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All Journal Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Journal of ICT Research and Applications JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Biodiversitas Journal of Biological Diversity
KIKIN HAMZAH MUTAQIN
Department Of Plant Protection, Faculty Of Agriculture, Bogor Agricultural University, Jalan Meranti, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology Veterinary

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Geminiviruses Associated with the Weed Species Ageratum conyzoides, Centipeda minima, Porophyllum ruderale, and Spilanthes iabadicensis from Java, Indonesia RIKA MELIANSYAH; SR HENDRASTUTI HIDAYAT; KIKIN HAMZAH MUTAQIN
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.939 KB) | DOI: 10.5454/mi.5.3.4

Abstract

Geminivirus has a wide host range including cultivated plants and weeds. Infected weeds may play an important role in disease epidemic. Unfortunately, little is known about weeds species that may serve as alternative host for Geminivirus. This research was conducted to identify Geminivirus on weeds around chili pepper field to study their potential role as virus reservoir. Field surveys were conducted to chilli pepper growing area inWest and Central Java Provinces, and The Special Province of Yogyakarta during 2009 to collect symptomatic weed plants. Geminivirus infection was detected using PCR technique from 9 weed samples, i.e. 5 samples Ageratum conyzoides from Bogor (AgrBgr), Sukabumi (AgrSkm), Magelang (AgrMgl), Sleman (AgrJgy), and Garut (AgrGrt); Centipeda minima from Magelang (CtpMgl); A. boehmerioides from Sleman (AcpJgy); Porophyllum ruderale from Bogor (PrlBgr); Spilanthes iabadicensis from Magelang (SplMgl). Further genetic analysis showed that those geminiviruses can be differentiated into 2 clusters, showing the possible genetic differences among them. They neither have a close relationship with other geminiviruses published earlier in the GenBank, indicating weed infecting collected Geminivirus in this study is possibly a distinct Geminivirus.
Identification of mealybugs on Piper nigrum as vector of Piper yellow mottle virus (Badnavirus: Caulimoviridae) Miftakhurohmah Miftakhurohmah; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; Bonny Poernomo Wahyu Soekarno; Dono Wahyuno
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222144-153

Abstract

Piper yellow mottle virus (PYMoV) is the dominant virus that causes mottle disease in black pepper (Piper nigrum). Two species of mealybugs, Ferrisia virgata and Planococcus minor have been reported as vectors of PYMoV. A different species of mealybug that has never been reported before was found in black pepper. Molecular approaches including total DNA isolation of mealybug collected from the field, mealybugs identification by DNA barcode, detection of PYMoV in single mealybugs, were conducted as an approach to identify the potential of mealybugs as PYMoV vector in the field. Mealybugs were collected from black pepper plants in Cimanggu (Bogor, West Java) and Sukamulya (Sukabumi, West Java). Characters of adult females were observed for morphological identification. Molecular-based identification of the mealybugs and PYMoV involved the following procedures: total DNA isolation, DNA amplification, nucleotide sequencing and sequence analysis. Three species of mealybugs, P. minor, F. virgata and Paracoccus marginatus were confirmed by morphological and molecular identification. This is the first report for the occurrence of P. marginatus in black pepper plants. PYMoV was successfully detected from field samples of F. virgata, P. minor and P. marginatus. This finding indicates the potential of insect vectors for disease spread and distribution.
Characteristics of Virus Symptoms in Chili Plants (Capsicum frutescens) Based on RGB Image Analysis Asmar Hasan; Widodo Widodo; Kikin Hamzah Mutaqin; Muhammad Taufik; Sri Hendrastuti Hidayat
AGRIVITA, Journal of Agricultural Science Vol 44, No 3 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.3731

