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S. Margino
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Pemurnian dan Karakterisasi Enzim Endokitinase dari Agen Pengendali Hayati Trichoderma reesei Harjono Harjono; S. M. Widyastuti; S. Margino
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 2 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9594.734 KB) | DOI: 10.22146/jpti.10074

Abstract

This experiment was aimed to purify and characterize the endochitinase of Trichoderma reesei. Extracellular endochitinase was produced by T. reesei strain T13, a fungal biocontrol agent in colloidal chitin medium as sole carbon source. The enzyme was purified by ammonium sulfate precipitation, followed by gel filtration chromatography and chromatofocusing. The results showed that T. reesei produced endochitinase with molecular weight of 32 kDa and the activity was optimum at pH of 5,5 and temperature of 30 to 35oC.
Isolasi dan Karakterisasi Β-1,3-Glukanase Akar Semai Tusam (Pinus merkusii Jungh. et de Vriese) yang Berasosiasi dengan Fungi Ektomikorisa M. D. Anggoro; S. M. Widyastuti; S. Margino
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12086

Abstract

Association between tusam (Pinus merkusii Jungh. et de Vriese) seedling roots with ectomycorrhiza fungi was expected to increase the activity {β-1,3-glucanase level in the plants. This enzyme has potency to protect seedling from soil borne fungal pathogens by degrading the fungal cell walls. The objectives of this research were to isolate and characterize β-1,3-glucanase from tusam seedling roots associated with ectomycorrhiza fungi. Crude protein was isolated with ammonium sulfat precipitation and then purified by gel filtration chromatography and characterize molecular weight, temperature and pH for optimum the activity. The enzyme from tusam seedling roots associated with ectomycorrhizal fungi, designated as GLUC15 have 15 kD molecular weight, 30° - 40° C temperature and pH 5-7 for optimum activity.
Isolasi dan Karakterisasi Kitinase Akar Tusam (Pinus merkusii Jungh. et de Vriese) Roots yang Bersimbiosis dengan Fungi Ektomikoriza A. Handayani; S. M. Wisyastuti; S. Margino
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12099

Abstract

The experiment was aimed to detect the activity and characterize the chitinase of tusam root during ectomycorrhizal symbiosis. Tusam inoculated with tusam stands soil from Kaliurang as fungi inocula. Crude proteins were isolated from 4, 6, and 8 weeks age of tusam. Chitinase activity and isoform were detected using glycol chitin as a subtrat. The enzyme was purified by ammonium sulfat precipitation, dialysis, followed by gel filtration chromatography. The results showed that tusam prodced chitinasse with a molecular weight of approximately 52kDa. The optimum activity was at pH 5 and temperature of 30°C.
Co-Authors A. Handayani Harjono Harjono M. D. Anggoro S. M. Widyastuti S. M. Wisyastuti