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Agung Suprihadi
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OPTIMASI PEMBERIAN PUPUK GRAMAFIX DALAM DEGRADASI CEMARAN MINYAK BUMI OLEH BAKTERI INDIGENOUS SECARA IN VITRO Linda Safitri; Agung Suprihadi; Endang Kusdiyantini; Yeti Darmayati
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Petroleum pollution has bad impact for life in marine ecosystems, particularly in coastal areas. One method of handling the pollution is bioremediation using bacteria that are able to degrade and utilize petroleum hydrocarbons as carbon source. One technique of bioremediation is biostimulation, that is the addition of nutrients that can improve the process of oil degradation by degrading bacteria. Nutrient slow-release fertilizer are used, one of which is Gramafix. The purpose of this study was to determine the optimal concentration of Gramafix in increasing degradation of petroleum contaminants by indigenous bacteria. Four concentrations of Gramafix as treatments are P1 (0,085 g), P2 (0,171 g), P3 (0,341 g) and P4 (0,682 g), as well as the negative control treatment (no fertilizer and bacteria) and positive control (with bacteria, without fertilizer). Observations were carried out  four times, on 0, 7, 14 and 28 days of incubation. The parameters used are heavy oil using the gravimetric method, the total number of bacterial cells using Acridine Orange Direct Counting and environmental factors such as nitrogen contents, phosphorus contents, temperature, dissolved oxygen, pH and salinity. Analysis of the data using a completely randomized design (CRD) with ANOVA and Duncan test. The results showed the P3 can improve the process of oil degradation by bacteria that degrade the highest percentage of 65,91% in the 28 days of incubation, it also has more bacterial cells than other treatments. The result of this study is the addition of 0,341 g Gramafix is optimum for enhancing the degradation of petroleum hydrocarbons by degrading bacteria. Keywords: Pollution, petroleum, bioremediation, biostimulation, slow-release fertilizer.
POTENSI RHIZOBAKTERI DARI TANAMAN KUBIS (Brassica oleracea var. capitata L.) DAERAH GETASAN SEMARANG SEBAGAI AGEN BIOBAKTERISIDA TERHADAP PATOGEN Xanthomonas campestris Maya Fitriana Ilul Fahmi; Anto Budiharjo; Agung Suprihadi
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria are bacteria that live around plant roots but do not cause negative effects on their host and are known to act as a biobactericide agent. The use of chemical-based pesticide against plant pathogen can be replaced with rhizobacteria. The objectives of this research were to isolate rhizobacteria from cabbage, explored from Getasan, Semarang  and to determine the ability of the isolates to inhibit pathogens Xanthomonas campestris causing black rot on cabbage in vitro. The research concisted of  rhizobacterial isolation, morphologic bacterial characterization, antibacterial test, and molecular and biochemical identification of the isolates. The isolation obtained seventeen rhizobacterial isolates. Four isolates (K.1, K.3, K.9 and K.12) showed potency as an biobactericide agent against pathogenic X. campestris. K.9 had the best ability to inhibit the growth of X.campestris by 12,6 mm. Based on molecular identification K.9 was Bacillus cereus strains BF15. Morphology and Biochemistry test showed that isolates K.9 is gram positive bacteria shaped bacilli, able to form an endospore, positive in hydrolysing starch , fermentation glucose, motile, aerobic and negative in the fermentation of manitol and arabinosa. Keywords : Antibacterial, Rhizobacteria, Cabbages, black rot disease, Xanthomonas. campestris
UJI KEMAMPUAN PRODUKSI SITOKININ OLEH RHIZOBAKTERI Tri Wijiastuti; Agung Suprihadi; Baedah Rupaedah
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Plant growth regulators have a role in the process of growth and development of a plant. One of the Plant growth regulators which play an important role in process of growth and development is cytokinin. Cytokinin are able to stimulate division and cell enlargement, accelerate formation of organs, delay aging of various types of plants, and increase number and size of leaves. The utilization of rhizobacteria which is capable in producing cytokinin as a basic component of biological fertilizer has a very important role in improving the growth of plants. This research aims to select the capability of rhizobacteria isolates which are able to produce cytokinin and determine morphological characteristics and biochemical characteristics of potential rhizobacteria isolates as a cytokinin producer. The research was conducted at the Laboratory Agromikrobiologi, Biotechnology Research Center, Agency for the Assessment and Application of Technology, Puspitek, Serpong. The research procedure was rhizobacteria cultivation and extraction of cytokinin from rhizobacteria culture, then detect cytokinin using the method of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The results showed that the isolates which were capable to producing cytokinin was Jember 2.2 with a concentration of 92 mg L-1 and expected as Pseudomonas genus bacteria. Keywords : biofertilizers, rhizobacteria, cytokinin.
ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI PESTISIDA DICOFOL DARI TANAH SAWAH DI KABUPATEN KARAWANG Anindita Ayu Pratiwi; Agung Suprihadi; Budi Raharjo; Priyo Wahyudi; Sih Parmiyatmi
Jurnal Akademika Biologi Vol. 1 No. 1 Oktober 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Karawang was the center of rice production, but rice production in paddy fields in Karawawang often faces obstacle hopper pest that caused crop failure. These condition prompted the farmers to using dicofol pesticides excessively, thus caused pollution of pesticides. The purpose of this research were get the isolates bacteria that could degrade the pesticide dicofol in Karawang paddy fields and discovered characteristics of bacteria that have potential to degrade pesticides. The research was conducted with the isolation and characterisation of bacteria from Karawang paddy soil, the characterisation of isolates bacteria by observation morphological and phenotypic include staining Gram and biochemical tests, test isolates bacteria resistence of dicofol, measuring the growth curve and High Performance Liquid Chromatography (HPLC) for discovering of decreased dicofol concentration during 24, 48, and 72 hours of incubation time. The result is six isolates, that are AA1, AA2, AA3, AA4, AA5, and AA6. Isolates AA1 is suspected as genus Phenylobacterium, isolates AA3 and AA6 are suspected as genus Pseudomonas, AA4 isolates is suspected as genus Bacillus, isolates AA5 is suspected as genus Alcaligenes, but isolates AA2 not yet be identified. The results indicate that isolate AA6 has most resistent to dicofol and most number of cells bacteria, that is 7.9x108. Isolate AA6 is also the most effective in degrading dicofol as much as it can reduce the concentration in amount of 76.0 ppm (84.45%) in MSM medium + 90 ppm dicofol. Keywords : Isolation, Characterisation, Bacteria, Resistention, Degradation, Dicofol Pesticide
KEANEKARAGAMAN DAN AKTIVITAS ENZIMATIS KAPANG RIZOSFER KACANG MEONGAN (Aeschynomene americana L.) DI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA Griffin Natassya; Agung Suprihadi; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Molds are widely distributed in nature, they even present in extreme environment, such as hot and dry soil. Molds which can grow in extreme environment has been adapted to xeric environment by producing enzymes  with  special  characteristics.  The  aim  of  this  study  to  determine  the  diversity  of  molds  from A.americana L. rhizosphere at West Sukolilo Village, Labang district, Bangkalan Regency, Madura and also to examined their cellulolytic and proteolytic activities. Isolation of molds were done by using spread and dilution methods. Molds identification were done by macroscopic and microscopic examined. The molds diversity was calculated using Shannon-Wiener index. Semiquantitative enzyme examination were done using Carboxymethil Cellulose (CMC) agar for cellulolytic ang liquid gelatin 12% for proteolytic. Results of this study showed that mold diversity isolated from   A. americana L. rhizosphere was moderate (1,8-2,7) with total of 43 species, comes from 7 genus i.e. Aspergillus, Penicillium, Fusarium, Aureobasidium, Byssochlamys, Paecilomyces, and Trichoderma.  The  highest  index  of  cellulolytic  produced  by  Aspergillus  sydowii  (2,35),  while  highest proteolytic activity produced by Aspergillus flavus (86%). Keywords : molds, diversity index,xeric, cellulolytic, proteolytic
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI “TEMPOYAK” Arina Aisyah; Endang Kusdiyantini; Agung Suprihadi
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Tempoyak is a food made from fermented durian flesh with the addition of salt and brooded for seven days. Tempoyak quality is strongly influenced by the presence of microbes involved during the process of fermentation. This study aimed to isolate and characterize lactic acid bacteria (LAB) that play a role in the tempoyak fermentation process, and to perform proximate analysis of the durian and tempoyak. Isolation was done on 0, 3rd, 5th, and 7th day fermentation with streak methods. The isolates then underwent morphology and motility observation, biochemical tests, and proximate analysis.  