Abstract

Virus infection in chili plants may cause various symptoms. The complexity of the symptoms and human vision ability often become limiting factors during disease investigations. Digital image analysis is expected to become a method to assist in comprehensively describing the symptoms of plant viruses. A disease survey was conducted on cayenne pepper fields in Southeast Sulawesi Province to observe symptoms of virus infection virtually and to record the symptomatic plant using an RGB camera. The split-channel method is used to process images, followed by multidimensional scaling statistical analysis. Later on, viruses associated with plants were detected serologically. Single or mixed infection of Tobacco mosaic virus, Cucumber mosaic virus, Chili veinal mottle virus, and Pepper mottle virus was confirmed by plant leaves showing yellow-mosaic and mottle symptoms. The digital image analysis method could show variations in the characteristics of symptoms based on digital numbers in that cannot be recognized based on the observation of visual symptoms. A new approach to study the interactions between plant infecting viruses and their effects based on image analysis has also been developed during this research. This method needs to be further validated through testing under controlled conditions, such as inoculating plants with a predetermined type of virus.
The Potential of a Low-Cost Thermal Camera for Early Detection of Temperature Changes in Virus-Infected Chili Plants Asmar Hasan; Widodo Widodo; Kikin Hamzah Mutaqin; Muhammad Taufik; Sri Hendrastuti Hidayat
Journal of ICT Research and Applications Vol. 17 No. 1 (2023)
Publisher : DRPM - ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/itbj.ict.res.appl.2023.17.1.2

Abstract

One effect of viral infection on plant physiology is increased stomata closure so that the transpiration rate is low, which in turn causes an increase in leaf temperature. Changes in plant leaf temperature can be measured by thermography using high-resolution thermal cameras. The results can be used as an indicator of virus infection, even before the appearance of visible symptoms. However, the higher the sensor resolution of the thermal camera, the more expensive it is, which is an obstacle in developing the method more widely. This article describes the potential of thermography in detecting Tobacco mosaic virus infection in chili-pepper plants using a low-cost camera. A FLIR C2 camera was used to record images of plants in two treatment groups, non-inoculated (V0) and virus-inoculated plants (V1). Significantly, V1 had a lower temperature at 8 and 12 days after inoculation (dai) than those of V0, but their temperature was higher than V0 before symptoms were visible, i.e., at 17 dai. Thermography using low-cost thermal cameras has potency to detect early viral infection at 8 dai with accuracy levels (AUC) of 80.0% and 86.5% based on k-Nearest Neighbors and Naïve Bayes classifiers, respectively.
Laporan Pertama Fitoplasma Berasosiasi dengan Penyakit Proliferasi Stroberidan Sapu Jalantir di Jawa Barat, Indonesia: First Report of Phytoplasmas Associated with Strawberry Proliferationand Tall Fleabane Witches’ broom Diseases in West Java, Indonesia Noor Sholihin; Kikin Hamzah Mutaqin; Giyanto
Jurnal Fitopatologi Indonesia Vol. 22 No. 1 (2026): Maret 2026: IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.22.1.1-6

Abstract

Fitoplasma adalah kelompok bakteri dari kelas Mollicute yang telah banyak dilaporkan berasosiasi dengan penyakit pada ratusan spesies tanaman di berbagai negara. Di Indonesia, fitoplasma baru diketahui berasosiasi dengan tanaman-tanaman inang lebih dari delapan famili berbeda. Penelitian ini bertujuan membuktikan asosiasi fitoplasma pada tanaman stroberi bergejala filodi dan suatu tanaman liar bergejala daun kecil di Jawa Barat dengan teknik nested-polymerase chain reaction (nested-PCR) menggunakan dua pasangan primer yang dirancang dari wilayah gen 16S-23S rRNA fitoplasma. Tumbuhan liar juga diidentifikasi secara morfologi dan molekuler berdasarkan wilayah ITS gen ribosomal RNA. Gejala penyakit pada stroberi (Fragaria ananassa) meliputi proliferasi tunas, filodi dan buah kerdil, sedangkan pada tumbuhan liar berupa sapu. Tumbuhan liar yang dikenal masyarakat sebagai jalantir berhasil diidentifikasi sebagai Conyza sumatrensis berdasarkan morfologi dan analisis nukleotida amplikon hasil PCR menggunakan pasangan primer universal ITS1/ITS4. Amplifikasi PCR awal dengan pasangan primer P1/P7 untuk fitoplasma dari stroberi dan jalantir menghasilkan pita DNA positif berukuran ±1800 pb dengan ketebalannya yang inkonsisten. Amplifikasi lanjut dengan nested-PCR dengan pasangan primer R16F2n/R16R2 menghasilkan pita DNA berukuran ±1250 pb yang lebih tebal. Hasil nested-PCR mengonfirmasi bahwa fitoplasma berasosiasi dengan dua tanaman tersebut. Penelitian ini merupakan laporan pertama fitoplasma berasosiasi dengan tanaman stroberi (Rosaceae) dan jalantir (Asteraceae).
MOLECULAR CHARACTERIZATION OF BEGOMOVIRUS INFECTING YARD LONG BEAN (Vigna unguiculata SUBSP. sesquipedalis L.) IN JAVA, INDONESIA Sari Nurulita; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; John Thomas
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.401

