Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

0.817

P-Index

This Author published in this journals

All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA PENDIPA Journal of Science Education Menara Perkebunan

Bonny Poernomo Wahyu Soekarno

Departemen Proteksi Tanaman, Institut Pertanian Bogor, Jl Kamper, Kampus Darmaga, Bogor 16680

Author-ID : 2451164

Agriculture, Biological Sciences & Forestry Chemistry Education Mathematics Medicine & Pharmacology

Published : 16 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Isolation and Capability of Dark Septate Endophyte Against Mancozeb Fungicide Jean Nihana Manalu; Bonny Poernomo Wahyu Soekarno; Efi Toding Tondok; Surono Surono
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Mancozeb is a group member of dithiocarbamat fungicides that most widely applied in the field. The use of mancozeb causes residues that have negative effects on agricultural production system and on agricultural environment. This research aimed to obtain dark septate endophyte (DSE) fungal isolates that are tolerant to mancozeb fungicide. The study was consisted of three steps, i.e., the isolation of DSE fungi from chili roots, the effect of DSE fungal isolates on chili seeds, and the growth ability of DSE isolates on medium supplemented with mancozeb. Thirteen DSE fungal isolates were isolated from chilli pepper roots and grown in the field assumed to be contaminated by pesticides. The isolates of DSE fungi caused the growth of chili seeds were normal and abnormal. Seven DSE isolates were able to grow well at PDA medium suplemented with mancozeb at concentrations of 100, 200, 400, and 800 ppm with different levels of relative growth inhibition. Mancozeb on PDA medium influenced the mycelial growth and colony color of DSE fungal isolates. The biomass of DSE fungal isolates were reduced when they were grown at malt extract broth (MEB) medium suplemented various levels of mancozeb. Keywords: dithiocarbamat, relative growth inhibition, tolerant

Potensi Ekstrak Kangkung sebagai Biofungisida untuk Mengendalikan Penyakit Busuk Buah Fusarium pada Tomat Bonny Poernomo Wahyu Soekarno; Surono .; Eva Marhaenis
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

One of the important pathogens on tomato is Fusarium sp. causing wilt and fruit rot. This study aims to investigate the potency of water spinach (Ipomea aquatica) as a biofungicide for inhibiting growth and development of tomato fruit rot caused by Fusarium sp. This study showed inhibiting ability of I. aquatica stem extract to Fusarium sp. growth ranges from 3.40% to 8.67%, while inhibiting ability of leaves extract can reach 3.40% to 45.55%. Resistance induction test showed that in vitro treatment of I. aquatica leaves extract 20% can lengthen incubation time of Fusarium fruit rot compared to positive and negative control. Leaves extract of I. aquatica 20% is potential as biofungicide.Key words: biofungicide, Fusarium sp., Ipomea aquatica

Minyak Nilam sebagai Biofungisida untuk Pengendalian Penyakit Antraknosa Cabai Desni Roha Miriam Sakerebau; Bonny Poernomo Wahyu Soekarno Poernomo Wahyu Soekarno
Jurnal Fitopatologi Indonesia Vol 9 No 3 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Anthracnose caused by C. capsici is the most important factor causing low yield on chili in Indonesia. A research was conducted to examine to examine the potential of patchouli oil in suppressing anthracnose disease development on chili. In vitro test was conducted by growing C. capsici on potato dextrose agar (PDA) medium that has been added with different concentration of patchouli oil, i.e. 10, 5, 2.5, 1.25 and 0.625%. Negative controls treatment (Kn) was conducted by growing C. capsici on PDA without patchouli oil, whereas positive controls (Kp) was conducted by growing C. capsici on PDA containing mancozeb fungicide. Patchouli oil -A significantly suppressed the growth of C. capsici compared to patchouli oil-B on 10 days after harvest, i.e. 91.15% suppression for all concentration level except for 0.625% which caused 85.92% suppression. Further examination using patchouli oil-A showed that incubation period on curative test was 8 days after inoculation (DAI) whereas on preventive and induced resistance test were 5 DAI. Disease severity at 10 DAI reached 4.8%, 23.20%, and 29.60% on curative, preventive, and induced resistance tests, respectively. Disease severity was 29.60% and 68.80% on positive control and negative control, respectively.

