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All Journal Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI)
AHMAD WIBISANA
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Optimization of Surfactin Production by Bacillus amyloliquefaciens MD4-12 using Response Surface Methodology AHMAD WIBISANA; WAHONO SUMARYONO; MIRAWATI SUDIRO; PRATIWI PUDJILESTARI SUDARMONO
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.202 KB) | DOI: 10.5454/mi.9.3.4

Abstract

Surfactin is a lipopeptide biosurfactant that show potential biomedical application due to its activities such as antiviral, antibacterial, antifungi, anticancer, and antimycoplasma. Bacillus amyloliquefaciens MD4-12, isolated from oil-contaminated soil, produced promising yield of surfactin in McKeen medium. The production of surfactin was influenced by many fermentation process parameters such as carbon, nitrogen, minerals and also environmental conditions such as pH and agitation. Therefore, to obtain high yield of surfactin by Bacillus amyloliquefaciens MD4-12, optimization of process production was conducted in shake flask fermentation using response surface methodology. McKeen medium composition was used as basal medium.  Screening of the best carbon and nitrogen source were selected in preliminary experiments followed by selection of the influencing significant parameters on surfactin production using Plackett-Burman design. Selected parameters were optimized by central composite design and for the data analysis was used response surface methodology. The result showed that the optimum medium composition contained (g/L) 45.0 glucose, 6.33 urea, 1.0 monosodium glutamate, 1.85 MgSO4.7H2O, 0.4 KCl, 0.5 K2HPO4 and 0.5 mL trace elements. The surfactin yield at optimal condition was 1.25 g/L, increased 2.4 times compared to condition prior to optimization. 
OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum Lira Windriawati Listriyani; Sumi Hudiyono; Rudiyono Rudiyono; Siti Zulaeha; Ahmad Wibisana
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method.
Co-Authors Lira Windriawati Listriyani Mirawati Sudiro Pratiwi Pujilestari Sudarmono Rudiyono Rudiyono Siti Zulaeha Sumi Hudiyono Wahono Sumaryono