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All Journal Jurnal Fitopatologi Indonesia Agrikultura Biotika: Jurnal Ilmiah Biologi Bionatura Microbiology Indonesia Cropsaver : Journal of Plant Protection Jurnal Perlindungan Tanaman Indonesia Jurnal Agrotek Tropika Soil REns Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Rika Meliansyah
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran Jalan Raya Bandung-Sumedang KM 21. Jatinangor, Indonesia 45363
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Paramita, Hedi; Puspasari, Lindung Tri; Maharani, Rani; Supratman, Unang; Hidayat, Yusup; Meliansyah, Rika; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Potensi Cuka Kayu Pinus dalam Pengendalian Penyakit Antraknosa pada Cabai Merah Sri Hartati; Rika Meliansyah; Lindung Tri Puspasari
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.374 KB) | DOI: 10.14692/jfi.9.6.173

Abstract

Anthracnose disease caused by Colletotrichum capsici is a major disease on red chili which causes great economic losses. Wood vinegar, as a result of condensation of smoke, can be used as bactericide and fungicide. The use of pine wood vinegar is prospective for controlling anthracnose disease. The objective of this research was to study the potency of pine wood vinegar in controlling anthracnose on red chili. In vitro test was carried out to measure the inhibition of pine wood vinegar against the growth of mycelium mass and the germination of the conidium of C. capsici. The treatments were the concentration of the pine wood vinegar, i.e. 0, 1.5, 2.5, 3.5, 4.5, and 5 %, and a fungicide (2% of antracol) as comparison. The results showed that the lower the concentration of the pine wood vinegar, the lower the inhibition of the mycelium growth. The highest inhibition of the mycelium growth (76.1%) was caused by 5% of the pine wood vinegar, followed by 41.04% inhibition caused by 4.5% concentration. There was no germination of the conidium on all treatments.
Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Hedi Paramita; Lindung Tri Puspasari; Yusup Hidayat; Rika Meliansyah; Danar Dono; Rani Maharani; Unang Supratman
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.565 KB) | DOI: 10.24198/cs.v1i1.16995

Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Bioactivity Fraction of Methanolic Seed Extract of Barringtonia asiatica L. (Kurz.) (Lecythidaceae) Against Spodoptera litura F. (Lepidoptera: Noctuidae) Teddy Budiyansyah; Danar Dono; Rika Meliansyah; Unang Supratman
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.142 KB) | DOI: 10.24198/cs.v1i2.19755

Abstract

Water seed extract of Barringtonia asiatica was used as fish poison by traditional community. This study aims to determine the bioactivity of methanolic seed extract fraction of B. asiatica against S. litura. The extract tested was spread evenly on the leaf surface by using microsyringe. The experiment consisted of 12 treatments and 3 replications. Treatments were concentration of 0.3% and 0.1% of fraction 16, 17, 18, 19, 20 of methanolic seed extract of B. asiatica, and concentration of 0.264% methanolic seed extract of B. asiatica; and control. The results showed that the fraction 19 (20% EtOAc and 80% MeOH eluent) of methanol extract of B. asiatica seed has the highest bioactivity against the larvae of S. litura. The fraction 19 of methanolic seed extract of B. asiatica had LC50 value of 0.182% at 12 days after treatment. In addition, the 19 fraction of methanolic seed extract of B. asiatica decreased food intake, weight and lengthened development time of larvae.Keywords : Barringtonia asiatica, bioactivity, fraction, Spodoptera litura.
Effect of Neem Oil Formulation on the Population of Soil Fungi and Disease Intencity of Cercospora Leaf Spot (Cercospora capsici) on Chilli Plants (Capsicum annuum) Sri Hartati; Danar Dono; Rika Meliansyah; Mukhamad Agung Yusuf
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.176 KB) | DOI: 10.24198/cs.v1i2.19753

