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All Journal Dentino: Jurnal Kedokteran Gigi Denta: Jurnal Kedokteran Gigi
Arlette Setiawan
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Dentistry

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BIOACTIVE COMPOUNDS OF RASAMALA (ALTINGIA EXCELSA NORNHA) LEAVES AS C-MYC PROTO ONCOGENE EXPRESSION SUPPRESSOR OF HUMAN TONGUE CANCER CELL IN VITRO Risyandi Anwar; Arlette Setiawan; Supriatno Supriatno; Unang Supratman
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5379

Abstract

Background: Tongue cancer is a common neoplasm found in oral cavity. It is characterized by aggressive cell growth, poor prognosis and being the cause of mortality. Objectives: to discover bioactive compounds of Rasamala leaves which possess an activity to inhibit SP-C1 human tongue cancer cell proliferation by reducing the expression of c-Myc proto oncogene. Methods: This is an experimental laboratory study using SP-C1 human tongue cancer cell. Separation of bioactive compounds from Rasamala leaves ethyl acetate extract was using various chromatography techniques guided by antiproliferative assay. Results: Two compounds were produced consisting of kaempferol (1) and quercetin (2). Compound 1 and 2 were tested to assess antiproliferative activity of kaempferol and quercetin upon SP-C1tongue cancer cell. IC50 values obtained from antiproliferative assay of each compound were 0.72 and 0.70 ug/ml respectively. Data analysis using ANCOVA test attained a significant value of α=0.05 and proceeded for probit analysis. The activity of  compound 1 and 2 was tested on c-Myc proto oncogene and it was acquired that compound 1 and 2 can suppress c-myc proto oncogene expression. Conclusion: Rasamala compounds consist of kaempferol (1) and quercetin (2) which possess an activity as tongue cancer cell proliferation inhibitor by reducing c-myc proto oncogene expression.
Kaempferol and Quercetin Isolated from The Leaves of Atingia Excelsa to Arrest Cell Cycle in G0/G1 Phase Human Tongue Cancer SP-C1 Cell Lines Risyandi Anwar; Arlette Setiawan; Supriatno; Unang Supratman
Denta Journal Kedokteran Gigi Vol 14 No 1 (2020): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i1.4

Abstract

The leaves of Altingia excelsa were found to strongly inhibit SP-C1 human tongue cancer cell lines. This study was focused on identifying the antiproliferative compound found in A. excelsa leaves and assessing its mechanism of action. The active compound was isolated using column chromatography and identified by the spectroscopic method and was also tested for its anti-proliferative properties and the cell cycle analysis in SP-C1 cells by flowcytometry analysis. This work resulted in the isolation of a flavonoid, which was identified to be kaempferol and quercetin. The compounds inhibited SP-C1 cell proliferation in a time- and dose-dependent manner with IC50 values of 0.72 µg/mL and 0. 70 µg/mL for the 24 hours treatments, respectively. Furthermore, the flowcytometry analysis suggested that the compounds exerted its anticancer activities by inhibiting cell cycle. These results suggested that compounds found in A. excelsa providies a basis for its potential use in cancer disease management.
Co-Authors Risyandi Anwar Supriatno Supriatno Supriatno Unang Supratman