Nurul Pratiwi
Indonesian Research Institute for Animal Production

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Multivariate analysis of five chicken breed in Indonesia based on microsatellite allele frequency Ferdy Saputra; Tike Sartika; Anneke Anggraeni; Andi Baso Lompengeng Ishak; Komarudin Komarudin; Nurul Pratiwi
Livestock and Animal Research Vol 19, No 1 (2021): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.538 KB) | DOI: 10.20961/lar.v19i1.43459

Abstract

Objective: This study tries to examine several multivariate methods in classifying genetic diversity using microsatellite allele frequency data.Methods: This study used microsatellite allele frequency data from White Leghorn (n = 48), Kampung (n = 48), Pelung (n = 24), Sentul (n = 24), and Black Kedu (n = 25) from Indonesian Research Institute for Animal Production. Allele frequency data were analyzed by the Neighbor-Joining (NJ) method using the POPTREE2 program. The data was also analyzed by the Principal Component Analysis (PCA), Correspondence Analysis (CA), and Hierarchical Clustering on Principal Components (HCPC) methods using the factoextra and FactoMineR packages in the R 4.0.0 program.Results: Correspondence Analysis (CA) found Sentul is more closer to Black Kedu. However, based on NJ, PCA, and HCPC showed Sentul is closer to Kampung. Based on the value of Dimension 1, Correspondence Analysis (80.7%) can explain greater variation than PCA (58.9%). However, CA method generated different results compared to NJ, PCA, and HCPC. NJ, PCA, and HCPC found four chicken clusters, namely cluster 1 (White Leghorn), cluster 2 (Pelung), cluster 3 (Black Kedu), and cluster 4 (Kampung and Sentul).Conclusions: In conclusion, HCPC is a better multivariate method for analyzing allele frequency data than PCA and CA. HCPC can be used to analyze allele frequency data better than PCA, because HCPC is a combination of methods from hierarchical clustering and principal components.
Effectivity of Dry and Liquid BS4 Enzymes in Improving Performance of Broiler Chickens Fed Different Nutrient Density Diet Arnold Parlindungan Sinurat; Tuti Haryati; Nurul Pratiwi; Tresnawati Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 2 (2022): June 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i2.3051

Abstract

Supplementation of enzymes in feed is now commonly practiced to increase the nutrient availability of feed and the performance of poultry. A new enzyme called BS4 was produced by cultivating Eupenicilium javanicum. It is necessary to test the efficacy of this enzyme since the effectiveness of enzyme supplementation depends on many factors. An experiment was conducted to study the effect of dietary BS4 enzyme supplementation in improving the performance of broiler chickens. A number of 300 broilers DOC was distributed into 30 pens and reared until 35 d. Six experimental diets i.e., factorial of 2 (Standard diet, and low nutrient density diet) X 3 (Control, BS4 liquid enzyme, and BS4 powder enzyme) were formulated with 5 replications. The performance (feed intake, body weight, FCR, and survival rates) were observed during the starter (1-21 d) and whole (1-35 d) periods. At the end of the trial, measurements were also made on the carcass yield, abdominal fat, liver, and gizzard weights. Results showed that performances of broilers from 1-35 d were not significantly affected by interaction between nutrient density and enzyme supplement. The nutrient density also did not affect performances of broilers. However, dietary enzyme supplementation significantly reduced feed intake and improved FCR of broilers as compared to the control. Supplementation of BS4 in liquid or powder form, reduced feed intake by 3.6%. Supplementation of liquid and powder BS4 enzymes improved FCR by 6.4% and 8.9%, respectively, but no different effect between liquid and powder BS4 enzymes on performance of broilers. Nutrient density, enzyme supplementation, and interactions between the two factors did not significantly influence carcass yield, abdominal fat, liver, and gizzard relative weights of broilers.