Abstract

Soil fungi and cercospora leaf spot are soil inhabitants and plant disease, respectively, that usually occur on chilli grown area. Formulation of neem oil was reported to have a role as an environmentally friendly botanical pesticide. This research was objected to studying the effect of neem oil formulation on the population of soil fungi and its suppression on cercospora leaf spot intensity of chilli crop. The field experiment was arranged in the randomized complete block design. The treatments were the concentration of neem oil formulation, i.e. 0%, 1%, 1.25%, 1.50%, 1.75%, and 2.0%. In vitro experiment on the suppression of neem oil formulation on the predominant soil, fungi were arranged in the completely randomized design. The results showed that neem oil formulation did not affect the soil fungi population and cercospora leaf spot intensity. However,2.0% neem oil had the lowest soil population. Neem oil formulation at 1.75% caused the highest suppression on TMa01 isolate (49.81%), and 1.25%, 1.50%, 1.75% and 2.0% of neem oil formulation caused more than 50% suppression on TMf01 isolate in vitro. Neem oil formulation at 2.0% caused the highest suppression on cercospora leaf spot intensity (46.34%).Keywords: Azadirachta indica, botanical pesticide, neem oil
Bioaktivitas Formulasi Minyak Biji Azadirachta indica (A. Juss) terhadap Spodoptera litura F. Raden Arif Malik Ramadhan; Lindung Tri Puspasari; Rika Meliansyah; Rani Maharani; Yusup Hidayat; Danar Dono
Agrikultura Vol 27, No 1 (2016): April, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.974 KB) | DOI: 10.24198/agrikultura.v27i1.8470

Abstract

ABSTRACTBioactivity Formulation of Seed Neem Oil Azadirachta indica (A.Juss) against Spodoptera litura (F)The purpose of this research was to know the influence of neem seed extract formulation (Azadin 50 EC) on the mortality, larvae development, larvae weight, and food consumption of Armyworm (Spodoptera litura). This research used randomized complete design with 6 treatments and 5 replications, i.e. control, formula at concentration of 0.2%, 0.4%; 0.8%; 1.6%; and 3.2%. Correlation of concentration neem seed oil formulation and mortality of test insect was analysed using probit analysis, weight of test larvae presented in mean and standard deviation, and development time and food consumption of test larvae analysed with analysis of varians. Formula Azadin 50 EC had LC50 value of 0.659% (0.550-0.781%) at 12 days after treatment. The mortality increased significantly in pupae stage that caused LC50 value become 0.152%. The formula prolonged development time, decrease the weight of test insect and decrease food consumption by the larvae.Keywords: Lethal concentration, Mortality, Growth derangement, ExtractABSTRAKPenelitian ini bertujuan untuk mempelajari perlakuan formulasi minyak biji mimba Azadin 50 EC terhadap mortalitas, perkembangan larva, bobot larva dan konsumsi pakan ulat grayak (Spodoptera litura F.). Metode Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dengan 5 kali ulangan. Perlakuan tersebut yaitu : Kontrol, konsentrasi formula minyak mimba Azadin 50 EC 0,2%; 0,4%; 0,8%; 1,6% dan 3,2%. Hubungan mortalitas dengan konsentrasi formula dianalisis menggunakan analisis probit, sedangkan data bobot larva, konsumsi pakan dan waktu perkembangan larva dianalisis dengan sidik ragam. Hasil penelitian menunjukkan bahwa formula minyak biji A. Indica memiliki nilai LC50 sebesar 0,659% (0,550-0,781%) terhadap larva instar 2 hingga instar 4. Pada fase pupa kematian kembali meningkat tajam sehingga nilai LC50 menjadi 0,152%. Perlakuan formula tersebut mengakibatkan perpanjangan waktu perkembangan, menurunkan bobot, dan menurunkan konsumsi pakan larva uji.Kata Kunci: Konsentrasi letal, Mortalitas, Gangguan perkembangan, Ekstrak
FLUKTUASI POPULASI HAMA WERENG BATANG COKLAT (Nilaparvata lugens Stal.) DAN SPESIES MUSUH ALAMINYA DI DATARAN MENENGAH KECAMATAN LELES KABUPATEN GARUT Martua Suhunan Sianipar; Rika Meliansyah; Alghif Aruni Nur Rukman
BIOTIKA Jurnal Ilmiah Biologi Vol 14, No 1 (2016): BIOTIKA JUNI 2016
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v14i1.14412

