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All Journal AGROEKOTEKNOLOGI
Bertryda Hastipa Manurung
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry

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The Combination 2,4-D and BAP to Induce Embryogenic Callus from Some Varieties of Soybean (Glycine max (L.) Merrill) on Hypoxic and In Vitro Condition Bertryda Hastipa Manurung; Revandy Iskandar Damanik; Eva Sartini Bayu
Jurnal Agroekoteknologi Vol 6, No 1, Januari (2018)
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.015 KB) | DOI: 10.32734/jaet.v6i1, Januari.17223

Abstract

The purpose of this research was to determinate the growth response of embryogenic callus from some varieties of soybean (Glycine max (L.) Merrill) on hypoxic and in vitro condition. The research was conducted at The Tissue Culture Laboratory of Agriculture Faculty in University of Sumatera Utara on April until December 2016. This research had two stages, induction of embryogenic callus and analysis metabolism of callus after hypoxic condition with t-test. This analysis used factorial Completely Randomized Design with two factors. The first factors were varieties of soybean and the second factors were combination of Plant Growth Regulator (PGR) (5 mg/l 2,4-D + 1 mg/l BAP, 10 mg/l 2,4-D + 1,5 mg/l BAP, and 15 mg/l 2,4-D + 2 mg/l BAP). The result showed the varieties, combination of PGR, and interaction of varieties and PGR give significant effect to weight callus. The result of T-test showed that in hypoxic condition, POD enzyme exercise on Gepak Kuning’s callus in 5 mg/l 2,4-D + 1 mg/l BAP was different before and after hypoxic condition. Keywords : 2,4-D, BAP, embryogenic callus, hypoxic, soybean
Co-Authors Eva Sartini Bayu Revandy Iskandar Muda Damanik