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Heat Resistance of Probiotic Candidate Enterococcus faecalis R22B in Different Matrices Shanti Dwita Lestari; Rinto Rinto; Indah S Wahyuni; Sherly Ridhowati; Wulandari Wulandari
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 1 (2021): May 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.497

Abstract

Fermented fish, including rusip and bekasam, contains lactic acid bacteria, some of which have probiotic properties. Considering the beneficial effect, the count of viable cells in probiotic carrier foods must be at least 106 CFU/g throughout the product shelf-life. The processing and storage condition significantly affect the viability of probiotics in carrier foods. This research was conducted to determine the heat resistance of probiotic candidate Enterococcus faecalis R22B isolated from rusip in three different media: bekasam, rusip, and physiological saline solution (NaCl 0.85%). E. faecalis R22B was heated in each medium at a temperature of 70 °C for 0, 2, 4, 8, and 12 min. The media characteristics showed differences within the parameters included pH values of 4.41-6.99; aw 0.67-0.92; moisture content 72.56-98.09%; ash content 0.69-12.32%; protein content 0-14.7%; fat content 0-0.84%; and carbohydrate content from 0-4.93%. The results showed that the difference in the heating medium had no significant effect (p0.05) on the heat resistance of the bacteria, as indicated by the D70 value. The required average heating time at 70 oC to decrease E. faecalis R22B cells by 1log10 (D70 value) in bekasam, rusip, and physiological saline media were 3.17, 3.29, and 3.55 min, respectively. Despite having a lower DT-value, the reduction of bacteria cells in three media heated at 70 oC showed no difference (p0.05); therefore, bekasam and rusip are suitable for probiotic carrier food.
Partial Characterization of Chitosanase from Bacillus cereus Strain BFE5400 Isolated from Snakehead Fish Intestine Shanti Dwita Lestari; Ace Baehaki; Guttifera Guttifera
Jurnal Perikanan Universitas Gadjah Mada Vol 22, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.43746

Abstract

Feeding pattern of wild snakehead fish (Channa striata) which includes shrimp and small crustaceans makes it is intestine a good source of chitinase and chitosanase enzymes. This study aimed to isolate chitosanase producing bacteria from snakehead fish intestine, to select isolate with highest chitosanolytic activity, to produce chitosanase using the selected isolate and partially characterize the resulting enzyme. I2 isolate exhibited the highest chitosanolytic index with the value of 1.5 and produced enzyme with an activity of 0.00331 U/mL after 72 hours incubation. The ultimate bacterial density was at the 24th hour with a turbidity value of 0.5315. The enzyme activity was optimum at pH 6, with a value of 0.00816 U/mL, while the optimum temperature was 70°C with the enzyme activity of 0.00785 U/mL. Genetic identification of I2 isolate using 16S rRNA showed that the isolate has a high similarity to Bacillus cereus strain BFE 5400.
Karakteristik Sensoris Kemplang Panggang Udang putih (Litopenaeus vannamei) dengan Penambahan Asap Cair Dwi Inda Sari; Herpandi Herpandi; Shanti Dwita Lestari; Rinto Rinto; Ambarwati Ambarwati
Jurnal FishtecH Vol 9, No 1 (2020)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v9i1.10315