Abstract

Begomovirus has been identified as one of the causal agents associated with yellow mosaic disease in yardlong bean (Vigna unguiculata subsp. sesquipedalis (L.)) in Java. Previous studies reported that Begomovirus has infected several Leguminosae species in South Asia, and several Begomoviruses have also been reported to infect important crops in Indonesia based on nucleotide sequence characterization. This study was conducted to identify and characterize Begomovirus isolated from yardlong bean samples in Java based on specific genomic features of the Common Region. Samples were collected from yardlong bean fields in Central Java (Tegal, Magelang, and Klaten), Yogyakarta (Sleman), and West Java (Bogor and Subang); viral detection was performed using I-ELISA, PCR, and sequencing; and molecular characterization was conducted using BioEdit v.7.0.5 and MEGA 6.06 software. Yellow mosaic disease was observed in nearly all surveyed fields, and infection of Potyvirus and Begomovirus was detected either as single or mixed infection. PCR results confirmed Begomovirus infection in samples from Tegal, Klaten, Magelang, Subang, and Bogor. Sequence analysis revealed that the virus infecting yardlong bean was Mungbean Yellow Mosaic India Virus (MYMIV) and shared close genetic similarity with isolates from Bangladesh, India, Pakistan, and Nepal. Further analysis revealed conserved regions surrounding the Common Region, including the “TATA box” sequence, hairpin-loop structure, repetitive sequences, and the conserved nonanucleotide TAATATTAC. This study provides the first report of MYMIV infection in Indonesia.
The potential of endophytic bacteria from the root of Eucalyptus pellita as a biocontrol agent against Ralstonia solanacearum Yuliana susanti; GIYANTO GIYANTO; MEITY SURADJI SINAGA; KIKIN HAMZAH MUTAQIN; BUDI TJAHJONO2
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220654

Abstract

Abstract. Susanti Y, Giyanto, Sinaga MS, Mutaqin KH, Tjahjono B. 2021. The potential of endophytic bacteria from the root of Eucalyptus pellita as a biocontrol agent against Ralstonia solanacearum. Biodiversitas 22: 3454-3462. Bacterial wilt disease (BWD) caused by Ralstonia solanacearum is one of the most damaging eucalyptus (Eucalyptus pellita F. Muell.) diseases. However, endophytic bacteria can be utilized to control it. This study aims to obtain and screen endophytic bacteria isolated from the root of eucalyptus as a biocontrol agent against BWD. A pathogenic R. solanacearum was isolated for artificial inoculation; subsequently, isolation, characterization and screening of the potential endophytic bacteria were conducted. Isolates of R. solanacearum demonstrated the pathogenicity for BWD in eucalyptus, with the highest virulence found in Rs 18. In total, 24 isolates of endophytic bacteria were obtained and screened for their biocontrol potential. The endophytic bacteria conveyed the capacity of antibiosis, indole acetic acid (IAA) hormone, nitrogen fixation, phosphate dissolution, protease enzyme and AHL-lactonase, which are the main indicators of bioagent characteristics in endophytic bacteria, as well as being plant growth stimulators. Four isolates were selected with the highest according to the analytic hierarchy process (AHP), identified on the 16S rRNA gene. Most isolates belonged to gram-negative bacteria. The four isolates, namely B33/77 (Serratia nematodiphila), B32/77 (Paenibacillus polymyxa), B13/497 (Brevundimonas olei) and B17/111 (Bacillus megaterium), indicated their potential for biocontrol as plant growth promoters. This study is the first report on the isolation of endophytic bacterial from eucalyptus roots with regard to its benefit as a plant growth promoter. It provides basic information about the characteristics of the endophytic bacteria, which showed potential as a biocontrol agent of R. solanacearum on eucalyptus.
Co-Authors , Giyanto Asmar Hasan Bonny Poernomo Wahyu Soekarno BUDI TJAHJONO2 Dono Wahyuno GIYANTO GIYANTO John Thomas MEITY SURADJI SINAGA Miftakhurohmah Miftakhurohmah Muhammad Taufik Muhammad Taufik Noor Sholihin Rika Meliansyah Sari Nurulita SR HENDRASTUTI HIDAYAT SRI HENDRASTUTI HIDAYAT Widodo Widodo Widodo Widodo Yuliana susanti