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Agung Suprihadi
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Biochemistry, Genetics & Molecular Biology

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EKSPLORASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL SENYAWA ANTIFOULING YANG BERASOSIASI DENGAN ALGA COKLAT (PHAEOPHYTA) DI PERAIRAN KEPULAUAN KARIMUNJAWA JEPARA Barri Pratama; Endang Kusdiyantini; Agung Suprihadi; Anto Budiharjo; AB Susanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio
Biodegradasi Senyawa Hidrokarbon Oleh Strain Bacillus cereus(VIC) Pada Kondisi Salinitas Yang Berbeda Reza Auliarahman Bhaktinagara; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Bacillus cereus has been noted as hydrocarbonoclastic microbe that has ability to degrade hydrocarbons in non-saline conditions and some often to be found on high salinity environtment conditions. The purpose of this research is to determine the ability of strains Bacillus cereus (VIC) were isolated from non-saline environment to degrade hydrocarbons in crude oil on the different salinity condition. Bacillus cereus (VIC) was inoculated on the medium that has contaminated by crude oil with salinity level of 0,3 , 9,4 , dan 19,6 and incubated for 15 days. Determination of microbial growth is by using Total Plate Count (TPC) method along with determination of Total Petroleum Hydrocarbon (TPH) percentage using gravimetry method for every 5 day. The determination of microbial growth showed that Bacillus cereus (VIC) has the tolerancefor salinity level up to 19,6 because it is able to grow to a density of 6.9 x 106 CFU/ml on the 15th day. Bacillus cereus (VIC) is also able to degrades hydrocarbons on crude oil pollutants that indicated from degradation of TPH percentage in the medium up to 21% during 15 days incubation period on the medium with salinity level of 19,6 . Biodegradation using Bacillus cereus (VIC) can increase TPH degradation on the medium up to 19,8% than TPH degradation because of weathering. Keywords: Biodegradation, Bacillus cereus(VIC), Total Petroleum Hydrocarbon (TPH), Salinity.
IDENTIFIKASI SENYAWA BIOAKTIF PADA ISOLAT BAKTERI BUAH BELIMBING WULUH (Averrhoa bilimbi L.) SEBAGAI AGENSIA HAYATI Xanthomonas oryzae pv. oryzae Aniza Rachmawati; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bilimbi (Averrhoa bilimbi L.) is one of original fruit plants from Indonesia. Bilimbi fruits contain flavonoid, saponin, triterpenoid and tanin that have ability as anti-microbial. Bacteria was isolated from bilimbi fruits expected to be able produce bioactive compounds which can kill pathogenic bacteria such as Xanthomonas oryzae pv. oryzae which caused Bacterial Leaf Blight (BLB). Bacterial Leaf Blight in Indonesia caused harvest losses of 18 – 36 %. Infection was caused by X. oryzae caused the leaf symptoms to turn pale yellow, white, withered, and finally die. The purpose of this research are get bacteria isolate from bilimbi fruits (Averrhoa bilimbi L.) and testing bioactive compounds on bacteria isolate from bilimbi fruits which can inhibit Xanthomonas oryzae pv. oryzae (Xoo) growth. This research use methods, that are isolation of bacteria from bilimbi fruits and isolation of Xanthomonas oryzae pv. oryzae, characterization biochemically, inhibition test, and Thin Layer Chromatography. Obtained two bacteria isolate from bilimbi fruits. They are IBW1 dan IBW2, inhibition zona of IBW1 is 0,15 mm and IBW2 0,35 mm. Both of them have potential in antibacteria of X. oryzae bacteria eventhough in weak catagory. Metabolite secondary compound which play a role in antibacteria of X. oryzae is flavonoid compound. Kata kunci: antibacteria, bilimbi, Thin Layer Chromatography, X. oryzae
EKSPLORASI RHIZOBAKTERI INDIGENOUS TANAMAN CABAI RAWIT (Capsicum frustescens Linn.) DARI PERTANIAN SEMI ORGANIK DESA BATUR KABUPATEN SEMARANG SEBAGAI AGEN HAYATI PENGENDALI PERTUMBUHAN JAMUR Fusarium oxysporum f.sp capsici M Eka Prastya; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria is a group of bacteria that live in the around of plant roots. This type of bacteria known have the ability to stimulate plant growth by producing growth hormone, as well as to inhibit the growth of plant pathogens synthesize compounds with antibiotics or extracellular enzymes. The purpose of this study was to obtain and describe the morphological, biochemical and genetic isolates rhizobacteria of semi-organic farmland Semarang District village Batur which has the ability as a biological control agent of fungal pathogens Fusarium oxysporum f.sp capsici. The results obtained fifteen isolates the majority rhizobacteria bacilus shaped gram-positive and classified. Rhizobacteria inhibition test capabilities against pathogenic fungi was performed using dual culture test and test biomass. Dual culture test results showed that the inhibition of isolates E1 has a 3.77%, 1.88% isolates E3 and E15 isolates 22%. The biomass tests show E15 isolates capable of inhibiting the growth of pathogenic fungi best with the smallest weight of fungal biomass 0.0386 grams. The results of the molecular characterization based on 16S rRNA gene sequences to known that E15 isolates has similar with Bacillus cereus strain ATCC 14579 with similarity of 97%. The results of the biochemical characterization of isolates E15 has similarities with B. cereus species that is catalase positive, motile, have endospores, is able to hydrolyze starch and ferment glucose.Keywords: rhizobacteria, Fusarium oxysporum f.sp capsici, gen 16S rRNA, Bacillus cereus
