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All Journal Jurnal Perlindungan Tanaman Indonesia
Dewa Gede Wiryangga Selangga
Faculty of Agriculture, Warmadewa University Jln. Terompong No. 24, Tanjung Bungkak, Denpasar, Bali 80239 Indonesia
Agriculture, Biological Sciences & Forestry

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Detection of Strawberry vein banding virus (SVBV) and Identification of Viruliferous Insects Associated with Strawberry Plants (Fragaria sp.) in Bali I Putu Sudiarta; Gusti Ngurah Alit Susanta Wirya; Dewa Gede Wiryangga Selangga; Made Getas Pudak Wangi
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.57714

Abstract

Plant pests and diseases cause decreases in strawberry yield in Bali, including plant viruses. Strawberry vein banding virus (SVBV) is a virus that infects strawberry plants and is transmitted by insects. However, studies about this disease in Bali are still limited. This study aimed to detect SVBV within insect bodies and determine the insect species of its vector. Methods used included (1) sampling; (2) detection of SVBV from insect bodies using primers (SVBV F/SVBV R) and PCR; and (3) molecular identification of viruliferous insect with primers (LCO 1490 F/HCO 2198 R) using PCR and sequencing analysis. This study successfully detected SVBV DNA in an insect from three insect families associated with strawberry plants in Pancasari Village, Buleleng Regency, Bali. Results from the nucleotide sequences analysis in SVBV viruliferous insects indicate that the insect was Myzus persicae.
Molecular Identification of Sweet potato virus C on Sweetpotato in Bali, Indonesia Listihani Listihani; Dewa Gede Wiryangga Selangga
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.64545

Abstract

A survey was conducted in several sweet potato cultivations in Bali Province. Survey found that many plants exhibited potyvirus symptom, such as chlorosis blotches. This study was to determine disease incidence, detection and identification of the virus causing these symptoms on sweet potato plants in Bali. Samples were collected by purposive sampling of 10 plants from each location in Bali (Denpasar, Gianyar, Badung, Buleleng, Tabanan, Klungkung, Karangasem, Jembrana, Bangli). Disease insidence was observed based on viral symptoms in the field. Identification of nucleic acids was done using Potyvirus universal primer and DNA sequencing. Disease incidence in Bangli, Buleleng, and Denpasar Regencies was > 50%. RT-PCR and CiFor/CiRev Potyvirus universal primers successfully amplified ± 700 bp of CI genes from all samples from Bangli, while samples from 8 other districts were not amplified using the same primers. The SPVC isolate of sweet potato showed nucleotide and amino acid homology similarities with the sweet potato isolate from East Timor (MF572066), 96.8% and 97.4%, respectively and these were referred to the "Asian" strain. This indicates that SPVC has spread in East Java and Bali.
The Existence of Papaya ringspot virus-Papaya Strain on Cucumber in Gianyar, Bali Dewa Gede Wiryangga Selangga; I Ketut Widnyana; Listihani Listihani
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.64703

Abstract

Yellow mosaic symptoms were identified from cucumber production systems in Gianyar and were similar to symptoms of PRSV infection. Further research was conducted to determine diseases incidence and molecular characteristic of PRSV. Ninety leaf samples were collected from Gianyar by purposive sampling and disease incidence calculations were based on symptoms in the field. Detection and identification were done using a RT-PCR with specific primers of CP PRSV-P, CP PRSV-W and DNA sequencing. Disease incidences in the fields ranged between 5.81–66.87%. Specific DNA band 470 bp was successfully amplified from several cucumber leaf samples collected from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh; but no DNA were amplified from all samples when using CP PRSV-W specific primer. Nucleotide and amino acid analysis showed nucleotides homology to isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh, i.e. 98.9–99.5% and 99.1–100%, respectively. Results indicated that genetic variation of PRSV-P from Gianyar was low. Furthermore, the nucleotides homology of PRSV-P isolates from Ubud, Payangan, Tegallalang, Sukawati, Gianyar, and Blahbatuh were with PRSV-P isolates which infected cucumbers from Nganjuk (LC311783) and Brebes (LC311784), while from native papaya collected in Bali Bali (LC223115) were 97.2–98.4% and 98.6–100%, respectively. Phylogenetic analysis confirmed that PRSV-P isolates from Indonesia were in the same cluster with Thailand isolates. The results showed that sources of PRSV-P inoculums spreading into new areas.
Co-Authors Gusti Ngurah Alit Susanta Wirya I Ketut Widnyana I Putu Sudiarta Listihani, Listihani Made Getas Pudak Wangi