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All Journal Jurnal Perlindungan Tanaman Indonesia
M. D. Anggoro
Balai Konservasi Sumber Daya Alam Kalimantan Timur
Agriculture, Biological Sciences & Forestry

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Isolasi dan Karakterisasi Β-1,3-Glukanase Akar Semai Tusam (Pinus merkusii Jungh. et de Vriese) yang Berasosiasi dengan Fungi Ektomikorisa M. D. Anggoro; S. M. Widyastuti; S. Margino
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12086

Abstract

Association between tusam (Pinus merkusii Jungh. et de Vriese) seedling roots with ectomycorrhiza fungi was expected to increase the activity {β-1,3-glucanase level in the plants. This enzyme has potency to protect seedling from soil borne fungal pathogens by degrading the fungal cell walls. The objectives of this research were to isolate and characterize β-1,3-glucanase from tusam seedling roots associated with ectomycorrhiza fungi. Crude protein was isolated with ammonium sulfat precipitation and then purified by gel filtration chromatography and characterize molecular weight, temperature and pH for optimum the activity. The enzyme from tusam seedling roots associated with ectomycorrhizal fungi, designated as GLUC15 have 15 kD molecular weight, 30° - 40° C temperature and pH 5-7 for optimum activity.
Co-Authors S. M. Widyastuti S. Margino