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All Journal Journal of Tropical Biodiversity and Biotechnology Penbios: Jurnal Pendidikan Biologi dan Sains
Dewi Yuliana Rizqi
Biology Education Department, State Islamic University Of Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Public Health

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Growth of Kaffir Lime (Citrus hystrix DC) Cell Line Derived from Seed Explant After Yeast Elicitation Using Pure and Technical Grade Yeast Dewi Yuliana Rizqi; Frisca Damayanti; Fhea Putri Cristy; Alisa Julia Nurulita; Aries Bagus Sasongko; Endang Semiarti; Woro Anindito Sri Tunjung
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 1 (2022): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68650

Abstract

The addition of elicitors in kaffir lime (Citrus hystrix DC.) culture is one of  strategies for obtaining and increasing the production of secondary metabolites.  Saccharomyces cerevisiae is one of the elicitors that can be used to increase secondary metabolites such as terpenoids. However, in its use, the pure cultures of S. cerevisiae are expensive. Therefore, the first objective of this study was to analyze the ability of technical grade (commercial baker’s yeast) to be used as an elicitor and measure the growth of kaffir lime cell line after being elicited by pure and technical grade (commercial baker’s yeast). The second objective is to determine the best time to subculture kaffir lime cell line after elicitation. We observed the morphology and measured the growth curve of pure and technical grade yeast until the 4th subculture generation. Furthermore, we used both grades of yeast for elicitation. Kaffir lime cell suspension was treated with 10 ppm pure grade or 5 ppm and 10 ppm technical grade yeast for 4 days. After elicitation, kaffir lime cell lines were subcultured and their growth was analyzed. The result showed that the morphology and growth curve of technical grade until 4th subculture generations was similar to the pure grade. On the other hand, after elicitation using pure and technical grade yeast and being subcultured, the growth of the elicitated kaffir lime cell line had the same pattern as the control group, but the cell density of the control group was higher than the elicitated group. The initial stationary phase of kaffir lime cell line was on the 17th day which is the best time to subculture. The subculturing process is important to maintain the viability of the kaffir lime cell line. 
Isolation and Identification of Hydrocarbon Degrading Bacteria From Used Lubricant Oil Risa Umami; Dewi Yuliana Rizqi
PENBIOS: JURNAL PENDIDIKAN BIOLOGI DAN SAINS Vol 6 No 01 (2021): PENBIOS: Jurnal Pendidikan Biologi dan Sains, Volume 06 No 01 May 2021
Publisher : Biology Education Department, Faculty of Teacher Training and Education, University of Nahdlatul Wathan Mataram - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/penbios.v6i01.636

Abstract

Hydrocarbons are organic compounds consisting of carbon and hydrogen. Petroleum in particulary lubricant oil is a group of hydrocarbon compounds which can be grouped into aliphatic, alicyclic, and aromatic hydrocarbons. The use of oil as a lubricant will leaving used lumbricant oil that if not managed properly will cause environmental pollution. This research aims to isolate and identifying of hydrocarbon degrading bacteria from used lubricant oil. Type of research is descriptve explorative with qualitative approach. Isolation is done by adding the sample of used lubricant oil into the NA medium (Natrient Agar) by observing the clear zone formed. Bacterial identification is done by observing morphology and biochemical activity. Four isolates were obtain and two of them were selected to identification are isolate I and III. The result from bacterial identification was performed based on morphological and biochemical characterization show isolate I is Bacillus brevis and isolate III is Bacillus macerans.
Co-Authors Alisa Julia Nurulita Aries Bagus Sasongko Endang Semiarti Fhea Putri Cristy Frisca Damayanti Risa Umami Woro Anindito Sri Tunjung