Abstract

Wereng Batang Cokat (Nilaparvata lugens Stal.) merupakan hama utama tanaman padi.Penelitian ini bertujuan mempelajari ǃuktuasi populasi WBC dan spesies musuh alaminya pada tanaman padi di dataran menengah. Penelitian ini dilakukan dengan metode survei. Survei dilakukan pada tiga lahan percobaan yang bertempat di Kecamatan Leles Kabupaten Garut dan di rumah kaca Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran. Metode pengambilan sampel dilakukan secara diagonal menggunakan perangkap kuning serta manual atau visual counting. Hasil penelitian menunjukkan suhu, kelembaban dan curah hujan tidak memberikan pengaruh yang signiƶkan terhadap ǃuktuasi populasi WBC. Hal ini ditunjukan dengan hasil analisis regresi masing-masing pada suhu (Y= -2,559 + 66,470 x ; R2=0,054 ; P = 0,519), kelembaban (Y= -1,378 + 105,347x ; R2=0,332 ; P = 0,081) dan curah hujan (Y= 0,175 + 4,803x ; R2=0,248 ; P = 0,143). Indeks keragaman musuh alami dari rendah sampai sedang.
PENGENDALIAN BIOLOGI NEMATODA Meloidogyne spp. DENGAN JAMUR Paecilomyces fumosoroseus DAN BAKTERI Pasteuria penetrans SERTA PENGARUHNYA TERHADAP TANAMAN BUNCIS (Phaseolus vulgaris L.) Toto Sunarto -; Luciana Djaja -; Rika Meliansyah -
Bionatura Vol 11, No 1 (2009): Bionatura Maret 2009
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.229 KB)

Abstract

Produksi buncis di Indonesia masih rendah dibandingkan dengan negara-negara lain. Hal ini diakibatkan oleh serangan nematoda bengkak akar (Meloidogyne spp.). Usaha pengendalian Meloidogyne spp. dapat dilakukan dengan beberapa cara, salah satunya dengan pemanfaatan musuh alami Meloidogyne spp. yaitu Paecilomyces fumosoroseus dan Pasteuria penetrans. Penelitian ini bertujuan untuk memperoleh efektifitas P. fumosoroseus, dan P. penetrans, atau campuran keduanya terhadap indeks gall akar, jumlah telur, berat segar bagian atas tanaman, jumlah larva II Meloidogyne spp. dalam 100 ml tanah, dan hasil tanaman buncis. Penelitian menggunakan metode percobaan dengan Rancangan Acak Kelompok, terdiri atas 8 perlakuan dan 4 ulangan. Perlakuan tersebut adalah tanaman buncis diinokulasi dengan: 1). Meloidogyne spp. + P. fumosoroseus, 2). Meloidogyne spp. + Pasteuria penetrans, 3). Meloidogyne spp. + P. fumosoroseus + P. penetrans, 4). Hanya Meloidogyne spp., 5). Hanya P. fumosoroseus, 6). Hanya P. penetrans, 7). P. fumosoroseus + P. penetrans. 8). Kontrol tanpa perlakuan. Data hasil percobaan dianalisis dengan menggunakan analisis ragam. Hasil percobaan menunjukkan, bahwa perlakuan yang mengandung Meloidogyne spp. dan P. fumosoroseus, atau Meloidogyne spp. dan P. penetrans, atau Meloidogyne spp. ditambah P. fumosoroseus dan P. penetrans mampu menurunkan indeks gall akar, jumlah telur, mampu meningkatkan berat segar bagian atas tanaman, menurunkan jumlah larva II Meloidogyne spp. dalam 100 ml tanah, dan meningkatkan hasil tanaman buncis. P. fumosoroseus dan P. penetrans yang diaplikasikan bersama mampu menurunkan jumlah telur, dan jumlah larva II Meloidogyne spp. dalam 100 ml tanah, dan hasil buncis lebih tinggi dibandingkan jika diaplikasikan secara sendiri-sendiri.Kata kunci : Pengendalian secara biologi, Meloidogyne spp., Paecilomyces fumosoroseus, Pasteuria penetrans, buncis
Potency of Yeasts to Promote Induced Resistance on Chili Plant (Capsicum annuum L.) against Yellow Leaf Curl Disease Sri Hartati; Rika Meliansyah; Luciana Djaya; Ceppy Nasahi; Ramadhan Kamal Putra
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.52089

