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All Journal CHEMISTRY PROGRESS JOURNAL SCIENTIFIC OF MANDALIKA (JSM)
Yohanis Mandik
Universitas Cenderawasih
Humanities Chemistry Languange, Linguistic, Communication & Media Other

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KARAKTERISASI PRODUK GEN PENGKODE CHAPERONE BACILLUS SP. RP1 Kumaunang, Maureen; Mandik, Yohanis
CHEMISTRY PROGRESS Vol 3, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.1.2010.75

Abstract

Molecular chaperone is the group of protein that assist in the correct folding or assembly of other proteins invivo. The objective of this research was to characterize the product of gene encoding chaperone from Bacillussp. RP1 by using in-silico analysis. Analysis of 1053 bp from DNA fragment isolated by PCR showed that it has97% similarity with putative chaperone of Geobacillus kaustophilus (BA000043.1) and 86% similarity with DnaKoperon of B. stearothermophilus (X90709.1). Structure prediction of DnaJ Bacillus sp. RP1 showed that it hassimilar J-domain with that of DnaJ Escherichia coli but cys-rich domain is different.
ANALISIS IN-SILICO PROTEIN DnaJ Bacilus stearothermophilus Kumaunang, Maureen; Kamu, Vanda S.; Mandik, Yohanis I.
CHEMISTRY PROGRESS Vol 2, No 1 (2009)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.2.1.2009.63

Abstract

DnaJ is a chaperone which has function in facilitating folding, translocation, and degradation of protein.The aim of this research was to undertake in silico study of DnaJ protein from Bacillus stearothermophilus.Structure analysis of DnaJ B. stearothermophilus showed that it has J-domain which has two conservedmotifs, i.e. HPD and QKRA motifs. It also has cys-rich domain which has one conserved motif, i.e.CXXCXGXG. Structure prediction of DnaJ B. stearothermophilus showed that it has similar structure of Jdomainand cys-rich domain with that of DnaJ Escherichia coli.
Pengaruh Kadar Air Terhadap Tingkat Keasaman Tanah Kampung Burmeso Secara Elektrometri Sineri, Carli N. M.; Krimadi, Lodwyk N.; Salim, Ilham; Mandik, Yohanis I.; Rusmanta, Yohanes B. J.
Journal Scientific of Mandalika (JSM) e-ISSN 2745-5955 | p-ISSN 2809-0543 Vol. 6 No. 6 (2025)
Publisher : Institut Penelitian dan Pengembangan Mandalika Indonesia (IP2MI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/10.36312/vol6iss6pp1533-1540

Abstract

A study has been conducted on the analysis of the effect of moisture content on the acidity level (pH H2O and pH KCl) of the soil of Burmeso Village using the electrometry method. Samples were taken from a depth of 0-10 cm with a sample soil ring. Next, the soil samples were dried at 105°C for 3 hours, sifted using a 200 mesh sieve. The analysis was carried out on the physical properties (moisture content) and its correlation with the chemical properties (Soil Acidity). The results of this study showed that the soil moisture content of three soil samples was 9.03 (TL), 9.065 (TH), and 9.56 (TB) and was classified as high enough moisture content so that it had a direct effect on the pH of farmland, forest soil and ex-field soil was classified as sour (low) to slightly sour (moderate), the high and low pH greatly affected the nutrient content in the soil so that it produced. The results of soil acidity analysis also indicate that the actual acidity pH value (H2O pH) is greater when compared to the potential pH (pH KCl) so that the ΔpH value tends to be negative including -0.705 (TL), -0.84 (TH), and -1.035 (TB), this negative value shows that the soil of Burmeso Village is a negative net load soil occupied by exchangeable bases (KTK).
Co-Authors Lodwyk Nomenzen Krimadi Maureen Kumaunang Rusmanta, Yohanes B. J. Salim, Ilham Sineri, Carli N. M. Vanda S. Kamu