The isolation of tempoyak resulted seven isolates of bacteria that had different colony and cell morphology. Gram staining of the bacterial cell produced a purple color with the rod and spherical shape. Motility test resulted non motile bacteria. Catalase test of bacteria isolates produced negative catalase. Isolates showed positive results in test for acid production and carbohydrate fermentation. pH decrease from 6.88 to 5.74 on the last day of brooding. Seven isolates obtained had characteristics that were similar with the characteristic of LAB, which was Gram positive, rod shaped or spherical, non motile, negative catalase, and produce acid. Tempoyak nutrient contained, ie 15.12% moisture content, 27.03% ash content, 2.69% fat content, 6.37% protein content, and 48.79% carbohydrate content. Keywords: tempoyak, LAB, morphology, biochemistry, proximate
PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN JAGUNG (Zea mays) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Debby Widiyanti; Sri Pujiyanto; Agung Suprihadi; Mamik Setyowati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Maize (Zea mays) is the second most important food commodity after rice plants. The role of the corn crop in agricultural commodities encourage the use of biological fertilizers to increase crop productivity of maize. The use of biological fertilizers open opportunities using rhizosphere bacteria as inoculants boosters grow plants.This study aims to obtain bacterial isolates from the rhizosphere of maize (Zea mays), characterize the morphology and biochemical properties of the ability to produce IAA, provide nutrients such as N and P and to get a bacterial inoculant boosters grow corn. Rhizosphere bacteria were isolated using SEA media (soil extract agar). Isolates that have the ability to produce IAA, tether N and high P solvents tested against corn seed germination and applied in a phase of plant growth. The results showed there were 24 isolates corn crop rhizosphere dominated by gram-positive bacteria and shaped basil capable of producing IAA grading 4,83 to 125,84 ppm. 16 rhizosphere bacterial isolates capable of dissolving phosphate with phosphate solubility index ranged from 2,1 to 4,6. 18 rhizosphere bacterial isolates capable of tether N with the highest N tether capacity of 2,8 x 10 3 CFU/ mL. Application 3 selected bacteria such as J11, J16 and J19 as inoculants are not able to increase the growth of corn plants compared to control water. Keyword :Maize, Rhizosphere, The growth of corn plants.
PERTUMBUHAN ISOLAT RHIZOBAKTERI PELARUT FOSFAT DARI TANAMAN PADI DI MAYONG, JEPARA PADA MEDIA LIMBAH RUMAH PEMOTONGAN HEWAN DAN AIR KELAPA Rutty Wulandari; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The farmers are now turning towards the use of biofertilizer. The biofertilizer is living microbes applied to the soil in order to help facilitate or provide certain nutrients for plants. Previous study found that B4 isolate has proven capable of solubilizing phosphate so that it could be used as an agent  that was inoculated in biofertilizer. The fertilizer can be formulated by modifying the alternative growth media in the liquid form that has potential, which is slaughterhouse waste and coconut water. Both  media contain organic matters which support the growth of bacteria. This study aimed to test the potential of slaughterhouse waste and coconut water as a medium for the growth of B4 isolate. Methods  of research conducted by Randomized Block Design, in which consists of five treatments namely P1 (100% coconut water), P2 (25% slaughterhouse waste & 75% coconut water), P3 (50% slaughterhouse waste & 50% coconut water), P4 (75% slaughterhouse waste & 25% coconut water), and P5 (100% slaughterhouse waste). The measured variable was the number of bacteria in the organic waste media during the 48 hours incubation period. Data on the number of bacteria was analysed by ANOVA  test  then  continued  with  Duncan  and  LSD  further  tests.  The  results  showed  that P4 treatment significantly different from other treatments, with the highest number of bacteria (1,9 x 1011CFU/mL) at 18 hours incubation. The density of bacterial population in the five formula medias every 3 hours during the 48 hours incubation period showed significantly different, except at 0, 3, 12, and 45 hours incubation. Key words: biofertilizer, phosphate, slaughterhouse waste, coconut water
ISOLASI BAKTERIOFAG SPESIFIK Pseudomonas sp. DA1 DARI BIOFILM PADA SISTEM PENGISIAN AIR MINUM ISI ULANG. Zikra Hayati; Siti Nur Jannah; Agung Suprihadi
