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All Journal Quagga Indonesian Journal of Biotechnology Biota Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Jurnal Biologi Tropis Jurnal Pertanian Agros Jurnal Ilmiah Ilmu Terapan Universitas Jambi
Aries Bagus Sasongko
Faculty Of Biology Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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The growth improvement of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet using photoautotrophic micropropagation system Aries Bagus Sasongko; Asruwaidah Fatumi; Ari Indrianto
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1351.142 KB) | DOI: 10.22146/ijbiotech.27167

Abstract

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.
PENGARUH WARNA CAHAYA LIGHT-EMITTING DIODES (LED) INTENSITAS RENDAH DAN CEKAMAN DINGIN TERHADAP PERTUMBUHAN VEGETATIF ANGGREK Phalaenopsis HIBRIDA Dzikrina Nurunisa; Aries Bagus Sasongko; Ari Indrianto
Biota Vol 4 No 1 (2018): Jurnal Biota 2018
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v4i1.1683

Abstract

Cahaya merah dan biru merupakan cahaya utama yang diserap tanaman dan berperan penting terhadap fotosintesis, pertumbuhan serta perkembangan tanaman anggrek Phalaenopsis hibrida. Cekaman dingin mampu memicu pembentukan organ generatif dan memperlambat pertumbuhan organ vegetatif. Namun, pengaruh intensitas cahaya rendah dan cekaman dingin terhadap pertumbuhan vegetatif dan generatif anggrek Phalaenopsis belum diketahui. Penelitian ini bertujuan untuk mengetahui pengaruh warna cahaya intensitas rendah dan cekaman suhu dingin terhadap pertumbuhan vegetatif anggrek Phalaenopsis hibrida. Anggrek berumur ± 2 tahun ditumbuhkan dalam growth chamber dengan penyinaran lampu LED putih sebagai kontrol (100 lx), merah, biru, dan merah + biru (masing-masing 30 lx) sebagai perlakuan. Perlakuan suhu air terbagi menjadi cekaman dingin (4,5oC), suhu ruang (28oC) dan kontrol negatif (tanpa penyiraman). Daun diukur panjang, jumlah daun, densitas stomata selama 60 hari. Hasil penelitian menunjukkan bahwa cahaya LED intensitas rendah dapat meningkatkan ukuran panjang daun, namun cahaya merah memberikan hasil pertambahan panjang daun tertinggi. Perlakuan cahaya LED biru memberikan densitas stomata tertinggi terhadap warna cahaya lainnya. Di sisi lain, cekaman dingin menghambat pertumbuhan daun. Berdasarkan analisis ANOVA dan Tes Duncan, diperoleh bahwa antar setiap perlakuan baik warna cahaya dan suhu air tidak berbeda nyata. Warna cahaya dan suhu air tidak berpengaruh terhadap jumlah daun maupun pembukaan stomata daun.
Implementasi Inovasi Budidaya Stroberi di Agrowisata Banyuroto Kabupaten Magelang Melalui Education for Sustainable Development Ganies Riza Aristya; Aries Bagus Sasongko; Lisna Hidayati; Agus Setiawan
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1190.266 KB) | DOI: 10.22146/jpkm.26500

Abstract

The cultivation of strawberry needs of technological innovation to gain competitive advantage strawberry fruit quality and quantity. This is motivated because the decline in the production of strawberries in the area and quality of strawberry fruit is still low. Since 2012 until now, Laboratory of Genetics has identified and developed the character of phenotype and genotype of strawberry plants in Agro Banyuroto, Village Banyuroto Sawangan District of Magelang regency. Character genotypes studied through the identification of ploidy with cytogenetic approach and apply poliploidisasi technology that aims to double the number of sets of chromosomes in order to obtain a plant that has the character of superior phenotype compared with the control. The purpose of this activity was to awaken the capacity of communities that were able to develop and utilize natural resources to meet human needs while maintaining the environmental implementing action plans that lead to sustainable development in a sustainable manner. The method was executed in this activity was the cultivation of strawberries by exploiting natural resources Banyuroto village environment that was using bamboo, strawberry cultivation techniques with vertikultur systems. Moreover, the carrying capacity was also supported by the promotion of village tourism as central region Banyuroto strawberry cultivation of strawberries superior to tourists every week, promotion through Online system (in the network) by utilizing Information Technology and Computing and community empowerment with the diversification of food made from strawberries. The end result of this activity was obtained hallmarks of crops polyploidy were plant growth faster, the ability of the formation of stolons and nursery faster, leaf area and stem diameter was wider, the stem stout and roots were longer, the fruit produced was greater and a sweeter taste with more red fruit color. Innovation strawberry crop is expected to be followed by the cultivation of sustainable optimal as possible so that the results obtained are able to improve the welfare of farmers in Banyuroto strawberries.
Innovation of Natural Orchid Cultivation Technology for Tourism Development in Banyunganti Hamlet, Jatimulyo Village, Girimulyo Sub-District, Kulon Progo District, Yogyakarta Endang Semiarti; Aziz Purwantoro; Ari Indrianto; Aries Bagus Sasongko; Oktaviana Herawati; Asri Fajar Milasari
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.46283

