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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) International Journal of Renewable Energy Development
Tony Liwang
Department of Biotechnology, Plant Production and Biotechnology Division, PT.SMART Tbk Jl. Raya Cijayanti Kampung Pasirmaung-Sentul West Java 16810, Indonesia
Agriculture, Biological Sciences & Forestry Chemistry Economics, Econometrics & Finance Energy

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Optimization of Concentration and EM4 Augmentation for Improving Bio-Gas Productivity from Jatropha curcas Linn Capsule Husk G.A, Praptiningsih; Hendroko, Roy; Wahono, Satriyo K; Sasmito, Andi; O. Nelwan, Leopold; Nindita, Anggi; Liwang, Tony
International Journal of Renewable Energy Development Vol 3, No 1 (2014): February 2014
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.3.1.73-78

Abstract

Most literature suggests that two-phase digestion is more efficient than single-phase. The series of two-phase digestion studies have been conducted from 2011 to 2013 at the research farm of PT Bumimas Ekapersada, West Java, Indonesia. This paper reports on a research on optimation of concentration and augmentation of EM-4 (effective microorganism-4), a local commercial decomposer, as efforts to stabilize a biogas technology which made from husk capsules of Jatropha curcas Linn (DH-JcL). The studies of increasing organic loading rate (OLR) for the two-phase digestion was conducted to improve efficiency.  The concentration variable studied was 1: 8 (1 part DH-JCL and 8 parts water), compared to 1: 12 as a control. The augmentation treatment is the addition of EM-4 by 5% (v/v). It was also examined the augmentation of F2-EM4 (150 times duplication of EM-4) due to cost consideration. The studies were conducted in the laboratory which using a liter and two liters of glass digester and glass wool as immobilized growth. The results of this study support the previous studies: the optimum concentration was 1: 8, EM-4 was able to increase biogas production in two-phase digestion, yet biogas production decrease at single-phase. F2-EM4’s ability to support production of biogas were equivalent to that of EM-4.
Identification of Gene Related to Hard Bunch Phenotype in Oil Palm (Elaeis guineensis Jacq.) ,, Roberdi; ,, Sobir; Yahya, Sudirman; Toruan-Mathius, Nurita; Liwang, Tony
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.814 KB) | DOI: 10.24831/jai.v43i2.10421

Abstract

ABSTRACTMolecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon
Isolasi dan Karakterisasi Potongan DNA Gen Sterol Metiltransferase 1 (SMT1) Asal Kelapa Sawit Rizal, Syamsi; Sukma, Dewi; Siberakuno, Roberdi; Liwang, Tony; Sudarsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.715 KB) | DOI: 10.24831/jai.v48i3.32205

Abstract

Tanaman dengan pertambahan tinggi yang lambat merupakan karakter penting dalam program pemuliaan kelapa sawit dan brasinosteroid merupakan hormon penting yang berpengaruh untuk karakter tersebut. Gen sterol metiltransferase 1 (SMT1) merupakan salah satu gen kunci dalam biosintesis brasinosteroid. Pengembangan marker molekuler dapat dimulai dengan identifikasi dan karakterisasi gen yang terkait dengan karakter target. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi keragaman nukleotida gen SMT1. Amplifikasi PCR dilakukan menggunakan genom Elaeis oleifera (E.o.), E. guineensis (E.g.) dan hibrida dari keduanya (E.g. x E.o.) dengan sepasang primer spesifik gen SMT1. Dalam penelitian ini berhasil diamplifikasi satu potongan DNA gen SMT1 dan dari hasil DNA sequencing berhasil diidentifikasi sebanyak 501- 505 pb. Potongan DNA yang diidentifikasi terdiri atas partial intron 8, exon 9, intron 9, dan patial exon 10, yang menyandi antara 104-105 residu asam amino. Domain residu asam amino terkonservasi ditemukan dalam hasil translasi amplikon, yang berkorelasi dengan situs aktif dalam biosintesis brasinosteroid dan mengkonfirmasi identitas amplikon sebagai bagian dari gen SMT1. Keragaman nukleotida potongan DNA gen SMT1 yang teridentifikasi berpotensi dapat digunakan untuk pengembangan marker molekuler yang bermanfaat untuk pemuliaan tanaman kelapa sawit, terutama untuk karakter pertambahan tinggi tanaman yang lambat. Kata kunci: Brasinosteroid, sterol, tanaman kerdil
Co-Authors Ahmad Wahyudi Andi Sasmito Dewi Sukma Leopold O. Nelwan Nindita, Anggi Nurita Toruan-Mathius Praptiningsih G.A Praptiningsih G.A. Rizal, Syamsi Roberdi ,, Roberdi Roy Hendroko Salafudin . Salundik . Satriyo K Wahono Satriyo K. Wahyono Siberakuno, Roberdi Sobir Sobir Sudarsono Sudirman Yahya