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All Journal Agrovigor: Jurnal Agroekoteknologi Jurnal Pertanian Agros
Heru Sudrajat
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Agriculture, Biological Sciences & Forestry

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UPAYA PENGAKARAN Echinacea purpurea L DENGAN AUKSIN SECARA KULTUR JARINGAN Heru Sudrajat
Agrovigor Vol 6, No 1 (2013): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.172 KB) | DOI: 10.21107/agrovigor.v6i1.1477

Abstract

Recently, to meet the needs of botanicals medicinal plants the cultivation directed, require good quality and uniform seeds on form and time.    Tissue culture research to from root of Echinacea purpurea L by the provision of IBA and NAA will be conducted.This recearched aimed to develop an and cultivate qualified Echinacea purpurea L as the raw material for drug foemulation. Tissue culture techniques has  more advantage because it is not affected by the climate, relatively fast production, free contamination of microbial and do not require large tracts of land. The research was carried out considering the need to be developed and cultivated plant Echinacea purpurea L qualified as raw material for the drug tissue culture techniques has advantages because it is not affected by the climate with relatively fast production time, free of microbial contamination and do not require large tracts of land. Shoots multipication done on MS medium using growth regulators GA3with concentration of 3 mg / l. Research foor rof induction on Echinacea purpurea L shoot made by adding growth regulators of IBA and NAA with concentrations with 0, 2, 4 and 6 mg / l respectively. The 2 months incubation periods produced tehe best root induction on the additon of IBA 4 mg / l. It producted 16 roots with a length of 4 cm and relatively lagre in size.Keywords: Echinacea purpurea L., tissue culture, IBA, NAA, plant growth regulators
THE EFFECTS OF BAP AND 2,4 D ON INITIATION AND INDUCTION OF PULESARI (Alyxia reinwardtii Blume) CALLUS Nur Rahmawati Wijaya; Didik Suharto; Heru Sudrajat
Agros Journal of Agriculture Science Vol 19, No 1 (2017): Edisi Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.69 KB)

Abstract

Utilization of Pulesari as medicinal plant is less optimal due to scarcity and less known techniques of cultivation. To increase production of plant propagation it can use plant tissue culture especially callus formation. Research purpose: study effect of BAP and 2,4 D and knowing most appropriate hormone concentration for induction of Pulesari callus. Research using MS basal media with combination BAP 1-4 ppm and 2,4 D 1-4 ppm. Explant used pulesari leaf. Results: for three months showed treatment BAP + 2,4 D 4 ppm 1 ppm give fastest callus grow in 23 days after planting and highest amount of callus with surface area 3,4 cm2. Treatment of BAP 3 ppm + 2,4 D 1 ppm, as well as 4 ppm BAP + 2,4 D 4 ppm produce green callus, treatment of BAP 1 ppm + 2,4 D 1 ppm showed a yellowish white color, treatment BAP 1 ppm + 2,4 D 2 ppm, 2 ppm BAP + 2,4 D 4 ppm, and BAP 3 ppm + 2,4 D 4 ppm produce white callus. Callus with crumb texture produced by treatment of BAP 1 ppm + 2,4 D 3 ppm, BAP 1 ppm + 2,4 D 4 ppm, BAP 3 ppm + 2,4 D 2 ppm and BAP 3 ppm + 2,4 D 3 ppm while compact callus treatment resulted by BAP 2 ppm + 2,4 D 2 ppm, BAP 2 ppm + 2,4 D 3 ppm, BAP 4 ppm + 2,4 D 2 ppm and BAP 4 ppm + 2,4 D 3 ppm. Conclusion: BAP 4 ppm and 2,4 D 1 ppm accelerate callus grow. Best treatment is BAP 4 ppm+2,4D 1 ppm with hinghest amount of callus and fastest grow.
Co-Authors Didik Suharto Nur Rahmawati Wijaya