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Dwi Priyowidodo
Departemen Parasitologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences Veterinary

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Studi Respon Imun Humoral Mencit, Tikus, dan Ayam terhadap Infeksi Toxoplasma Gondii Gifti Rosalina Ratnaningrum; Dana Meida; Andhini Mutiara Putri; Hilda Syara Shita Devi; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6673.961 KB) | DOI: 10.22146/jsv.27556

Abstract

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. T. gondii is an obligate intracellular parasite that is transmitted by congenital and acquisition. T. gondii can infect all warm-blooded animals and are zoonotic. It is estimated that more than a third of the world’s population is infected by T. gondii. Chicken is one of the animals that can be infected with T. gondii and plays an important role in the spread of this disease to humans because it is one of the animals used as a source of animal feed. Mice and rats are also play an important role in the spread of toxoplasmosis in the definitive host, especially in cats. T. gondii infection is usually asymptomatic, but in certain circumstances, such as when the host’s immune system is weakened, this parasite can causeserious illness. This study were aimed to determine the humoral immune response against T. gondii infection in mice, rats and chickens by serological Latex Agglutination Test (LAT) using Pastorex-Toxo® kit. Two weeks oldbroiler chickens were 8 heads, Wistar strain rats aged 8 weeks as many as 18 heads and strain Balb C mice aged 8 weeks as many as 13 cows used in this study. Animals were grouped into two groups, namely the control andinfection groups. Mice, rats, and chickens were infected each with 103, 107 and 105 takizoit by intra-peritoneal injection. Blood samples were taken from the animals and infection control from day to-1, 2, 3, 4, 5, 6 for mice,the to-1, 2, 3, 4, 5, 6, 7, 8 for rats, and day - 2, 4, 14 for chicken by intracardia 3 ml, then put in a non-EDTA tubes and centrifuged at a speed of 10,000 rpm for 5 minutes to collect serum. The results showed a humoral immuneresponse against T. gondii in rats began to appear on day 5 post-infection, chickens began to emerge the 2nd day post infection, whereas mice do not indicate a humoral immune response against T. gondii post infection.
Akumulasi Fibrin dalam Anterior Chamber Pada Kucing Penderita Tripanosomiasis dan Feline Immunodeficiency Virus Kurnia Kurnia; Dyah Kunthi Wirapratiwi; Setyo Budhi; Guntari Titik Mulyani; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.54614

Abstract

Tripanomiasis merupakan penyakit yang disebabkan oleh Tripanosoma sp, protozoa hemoflagellata dari kelas Zoomatigophora dan famili Tripanosomatidae. Tripanosomiasis banyak dijumpai di daerah tropis dan menyerang berbagai hewan domestik seperti kuda, sapi, kerbau, onta, anjing, kucing dan tikus. Feline immunodeficiency virus (FIV) dikenal sebagai  feline AIDS adalah spesies virus dalam genus Lentivirus, menyebabkan penurunan sistem imun pada kucing dimana tubuh tidak dapat mengatasi serangan dari berbagai sumber penyakit lain sehingga muncul infeksi tambahan. Umumnya kucing tidak menimbulkan gejala klinis infeksi FIV meskipun telah berlangsung beberapa tahun. Seekor kucing domestik jantan, 3 tahun didiagnosis Tripanosomiasis dan positif FIV. Kedua mata terlihat berwarna keputihan, berawal dari mata kiri, berukuran kecil yang berkembang secara progresif selama 2 minggu, diikuti penurunan nafsu makan serta kondisi badan yang semakin kurus. Kucing berasal dari kucing jalanan, dan gemar memakan tikus maupun burung, serta belum dilakukan vaksinasi. Hasil pemeriksaan klinis menunjukkan mukosa anemik, dehidrasi, oedema di daerah submandibular hingga bahu, BCS 4/9, dan uveitis anterior. Hasil pemeriksaan hematologi dan kimia darah menunjukkan anemia normositik-hipokromik, trombositophenia, normal leukosit total dengan peningkatan relatif monosit, nilai SGPT dan SGOT yang sangat tinggi, peningkatan creatinin dan penurunan total kolesterol. Hasil pemeriksaan rapid test menunjukkan positif antibodi FIV (Feline Immunodeficiency Virus), negatif Feline Leukemia Virus, serta negatif  toksoplasma. Pemeriksaan apus darah menunjukkan mild anemia tanpa polikromasia, dan ditemukan flagellata Trypanosoma sp. Akumulasi fibrin di dalam anterior chamber yang bersifat progresif-bilateral disertai dengan aqueous flare dan normal retina merupakan gambaran anterior uveitis sebagai gejala klinis yang menciri dari Tripanosomiasis pada kucing dan infeksi FIV.
PREVALENSI DAN INSIDENSI PARASIT PADA IKAN MAS (CYPRINUS CARPIO) . Dwilantiani; Yudhi Ratna Nugraheni; Joko Prastowo; Dwi Priyowidodo; Ana Sahara; Wisnu Nurcahyo
Partner Vol 24, No 2 (2019): Edisi November 2019
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i2.285

