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Prayogo, Fitra Adi
Biomedical Sciences Study Program, Karya Husada University, Jl. R. Kompol Soekanto No.46, Semarang 50276, Indonesia
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MICROBIAL FUEL CELL (MFC) MENGGUNAKAN BAKTERI BACILLUS SUBTILIS DENGAN SUBSTRAT LIMBAH SEPTIC TANK SERTA PENGARUHNYA TERHADAP KUALITAS LIMBAH Fitra Adi Prayogo; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.858 KB)

Abstract

The power plant was still dependent on non-renewable energy is one factor in Indonesia fixed with the problems of electric energy crisis. Several lecturer have shown that B. subtilis can produced electricity through Microbial Fuel Cell (MFC). The purpose of this study is to measured the values of voltage, electric current, and power density as well as changes in the quality of waste water septic tank. Waste in a vacuum toilet can be of service "Doremon Jaya", sterilized and counted the value of the quality of wastes. B. subtilis inoculum was then inoculated into a bioreactor containing sterile waste septic tank with 4 different concentrations of 0%, 1%, 2% and 3% (v/v). Inoculum was incubated for 12 hours prior to observing. The result showed that B. subtilis with inoculum concentration of 3% (v/v) produced the greatest voltage, which is 299.70 mV on 4-hour with strong current and power density 0.2997 mA 8.98 mW / cm2. COD can reduced by up to 31.18% at the inoculum concentrations of 3% (v/v), while the value of BOD reduced by up to 25.03% at the inoculum concentrations of 3% (v/v). DO value also experienced the percentage decreased to 34.25% or decreased to 0.96 at the inoculum concentrations of 2% (v/v). pH value in the treatment of inoculum concentrations of 0%, 1%, 2% and 3% (v / v) decreased after the fermentation was consecutively 5.4, 4.8, 4.9, and 4.2  Kata kunci : Bacillus subtilis, Microbial Fuel Cell, Septic  tank, Wastewater quality
Revisit Study of Freshwater Sponges Eunapius carteri (Bowerbank, 1863) and a New Record of Oncosclera asiatica Manconi and Ruengsawang, 2012 (Porifera: Spongillida) in Porong River, East Java, Indonesia Setiawan Edwin; Yanuar Ahmad; Michael Einstein Hermanto; Riani Catur; Prayogo Fitra Adi; Budiharjo Anto
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.232-245

Abstract

Distribution of freshwater sponges (Porifera: Spongillida) in Indonesia is currently insufficiently reported and underestimated compared to marine sponges. An inventory study on freshwater sponges in East Java after Indonesian independence in 1945 is yet to be carried out. For this reason, we reported new records of two freshwater species, Eunapius carteri (Bowerbank, 1863) and Oncosclera asiatica (Manconi and Ruengsawang, 2012) in Porong river, East Java, Indonesia. E. carteri species was originally described in Mumbai, India and its presence in Java was reported in 1927, 1928, and 1930. DNA barcoding and molecular phylogenetic analysis using mitochondrial COI was able to successfully identify our specimens as E. carteri, while analysis using the nuclear ITS markers placed our samples with other Eunapius species. Furthermore, in this study we report for the first time in East Java, Indonesia, the presence of O. asiatica. The O. asiatica species was originally described from Pong River located in Lower Mekong, Thailand. We concluded our specimens to be O. asiatica based on the morphology, skeleton, and spicule composition of the specimens that were similar to the samples recorded in Thailand. We were only successful in obtaining the COI sequence of O. asiatica. Furthermore, our samples did not group to the publicly available sequence of its congener, namely Oncosclera sp. Therefore, further molecular taxonomy and morphological analysis is needed to explore the diversity of freshwater sponges in general and to conduct species delimitation of E. carteri and O. asiatica in Java, Indonesia, and Asia
Cloning and characterization of bgl6111 gene encoding β‐glucosidase from bagasse metagenome Fitra Adi Prayogo; Benjarat Bunterngsook; Pattanop Kanokratana; Hermin Pancasakti Kusumaningrum; Dyah Wulandari; Anto Budiharjo
Indonesian Journal of Biotechnology Vol 28, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.81536

