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Endang Listyaningsih S
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Journal : Nexus Biomedika

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Pengaruh Pemberian Ekstrak Daun Sambiloto (Andrographis paniculata [Burm.F.] Ness) terhadap Kerusakan Struktur Histologis Sel Hepar Mencit yang Diinduksi Parasetamol Putri, Heigy Mutiha; Listyaningsih S, Endang; Riyadi, Slamet
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Green chiretta contains andrographolide as active substances, flavonoids, saponins, and tannins which were thought to protect the liver’s cell  from free radicals. In present study, Green chiretta leaves extract was evaluated for its protective effect on heksaklorosiklon-induced liver cell damage in mice. Methods: To evaluate the hepatoprotective effect of Green chiretta leaves extract, 28 male, Swiss Webster mice (Mus musculus) were equally divided into four groups. The group I (KK) were administered aquadest and group II (KP1) were not only administered aquadest but also a toxic dose of paracetamol on the day 12, 13 and 14. Grup III–IV (KP2-KP3) were administered Green chiretta leaves extract (0.2 ml and 0.4 ml, successively) for 14 days and a toxic dose of paracetamol on the day 12, 13 and 14. Mice were sacrificed on the 15th day with neck dislocation and their livers were prepared for microscopy study to evaluate their cell damages. The  damages were assessed  by  counting  the  number  of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. The data were analyzed using the One Way ANOVA test (α = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results: The mean liver cell damage score of  the KP1 group was significantly higher  (61.80 ± 7.991) than the KK group (33.00 ± 5.225). The KP2 group showed fewer numbers of liver cell damage (37.81 ± 5.653) compared to the KP1 group (61.80 ± 7.991) and the KP3 group (60.81 ± 8.268). The One Way ANOVA test showed the significant difference between the four groups (p=0.000) while the  LSD test showed the significant differences between each other group (p<0.005), except the group of KP1-KP3 (p> 0.005). Conclusions: The treatment of Green chiretta leaves extract was able to decrease the liver cell damage of mice. The increase dose of Green chiretta leaves extract was not followed by the increase of protection effect to the liver cell damage of mice induced by paracetamol. Keywords: Green chiretta leaves extract, paracetamol, liver cell histological damage.  
Pengaruh Pemberian Ekstrak Daun Sambiloto (Andrographis paniculata [Burm.F.] Ness) terhadap Kerusakan Struktur Histologis Sel Hepar Mencit yang Diinduksi Parasetamol Heigy Mutiha Putri; Endang Listyaningsih S; Slamet Riyadi
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Green chiretta contains andrographolide as active substances, flavonoids, saponins, and tannins which were thought to protect the livers cell from free radicals. In present study, Green chiretta leaves extract was evaluated for its protective effect on heksaklorosiklon-induced liver cell damage in mice. Methods: To evaluate the hepatoprotective effect of Green chiretta leaves extract, 28 male, Swiss Webster mice (Mus musculus) were equally divided into four groups. The group I (KK) were administered aquadest and group II (KP1) were not only administered aquadest but also a toxic dose of paracetamol on the day 12, 13 and 14. Grup IIIIV (KP2-KP3) were administered Green chiretta leaves extract (0.2 ml and 0.4 ml, successively) for 14 days and a toxic dose of paracetamol on the day 12, 13 and 14. Mice were sacrificed on the 15th day with neck dislocation and their livers were prepared for microscopy study to evaluate their cell damages. The damages were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. The data were analyzed using the One Way ANOVA test (? = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results: The mean liver cell damage score of the KP1 group was significantly higher (61.80 7.991) than the KK group (33.00 5.225). The KP2 group showed fewer numbers of liver cell damage (37.81 5.653) compared to the KP1 group (61.80 7.991) and the KP3 group (60.81 8.268). The One Way ANOVA test showed the significant difference between the four groups (p=0.000) while the LSD test showed the significant differences between each other group (p<0.005), except the group of KP1-KP3 (p> 0.005). Conclusions: The treatment of Green chiretta leaves extract was able to decrease the liver cell damage of mice. The increase dose of Green chiretta leaves extract was not followed by the increase of protection effect to the liver cell damage of mice induced by paracetamol. Keywords: Green chiretta leaves extract, paracetamol, liver cell histological damage.
Pengaruh Pemberian Ekstrak Buah Naga Putih (Hylocereus undatus)Terhadap Struktur Histologis Paru Mencit (Mus musculus) Yang Dipapar Asap Rokok Nurul Wahda Aulia; endang listyaningsih s; yulia sari .
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: White Dragon fruit contains substances like vitamin A, E, C and polyphenolic compound that can counteract free radical. Cigarette smoke is a type of oxidant compound that causes oxidative stress and lung damage. The purpose of this study was to determine the effect of White Dragon fruit extract on histological of the alveolar lung of mice exposed by cigarette smoke, and to find out whether the increase in dose enhanced the protective effect against histological of the alveolar lung exposed by cigarette smoke. Methods: This study was a laboratory experimental research with the post-test only control group design. The subjects used in this experiment was male Swiss Webster mice (Mus musculus), 2-3 months old and 20g of body weight. Sampling technique was incidental sampling. Twenty eight mice were divided into 4 groups, each group consisted of 7 mice. Negative control group / KK(-) was administered distilled water. Positive Control Group/ KK(+) was administered distilled water, then, 2 hours later was treated with cigarette smoke. Treatment group 1 (KP1) and 2 (KP2) were administered white dragon fruit extract with dose 70 mg/20 g and 140 mg/20 g, successively, then, 2 hours later were treated with cigarette smoke. These treatments were performed for 14 days. On the day 15, all lungs of subjects were prepared for histological study. Lung damage was assessed by looking at the destruction of the alveolar septum, pulmonary edema, and infiltration of inflammatory cells. Observation result were classified into normal category, moderate and serious. Then, the data were analyzed using the Kruskal-Wallis test and Mann Whitney test with ? = 0.05. Results: The Kruskal-Wallis test showed that at least one group had significantly greater effect than other groups with p = 0.004 (p <0.050). The Mann Whitney test showed significant difference between KK(-) KK(+), KK(-) - KK2, KK(+) - KK1 groups and no significant difference between KK(-) KK, KK(+) - KK2, KK1 KK2 groups. Conclusions: White Dragon fruit extract provided protective effect against mices lung histological damage from cigarette smoke. The increasing dose of White Dragon fruit extract did not enhance the protective effect of histological lung structure of the mice against cigarette smoke. Keywords:White Dragon fruit, cigarette smoke, histological damage of lung.
Co-Authors Asaduddien Faras Heigy Mutiha Putri Lilik Wijayanti Nurul Wahda Aulia Sigit Setyawan Siti Marufah Slamet Riyadi Sri Haryati Sugeng Purnomo Widia Susanti Yulia Sari yulia sari .