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All Journal Berkala Ilmiah Pendidikan Biologi (BioEdu) Matematika dan Sains AGRIVITA, Journal of Agricultural Science
Mahanani Tri Asri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Languange, Linguistic, Communication & Media Mathematics

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EFEKTIVITAS Spodoptera Litura Multiple Nukleopolyhedrosis Virus) YANG DIPAPARI DENGAN SINAR ULTRAVIOLET-C DAN SINAR MATAHARI TERHADAP KEMATIAN Spodoptera litura Fabr. Mahanani Tri Asri, ; Sri Kentjananingsih, ; Yuni Kristiningsih,
Matematika dan Sains Vol 16, No 2 (2009)
Publisher : Matematika dan Sains

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Abstract

The propose of research was to compare the effectivity of UV-exposed and sunray-exposed SpLtMNPVs against the mortility of S. litura larvae. This experiment is designated in laboratory with completely randomized design for differentiating the effectivity of SpLtMNPV which have been 12, 16, 24 hours sunray exposed and 8 hours UV-C lamp to the mortality of S. litura F. larvae. There were 6 repetition for each group with the SpLtMNPV concentration as much as 107 PIBs/ml, so there are 24 experiment units. Each treatment includes 10 3rd instar of S. litura F. larvae. The results of research showed that sunray exposure has decreased the effectivity of SpLtMNPV viruses faster than UV-C exposure. This was showed through the fewer S. litura larvae has been killed by sunray exposed viruses than by the U.V. exposed viruses. On the 8th day application, there was only 52 - 55% larvae killed by sunray exposed viruses compared to 78% larvae killed by UV exposed viruses.rd instar of S. litura F. larvae. The results of research showed that sunray exposure has decreased the effectivity of SpLtMNPV viruses faster than UV-C exposure. This was showed through the fewer S. litura larvae has been killed by sunray exposed viruses than by the U.V. exposed viruses. On the 8th day application, there was only 52 - 55% larvae killed by sunray exposed viruses compared to 78% larvae killed by UV exposed viruses.
EFEKTIVITAS SPODOPTERA LITURA MULTIPLE NUKLEOPOLYHEDROSIS VIRUS (SlMNPV) HASIL PERBANYAKAN PADA SEL LINE UNTUK MENGENDALIKAN LARVA Spodoptera litura SKALA GREEN HOUSE DENGAN BERBAGAI WAKTU APLIKASI Mahanani Tri Asri, ; Nur Ducha,
Matematika dan Sains Vol 17, No 2 (2010)
Publisher : Matematika dan Sains

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The biological control is the best choice to control plant pest population. SlMNPV is the one kind of the biological control agent which effective to control the Spodoptera litura caterpillar. The caterpillar attacked to many kind of agrocultural crops like Ricinus comunis, soybean, cotton, and tobacco. The effectivenes SlMNPV reproduce cresult in cell line of to control the caterpillar in green house in various concentrations. This research conducted to know the effect of virus concentration ( 7,1 x 107 PIBs/ml, 7,1 x 108 PIBs/ml, and 7,1 x 109 PIBs/ml) to control the caterpillar (Spodoptera litura) death in three application times (the morning; 06.00; day :12.00 and evening: 16.00). The results of the research showed that the virus concentration of 7,1 x 108PIBs/ml PIBs/ml effective to control caterpillar population because the caterpillar death reached 80% (biological control standart) and the best time to applicate was evening.7 PIBs/ml, 7,1 x 108 PIBs/ml, and 7,1 x 109 PIBs/ml) to control the caterpillar (Spodoptera litura) death in three application times (the morning; 06.00; day :12.00 and evening: 16.00). The results of the research showed that the virus concentration of 7,1 x 108PIBs/ml PIBs/ml effective to control caterpillar population because the caterpillar death reached 80% (biological control standart) and the best time to applicate was evening.
SENYAWA FLAVANON DARI TUMBUHAN Suyatno, ; Nurul Hidajati, ; Mahanani Tri Asri, ; Rizka Ayu Hamida,
Matematika dan Sains Vol 17, No 2 (2010)
Publisher : Matematika dan Sains