Abstract

β‐Glucosidase (BGL) is an essential enzyme for the hydrolysis of cellulose in industrial processes, but natural BGL enzymes are poorly understood. Metagenomics is a robust tool for bioprospecting in the search for novel enzymes from the entire community’s genomic DNA present in nature. The metagenomics approach simplifies the process of searching for new BGL enzymes by extracting DNA and retrieving its gene information through a series of bioinformatic analyses. In this study, we report the gene cloning, heterologous expression of the bgl6111 gene (accession number MW221260) in Pichia pastoris KM71, and the biochemical characterization of the recombinant enzyme. We successfully identified the bgl6111 sequence of 2,520 bp and 839 amino acids with a molecular size of 89.4 kDa. The amino acid sequence of the bgl6111 gene showed 67.61% similarity to BGL from an uncultured bacterium (ABB51613.1). The BGL product has the highest activity on the third day at 1.210 U/mL, categorized as low production. The enzymatic activity could enhance up to 539.8% of 7.742 U/mL by using the ultrafiltration method. Our findings provide insightful information that bgl6111 obtained from bagasse metagenome could be an alternative candidate for industrial applications in the future.
Peningkatan Kualitas Produk melalui Diversifikasi Froozen Food untuk Balita Bagi UMKM Disekitar Rawa Pening Royhanaty, Isy; Amelia, Poppy Fransisca; Prayogo, Fitra Adi; Sari, Novita
Jurnal Peduli Masyarakat Vol 6 No 4 (2024): Jurnal Peduli Masyarakat: Desember 2024
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/jpm.v6i4.4597

Abstract

Gizi merupakan aspek yang penting untuk tumbuh kembang balita. Salah satu komponen gizi yang penting untuk balita adalah protein. Salah satu bahan makanan yang mengandung protein tinggi dan bagus untuk balita adalah ikan. Desa Ngaprah merupakan salah satu desa di Kabupaten Semarang yang berada di kawasan Rawa Pening yang kaya akan sumber daya alam berupa ikan. Roro Mendut merupakan salah satu UMKM yang berada di Desa Ngaprah Kecamatan Banyubiru, yang memanfaatkan ikan dari Rawa Pening untuk membuat produk jualannya. Rumusan masalah yang muncul adalah kurang memadainya peralatan produksi, terutama peralatan yang menunjang kualitas kesehatan dan gizi, serta peralatan yang menunjang kuantitas produk, produk mitra masih sederhana dan kurang variatif, masih kurangnya kepedulian masyarakat untuk menjaga kelestarian ikan di Rawa Pening. Metode yang digunakan untuk menyelesaikan masalah tersebut adalah dengan melakukan kegiatan pengabdian masyarakat yang meliputi sosialisasi, pelatihan peningkatan kualitas produk melalui diversifikasi frozen food untuk balita, penerapan teknologi, pendampingan dan evaluasi, serta keberlanjutan program. Hasil dari kegiatan pengabdian masyarakat ini adalah terdapat peningkatan pengetahuan tentang diversifikasi frozen food untuk balita dan konsep blue economy dikalangan industri pada seluruh peserta pelatihan. Terdapat peningkatan kuantitas dan kualitas produknya dalam aspek jenis, jumlah, serta higienitas produk pada UMKM Dapur Roro Mendut.
EFEKTIVITAS KRIM FORMULASI TEH HIJAU (Camellia sinensis) SEBAGAI AGEN ANTIBAKTERI TERHADAP PATOGEN KULIT Prayogo, Fitra Adi; Wijayanti, Heni; Rahmawati, Novita; Budiharjo, Anto; Wulandari, Dyah
Greensphere: Journal of Environmental Chemistry Vol 5, No 1 (2025): Volume 5 Nomor 1 Tahun 2025
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/gjec.2025.26815

Abstract

This research aims to determine the effect of green tea extract cream 6%, 8%, and 10% w/w against Staphylococcus epidermidis and Propionibacterium acne. The method begins with making green tea extract using the maceration method, and qualitative flavonoid, alkaloid, saponin, and tannin tests are carried out. Next, S. epidermidis and P. acnes were rejuvenated on NA media and cultured at 37°C for 24 hours. Both bacteria were then inoculated into different test tubes containing 0.9% NaCl. The suspension was then vortexed, and the turbidity level was equalized with McFarland's solution. Both bacteria were then cultured on NA media, and paper disks that had previously been soaked in clindamycin (control +) and 0%, 6%, 8%, and 10% green tea extract cream were placed on the agar media. The culture was then incubated for 24 hours at 37°C, and the inhibition zone was checked with a caliper. Results show that green tea extract has positive tests for flavonoids, alkaloids, saponins, and tannins. The optimum concentration of extract cream on antibacterial activity against S. epidermidis and P. acne is 10%. Inhibition zone comparison showed a significant difference between the diameter of the inhibition zone of S. epidermidis and P. acne, p = 0,00485 (p < 0,05), which means that green tea extract cream is better at fighting P. acne.
Fitoremediasi Logam Besi (Fe) dalam Lindi TPA Jatibarang Menggunakan Echinodorus palaefolius: Pengaruh Waktu Kontak terhadap Efisiensi Penyerapan Sonya Kirana Sari, Mellyaning Oktaviani; Prayogo, Fitra Adi
Greensphere: Journal of Environmental Chemistry Vol 4, No 2 (2024): Vol 4, No 2(2024)
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/gjec.2024.26909

