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Dwi Priyowidodo
Departemen Parasitologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences Veterinary

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Journal : Jurnal Veteriner

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Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.
Identifikasi Cacing Trematoda dan Gambaran Patologi Ginjal Burung Merpati yang Terinfeksi (IDENTIFICATION AND PATHOLOGICAL FEATURES OF TREMATODE IN PIGEON’S KIDNEY) Ana Sahara; Joko Prastowo; Dwi PriyoWidodo; Eryl Sri Rohayati; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study is conducted to identify species of trematode and its pathological features in pigeons’kidney. Twenty five of Yogyakarta pigeons were examined for trematode infection in kidneys. One ofkidneys was mashed in mortar with a little water, the other was examined for histopathological featuresstained with hematoxyline-eosin.Trematodes found were stained with Schmison ´s Carmine. Seven pigeons(28%) were infected by trematode with non significant clinical features. Identification trematodescharacterized by oral sucker, pharynx, testes are slighty diagonal in position, irregular in shape and intracaecal. Ovary pretesticular and vitellaria widespread from anterior ovarium to the posterior body.Histopathological examination showed segment of trematode in the medullary collecting ducts lumen,dilatation, flattening and emptyness of ducts epithelial cells, emptyness, giant cells and dominationmononuclear cell in interstitial tissue, characterizing a granulomatous nephritis. Trematodes foundidentified as Paratanaisia bragai. The trematodes were found in very dilated medullary collecting ductsand caused inflammation in adjacent tissues. Further studies are needed to find out vector of trematodein pigeons .
Co-Authors . Dwilantiani Ana Sahara Ana Sahara Andhini Mutiara Putri Asmarani Kusumawati Awaludin, Aan Dana Meida Dyah Kunthi Wirapratiwi Eryl Sri Rohayati Gifti Rosalina Ratnaningrum Guntari Titik Mulyani Hilda Syara Shita Devi Ida Tjahajati Joko Prastowo Kurnia Kurnia Lily Gunawan Nabilah Putroe Agung Nugraheni, Yudhi Ratna Raden Wisnu Nurcahyo Sahara, Ana Setyo Budhi Sitarina Widyarini Sri Hartati