Abstract

Yellow leaf curl is a major disease on chili plants. The use of antagonistic yeasts as the control agents is a good alternative in  an environmentally friendly control method. This study was objected to evaluate the potencies of Rhodotorula minuta and Candida tropicalis to promote induced resistance on chili plants against yellow leaf curl disease. The experiment was arranged in the randomized complete block design with 9 treatments and 3 replications. The treatments were the application of the yeasts at three different times of virus inoculation, as follows: A. R. minuta, virus inoculation at 3 days after transplanting (dat), B. R. minuta, virus inoculation at 7 dat, C. R. minuta, virus inoculation at 10 dat, D. C. tropicalis, virus inoculation at 3 dat, E. C. tropicalis, virus inoculation at 7 dat, F. C. tropicalis, virus inoculation at 10 dat, G. control, virus inoculation at 3 dat, H. control, virus inoculation at 7 dat, and I. control, virus inoculation at 10 dat. No treatment was applied to the control.  The yeasts were applied by soaking the chili seeds, and pouring the suspension into the growth media at transplanting. The variables observed were incubation period, disease severity, and disease incidence. Peroxidase activity, phenylalanine ammonia lyase activity, and salicylic acid accumulation were also analyzed. The results showed that the application of R. minuta or C. tropicalis was able to extend the disease incubation period, but did not reduce the yellow leaf curl disease incidence and severity. The treatment of R. minuta, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6870.93 units (0.5 minute) and from 577.367 units to 1131.300 units (2.5 minutes), PAL activity from 16.059 to 17.911 A290/mg, and accumulation of salicylic acid from 2.785 to 6.263 ppm. Application of C. tropicalis, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6033.067 units (0.5 minute) and from 577.367 units to 950.967 units (2.5 minutes), and accumulation of salicylic acid from 2.785 to 6.982 ppm.
Geminiviruses Associated with the Weed Species Ageratum conyzoides, Centipeda minima, Porophyllum ruderale, and Spilanthes iabadicensis from Java, Indonesia RIKA MELIANSYAH; SR HENDRASTUTI HIDAYAT; KIKIN HAMZAH MUTAQIN
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.939 KB) | DOI: 10.5454/mi.5.3.4

Abstract

Geminivirus has a wide host range including cultivated plants and weeds. Infected weeds may play an important role in disease epidemic. Unfortunately, little is known about weeds species that may serve as alternative host for Geminivirus. This research was conducted to identify Geminivirus on weeds around chili pepper field to study their potential role as virus reservoir. Field surveys were conducted to chilli pepper growing area inWest and Central Java Provinces, and The Special Province of Yogyakarta during 2009 to collect symptomatic weed plants. Geminivirus infection was detected using PCR technique from 9 weed samples, i.e. 5 samples Ageratum conyzoides from Bogor (AgrBgr), Sukabumi (AgrSkm), Magelang (AgrMgl), Sleman (AgrJgy), and Garut (AgrGrt); Centipeda minima from Magelang (CtpMgl); A. boehmerioides from Sleman (AcpJgy); Porophyllum ruderale from Bogor (PrlBgr); Spilanthes iabadicensis from Magelang (SplMgl). Further genetic analysis showed that those geminiviruses can be differentiated into 2 clusters, showing the possible genetic differences among them. They neither have a close relationship with other geminiviruses published earlier in the GenBank, indicating weed infecting collected Geminivirus in this study is possibly a distinct Geminivirus.
Co-Authors Agus Susanto Al Farisy, Muhammad Restu Alghif Aruni Nur Rukman Anne Nuraini Anni Yuniarti Aoliya, Salwa Rohmatul Ceppy Nasahi Danar Dono Dikdik Kurnia Endah Yulia Erni Suminar Fitri Widiantini Hedi Paramita Hersanti - Ichsan Nurul Bari Ida Ayu Putu Sri Widnyani KIKIN HAMZAH MUTAQIN Kusumadewi, Vira Lindung Tri Puspasari Luciana Djaja - Luciana Djaya Martua Suhunan Sianipar Mukhamad Agung Yusuf Narendra, Nathanael Dimas Noor Istifadah Nurochman, Irvan Sopyan Paramita, Hedi R. Arif Malik Ramadhan Rahmat Budiarto Ramadhan Kamal Putra Rani Maharani Rani Maharani Reynaldi Reynaldi Rizky Ramdhani Santika Sari Setiani, Cahya Siti Julaeha, Siti SR HENDRASTUTI HIDAYAT Sri Hartati Sri Hartati Sri Hartati Sudarjat Sudarjat Syariful Mubarok Tarkus Suganda Teddy Budiyansyah Tiara Febricyanti Putri Senjaya Toto Sunarto Toto Sunarto - Tri Mayanti Unang Supratman Vira Kusuma Dewi Warid Ali Qosim Wawan Kurniawan Yulianto, Fiky Yusup Hidayat Yusup Hidayat