Abstract

Penelitian ini mengevaluasi efektivitas tanaman Echinodorus palaefolius dalam sistem lahan basah buatan untuk remediasi besi (Fe) dari lindi yang dihasilkan oleh tempat pembuangan akhir (TPA). Eksperimen dilakukan dalam kondisi terkendali dengan empat waktu kontak (0, 7, 14, dan 21 hari) menggunakan tiga ulangan untuk setiap perlakuan. Hasil penelitian menunjukkan bahwa E. palaefolius berhasil menghilangkan 95,98% Fe dari lindi, dengan konsentrasi Fe dalam tanah berkurang sebanyak 29,49% pada hari ke-7 (p < 0,01). Uji ANOVA dan Duncan’s Multiple Range Test (DMRT) mengonfirmasi signifikansi statistik dari temuan ini. Dibandingkan dengan metode konvensional seperti presipitasi kimia, penggunaan E. palaefolius menawarkan biaya yang lebih rendah, efisiensi yang lebih tinggi, dan limbah sekunder yang minimal, menjadikannya solusi yang berkelanjutan untuk penanganan lindi. Penelitian ini menyimpulkan bahwa E. palaefoliusmerupakan biosorben yang efektif untuk remediasi Fe, dengan waktu kontak optimal pada hari ke-7, yang menghasilkan konsentrasi Fe akhir pada lindi yang memenuhi standar kualitas air untuk pembuangan ke Sungai Kreo sesuai Peraturan Daerah Jawa Tengah No. 5 Tahun 2012. Temuan ini menyoroti potensi E. palaefolius sebagai metode fitoremediasi yang ramah lingkungan dan dapat diterapkan dalam skala besar untuk pengelolaan limbah TPA.
Ekstraksi DNA Pseudomonas aeruginosa: Komparasi Metode NaOH, Perebusan, Iradiasi Microwave, dan Kit Komersial. Prayogo, Fitra Adi; Chakim, Irfanul
Praxis : Jurnal Sains, Teknologi, Masyarakat dan Jejaring Vol 7, No 2 : March 2025
Publisher : Soegijapranata Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24167/praxis.v7i2.13313

Abstract

This study aims to compare the effectiveness of four methods of DNA extraction from Pseudomonas aeruginosa bacteria: NaOH, boiling, microwave, and a commercial kit. The four methods were evaluated based on DNA quantity and quality, as well as time and cost efficiency. Results showed that the microwave method yielded the highest DNA concentration (92.23 ± 1.04 ng/µL) with optimal purity (A260/A280 ratio of about 1.82). The boiling method had the highest purity ratio (1.90 ± 0.05), but failed to produce PCR amplification despite its high concentration. The commercial kit produced the best quality DNA but with the lowest concentration (14.35 ± 0.74 ng/µL) and highest cost (IDR 50,000.00 per sample). In terms of efficiency, the microwave and boiling methods were the most cost (IDR 500.00 per sample) and time (less than 15 minutes) efficient. Based on the total analysis, the microwave method offers the best balance between quality, quantity, time, and cost, so it is recommended as an efficient alternative for research and diagnostic applications, especially in microbiological surveillance or epidemiological studies. However, for sensitive downstream applications such as genome sequencing, the use of commercial kits remains safer despite the high cost.
Characterization of lactic acid bacteria isolated from stinky tofu and suan-tsai using API 50 CHL Budiatmoko, Aurelia Wibowo; Wulandari, Dyah; Hwang, Chin-Fa; Budiharjo, Anto -; Prayogo, Fitra Adi
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.72963

