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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia
Nurita Toruan-Mathius
Plant Production and Biotechnology Div., TCLZ and Biotechnologi Lab., PT SMART Tbk, Jl. Raya Pajajaran 78 F-6, Bogor
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Medicine & Pharmacology

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Formulasi Trichoderma dan Mikoriza Arbuskular untuk Pengendalian Penyakit Busuk Pangkal Batang pada Kelapa Sawit: Formulation of Trichoderma sp. and Arbuscular Mycorrhiza to Control Basal Stem Rot Disease in Oil Palm Sinthya E, Hana Christine; Khairani, Hagia Sophia; Sinaga, Meity Suradji; Toruan-Mathius, Nurita; Nawangsih, Abjad Asih
Jurnal Fitopatologi Indonesia Vol. 20 No. 6 (2024): November 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.6.286-294

Abstract

Basal stem rot (G. boninense) is one of the most important diseases in oil palm. As biocontrol agents, Trichoderma spp. and arbuscular mycorrhizal fungi (AMF) have been reported to inhibit the growth of G. boninense. Nevertheless, the effectiveness of those biocontrol agents is unstable due to less appropriate formulation and application. Biofungicide formulation affects the effectiveness in controlling plant diseases, the ability to proliferate and survive in the environment, the period of storage, and reduces preparation time and application cost. This research aimed to develop the formulation of Trichoderma spp., and AMF to control G. boninense. Trichoderma spp. The active ingredient has been mass-produced in millet formulated with carriers: T1 using rice bran, while T3 was combined with rice bran and oil palm empty fruit bunches (EFB) compost. AMF active ingredient has been mass producted in shorgum root, and formulated with carrier, CMA1 using zeolite. While, CMA2 combined zeolite with EFB compost. AMF applications were on seed planting, Trichoderma spp. applications were on three months seedlings. Plant material used was DXP (76 × 36.11), which is susceptible to G. boninense. Disease progress was observed from latent period, disease incidence, disease severity, infection rate (r), area under disease progress curve (AUDPC), and the AMF-Trichoderma association with plant oil palm root. CMA2 and T3 formulation showed the best result to reduce disease severity and insidence. Therefore, CMA2 + T3 was the best combination of formulation for controlling oil palm basal stem rot disease based on disease progress.
Keragaman Genetik Kelapa Sawit (Elaeis guineensis Jacq.) Asal Angola Menggunakan Marka SSR Sayekti, Urip; Widyastuti, Utut; Toruan-Mathius, Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.461 KB) | DOI: 10.24831/jai.v43i2.10420

Abstract

ABSTRACTEffort to increase productivity and other elite characters in Indonesia oil palm breeding program is facing a problem because of the narrow genetic diversity. To broaden the genetic diversity, germplasm exploration has been done in Angola, Central Africa. The objective of this research was to assess the genetic diversity and population structure of Angola originated oil palm germplasm based on 20 SSR markers. The plant materials used were 27 accessions consisted of 136 palms planted in Riau, Sumatera. The DNA was isolated and amplified using PCR. Phylogeny analysis was constructed using Unrooted Neighbor-Joining by DARwin software 6.0.8. The result showed that polymorphic information content (PIC) value is 0.55 (0.17 to 0.75 for each locus) with 102 total number of alleles. Genetic diversity between individuals was higher compared to the genetic diversity within accessions or regions and between accessions or regions. Phylogenetic analysis of 27 accessions showed that accessions were divided into three main groups. Every group containing individuals originated from 5 spatial distribution regions. Principal coordinates analysis (PCoA) showed that accessions were distributed in one structure. Using more primers and samples to get more representative data is recommended for the following research.Keywords: allele, locus, germplasm, molecular marker, polymorphic
Identification of Gene Related to Hard Bunch Phenotype in Oil Palm (Elaeis guineensis Jacq.) ,, Roberdi; ,, Sobir; Yahya, Sudirman; Toruan-Mathius, Nurita; Liwang, Tony
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.814 KB) | DOI: 10.24831/jai.v43i2.10421

Abstract

ABSTRACTMolecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon
Co-Authors Hagia Sophia Khairani Meity Suradji Sinaga Nawangsih, Abjad Asih Roberdi ,, Roberdi Sinthya E, Hana Christine Sobir Sobir Sudirman Yahya Tony Liwang Urip Sayekti UTUT WIDYASTUTI