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Abstract

A flavanone namely 5-hydroxy-7-methoxy flavanone (pinostrobin) had been isolated from dichloromethane extract of the leaves of the Pteriss vittata. It was gained as a pale yellow needles, m.p. 96-97 oC. The molecular structure of isolate was identified by spectroscopic methods, including UV, IR, and EIMS.A flavanone namely 5-hydroxy-7-methoxy flavanone (pinostrobin) had been isolated from dichloromethane extract of the leaves of the Pteriss vittata. It was gained as a pale yellow needles, m.p. 96-97 oC. The molecular structure of isolate was identified by spectroscopic methods, including UV, IR, and EIMS.
PENGEMBANGAN E-LKPD VIRUS BERBASIS PROBLEM BASED LEARNING UNTUK MELATIHKAN KETERAMPILAN BERPIKIR KRITIS SISWA KELAS X Dwi Rahmawati; Mahanani Tri Asri
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol 12 No 1 (2023)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

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Pandemi COVID-19 mengubah sistem pendidikan di Indonesia menjadi sistem pembelajaran online. Sebagai pendidik, guru harus mampu memahami situasi dan menyiapkan bahan ajar yang cocok agar pembelajaran dapat terus berjalan secara optimal. Salah satu bahan ajar yang cocok digunakan saat pembelajaran online yaitu E-LKPD. Penelitian ini bertujuan untuk menghasilkan bahan ajar berupa E-LKPD virus berbasis Problem Based Learning (PBL) untuk melatih keterampilan berpikir kritis siswa kelas X yang valid, praktis dan efektif. Penelitian ini menggunakan metode penelitian pengembangan dengan model 4-D yang terdiri atas Define, Design, Develop dan Disseminate. Parameter yang diukur yaitu validitas, keefektifan dan kepraktisan. Validitas E-LKPD dinilai menggunakan instrumen lembar validasi yang dilakukan oleh dosen ahli materi, dosen ahli pendidikan dan satu guru Biologi SMA. Keefektifan diukur menggunakan hasil pretest dan post-test yang dianalisis dengan N-gain score serta analisis ketercapaian aspek berpikir kritis. Sedangkan kepraktisan E-LKPD didapatkan melalui hasil respon siswa menggunakan angket respon siswa. Hasil validasi E-LKPD memperoleh persentase sebesar 89,25% yang dikategorikan sangat valid. Hasil keefektifan memperoleh N-gain score sebesar 0,84 dengan kriteria tinggi dan ketercapaian aspek berpikir kritis dengan persentase 94,18% yang dikategorikan sangat kritis. Sedangkan kepraktisan mendapatkan persentase 99% dengan kategori sangat praktis. Dari hasil penelitian dapat disimpulkan bahwa E-LKPD virus berbasis PBL untuk melatih keterampilan berpikir kritis dinyatakan sangat valid, praktis dan efektif sebagai bahan ajar untuk kelas X SMA. Dengan menggunakan E-LKPD virus berbasis PBL dapat melatihkan dan meningkatkan keterampilan berpikir kritis siswa, serta mengetahui penguasaan siswa terhadap materi virus. Selain itu pengembangan E-LKPD juga bisa diterapkan pada materi yang lain. Kata Kunci: E-LKPD, virus, Problem Based Learning, keterampilan berpikir kritis.
MECHANISM OF INFECTION Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) ON MIDGUT EPITHELIAL CELL ARMY WORM (Spodoptera litura) Observed by TEM Mahanani Tri Asri; Siti Rasminah Chaelani; Bambang Tri Rahardjo; Sutiman Bambang Sumitro
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.115

Abstract

Spodoptera litura is one of  agricultural crop pests. They are resistant to chemical insecticides. One of arternate biological control is Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) Penelitian ini dilakukan untuk mengetahui bagaimana mekanisme SpLtMNPV dalam menginfeksi sel epithel midgut larva S. The research was conducted to determine how SpltMNPV infected midgut epithelial cells of S. litura secara in vitro . litura larvae in vitro.  The mechanism of infection was observed by a transmission electron microscopy (TEM).  The result of this observation showed that infecting midgut cell by  SpltMNPV involved 5 phases, they are, 1) the attachment of  SpltMNPV at the membrane of suitable host, 2) the penetration, formation of channels and release of protein envelope, 3) the biosynthesis of virus components in the cell nucleus, 4) the assembling of virus components, and 5) the releasing of MNPV/multiplenucleocapsid through budding. Keywords: mechanisms of infection, Spodoptera litura multiple nucleopolyhedrosis virus, midgut army worm larvae cells.
Co-Authors Bambang Tri Rahardjo Dwi Rahmawati Guntur Trimulyono Isnawati Lukas S. Budipramana Nur Ducha Nurul Hidajati Rizka Ayu Hamida Siti Rasminah Chaelani Sri Kentjananingsih Sutiman Bambang Sumitro Suyatno Yuni Kristiningsih