Abstract

Stinky tofu and suan-tsai are traditional Taiwanese fermented foods produced through spontaneous fermentation involving lactic acid bacteria (LAB). This study aimed to explore the diversity of lactic acid bacteria present in suan-tsai and stinky tofu through the isolation, characterization, and identification of bacterial strains based on characteristics and the API 50 CHL Kit with 16S rRNA gene sequencing considered for future confirmation. Samples were diluted and cultured on MRS agar with the addition of CaCO₃. The 20 colonies forming clear zones were selected for morphological and biochemical characterization including microscopy, gram staining, motility, and catalase activity. Two isolates exhibiting clear zone formation, Gram-positive staining, non-motile behavior, cocci or rod-shaped morphology, and negative catalase activity were selected for identification using the API 50 CHL test kit. One isolate from stinky tofu (A11) was identified as Leuconostoc mesenteroides with 99.8% similarity and, one isolate from suan-tsai (C9) was identified as Lactobacillus casei with 99.4% similarity. Both strains were gram-positive, non-motile, and catalase-negative, indicating LAB characteristics. L. mesenteroides is a heterofermentative bacterium that produces lactic acid, CO₂, and ethanol contributing to creating an acidic condition and flavor development. L. casei is a homofermentative bacterium that support maintenance of low pH, inhibits pathogenic microorganisms, and supports food preservation. The probiotic potential of these strains was based on literature reports describing the antimicrobial and health promoting properties. These findings highlight representative LAB from Taiwan traditional fermented foods and its potential role in preservation, quality, and functionality.
Spirulina sebagai Agen Kardioprotektif Potensial terhadap Dislipidemia Akibat Minyak Goreng Bekas: Studi Eksperimental dengan Wawasan Mekanistik: Spirulina sebagai Agen Kardioprotektif Potensial terhadap Dislipidemia Akibat Minyak Goreng Bekas: Studi Eksperimental dengan Wawasan Mekanistik Royhanaty, Isy; Prayogo, Fitra Adi; Amelia, Poppy Fransisca; Elza, Stefani; Wijayanti, Eka Anita; Falasifah, Falasifah
Amerta Nutrition Vol. 10 No. 1SP (2026): AMERTA NUTRITION SUPPLEMENTARY EDITION
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v10i1SP.2026.68-73

Abstract

Background: Repeatedly heated cooking oil promotes dyslipidemia and oxidative stress, both major risk factors for cardiovascular disease. Spirulina platensis, a microalga rich in bioactive compounds, has been proposed as a protective agent against these effects. Objectives: This study evaluated the protective effects of Spirulina platensis on dyslipidemia and oxidative stress induced by repeatedly heated cooking oil in Wistar rats. Methods: Eighteen male Wistar rats (220–250 g) were divided into three groups (n=6): a negative control (standard diet), a positive control (standard diet plus heated cooking oil, 2 mL/rat/day), and a treatment group (heated oil plus spirulina, 330 mg/kg BW/day). After 28 days, total cholesterol, triglycerides, HDL-C, LDL-C, atherogenic index, MDA, GSH, and SOD were measured spectrophotometrically. Results: Compared with the positive control, spirulina lowered total cholesterol from 180.0±15.6 to 140.0±9.5 mg/dL (−22.2%, p-value<0.001), triglycerides from 150.0±18.7 to 100.0±10.3 mg/dL (−33.3%, p-value<0.001), and LDL-C from 120.0±16.8 to 80.0±8.7 mg/dL (−33.3%, p-value<0.001). HDL-C rose from 30.0±4.2 to 45.0±6.1 mg/dL (+50.0%, p-value<0.001), while the atherogenic index fell from 5.0±0.8 to 2.1±0.4 (−58.0%, p-value<0.001). Oxidative stress markers also improved: MDA decreased from 7.28±0.92 to 4.33±0.58 nmol/mL (−40.5%), GSH increased from 2.20±0.38 to 3.44±0.46 µmol/L (+56.4%), and SOD from 4.18±0.72 to 6.97±0.83 U/mL (+66.7%) (all p-value<0.001). Conclusions: Spirulina supplementation (330 mg/kg BW/day) significantly attenuated dyslipidemia and oxidative stress induced by repeatedly heated cooking oil, improving lipid parameters by 22–50% and antioxidant status by 40–67%. These findings suggest spirulina has cardioprotective potential against oxidized dietary fats.
HARNESSING THE POTENTIAL OF ASTAXANTHIN FROM Haematococcus pluvialis IN BIOMEDICAL SCIENCE: A NARRATIVE REVIEW Fitra Adi Prayogo; Mellyaning Oktavia Sonya Kirana Sari; Ni Nyoman Maryaningtyas Adinatha
Journal of Biomedical Sciences and Health Vol. 2 No. 1 (2025)
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jbsh.v2.i1.196

