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ERWAHYUNI ENDANG PRABANDARI
Graduated School of Agriculture Industrial Technology, Bogor Agricultural Institute
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Selection of Carbon and Nitrogen Source for 8-Hydroxy 9, 12-Octadecadienoic Acid Production using Endophytic Fungi Curvularia lunata BioMCC FE-00283 ERWAHYUNI ENDANG PRABANDARI; TUN TEDJA IRAWADI; WAHONO SUMARYONO; KHASWAR SYAMSU
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.109 KB) | DOI: 10.5454/mi.5.4.6

Abstract

Hydroxyoctadecadienoic acid (HODE) is one of hydroxy fatty acids that has anticancer activity.  HODE was previously produced by chemical synthesis or bioconversion from linoleic acid. This is the first paper reported production of HODE by Curvularia lunata an endophytic fungi of Cibotium barometz. Various carbon and hydrogen sources have been tested for their effects on the production of  HODE by C. lunata. Glucose, lactose, maltose, xylose, and sucrose were used as carbon sources, while yeast extract, monosodium glutamate, urea, and NH4Cl were used as nitrogen sources. Fermentation was done using 100 ml medium in 250 ml Erlenmeyer flask at 150 rpm, 28 oC for 10 days.  HODE products were analyzed by high pressure liquid chromatography using C18 coloumn and eluted by gradient system of acetonitril-water from 15% to 100%. Glucose  and monosodium glutamate were found to be the best carbon and nitrogen source.  The optimum concentration of glucose and monosodium glutamate for the production of HODE were 10 mg L-1 and 12 mg L-1 respectively.
Co-Authors Khaswar Syamsu Tun Tedja Irawadi Wahono Sumaryono