the fraction of the leaf has a better inhibitory activity, because some of the active compound contained in the fraction can synergize in inhibiting free radicals, so that the IC50 value of the fraction is stronger than the extract. Keywords: Antioxidant, chromathography, Nutmeg (Myristica fragransHoutt), methanol extract, leaves

ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK Ilham Maulana; Binawati Ginting; Nurdin Nurdin; Saiful Fakri
Jurnal Natural Volume 19 Number 3, October 2019
Publisher : Universitas Syiah Kuala

Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.

ANTIOXIDANT ACTIVITY OF N-HEXANE EXTRACT OF NUTMEG PLANTS FROM SOUTH ACEH PROVINCE Binawati Ginting; Mustanir Mustanir; Hira Helwati; Lydia Septa Desiyana; Eralisa Eralisa; Rohmat Mujahid
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Abstract. It has tested the antioxidant activity of n-hexane extract respective roots, bark, fruit and seed crops of nutmeg against DPPH. Each extracted by maceration method with n-hexane. The extraction of each plant nutmeg with n-hexane extract obtained yield of n-hexane respectively 8,35%; 81,5%; 11,89% and 55,63%. The result of the antioxidant activity of n-hexane extract of each plant nutmeg against DPPH with a concentration of 25 ppm, 50 ppm and 100 ppm is obtained IC50, respectively, are 0,216 ppm, 63,755 ppm, 43,998 ppm and 11,599 ppm and positive control vitamin C ( IC50 = 3,657 ppm). Vitamin C is a standard compound is more often used than butyl for very high antioxidant activity. The antioxidant activity increases from high to low at the roots, seeds, fruits and bark of plants nutmeg in a row. Based on data GC-MS chemical components of each successive extracts of roots, bark, fruit and seeds are 41, 41, 44, 43 components of secondary metabolites. The main components of each part of the plant based on data from GC-MS are a 5-Octadecanoic acid, linalool, eugenol, myristicin, methoxyeugenol. The compounds are thought to play an active role in inhibiting free radicals from DPPH. n-Hexane extract nutmeg plant potential as an antioxidant. Keywords:Nutmeg (Myristica fragrans Houtt), n-hexane extract, Antioxidant, DPPH

Co-Authors Dedi Satria Eralisa Eralisa Fauzi Fauzi Hira Helwati Hira Helwati Ilham Maulana Juliawati Juliawati KHAIRI SUHUD Lelifajri Lelifajri Lydia Septa Desiyana Marlina Marlina Mustanir Mustanir Nurdin Nurdin Nurdin Nurdin Nurmilasari Nurmilasari Rahmi Rahmi Rohmat Mujahid Saiful Fakri Saiful Saiful Salfauqi Nurman, Salfauqi Siti Hadijah Surbakti, Muhammad Syukri Yuliani Aisyah Yuliani Yuliani

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