Abstract

Orchid is the best tourism icon which focused on nature-based tourism development in Indonesia. Banyunganti Hamlet is one of the tourism villages in Kulon Progo which has a high diversity of orchid species. Regarding this situation, guiding and assistance for villagers which is focused on the introduction of natural orchid species and its character, conventional propagation, and modern propagation by using household-scale tissue culture techniques (sowing seeds and planting) is important to give. The long-term goal for this activity was for empowering women in Dusun Banyunganti in line with the opening of the New Yogyakarta International Airport by the government.
Growth of Kaffir Lime (Citrus hystrix DC) Cell Line Derived from Seed Explant After Yeast Elicitation Using Pure and Technical Grade Yeast Dewi Yuliana Rizqi; Frisca Damayanti; Fhea Putri Cristy; Alisa Julia Nurulita; Aries Bagus Sasongko; Endang Semiarti; Woro Anindito Sri Tunjung
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 1 (2022): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68650

Abstract

The addition of elicitors in kaffir lime (Citrus hystrix DC.) culture is one of  strategies for obtaining and increasing the production of secondary metabolites.  Saccharomyces cerevisiae is one of the elicitors that can be used to increase secondary metabolites such as terpenoids. However, in its use, the pure cultures of S. cerevisiae are expensive. Therefore, the first objective of this study was to analyze the ability of technical grade (commercial baker’s yeast) to be used as an elicitor and measure the growth of kaffir lime cell line after being elicited by pure and technical grade (commercial baker’s yeast). The second objective is to determine the best time to subculture kaffir lime cell line after elicitation. We observed the morphology and measured the growth curve of pure and technical grade yeast until the 4th subculture generation. Furthermore, we used both grades of yeast for elicitation. Kaffir lime cell suspension was treated with 10 ppm pure grade or 5 ppm and 10 ppm technical grade yeast for 4 days. After elicitation, kaffir lime cell lines were subcultured and their growth was analyzed. The result showed that the morphology and growth curve of technical grade until 4th subculture generations was similar to the pure grade. On the other hand, after elicitation using pure and technical grade yeast and being subcultured, the growth of the elicitated kaffir lime cell line had the same pattern as the control group, but the cell density of the control group was higher than the elicitated group. The initial stationary phase of kaffir lime cell line was on the 17th day which is the best time to subculture. The subculturing process is important to maintain the viability of the kaffir lime cell line. 
Induksi Kalus Piper retrofractum Vahl. dengan Variasi Eksplan dan Zat Pengatur Tumbuh Asyroful Muna; Suharyanto Suharyanto; Aries Bagus Sasongko
Quagga : Jurnal Pendidikan dan Biologi Vol 14, No 1 (2022): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v14i1.3796