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan asap cair terhadap sifat fisik, kimia dan sensori kemplang panggang udang putih (Litopenaeus vannamei). Metode penelitian ini menggunakan rancangan acak kelompok (RAK) dengan satu faktor perlakuan dan dilakukan dengan 3 kali ulangan. Perlakuan yang digunakan yaitu perbedaan konsentrasi asap cair (0%, 2,5%, 5% dan 7,5%). Berdasarkan hasil uji sensori penelitian tahap pertama didapatkan formulasi A3 sebagai formulasi terbaik dengan proporsi udang:tepung:air dengan nilai 1:2:1/2. Formulasi terbaik digunakan untuk tahapan pembuatan kemplang panggang udang putih. Hasil uji mutu hedonik menunjukkan bahwa tingkat mutu hedonik panelis mencapai 8, dengan tingkat kesukaan tertinggi yaitu di perlakuan A4 untuk parameter kenampakan, aroma, tekstur, sedangkan untuk rasa, nilai tertinggi di perlakuan A2. Perlakuan terbaik dari penelitian ini yaitu pada perlakuan A4 dengan konsentrasi asap cair 7,5%.
Pendampingan Analisis Gizi dan Sosialisasi Sertifikasi Halal Produk Bekasam Di Desa Meranjat Ogan Ilir, Sumatera Selatan Rinto, Rinto; Herpandi, Herpandi; Hanggita, Siti; Lestari, Susi; Ridhowati, Sherly; Lestari, Shanti Dwita; Pitayati, Puspa Ayu; Yunindyawati, Yunindyawati
Swadaya: Jurnal Pengabdian Masyarakat Vol. 2 No. 1 (2024): Swadaya: Jurnal Pengabdian Masyarakat
Publisher : Nuban Jaggadhita Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62265/swadaya.v2i1.49

Abstract

Purpose: The aim of this community service activity is to provide information on the importance of HALAL and the steps to obtain HALAL certification for UMKM. Apart from that, it also provides information on the importance of listing the nutritional composition on product labels as a communication medium between bekasam producers and consumers. Methodology: The method used is socialization of HALAL certification and assistance with nutritional analysis of bekasam. Results: The success of the activity can be measured through pretest and posttest parameters as well as the results of laboratory analysis, nutritional content of bekasam. All activities starting from preparation, socialization and nutritional analysis went well. Participants can understand halal certification material above 50%. Bekasam has nutritional content (chemical composition) in the form of protein (23.39%), fat (7.73%), minerals (11.07%), carbohydrates (1.37%), and water (56.44%).
SOSIALISASI DAN PENDAMPINGAN PELAKSANAAN SANITASI DAN HIGIENE PEMBUATAN BEKASAM DI DESA MERANJAT OGAN ILIR SUMATERA SELATAN Rinto Rinto; Indah Widiastuti; Susi Lestari; Rodiana Nopianti; Shanti Dwita Lestari; Gama Dian Nugroho
Jurnal Pengabdian Masyarakat Bumi Rafflesia Vol. 8 No. 1 (2025): APRIL: Jurnal Pengabdian Kepada Masyarakat Bumi Raflesia
Publisher : Universitas Muhammadiyah Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mutu dan kemanan pangan merupakan salah satu parameter standar yang harus dipenuhi produk pangan. Pada industri makanan ataupun usaha mikro kecil menengah (UMKM), salah satu usaha yang bisa dilakukan untuk menjaga mutu produk pangan adalah dengan menerapkan sanitasi dan higiene selama proses produksi/pengolahan. Oleh karena itu, perlu dilakukan kegiatan sosialisasi sanitasi dan higiene pada industri (UMKM) produk pangan yang bertujuan memberikan transfer knowledge tentang sanitasi dan higiene yang dilaksanakan di UMKM bekasam Desa Meranjat 2, Kecamatan Indralaya Selatan, Ogan Ilir. Metode yang digunakan yaitu sosialisasi secara langsung melalui penyuluhan dan pendampingan pelaksanaan sanitasi dan higiene. Kegiatan penyuluhan dilaksanakan kepada lebih dari 30 UMKM di Desa Meranjat 2. Penyuluhan dilakukan dengan memberikan materi secara langsung dan melalui penyebaran/pembagian leaflet. Kegiatan berjalan dengan baik yang dilihat dari pengetahuan terkait sanitasi dan higiene meningkat dari 23% menjadi 78%. Kegiatan pendampingan sebagai upaya evaluasi juga dilakukan untuk melihat secara langsung terhadap pelaksanaan kegiatan proses produksi bekasam dan produk perikanan lainnya di Desa Meranjat 2, Kecamatan Indralaya Utara, Kabupaten Ogan Ilir, Sumatera Selatan.