Abstract

Astaxanthin merupakan karotenoid bernilai tinggi dengan berbagai aplikasi, salah satunya dalam bidang ilmu biomedis. Haematococcus pluvialis dianggap sebagai sumber astaxanthin alami yang paling menjanjikan karena dapat mengakumulasi hingga 5% dari berat keringnya dalam kondisi yang penuh tekanan. Potensi aplikasi astaxanthin dalam biomedis sangat luas. Dari efek neuroprotektif hingga sifat antikanker dan manfaat kardiovaskularnya, astaxanthin telah menunjukkan janji dalam mengatasi beberapa tantangan kesehatan yang paling mendesak. Selain itu, sifat antiinflamasinya dan kemampuannya untuk memodulasi berbagai jalur pensinyalan semakin menggarisbawahi potensinya sebagai agen terapeutik. Tinjauan naratif ini memberikan sintesis komprehensif dari penelitian terkini tentang astaxanthin, merinci sifat biologisnya, tantangan budidaya, dan aplikasi terapeutik yang luas. Wawasan utama meliputi efek neuroprotektif, antiinflamasi, antikanker, dan kardioprotektifnya, yang didukung oleh struktur molekulnya yang unik dan sifat amfifiliknya. Dengan mengevaluasi secara kritis kemajuan terkini dalam teknik budidaya, ekstraksi, dan formulasi, tinjauan ini mengidentifikasi keterbatasan saat ini dan menguraikan berbagai kemungkinan untuk penelitian di masa mendatang. Secara keseluruhan, astaxanthin merupakan agen multifaset dengan potensi klinis yang signifikan, yang memerlukan penelitian lebih lanjut untuk mengoptimalkan bioavailabilitas dan kemanjuran terapeutiknya..   Kata kunci: Astaxanthin; Haematococcus pluvialis; Antioksidan; Aplikasi biomedis   ABSTRACT Astaxanthin is a high-value carotenoid with various applications, one of which is in biomedical science. Haematococcus pluvialis is considered the most promising source of natural astaxanthin as it can accumulate up to 5% of its dry weight under stressful conditions. The potential applications of astaxanthin in biomedicine are vast. From its neuroprotective effects to its anticancer properties and cardiovascular benefits, astaxanthin has shown promise in addressing some of the most pressing health challenges. Moreover, its anti-inflammatory properties and ability to modulate various signaling pathways further underscore its potential as a therapeutic agent. This narrative review provides a comprehensive synthesis of current research on astaxanthin, detailing its biological properties, cultivation challenges, and wide-ranging therapeutic applications. Key insights include its neuroprotective, anti-inflammatory, anti-cancer, and cardioprotective effects, underpinned by its unique molecular structure and amphiphilic nature. By critically evaluating recent advancements in cultivation, extraction, and formulation techniques, this review identifies current limitations and outlines potential avenues for future research. Overall, astaxanthin represents a multifaceted agent with significant clinical potential, warranting further studies to optimize its bioavailability and therapeutic efficacy. Keywords: Astaxanthin; Haematococcus pluvialis; Antioxidant; Biomedical applications
Co-Authors Adinatha, Ni Nyoman Maryaningtyas Agung Suprihadi AMELIA, POPPY FRANSISCA Anto Budiharjo Anto Budiharjo Benjarat Bunterngsook Budi Raharjo Budiatmoko, Aurelia Wibowo Budiharjo Anto Budiharjo, Anto - Dyah Wulandari Dyah Wulandari Edwin Setiawan Elza, Stefani Falasifah, Falasifah Heni Wijayanti, Heni Hermin Pancasakti Kusumaningrum Huda, Muhammad Badrul Hwang, Chin-Fa Irfanul chakim, Irfanul Iswandilevani, Atalia Nilam Mellyaning Oktavia Sonya Kirana Sari Michael Einstein Hermanto Ni Nyoman Maryaningtyas Adinatha Novita Rahmawati, Novita Novita Sari Pattanop Kanokratana Riani Catur Royhanaty, Isy Sonya Kirana Sari, Mellyaning Oktaviani Wijayanti, Eka Anita Yanuar Ahmad