Abstract

Cabe Puyang merupakan tanaman yang berpotensi untuk dikembangkan menjadi tanaman obat di Indonesia. Salah satu hambatan pengembangan tanaman ini adalah tingkat produktivitasnya yang rendah. Metode yang dapat digunakan untuk meningkatkan produktivitas Cabe Puyang adalah kultur kalus. Penelitian ini bertujuan untuk mengetahui apakah kultur kalus merupakan metode yang efektif untuk perbanyakan Cabe Puyang. Eksplan daun dan batang diinokulasi pada medium Murashige and Skoog (MS) dengan penambahan 0,5 mg/l 2,4-D dan 0,5 mg/l BA untuk mengetahui eksplan paling responsif. Eksplan tersebut diinokulasi pada medium MS dengan kombinasi 2,4-D:BA dan NAA:BA (0:0; 0,5:0,5; 0,5:1; dan 1:0,5) mg/l untuk optimasi medium induksi kalus. Kalus disubkultur ke medium dengan penambahan BA (0; 0,5; 1; 2; dan 3) mg/l untuk optimasi medium regenerasi tunas. Hasil penelitian menunjukkan eksplan batang lebih responsif dibandingkan daun. Kombinasi 0,5 mg/l 2,4-D + 0,5 mg/l BA dan 0,5 mg/l 2,4-D dan 1 mg/l BA menunjukkan respon pembentukan kalus paling cepat, yaitu selama 9 hari. Belum terdapat tunas yang terbentuk pada tahap regenerasi, namun pada konsentrasi 1 mg/l BA kalus mulai menunjukkan tanda regenerasi, seperti tekstur kalus friable dan warna menjadi kuning kehijauan.Javanese chili is a plant that has the potential to be developed into medicinal plants in Indonesia. One of the obstacles of the development of this plant is the low level of productivity. The method that can be used to increase the productivity of Long pepper is callus culture. This study aims to determine whether callus culture is an effective method for Long pepper propagation. Leaf dan stem explants were grown on Murashige dan Skoog (MS) medium with the addition of 0.5 mg / l 2,4-D dan 0.5 mg / l BA to determine the most responsive explants. The explants were grown on MS medium with a combination of 2,4-D: BA dan NAA: BA (0: 0; 0.5: 0.5; 0.5: 1; dan 1: 0.5) mg / l for optimization of callus induction medium. Callus was sub cultured to the medium with the addition of BA (0; 0.5; 1; 2; dan 3) mg / l for the optimization of the shoot regeneration medium. The results showed stem explants were more responsive than leaves. The combination of 0.5 mg / l 2,4-D + 0.5 mg / l BA dan 0.5 mg / l 2,4-D dan 1 mg / l BA showed the fastest response to callus formation, which was for 9 days. No shoots were formed at the regeneration stage, but at a concentration of 1 mg / l BA callus began to show signs of regeneration, such as a friable callus texture dan a greenish yellow color.
INDUKSI PEMBUNGAAN IN VITRO PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Blume INDONESIA IN VITRO FLOWERING OF INDONESIAN Phalaenopsis amabilis (L.) Blume Ixora Sartika Mercuriani; Agus Slamet; Bekti Sulistya Utami; Aries Bagus Sasongko; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Agros Journal of Agriculture Science Vol 16, No 2: Edisi Juli 2014
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.529 KB)

Abstract

Bunga adalah faktor yang sangat penting dalam budidaya anggrek. Salah satu kendala yang sering dijumpai dalam budidaya anggrek adalah lama fase vegetatif yang dibutuhkan tanaman tersebut untuk dapat berbunga. Pada penelitian ini induksi pembungaan dilakukan pada tahap pertumbuhan awal tanaman secara in vitro. Tanaman umur enam bulan setelah tanam (bst) disubkultur pada medium New Phalaenopsis (NP) dengan konsentrasi Nitrogen (N) setengah dari NP (1/2NP), dengan atau tanpa pemberian Benzyl Adenine (BA), variasi konsentrasi KH2PO4 (1,5 mM dan 3 mM), serta dengan atau tanpa pemotongan akar. Kombinasi perlakuan dengan pemberian BA 22.2 µM, kandungan P tinggi (3 mM), dan pemotongan akar mampu mempercepat pembungaan in vitro anggrek Phalaenopsis amabilis (P. amabilis) asli Indonesia pada umur 18 bst.Flowering is very important in orchid cultivation. However, the long vegetative phase to be able to bloom of the plant becomes an important problem. The orchid needs three up to five years after sowing to bloom. In this study, flowering induction is done in the early growth stages of plants. At six months after sowing (mas), plants were sub-cultured on New Phalaenopsis (NP) medium witha half Nitrogen(N) concentration of NP (1/2NP), with or without Benzyl Adenine (BA), concentration variations of Phosphor/P (1,5 mM and 3 mM),  and with or without roots cutting. In vitro flowering of Indonesian Phalaenopsisamabilis (P. amabilis) can induced on medium that contain 22.2 µM BA and 3 mM P with roots cutting at 18 mas.
Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke Fajri Marisa; Lisna Hidayati; Aries Bagus Sasongko; Tri Rini Nuringtyas
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2629