UJI AKTIVITAS ANTIBAKTERI EKSTRAK TUMBUHAN Euphorbia hirta L. TERHADAP Ralstonia solanacearum, Escherichia coli, DAN Staphylococcus aureus SECARA IN VITRO Genoveva Preta Angelika; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biocontrol using Patikan kebo (Euphorbia hirta L.) plant is an alternative solution to control pathogenic bacteria. Such wild plant is known to contain active compounds with antibacterial activity such as tannins, alkaloids, flavonoids, saponins, and phenols. This study aims to determine the antibacterial activity of the methanol extract of E. hirta against R. solanacearum, E. coli and S. aureus. The extraction method of E. hirta was maceration with methanol solvent, while antibacterial activity test using the agar diffusion method (Kirby-Bauer) with test bacteria was R. solanacearum, E. coli and S. aureus. E. hirta extract tested was pure extract (100%). Observed response was diameter of inhibitory zone formed around the paper discs that had been smeared with E. hirta extract on the media. Analysis of the data using a Completely Randomized Design (CRD) 1 factor (test bacteria) with three times repetition, followed by a further test of Duncan with 95% confidence level. The results indicated that E. hirta produced extraction yield of 6,45%. Antibacterial activity of E. hirta extract against R. solanacearum, E. coli and S. aureus was indicated by Inhibitory Zone Diameter (HZD), respectively for 21,8 mm, 18,26 mm and 17,06 mm. The results of this study showed that the methanol extract of E. hirta plant had antibacterial activity against R. solanacearum, E. coli and S. aureus, thus can be used as a biocontrol agent of bacterial wilt disease in plants caused by R. solanacearum and human disease caused by E. coli and S. aureus. Keywords: Euphorbia hirta, Ralstonia solanacearum, Escherichia coli, Staphylococcus aureus, antibacterial activity, diffusion method
Produksi dan Kestabilan Pigmen Merah Kapang Monascus sp. Menggunakan Media Tepung Kulit Singkong dengan Penambahan Bekatul pada Konsentrasi yang Berbeda Sona Fatimah; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The used of synthetic dyes have a various of negative effects on human health. Pigment produced by Monascus sp. can be used as an alternative natural coloring food. The purpose of this research is to produce pigment Monascus sp. using the substrate cassava peels flour with the addition of rice bran at various concentrations. The study was conducted by growing Monascus sp. on medium cassava peels flour with the addition of rice  bran in various concentrations (0%, 2.5%, 5% and 10%). Measured parameter is the production of red pigment and pigment stability against temperature, water content, solubility and pH. Red pigment production was measured using a spectrophotometer with a wavelength of 500 nm. Analysis of the data using One Way ANOVA and Duncan test. The results showed that the highest production of red pigment (5.6 CVU / gds) and water content of 47% was obtained on medium cassava peels flour with rice bran addition of 10%. Pigment stability towards heat are 0,39 at 300C, 0,35 at 600C, 0,27 at 1210C, and 0,22 at 1500C , stability pH 3 are 0,37 (0 hours incubation) dan 0,26 (12 hours incubation) whereas pH 7 are 0,38 (0 hours incubation) dan 0,36 (12 hours incubation).Keywords: Fermentation,Cassava peels flour, Rice bran, Monascus, Pigment intensity, stability
Co-Authors AB SUSANTO Anindita Ayu Pratiwi Aniza Rachmawati Anto Budiharjo Arina Aisyah Baedah Rupaedah Barri Pratama Berlian Abadianti Budi Raharjo Debby Widiyanti Doni Usman Endang Kusdiyantini Genoveva Preta Angelika Ghaida Afra Akhsani Griffin Natassya Kristiani Dwiatmi Laksono Trisnantoro Linda Safitri M Eka Prastya Mamik Setyowati Maya Fitriana Ilul Fahmi MG Isworo Rukmi Nabila Swarna Puspa Hermana Neny Nuraini Nur Sabrina Hasyyati Pramita Dian Pramitasari Prayogo, Fitra Adi PRIYO WAHYUDI Reza Auliarahman Bhaktinagara Roseliana Fitri Rutty Wulandari Sesaria Esa Sekar Ardini Sih Parmiyatmi Siti Nur Jannah Sona Fatimah Sri Pujiyanto Thu'ti Alawiyyah Tri Wijiastuti Tsania Dyna Falasifa w wijanarka Yeti Darmayati