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All Journal Agrovigor: Jurnal Agroekoteknologi Jurnal Pertanian Agros
Didik Suharto
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Agriculture, Biological Sciences & Forestry

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PENGARUH NAA DAN BAP TERHADAP EKSPLAN PEGAGAN (Centella asiatica (L.) Urb.) Heru Sudrajad; Didik Suharto; Fauzi .
Agrovigor Vol 8, No 1 (2015): MARET
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v8i1.744

Abstract

Pegagan (Centella asiática (L.) Urb.) representing one of theplant which good of as drug. Plantof pegagan (Centella asiática (L.) Urb.) good of  to launch the urine, degrading blood pressure and quicken to heal the hurt. Ready of the seed for the crop of drug which require to be paid attention by quality from itself seed. One of the alternative to get the uniform seed and a flash in the pan is with the technique of tissue culture. Tissue culturelaboratory research was conducted Researchand Development Center for Medicinal Plantsand Traditional MedicineTawangmangu. Research Method use the Random Device of Complete Group at MS (Murashige Skoog) media with the treatment ofplant growth regulator the NAA concentration 0, 1, 3 and 5 mg / l and BAP concentration 0, 1, 2, 3 and 4 mg / l). Result of research show the combination of  giving of NAA 1 until 3 mg / l and BAP 1 until 5 mg / l of is condition of explan experience of the change become the callus. Treatment of combination NAA 3 mg / l and BAP 4 mg / l give the best result to callus forming with the quicker callus forming time that is 25 day. Keywords : Pegagan, Centella asiática (L.) Urb., tissue culture, NAA, BAP
UPAYA INISIASI KALUS TABAT BARITO (Ficus deltoidea Jack) DENGAN KULTUR JARINGAN Heru Sudrajad; Didik Suharto
Agrovigor Vol 7, No 1 (2014): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.413 KB) | DOI: 10.21107/agrovigor.v7i1.1425

Abstract

THE EFFECTS OF BAP AND 2,4 D ON INITIATION AND INDUCTION OF PULESARI (Alyxia reinwardtii Blume) CALLUS Nur Rahmawati Wijaya; Didik Suharto; Heru Sudrajat
Agros Journal of Agriculture Science Vol 19, No 1 (2017): Edisi Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.69 KB)

Abstract

Utilization of Pulesari as medicinal plant is less optimal due to scarcity and less known techniques of cultivation. To increase production of plant propagation it can use plant tissue culture especially callus formation. Research purpose: study effect of BAP and 2,4 D and knowing most appropriate hormone concentration for induction of Pulesari callus. Research using MS basal media with combination BAP 1-4 ppm and 2,4 D 1-4 ppm. Explant used pulesari leaf. Results: for three months showed treatment BAP + 2,4 D 4 ppm 1 ppm give fastest callus grow in 23 days after planting and highest amount of callus with surface area 3,4 cm2. Treatment of BAP 3 ppm + 2,4 D 1 ppm, as well as 4 ppm BAP + 2,4 D 4 ppm produce green callus, treatment of BAP 1 ppm + 2,4 D 1 ppm showed a yellowish white color, treatment BAP 1 ppm + 2,4 D 2 ppm, 2 ppm BAP + 2,4 D 4 ppm, and BAP 3 ppm + 2,4 D 4 ppm produce white callus. Callus with crumb texture produced by treatment of BAP 1 ppm + 2,4 D 3 ppm, BAP 1 ppm + 2,4 D 4 ppm, BAP 3 ppm + 2,4 D 2 ppm and BAP 3 ppm + 2,4 D 3 ppm while compact callus treatment resulted by BAP 2 ppm + 2,4 D 2 ppm, BAP 2 ppm + 2,4 D 3 ppm, BAP 4 ppm + 2,4 D 2 ppm and BAP 4 ppm + 2,4 D 3 ppm. Conclusion: BAP 4 ppm and 2,4 D 1 ppm accelerate callus grow. Best treatment is BAP 4 ppm+2,4D 1 ppm with hinghest amount of callus and fastest grow.
Co-Authors Fauzi . Heru Sudrajad Heru Sudrajat Nur Rahmawati Wijaya