Abstract

Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.
In Vitro Shoot Induction of Musa acuminata cv. Mas Kirana Raden Roro Rifka Annisa; Arkan Setiaji; Aries Bagus Sasongko
Quagga : Jurnal Pendidikan dan Biologi Vol 13, No 1 (2021)
Publisher : Program Studi Pendidikan Biologi, FKIP Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v13i1.3431

Abstract

Abstract: The availability of ‘mother plants’ used for source of explants is one of the most important limitations for the development of in vitro culture in new banana cultivar’s. To increase produce large quantities of uniform and healthy seedlings, induction of multiplication has been carried out on Musa acuminata cv. Mas Kirana. Plant regeneration were obtained by culturing sucker on MS medium supplemented with PGRs BAP and NAA (1 mg/l + 0.5 mg/l, 2 mg/l + 0.5 mg/l, and 3 mg/l + 0.5 mg/l) and TDZ (0.25 mg/l, 0.5 mg/l, and 0.75 mg/l). The highest shoot formation was found in 0.25 mg/l TDZ with average shoots of 13.67 ± 3.16 and primary shoot height is 20 cm. TDZ induction tends to form somatic embryos, while induction of BAP and NAA leads to organogenesis. The rate of contaminants occurrence from bacteria is 87.50% and 12.50% from fungus. In histological observation, TDZ, BAP, and NAA affect the development of many new meristematic zones seen in the scalps.
EFFECTIVENESS OF ANTIOXIDANTS AS BROWNING INHIBITORS AGAINST SHOOT Cyrtostachys renda AS CALLUS CULTURE EXPLANT Syamsurizal Syamsurizal; Diah Tri Utami; Elisma Elisma; Lizawati Lizawati; Aries Bagus Sasongko; Beni Hendro Prabowo
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 9 No. 1 (2025): Volume 9, Nomor 1, March 2025
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v9i1.36002

Abstract

Cyrtostachys renda has various pharmacological activities. C. renda roots contain alkaloids and flavonoids as secondary metabolites' main components responsible for anti-inflammatory and anticancer activities. To produce secondary metabolites from C. renda, tissue culture can be used through callus and cell suspension culture techniques. Browning is one of the main challenges in plant tissue culture, which inhibits the success of callus formation, especially in plants with a high content of phenolic compounds such as C. renda. This study tested the effect of soaking in polyvinylpyrrolidone (PVP) and ascorbic acid (AA) in inhibiting the browning process in C. renda explants. Explant colour change testing was done in vitro by soaking the explants in five concentrations of PVP and AA (50, 100, 150, 200 and 250 ppm). Changes in explant colour were then analyzed using the Royal Horticultural Society (RHS) colour chart. The results showed that the PVP antioxidant with a concentration of 200 ppm could suppress explant browning until the fourth day. In comparison, explants treated with AA antioxidants with a concentration of 200 ppm showed browning symptoms on the second day. It can be concluded that antioxidants can reduce the browning level in C. renda shoot explants, and the antioxidant PVP can reduce the browning level better than AA. The mechanism of PVP as an anti-browning agent through inhibition of phenolic oxidation. This study's novelty is obtaining the type of anti-browning PVP with an optimum concentration of 200 ppm to inhibit the browning of C. renda shoot explants.
Co-Authors Agus Setiawan Agus Slamet Alisa Julia Nurulita Ari Indrianto Ari Indrianto Ari Indrianto Arkan Setiaji Asri Fajar Milasari Asruwaidah Fatumi Asyroful Muna Aziz Purwantoro Bekti Sulistya Utami Beni Hendro Prabowo Dewi Yuliana Rizqi Diah Tri Utami Dzikrina Nurunisa Elisma Elisma Endang Semiarti Fajri Marisa Fhea Putri Cristy Frisca Damayanti Ganies Riza Aristya Ixora Sartika Mercuriani Kuni Faizah Lisna Hidayati Lizawati Lizawati Maemonah, Maemonah Nova Avisha Dewi Oktaviana Herawati Raden Roro Rifka Annisa Sukarti Moeljopawiro Surodjo Taat Andayani Swandari, Tantri syamsurizal syamsurizal Tri Rini Nuringtyas Woro Anindito Sri Tunjung