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The social demand for drinking water increases both in urban and rural areas. The state encourages the development of Industrial Water Supply Depot (DAM) refills. problems occurred is no standard method for treating drinking water is sterileand lack of government supervision. This raises the issue of sanitation that is the presence of pathogenic bacteria that form biofilms in drinking water refill system. One of the bacterial pathogen is Pseudomonas. Bacteria Pseudomonas at refill drinking water can cause infections in the digestive tract. This study aims to find specific bacteriophage isolates of biofilm samples to infect bacteria Pseudomonas sp. on refill drinking water system. Isolation host Pseudomonas done in 2 ways dilution and filtration and grown on selective media Pseudomonas Isolation Agar. Isolates obtained were then characterized by gram staining and Kligler Iron Agar (KIA) test. Isolation of bacteriophages of biofilm done by bacteriophage amplification and filtration to obtain filtrate bacteriophage I and II. Test performed by using the host's infection Pseudomonas sp. DA1, Pseudomonas aeruginosa and Salmonella sp. (LIPI’s collection). The results showed that the drinking water refill depot contained positive isolates of Pseudomonas is Pseudomonas sp. DA1 and obtained isolate specific bakteriofag that can infect Pseudomonas sp. DA1. Bacteriophage titer calculation results on each sample at 3,0 x 105 PFU/ml in drinking water depot, 3,3 x 107 PFU/ml in the water product, and 9 x 107 PFU/ml water sources. Keyword: refill drinking water systems, biofilms, Pseudomonas sp DA1, bacteriophage
ISOLASI, ENUMERASI DAN DETEKSI MOLEKULER GEN ToxR PADA BAKTERI Vibrio parahaemolyticus DARI TAMBAK UDANG VANNAMAE DI REMBANG Thu'ti Alawiyyah; Anto Budiharjo; Agung Suprihadi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The presence of opportunistic pathogen bacteria in shrimp pond water is caused by 3 factor. There is environmental factor, shrimp condition and the existence of bacteria. Vibrosis disesase in shrimp is harmfull to farmers because it makes reducing result and caused disease in humans. Bacteria often found among types of Vibrio. The toxR gene is known as the transmembrane regulator gene that control the expression of toxin gene. This study aims to determine the existence of toxR gene in Vibrio parahaemolyticus in pond water samples by PCR method. Bacterial isolation was performed using selective media CDVP Nissui. Enumeration of bacteria by TPC method to determine the number of suspected V. parahaemolyticus on CDVP. Extraction of bacterial DNA using QIAamp® DNA Mini Kit. A good qualitative result of the DNA extraction then amplified by toxR primer. Sequencing process sent to PT. Genetica Science. V. parahaemolyticus inoculated on CDVP media showed blue-green color because it can not ferment sucrose. Dry media CDVP Nissui can be used as a medium in the enumeration of Vibrio parahaemolyticus (1,6 x 102 CFU/ml in water sample) because it can be distinguished by the type of bacteria colony color that appears. The PCR protocol used was annealing at 62ºC with a cycle 30 times. Electrophoresis results showed that bacterial samples from pond water positively containing toxR gene with length 368 bp. From sequence base data of nucleotides, the sample has 99% equation with V. parahaemolyticus in the NCBI bank gene. Samples of V. parahaemolyticus bacteria in shrimp pond water in Rembang are known contain the toxR gene.Keyword : V. parahaemolyticus, toxR gene, enumeration, TCBS, CDVP
Co-Authors AB SUSANTO Anindita Ayu Pratiwi Aniza Rachmawati Anto Budiharjo Arina Aisyah Baedah Rupaedah Barri Pratama Berlian Abadianti Budi Raharjo Debby Widiyanti Doni Usman Endang Kusdiyantini Genoveva Preta Angelika Ghaida Afra Akhsani Griffin Natassya Kristiani Dwiatmi Laksono Trisnantoro Linda Safitri M Eka Prastya Mamik Setyowati Maya Fitriana Ilul Fahmi MG Isworo Rukmi Nabila Swarna Puspa Hermana Neny Nuraini Nur Sabrina Hasyyati Pramita Dian Pramitasari Prayogo, Fitra Adi PRIYO WAHYUDI Reza Auliarahman Bhaktinagara Roseliana Fitri Rutty Wulandari Sesaria Esa Sekar Ardini Sih Parmiyatmi Siti Nur Jannah Sona Fatimah Sri Pujiyanto Thu'ti Alawiyyah Tri Wijiastuti Tsania Dyna Falasifa w wijanarka Yeti Darmayati