Formula Pelet Berbahan Aktif Trichoderma sp. dan Aplikasinya terhadap Penyakit Rebah Kecambah pada Tanaman Mentimun Bonny Poernomo Wahyu Soekarno; Surono Surono; Susanti Susanti
Jurnal Fitopatologi Indonesia Vol 10 No 5 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cucumber (Cucumis sativus) is an important vegetable comodity in Indonesia, but its production rate is still low. One of the factors that cause the low productivity is plant diseases. Damping-off disease often causes the damage of cucumber plant caused by a fungus that lives in the soil as soilborne disease, Phytium sp. The controlling effort of this disease have been done, for example using Trichoderma sp. as bio-agent, but the utilization of the bio-agent still is not practical so it is difficult to be applied in field. The pellet formula is more effective to be implemented because of the small size and easily moved. The purpose of this research was to produce the pellet formulation with active material of Trichoderma sp. with rich nutritious content, cheap and easy carrier. This research used two tests, in vitro and in vivo test. The in vitro test used six different pellet formulations, DAT, UAT, TAT, PAT, DDS and ATS. The best formulation in in vitro test are UAT and DDS, then the formulas were used in in vivo test. Both formulas could suppress the pathogenic fungus, Pythium, in vivo test.

Penghambatan Fusarium oxysporum oleh Kultur Filtrat Bakteri Endofit dari Tanaman Kedelai secara in Vitro Novi Malinda; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed borne pathogen play an important role as source of inoculum for disease incidence in the field and it becomes a major constraint in certified seed production. Research was conducted to isolate potential endophytic bacteria from soybean plants and evaluate its culture filtrate for inhibition effect of seedborne fungi on soybean seed, i.e. Fusarium oxysporum. The result showed that out of forty eight endophytic bacteria isolates that were nonpathogenic, there were three potential isolates that can inhibit the growth of F. oxysporum, i.e. EDA 3, EBA 6, and EBA 7 with percent inhibition of 60.14%, 57.69%, and 57.08%, respectively. The filtrate culture of EBA 7 showed the highest inhibition (34.88%) by in vitro test. Therefore, those three isolates of endophytic bacteria might be used as biocontrol agent to inhibit the growth of F. oxysporum.

Potensi Metabolit Sekunder Cendawan Endofit Tanaman Cabai sebagai Penghambat Fusarium sp. Patogen Asal Biji Secara in Vitro Dewi Novina Sukapiring; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol 12 No 1 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic fungi was known as controlling agents to pathogenic fungi, including Fusarium oxysporum. This research was aimed to select endophytic fungi from pepper which produced secondary metabolites and have beneficial effect in controlling seed borne pathogen especially Fusarium sp. Four isolates was obtained, i.e. CECL 19, CECL 28, CECL 38, and CECL 40; and further examined in 3 media fermentation, i.e. yeast glucose broth, potato dextrose broth, and potato dextrose yeast broth. Metabolites of endophytic fungi was tested in vitro for its inhibition effect on the growth of Fusarium sp. The result showed that the type of fermentation medium was significantly determining the ability of endophytic fungi in inhibiting the growth of Fusarium sp. Medium PDA and DEC was determined as the best medium to optimize metabolite production of CECL 28 and CECL 18, respectively. Metabolite compound produced by CECL 28 has been effective to inhibited Fusarium sp.

Metabolit Cendawan Endofit Tanaman Padi sebagai Alternatif Pengendalian Cendawan Patogen Terbawa Benih Padi Arifda Ayu Swastini Waruwu; Bonny Poernomo Wahyu Soekarno; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 12 No 2 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Infection of seed-borne fungi on rice (Oryza sativa) may affect rice production and becomes an important problem in Indonesia recently. This study aimed to evaluate the potential of fungal endophyte metabolites isolated from rice (Oryza sativa L.) to control seed-borne pathogenic fungi. Research activities involved isolation of endophytic fungi from rice, isolation of seed-borne fungi, and inhibition test of isolated endophytic fungal metabolites on seed-borne pathogenic fungi. The results showed that 3 isolates of endophytic fungi, i.e. LA6, LA11, and LA14 were potential producing antifungal metabolite. The metabolites of LA11 and LA14 isolates was able to reduce the growth of seed-borne pathogenic fungi between 18.33 and 47.28%.

Seleksi Bakteri Endofit Penghasil Senyawa Metabolit untuk Pengendalian Cendawan Patogen Terbawa Benih Jagung Andini Hanif; Bonny Poernomo Wahyu Soekarno; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic bacteria have been reported to produce metabolite as antifungal compound. This study was aimed to obtain endophytic bacteria which are able to produce metabolite to control Fusarium sp., a potential seedborne fungi on maize. Endophytic bacteria were screened by hypersensitive test on tobacco leaves and antagonistic test. Endophytic bacteria isolates with high growth inhibitor activity were selected and examined for their metabolite compound. Thre isolates, i.e. Lactobacillus sp. isolate EF14III, Pseudomonas sp. isolate ER1I, dan Aeromonas sp. isolate ER10I has the potential to inhibit Fusarium sp.. Metabolite compound of Pseudomonas sp. isolates ER1I was able to decrease the infection Fusarium sp. by 65.0% in blotter test and decreased infection of Fusarium sp. up to 59.5% and 60.5% in growing on test using water agar and sterile soil, respectively. Cyclohexanone with concentration of 9.68% produced by Pseudomonas sp. isolat ERI1 may play a role as antifungal compound.

Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus Siti Hardiyanti; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Rigidoporus lignosus is the most important pathogen of rubber tree which causes white root rot disease. The use of antagonistic microbe is recommended to control this pathogen. This research was conducted to isolate endophytic and rhizospheric microbes, and to study their ability to inhibit growth of R. lignosus. Research consisted of isolation of endophytic and rhizospheric microbes, pathogenicity test, in vitro and in vivo assays, growth promotion assays, and identification. There were 99 isolates of bacteria and 18 isolates of fungi isolated from the root and rhizosphere of rubber trees. In vitro and in vivo assay showed that 2 bacterial isolates, i.e. endophytic bacteria ME8, and rhizospheric bacteria MR3; and 3 fungal isolates, i.e. endophytic fungi CB8, CB6, and CL3 were able to inhibit the growth of R. lignosus. Endophytic bacteria ME8 showed the ability of solibilizing phosphate and fixing nitrogen. Rhizospheric bacteria MR3 showed the ability of solubilizing phosphate. The isolates CB6 and CL3 were very similar with Chaetomium sp. and Penicillium sp., respectively based on morphological characters; while CB8 was identified as mycelial sterile. Based on 16S rRNA sequences, endophytic bacterium ME8 and rhizospheric bacteria ME3 were identified as Bacillus siamensis B268 and B. amylolyquefaciens BCRh10, respectively. Endophytic and rhizospheric microbes isolated from rubber trees has the potency as biocontrol agents of R. lignosus.

Metabolit Bakteri Endofit Asal Tanaman Kacang Tanah sebagai Penghambat Pertumbuhan Aspergillus flavus Nela Zahara; Bonny Poernomo Wahyu Soekarno; Abdul Munif
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

eanut (Arachis hypogeae) is considered as important crop in Indonesia and domestic demands to peanut tend to increase recently. Infection of fungal seedborne, Aspergillus flavus, may downgrade peanut seed quality. Research was conducted to evaluate the application of secondary metabolite produced by endophytic bacteria for controlling A. flavus. The methodologies involved isolation A. flavus and endophytic bacteria from the plant tissue of peanut, hypersensitive test of endophytic bacteria, inhibition test of endophytic bacteria against A. flavus, extraction and examination of secondary metabolite from endophytic bacteria, seed treatment using extracted secondary metabolite, and molecular identification of the bacteria. Based on hypersensitive test, 37 isolates of endophytic bacteria were identified as non pathogenic. Further screening by dual culture test found 3 isolates with high inhibition activity, i.e. BE2B2-1 (71.64%), BE2B2-2 (69.05%), and BE2B2-5 (62.25%). Molecular identification based on nucleotide sequencing of 16S rRNA showed that BE2B2-1, BE2B2-2, and BE2B2-5 were Enterobacter sp., Bacillus sp., and Acinetobacter sp., respectively. Metabolite from Enterobacter sp. has the highest antimicrobial activity (61.70%) against A. flavus in in vitro test, highest infection inhibition (77.22%) in growing-on test, and highest increasing of seed germination rate (4.25%). This finding indicated the potential of secondary metabolites from endophyte bacteria to suppress infestation of A. flavus.

Co-Authors Abdul Munif Agus PURWANTARA Akhiruddin Maddu Andini Hanif Deden Dewantara ERIS Desni Roha Miriam Sakerebau Dewi Novina Sukapiring, Dewi Novina Djoko Pujiarto Dono Wahyuno Efi Toding Tondok Eva Marhaenis Gusmaini Gusmaini Jean Nihana Manalu KIKIN HAMZAH MUTAQIN Miftakhurohmah Miftakhurohmah Nela Zahara Nela Zahara Novi Malinda resky wulandari r jahuddin Siti Hardiyanti SRI HENDRASTUTI HIDAYAT Surono . Surono Surono Surono Surono Susanti Susanti Titiek Siti Yuliani Waruwu, Arifda Ayu Swastini

Title

Found 16 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 16 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 16 Documents
Search