Abstract

Infestasi parasit pada ikan dalam jumlah besar dapat menyebabkan kerugian ekonomi yang cukup signifikan. Parasit dari jenis Trematoda monogenean dan protozoa mendominasi infestasi tersebut.  Penelitian ini dilakukan untuk mendapatkan status kejadian infestasi parasit pada ikan mas biasa (Cyprinus carpio). Sebanyak 200 ikan mas (Cyprinus carpio) dikumpulkan dari kolam-kolam di Sleman dan Magelang, Jawa Tengah Indonesia. Metode pemeriksaan menggunakan metode wet mount. Parasit yang ditemukan sebagai berikut: Ichthyopthirius multifilis 41% Dactylogyrus  spp. 44,5% dan Gyrodactylus spp. 25%. Kejadian insidensi parasit sebagai berikut: Ichthyopthirius multifilis 1496/82, Dactylogyrus  spp. 119/37 dan Gyrodactylus spp. 2/2. Kondisi kolam yang buruk dan pergantian musim di Indonesia merupakan faktor yang sangat berpengaruh terhadap kejadian infestasi parasit pada ikan mas (Cyprinus carpio).
Parasit Gastrointestinal pada Sapi di Daerah Aliran Sungai Progo Yogyakarta Yudhi Ratna Nugraheni; Dwi Priyowidodo; Joko Prastowo; Eryl Sri Rohayati; Ana Sahara; Aan Awaludin
Jurnal Ilmu Peternakan Terapan Vol 1 No 2 (2018): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of study was investigate gastrointestinal disease on cow fed forage from Progo watershed. Sixty three feses samples were taken from cows on Progo watershed. The method of feses analysis used was sentrifuge and Parfitt Bank. The results showed that 63 samples of cows feces were examined, among 25 were infected by Fasciola sp., 7 Paramphistomum sp., 14 Strongyle, and 9 Coccidiosis. The conclusion was the prevalence of gastrointestinal disease in cows was dominant incidence of Fasciolosis by 40%, Strongyle 22%, 14% Coccidiosis, and Paramphistomum sp.11%.
Diagnosis dan Pengobatan Babesiosis pada Anjing Shih-Tzu di Klinik Lilipoet, Yogyakarta, Indonesia Nabilah Putroe Agung; Dwi Priyowidodo; Ida Tjahajati; Lily Gunawan
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.74202

Abstract

Dogs are one of the pet animals that have a loyal nature and are widely cared for in the community. It is not uncommon for dogs to often play in parks or outdoor environments that are surrounded by grass to play with their owners. The grass is a breeding ground for ticks, which can be vectors of disease carriers for dogs. Babesiosis is a disease caused by Babesia sp. and is transmitted by tick vectors. In this paper, we will discuss the clinical condition, diagnosis, and treatment of one dog with babesiosis at the Lilipoet clinic, Yogyakarta. A Shih-Tzu dog has clinical symptoms of fever with a temperature of 39.9oC, decreased appetite, and has a history of yellow vomiting, and found one tick. Blood was collected for microscopic examination of blood smears, blood hematologic, and molecular examination. Hematologic examination of the blood showed the value of thrombocytopenia with a value of 32 x 103/µL. Molecular examination with Polymerase Chain Reaction (PCR) showed positive results of Babesia sp. with visible DNA bands at 490bp. Treatment was carried out by giving one dose of antiprotozoal diminazen aceturate 3.5 mg/kg BW and doxycycline 10mg/kg BW once a day for 14 days.
Co-Authors . Dwilantiani Ana Sahara Ana Sahara Andhini Mutiara Putri Asmarani Kusumawati Awaludin, Aan Dana Meida Dyah Kunthi Wirapratiwi Eryl Sri Rohayati Gifti Rosalina Ratnaningrum Guntari Titik Mulyani Hilda Syara Shita Devi Ida Tjahajati Joko Prastowo Kurnia Kurnia Lily Gunawan Nabilah Putroe Agung Nugraheni, Yudhi Ratna Raden Wisnu Nurcahyo Sahara, Ana Setyo Budhi Sitarina Widyarini Sri Hartati