Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Jurnal Tumbuhan Obat Indonesia Science and Technology Indonesia Jurnal Pertanian Agros
Nur Rahmawati Wijaya
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
PENGARUH KINETIN DAN NAA TERHADAP INDUKSI KALUS PULE PANDAK (Rauvolfia serpentina (L.) Benth. ex Kurz) heru Sudrajad; Nur Rahmawati Wijaya
Jurnal Tumbuhan Obat Indonesia Vol 12 No 2 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.655 KB) | DOI: 10.22435/jtoi.v12i2.1691

Abstract

ABSTRACT Pule pandak is a rare medicinal plant with low regeneration since it only depend on natural propagation. This plant is difficult to be propagated while the demand of its material for herbal and pharmaceutical industries increased by time. The aim of this research was to find the best method of pulepandak callus induction. This research used pulepandak leaves as explant and Nitsch as base media. The experiment conducted by using combination of Kinetin and NAA as growth hormones. The hormones concentration as follows: Kinetin 1 mg/l, 2 mg/l, Kinetin 3 mg/l and NAA concentration were 0 mg/ml (control) and 1 mg/l. The results showed that the treatment of growth regulators combination of Kinetin 2 mg/l + NAA 0 mg/l, Kinetin 2 mg/l + NAA 1 mg/l, Kinetin 3 mg/l + NAA 0 mg/l and Kinetin 3 mg/l + NAA 1 mg/l were able to induce callus on R. serpentine explant while treatment of kinetin 3 mg/l and NAA 1 mg/l induced callus and roots.. ABSTRAK Pule pandak merupakan salah satu tanaman obat langka yang regenerasinya lambat karena hanya mengandalkan perbanyakan secara alami. Tumbuhan ini sulit dibudidayakan sementara kebutuhan sebagai bahan baku dalam industri herbal dan farmasi semakin meningkat. Penelitian ini bertujuan untuk mengetahui perlakuan terbaik untuk pembentukan kalus pulepandak. Dalam penelitian ini digunakan daun pulepandak sebagai eksplan dengan media dasar Nitsch dengan pemberian zat pengatur tumbuh kinetin dengan konsentrasi 1, 2 dan 3 mg/l serta NAA konsentrasi 0 mg/l dan 1 mg/l. Hasil penelitian menunjukkan bahwa perlakuan zat pengatur tumbuh kombinasi kinetin 2 mg/l dan 3 mg/l serta NAA 1 mg/l dan NAA 0 mg/l mampu menginduksi kalus pada eksplan R. serpentina, sementara kombinasi kinetin 3 mg/l dan NAA 1 mg/l mampu menginduksi pertumbuhan kalus dan akar.
THE EFFECTS OF BAP AND 2,4 D ON INITIATION AND INDUCTION OF PULESARI (Alyxia reinwardtii Blume) CALLUS Nur Rahmawati Wijaya; Didik Suharto; Heru Sudrajat
Agros Journal of Agriculture Science Vol 19, No 1 (2017): Edisi Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.69 KB)

Abstract

Utilization of Pulesari as medicinal plant is less optimal due to scarcity and less known techniques of cultivation. To increase production of plant propagation it can use plant tissue culture especially callus formation. Research purpose: study effect of BAP and 2,4 D and knowing most appropriate hormone concentration for induction of Pulesari callus. Research using MS basal media with combination BAP 1-4 ppm and 2,4 D 1-4 ppm. Explant used pulesari leaf. Results: for three months showed treatment BAP + 2,4 D 4 ppm 1 ppm give fastest callus grow in 23 days after planting and highest amount of callus with surface area 3,4 cm2. Treatment of BAP 3 ppm + 2,4 D 1 ppm, as well as 4 ppm BAP + 2,4 D 4 ppm produce green callus, treatment of BAP 1 ppm + 2,4 D 1 ppm showed a yellowish white color, treatment BAP 1 ppm + 2,4 D 2 ppm, 2 ppm BAP + 2,4 D 4 ppm, and BAP 3 ppm + 2,4 D 4 ppm produce white callus. Callus with crumb texture produced by treatment of BAP 1 ppm + 2,4 D 3 ppm, BAP 1 ppm + 2,4 D 4 ppm, BAP 3 ppm + 2,4 D 2 ppm and BAP 3 ppm + 2,4 D 3 ppm while compact callus treatment resulted by BAP 2 ppm + 2,4 D 2 ppm, BAP 2 ppm + 2,4 D 3 ppm, BAP 4 ppm + 2,4 D 2 ppm and BAP 4 ppm + 2,4 D 3 ppm. Conclusion: BAP 4 ppm and 2,4 D 1 ppm accelerate callus grow. Best treatment is BAP 4 ppm+2,4D 1 ppm with hinghest amount of callus and fastest grow.
Phytochemical Profile, Antioxidant Activity and Anticancer Activity of Gamma-Irradiated Black Rice Bran (Oryza sativa L.) Ethanolic Extract: In-Vitro and In-Silico Study Rukmana, Rizal Maarif; Silfarohana, Rantika; Putra, Aditya Dwi Permana; Devi Safrina; Dian Susanti; Nur Rahmawati Wijaya; M Bakti Samsu Adi; Muhammad Evy Prastiyanto; Yanuar Ashari Cahyaningrum; Rina Nurmaulawati; Weka Sidha Bhagawan
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.628-643

Abstract

Black rice is a food crop with black pigments, which helps reduce the risk of various diseases and improve health. One way to improve food quality and avoid food contaminants is by irradiating. This study evaluated the phytochemical profile, antioxidant activity, and anticancer activity of irradiated black rice bran extract (IBRBE) in vitro and in silico. Black rice was irradiated with a Gamma cell 220 type irradiator at a 7.5 kGy/hour dose rate. Extraction of irradiated black rice bran was carried out using the maceration method. Phenolic and flavonoid components were quantified using the Folin–Ciocalteau and AlCl3 methods, respectively. Phytochemical compounds were identified by liquid chromatography-high-resolution mass spectrometry (LC HRMS). The antioxidant activity of IBRBE was carried out against 2,2-diphenyl-1picrylhydazyl (DPPH). The cytotoxic activity of IBRBE against WiDr cells (colorectal cancer) and Vero (nonhuman cell lines) used the MT test method. Prediction of the inhibitory mechanism of compounds in the extract against target proteins EGFR and GPX7 was carried out in silico. Total phenolics and flavonoids were 2.57 ± 0.28 mg GAE/g and 19.12 ± 0.18 μg QE/ml, respectively. Twenty-four types of active compounds were obtained in IBRBE. The results of antioxidant activity obtained an IC50 value of 1198.45 ± 92.86 μg/ml. IC50 in WiDr cells and Vero cells were obtained at 36.08 ± 11.71 μg/ml and 570.58 ± 130.25 μg/ml, respectively. In silico results, the compound 4 Dodecylbenzene sulfonic acid has the highest binding affinity to the EGFR protein, with a value of -5.9. Meanwhile, the Monoolein compound has the highest binding affinity to the GPX7 protein, with a value of -5.4.
Co-Authors Devi Safrina Dian Susanti Didik Suharto heru Sudrajad Heru Sudrajat M Bakti Samsu Adi Muhammad Evy Prastiyanto Putra, Aditya Dwi Permana Rina Nurmaulawati Rizal Maarif Rukmana, Rizal Maarif Silfarohana, Rantika Weka Sidha Bhagawan Yanuar Ashari Cahyaningrum